CA2198470A1 - Methode de marquage de la luciferase - Google Patents
Methode de marquage de la luciferaseInfo
- Publication number
- CA2198470A1 CA2198470A1 CA002198470A CA2198470A CA2198470A1 CA 2198470 A1 CA2198470 A1 CA 2198470A1 CA 002198470 A CA002198470 A CA 002198470A CA 2198470 A CA2198470 A CA 2198470A CA 2198470 A1 CA2198470 A1 CA 2198470A1
- Authority
- CA
- Canada
- Prior art keywords
- luciferase
- antibody
- adenosine triphosphate
- chemical entity
- luciferin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108060001084 Luciferase Proteins 0.000 title abstract 8
- 239000005089 Luciferase Substances 0.000 title abstract 8
- 238000002372 labelling Methods 0.000 title 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 abstract 4
- 150000005829 chemical entities Chemical class 0.000 abstract 4
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 abstract 3
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 abstract 3
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 abstract 3
- 239000011230 binding agent Substances 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 238000005859 coupling reaction Methods 0.000 abstract 3
- 229910001425 magnesium ion Inorganic materials 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 230000009870 specific binding Effects 0.000 abstract 3
- 239000000427 antigen Substances 0.000 abstract 2
- 108091007433 antigens Proteins 0.000 abstract 2
- 102000036639 antigens Human genes 0.000 abstract 2
- 230000008878 coupling Effects 0.000 abstract 2
- 238000010168 coupling process Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 238000003149 assay kit Methods 0.000 abstract 1
- 230000027455 binding Effects 0.000 abstract 1
- 230000001268 conjugating effect Effects 0.000 abstract 1
- YGXMYKTZDMYISM-IDIVVRGQSA-J dimagnesium;[[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] phosphate Chemical compound [Mg+2].[Mg+2].C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O YGXMYKTZDMYISM-IDIVVRGQSA-J 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- -1 m antibody Substances 0.000 abstract 1
- 229910052749 magnesium Inorganic materials 0.000 abstract 1
- 239000011777 magnesium Substances 0.000 abstract 1
- 229960002261 magnesium phosphate Drugs 0.000 abstract 1
- 239000002777 nucleoside Substances 0.000 abstract 1
- 150000003833 nucleoside derivatives Chemical class 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 238000000159 protein binding assay Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9417593A GB9417593D0 (en) | 1994-09-01 | 1994-09-01 | Luciferase labelling method |
| GB9417593.2 | 1994-09-01 | ||
| PCT/GB1995/002038 WO1996007100A2 (fr) | 1994-09-01 | 1995-08-30 | Technique de marquage a la luciferase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2198470A1 true CA2198470A1 (fr) | 1996-03-07 |
| CA2198470C CA2198470C (fr) | 2006-02-14 |
Family
ID=35892302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002198470A Expired - Fee Related CA2198470C (fr) | 1994-09-01 | 1995-08-30 | Methode de marquage de la luciferase |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2198470C (fr) |
-
1995
- 1995-08-30 CA CA002198470A patent/CA2198470C/fr not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2198470C (fr) | 2006-02-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |