CA2012984A1 - Procede pour la detection rapide de l'acide nucleique par incorporation d'un groupe marqueur dans l'acide nucleique detecte par amplification de la cible, et ensuite par capture par affinite - Google Patents

Procede pour la detection rapide de l'acide nucleique par incorporation d'un groupe marqueur dans l'acide nucleique detecte par amplification de la cible, et ensuite par capture par affinite

Info

Publication number
CA2012984A1
CA2012984A1 CA002012984A CA2012984A CA2012984A1 CA 2012984 A1 CA2012984 A1 CA 2012984A1 CA 002012984 A CA002012984 A CA 002012984A CA 2012984 A CA2012984 A CA 2012984A CA 2012984 A1 CA2012984 A1 CA 2012984A1
Authority
CA
Canada
Prior art keywords
nucleic acid
reporter moiety
assay
primer
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002012984A
Other languages
English (en)
Inventor
Frank W. Hobbs Jr.
Gerald J. Litt
Jeffrey A. Miller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CA2012984A1 publication Critical patent/CA2012984A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA002012984A 1989-03-27 1990-03-23 Procede pour la detection rapide de l'acide nucleique par incorporation d'un groupe marqueur dans l'acide nucleique detecte par amplification de la cible, et ensuite par capture par affinite Abandoned CA2012984A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32914289A 1989-03-27 1989-03-27
US329,142 1989-03-27

Publications (1)

Publication Number Publication Date
CA2012984A1 true CA2012984A1 (fr) 1990-09-27

Family

ID=23284035

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002012984A Abandoned CA2012984A1 (fr) 1989-03-27 1990-03-23 Procede pour la detection rapide de l'acide nucleique par incorporation d'un groupe marqueur dans l'acide nucleique detecte par amplification de la cible, et ensuite par capture par affinite

Country Status (5)

Country Link
EP (1) EP0465557A1 (fr)
JP (1) JPH04504202A (fr)
AU (1) AU5354790A (fr)
CA (1) CA2012984A1 (fr)
WO (1) WO1990011373A1 (fr)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
KR960000479B1 (ko) * 1987-03-02 1996-01-08 젠-프로우브 인코오퍼레이티드 핵산 정제,분리 및 혼성체 형성용 다가양이온성 지지체
CA1317535C (fr) * 1987-06-30 1993-05-11 Nanibhushan Dattagupta Essai de sequences a l'aide de genes amplifies

Also Published As

Publication number Publication date
JPH04504202A (ja) 1992-07-30
WO1990011373A1 (fr) 1990-10-04
EP0465557A1 (fr) 1992-01-15
AU5354790A (en) 1990-10-22

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Legal Events

Date Code Title Description
FZDE Dead