NO960484L - Anvendelsen av exonuklease og/eller glykosylase som supplement til anti-polymerase-antistoff for å öke spesifisiteten i polymerasekjedereaksjon - Google Patents

Anvendelsen av exonuklease og/eller glykosylase som supplement til anti-polymerase-antistoff for å öke spesifisiteten i polymerasekjedereaksjon

Info

Publication number
NO960484L
NO960484L NO960484A NO960484A NO960484L NO 960484 L NO960484 L NO 960484L NO 960484 A NO960484 A NO 960484A NO 960484 A NO960484 A NO 960484A NO 960484 L NO960484 L NO 960484L
Authority
NO
Norway
Prior art keywords
polymerase
glycosylase
exonuclease
adjunct
chain reaction
Prior art date
Application number
NO960484A
Other languages
English (en)
Norwegian (no)
Other versions
NO960484D0 (no
Inventor
John William Hender Sutherland
David Robert Patterson
Original Assignee
Johnson & Johnson Clin Diag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Clin Diag filed Critical Johnson & Johnson Clin Diag
Publication of NO960484D0 publication Critical patent/NO960484D0/no
Publication of NO960484L publication Critical patent/NO960484L/no

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/30Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/319Exonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/531Glycosylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)
NO960484A 1995-02-07 1996-02-06 Anvendelsen av exonuklease og/eller glykosylase som supplement til anti-polymerase-antistoff for å öke spesifisiteten i polymerasekjedereaksjon NO960484L (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US38501995A 1995-02-07 1995-02-07

Publications (2)

Publication Number Publication Date
NO960484D0 NO960484D0 (no) 1996-02-06
NO960484L true NO960484L (no) 1996-08-08

Family

ID=23519709

Family Applications (1)

Application Number Title Priority Date Filing Date
NO960484A NO960484L (no) 1995-02-07 1996-02-06 Anvendelsen av exonuklease og/eller glykosylase som supplement til anti-polymerase-antistoff for å öke spesifisiteten i polymerasekjedereaksjon

Country Status (15)

Country Link
US (1) US5985619A (fr)
EP (1) EP0726324B1 (fr)
JP (1) JPH08266298A (fr)
KR (1) KR960031627A (fr)
AT (1) ATE201452T1 (fr)
AU (1) AU709788B2 (fr)
CA (1) CA2168712A1 (fr)
DE (1) DE69612885T2 (fr)
DK (1) DK0726324T3 (fr)
ES (1) ES2156978T3 (fr)
FI (1) FI960540A (fr)
GR (1) GR3036391T3 (fr)
NO (1) NO960484L (fr)
PT (1) PT726324E (fr)
SI (1) SI0726324T1 (fr)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6994960B1 (en) * 1997-05-28 2006-02-07 The Walter And Eliza Hall Institute Of Medical Research Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage
US6300075B1 (en) * 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
FR2803601A1 (fr) * 2000-01-11 2001-07-13 Inst Nat Sante Rech Med Utilisation de l'exonuclease iii dans des reactions d'amplification par polymerase de fragments d'adn
EP1275735A1 (fr) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition et méthode pour l'amplification des acides nucléiques à la facon 'hot start'
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
EP1953246A3 (fr) 2003-05-01 2008-11-19 Veridex, LLC Analyse de nýud lymphoïde intraopératif
US20090298052A1 (en) * 2004-06-04 2009-12-03 David Atkins Diagnosing or Predicting the Course of Breast Cancer
US20070154889A1 (en) * 2004-06-25 2007-07-05 Veridex, Llc Methods and reagents for the detection of melanoma
ATE510027T1 (de) * 2006-02-27 2011-06-15 Hoffmann La Roche Pcr-heissstart durch magnesium-sequestrierung
EP3167900B1 (fr) 2006-03-29 2018-11-21 Merial Limited Vaccin contre des streptocoques
CA2658520C (fr) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Amplification d'acide nucleique en presence d'oligonucleotides randomises modifies
EP2990487A1 (fr) 2008-05-08 2016-03-02 Asuragen, INC. Compositions et procédés relatifs à la modulation de miarn de néovascularisation ou angiogenèse
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
EP2432895A2 (fr) 2009-05-22 2012-03-28 Asuragen, INC. Biomarqueurs miarn d'une maladie prostatique
IL206810A (en) 2009-07-08 2015-07-30 Janssen Diagnostics Llc Methods for identifying melanoma and kits for using these methods
US8735074B2 (en) 2009-08-28 2014-05-27 Asuragen, Inc. MiRNA biomarkers of lung disease
WO2012068400A2 (fr) 2010-11-17 2012-05-24 Asuragen, Inc. Micro-arn utilisés comme biomarqueurs pour faire la distinction entre des néoplasmes thyroïdiens bénins et malins
WO2014145612A1 (fr) 2013-03-15 2014-09-18 Ajay Goel Biomarqueurs de miarn à base de tissu et de sang pour le diagnostic, le pronostic et le potentiel prédictif de métastases dans le cancer colorectal
CA2905949A1 (fr) 2013-03-15 2014-09-25 Baylor Research Institute Marqueurs d'une neoplasie colorectale associee a la colite ulcereuse (uc)
JP6706208B2 (ja) 2014-01-05 2020-06-03 バイオマーナ ホールディングス リミテッド Mirna比率を使用する肺がん決定
WO2015120382A1 (fr) 2014-02-07 2015-08-13 The Johns Hopkins University Prédiction de réponse à un traitement utilisant des médicaments épigénétiques
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
WO2016094330A2 (fr) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Procédés et systèmes d'apprentissage par machine pour prédire la probabilité ou le risque d'avoir le cancer
WO2016177774A1 (fr) 2015-05-04 2016-11-10 Academisch Medisch Centrum Procédé de quantification des mi-arn à l'aide de la normalisation
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
JP2019198259A (ja) * 2018-05-15 2019-11-21 株式会社島津製作所 1ステップrt−pcr法
WO2020172362A1 (fr) * 2019-02-20 2020-08-27 Genetics Research, Llc Réactions multiplex régulées dans un seul tube
EP3719144A1 (fr) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p en tant que contrôle endogène universel pour la normalisation d'un chargement d'exosomes
WO2021013592A1 (fr) 2019-07-19 2021-01-28 Fundación Para La Investigación Biomédica Del Hospital Universitario La Paz (Fibhulp) Procédé de détermination de la réponse au traitement d'un patient atteint d'un carcinome pulmonaire non à petites cellules (nsclc)
CN114787193B (zh) * 2019-12-19 2023-04-21 深圳华大生命科学研究院 抗聚合酶的单克隆抗体及其应用
WO2023146243A1 (fr) * 2022-01-26 2023-08-03 주식회사 씨젠 Procédé de détection d'acides nucléiques cibles dans un échantillon
CN116773291B (zh) * 2023-03-31 2024-04-19 艾普拜生物科技(苏州)有限公司 一种石蜡切片dna预处理试剂及方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE176002T1 (de) * 1990-07-24 1999-02-15 Hoffmann La Roche Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
JP2985446B2 (ja) * 1990-10-31 1999-11-29 東ソー株式会社 核酸の検出及び測定方法
CA2101119A1 (fr) * 1992-08-17 1994-02-18 George T. Walker Methode de decontamination a l'exonuclease
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5605796A (en) * 1994-07-19 1997-02-25 Behringwerke Ag Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates

Also Published As

Publication number Publication date
EP0726324A2 (fr) 1996-08-14
DK0726324T3 (da) 2001-07-30
JPH08266298A (ja) 1996-10-15
US5985619A (en) 1999-11-16
DE69612885D1 (de) 2001-06-28
SI0726324T1 (en) 2001-12-31
ATE201452T1 (de) 2001-06-15
AU4339196A (en) 1996-08-15
CA2168712A1 (fr) 1996-08-08
EP0726324A3 (fr) 1996-11-27
ES2156978T3 (es) 2001-08-01
GR3036391T3 (en) 2001-11-30
EP0726324B1 (fr) 2001-05-23
FI960540A0 (fi) 1996-02-06
DE69612885T2 (de) 2001-09-13
NO960484D0 (no) 1996-02-06
AU709788B2 (en) 1999-09-09
KR960031627A (ko) 1996-09-17
FI960540A (fi) 1996-08-08
PT726324E (pt) 2001-08-30

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