NO884847L - Polykationiske baerere for rensing av nukleinsyrer. - Google Patents
Polykationiske baerere for rensing av nukleinsyrer.Info
- Publication number
- NO884847L NO884847L NO884847A NO884847A NO884847L NO 884847 L NO884847 L NO 884847L NO 884847 A NO884847 A NO 884847A NO 884847 A NO884847 A NO 884847A NO 884847 L NO884847 L NO 884847L
- Authority
- NO
- Norway
- Prior art keywords
- stated
- probe
- polynucleotides
- particles
- separation
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract description 23
- 102000039446 nucleic acids Human genes 0.000 title abstract description 20
- 108020004707 nucleic acids Proteins 0.000 title abstract description 20
- 239000000969 carrier Substances 0.000 title description 11
- 238000004140 cleaning Methods 0.000 title 1
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- 238000000034 method Methods 0.000 claims abstract description 172
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- 239000002773 nucleotide Substances 0.000 claims abstract description 129
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 129
- 239000007787 solid Substances 0.000 claims abstract description 101
- 238000000926 separation method Methods 0.000 claims abstract description 85
- 230000027455 binding Effects 0.000 claims abstract description 45
- 238000003556 assay Methods 0.000 claims abstract description 44
- 238000000746 purification Methods 0.000 claims abstract description 26
- 239000000356 contaminant Substances 0.000 claims abstract description 17
- 239000000243 solution Substances 0.000 claims description 165
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- 239000002245 particle Substances 0.000 claims description 39
- 125000002091 cationic group Chemical group 0.000 claims description 37
- 238000001514 detection method Methods 0.000 claims description 31
- -1 phosphate diesters Chemical class 0.000 claims description 27
- 238000010828 elution Methods 0.000 claims description 26
- 239000011521 glass Substances 0.000 claims description 16
- 229910019142 PO4 Inorganic materials 0.000 claims description 15
- 239000010452 phosphate Substances 0.000 claims description 15
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- 229920000151 polyglycol Polymers 0.000 claims description 11
- 239000010695 polyglycol Substances 0.000 claims description 11
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- 239000005017 polysaccharide Substances 0.000 claims description 11
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims description 10
- 239000004952 Polyamide Substances 0.000 claims description 10
- 230000015572 biosynthetic process Effects 0.000 claims description 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 10
- 229920002647 polyamide Polymers 0.000 claims description 10
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical group CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 claims description 6
- 238000011084 recovery Methods 0.000 claims description 6
- 150000004982 aromatic amines Chemical class 0.000 claims description 5
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- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims description 4
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims description 4
- 239000000467 phytic acid Substances 0.000 claims description 4
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- 235000019832 sodium triphosphate Nutrition 0.000 claims description 4
- UNXRWKVEANCORM-UHFFFAOYSA-I triphosphate(5-) Chemical compound [O-]P([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O UNXRWKVEANCORM-UHFFFAOYSA-I 0.000 claims description 4
- 125000000217 alkyl group Chemical group 0.000 claims description 2
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- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 32
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- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 23
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- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 15
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- MBYLVOKEDDQJDY-UHFFFAOYSA-N tris(2-aminoethyl)amine Chemical compound NCCN(CCN)CCN MBYLVOKEDDQJDY-UHFFFAOYSA-N 0.000 description 11
- NNARPPSIYMCXMB-UHFFFAOYSA-N 1,4-bis(2-methylpropoxy)-1,4-dioxobutane-2-sulfonic acid Chemical compound CC(C)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(C)C NNARPPSIYMCXMB-UHFFFAOYSA-N 0.000 description 10
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
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- QRXMUCSWCMTJGU-UHFFFAOYSA-L (5-bromo-4-chloro-1h-indol-3-yl) phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP([O-])(=O)[O-])=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-L 0.000 description 7
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- FSVCQIDHPKZJSO-UHFFFAOYSA-L nitro blue tetrazolium dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 FSVCQIDHPKZJSO-UHFFFAOYSA-L 0.000 description 7
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- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 6
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- Microbiology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Detergent Compositions (AREA)
- Cleaning Or Drying Semiconductors (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Cleaning And De-Greasing Of Metallic Materials By Chemical Methods (AREA)
- Extraction Or Liquid Replacement (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US2086687A | 1987-03-02 | 1987-03-02 | |
PCT/US1988/000550 WO1988006633A1 (fr) | 1987-03-02 | 1988-03-02 | Supports polycationiques pour la purification, la separation et l'hybridation d'acides nucleiques |
Publications (2)
Publication Number | Publication Date |
---|---|
NO884847D0 NO884847D0 (no) | 1988-10-31 |
NO884847L true NO884847L (no) | 1988-12-22 |
Family
ID=21801014
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO884847A NO884847L (no) | 1987-03-02 | 1988-10-31 | Polykationiske baerere for rensing av nukleinsyrer. |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0281390B1 (fr) |
JP (1) | JP2862547B2 (fr) |
KR (1) | KR960000479B1 (fr) |
AT (1) | ATE107654T1 (fr) |
CA (1) | CA1339446C (fr) |
DE (1) | DE3850273T2 (fr) |
DK (1) | DK607588A (fr) |
ES (1) | ES2054797T3 (fr) |
FI (1) | FI885022A (fr) |
NO (1) | NO884847L (fr) |
PT (1) | PT86881A (fr) |
WO (1) | WO1988006633A1 (fr) |
Families Citing this family (86)
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DE68913555T2 (de) * | 1988-11-21 | 1994-07-07 | Dynal As | Sonden aus nukleinsäuren. |
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CA2012984A1 (fr) * | 1989-03-27 | 1990-09-27 | Frank W. Hobbs Jr. | Procede pour la detection rapide de l'acide nucleique par incorporation d'un groupe marqueur dans l'acide nucleique detecte par amplification de la cible, et ensuite par capture par affinite |
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DE19736366A1 (de) | 1997-08-21 | 1999-02-25 | Ernst Prof Dr Bayer | Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln |
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CN106148512B (zh) | 2011-04-15 | 2020-07-10 | 贝克顿·迪金森公司 | 扫描实时微流体热循环仪和用于同步的热循环和扫描光学检测的方法 |
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RU2622432C2 (ru) | 2011-09-30 | 2017-06-15 | Бектон, Дикинсон Энд Компани | Унифицированная полоска для реактивов |
CN104040238B (zh) | 2011-11-04 | 2017-06-27 | 汉迪拉布公司 | 多核苷酸样品制备装置 |
JP6262152B2 (ja) | 2012-02-03 | 2018-01-17 | ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company | 分子診断試験の分布及び試験間のコンパチビリティ判断のための外部ファイル |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
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NL8203102A (nl) * | 1982-08-04 | 1984-03-01 | Katholieke Universiteit Facult | Papier voor rna-blotting; werkwijze voor het maken daarvan; toepassingen van het papier. |
US4554088A (en) * | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
US4672040A (en) * | 1983-05-12 | 1987-06-09 | Advanced Magnetics, Inc. | Magnetic particles for use in separations |
US4652517A (en) * | 1984-06-11 | 1987-03-24 | Diagnostic Research Limited Partnership | Methods for the in vitro detection and identification of unknown pathogens or genetic entities |
EP0189280A3 (fr) * | 1985-01-23 | 1987-01-07 | Dekalb-Pfizer Genetics | Essai qualitatif pour les acides nucléiques |
EP0639582B1 (fr) * | 1985-03-15 | 1998-09-16 | Antivirals Inc. | Réactif et procédé d'analyse de polynucléotides |
US4806631A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on solvolyzed nylon supports |
US4806546A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on derivatized nylon supports |
-
1988
- 1988-03-02 JP JP63502502A patent/JP2862547B2/ja not_active Expired - Fee Related
- 1988-03-02 DE DE3850273T patent/DE3850273T2/de not_active Expired - Lifetime
- 1988-03-02 EP EP88301839A patent/EP0281390B1/fr not_active Expired - Lifetime
- 1988-03-02 ES ES88301839T patent/ES2054797T3/es not_active Expired - Lifetime
- 1988-03-02 AT AT88301839T patent/ATE107654T1/de not_active IP Right Cessation
- 1988-03-02 KR KR1019880701387A patent/KR960000479B1/ko not_active IP Right Cessation
- 1988-03-02 PT PT86881A patent/PT86881A/pt not_active Application Discontinuation
- 1988-03-02 WO PCT/US1988/000550 patent/WO1988006633A1/fr active Application Filing
- 1988-03-02 CA CA000560293A patent/CA1339446C/fr not_active Expired - Lifetime
- 1988-10-31 NO NO884847A patent/NO884847L/no unknown
- 1988-11-01 DK DK607588A patent/DK607588A/da not_active Application Discontinuation
- 1988-11-01 FI FI885022A patent/FI885022A/fi not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
AU1426988A (en) | 1988-09-26 |
DK607588A (da) | 1988-12-28 |
JPH01502319A (ja) | 1989-08-17 |
NO884847D0 (no) | 1988-10-31 |
DK607588D0 (da) | 1988-11-01 |
FI885022A0 (fi) | 1988-11-01 |
EP0281390B1 (fr) | 1994-06-22 |
WO1988006633A1 (fr) | 1988-09-07 |
CA1339446C (fr) | 1997-09-09 |
DE3850273T2 (de) | 1994-09-29 |
PT86881A (pt) | 1989-03-30 |
KR960000479B1 (ko) | 1996-01-08 |
ATE107654T1 (de) | 1994-07-15 |
DE3850273D1 (de) | 1994-07-28 |
EP0281390A2 (fr) | 1988-09-07 |
EP0281390A3 (en) | 1990-04-25 |
JP2862547B2 (ja) | 1999-03-03 |
FI885022A (fi) | 1988-11-01 |
ES2054797T3 (es) | 1994-08-16 |
KR890700682A (ko) | 1989-04-26 |
AU618951B2 (en) | 1992-01-16 |
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