NO850493L - Heterologt paavisningssystem - Google Patents
Heterologt paavisningssystemInfo
- Publication number
- NO850493L NO850493L NO850493A NO850493A NO850493L NO 850493 L NO850493 L NO 850493L NO 850493 A NO850493 A NO 850493A NO 850493 A NO850493 A NO 850493A NO 850493 L NO850493 L NO 850493L
- Authority
- NO
- Norway
- Prior art keywords
- biotinylated
- lectin
- enzyme
- complex
- streptavidin
- Prior art date
Links
- 239000002523 lectin Substances 0.000 claims abstract description 75
- 108090001090 Lectins Proteins 0.000 claims abstract description 74
- 102000004856 Lectins Human genes 0.000 claims abstract description 74
- 102000004190 Enzymes Human genes 0.000 claims abstract description 65
- 108090000790 Enzymes Proteins 0.000 claims abstract description 65
- 108020004414 DNA Proteins 0.000 claims abstract description 63
- 108090001008 Avidin Proteins 0.000 claims abstract description 37
- 229920002307 Dextran Polymers 0.000 claims abstract description 29
- 108090000288 Glycoproteins Proteins 0.000 claims abstract description 24
- 102000003886 Glycoproteins Human genes 0.000 claims abstract description 24
- 108010090804 Streptavidin Proteins 0.000 claims abstract description 23
- 102000053602 DNA Human genes 0.000 claims abstract description 10
- 150000004676 glycans Chemical class 0.000 claims abstract description 8
- 125000003147 glycosyl group Chemical group 0.000 claims abstract description 8
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 8
- 239000005017 polysaccharide Substances 0.000 claims abstract description 8
- 150000001720 carbohydrates Chemical class 0.000 claims abstract 7
- 238000000034 method Methods 0.000 claims description 19
- 239000002773 nucleotide Substances 0.000 claims description 16
- 125000003729 nucleotide group Chemical group 0.000 claims description 16
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 41
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract description 40
- 239000011616 biotin Substances 0.000 abstract description 32
- 229960002685 biotin Drugs 0.000 abstract description 32
- 238000009739 binding Methods 0.000 abstract description 20
- 235000020958 biotin Nutrition 0.000 abstract description 20
- 108010062580 Concanavalin A Proteins 0.000 abstract description 17
- 108010001336 Horseradish Peroxidase Proteins 0.000 abstract description 16
- 235000000346 sugar Nutrition 0.000 abstract description 14
- 239000000463 material Substances 0.000 abstract description 11
- 238000006243 chemical reaction Methods 0.000 abstract description 7
- 239000012620 biological material Substances 0.000 abstract description 6
- 102000003992 Peroxidases Human genes 0.000 abstract description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract description 3
- 230000000295 complement effect Effects 0.000 abstract description 3
- HBOMLICNUCNMMY-KJFJCRTCSA-N 1-[(4s,5s)-4-azido-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1C1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-KJFJCRTCSA-N 0.000 abstract description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 abstract description 2
- 102000013563 Acid Phosphatase Human genes 0.000 abstract description 2
- 108010051457 Acid Phosphatase Proteins 0.000 abstract description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract description 2
- 229940088598 enzyme Drugs 0.000 description 54
- 230000027455 binding Effects 0.000 description 19
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 12
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 12
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 12
- 239000011324 bead Substances 0.000 description 10
- 239000000758 substrate Substances 0.000 description 10
- 239000000872 buffer Substances 0.000 description 9
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- 229930186217 Glycolipid Natural products 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 239000013077 target material Substances 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 239000000020 Nitrocellulose Substances 0.000 description 4
- 229920002684 Sepharose Polymers 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 229920001220 nitrocellulos Polymers 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 2
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000013504 Triton X-100 Substances 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 239000011149 active material Substances 0.000 description 2
- WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000010804 cDNA synthesis Methods 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 239000000787 lecithin Substances 0.000 description 2
- 229940067606 lecithin Drugs 0.000 description 2
- 235000010445 lecithin Nutrition 0.000 description 2
- 108010034897 lentil lectin Proteins 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 230000009871 nonspecific binding Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 102000006386 Myelin Proteins Human genes 0.000 description 1
- 108010083674 Myelin Proteins Proteins 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- LEBBDRXHHNYZIA-LDUWYPJVSA-N [(2s,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] n-[(z)-1,3-dihydroxyoctadec-4-en-2-yl]carbamate Chemical compound CCCCCCCCCCCCC\C=C/C(O)C(CO)NC(=O)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O LEBBDRXHHNYZIA-LDUWYPJVSA-N 0.000 description 1
- 108010070626 acid beta-galactosidase Proteins 0.000 description 1
- WQZGKKKJIJFFOK-PQMKYFCFSA-N alpha-D-mannose Chemical compound OC[C@H]1O[C@H](O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-PQMKYFCFSA-N 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000006287 biotinylation Effects 0.000 description 1
- 238000007413 biotinylation Methods 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- 108010039433 dolichos biflorus agglutinin Proteins 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 150000002270 gangliosides Chemical class 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000005012 myelin Anatomy 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 150000003641 trioses Chemical class 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/824—Immunological separation techniques
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/827—Lectins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
- Controls And Circuits For Display Device (AREA)
- Control Of Indicators Other Than Cathode Ray Tubes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US57873284A | 1984-02-09 | 1984-02-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO850493L true NO850493L (no) | 1985-08-12 |
Family
ID=24314075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO850493A NO850493L (no) | 1984-02-09 | 1985-02-08 | Heterologt paavisningssystem |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US4889798A (ja) |
| EP (1) | EP0151492B1 (ja) |
| JP (1) | JPS60188095A (ja) |
| AT (1) | ATE133261T1 (ja) |
| AU (1) | AU3844485A (ja) |
| CA (1) | CA1314810C (ja) |
| DE (1) | DE3588078T2 (ja) |
| DK (1) | DK61085A (ja) |
| ES (2) | ES8802188A1 (ja) |
| IL (1) | IL74262A0 (ja) |
| NO (1) | NO850493L (ja) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4957858A (en) * | 1986-04-16 | 1990-09-18 | The Salk Instute For Biological Studies | Replicative RNA reporter systems |
| DE3587845T2 (de) * | 1984-12-03 | 1994-10-20 | Hoechst Celanese Corp | Verfahren zur Bestimmung eines Liganden. |
| US5273882A (en) * | 1985-06-13 | 1993-12-28 | Amgen | Method and kit for performing nucleic acid hybridization assays |
| IL79112A (en) * | 1985-06-13 | 1992-01-15 | Abbott Lab | Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay |
| US4724203A (en) * | 1985-10-04 | 1988-02-09 | Miles Laboratories, Inc. | Caproylamidobiotinylated peroxidase |
| US5817797A (en) * | 1988-06-01 | 1998-10-06 | The United States Of America As Represented By The Department Of Health And Human Services | Sequencing DNA; a modification of the polymerase chain reaction |
| CA2002076A1 (en) * | 1988-11-21 | 1990-05-21 | Brent A. Burdick | Diagnostic kit and method using a solid phase capture means for detecting nucleic acids |
| GB2239948B (en) * | 1989-12-28 | 1994-01-12 | Aisin Seiki | Fluorescence assay using phosphatase and a naphthol phosphate derivative |
| JP2646814B2 (ja) * | 1990-08-07 | 1997-08-27 | アイシン精機株式会社 | 核酸等の検定方法 |
| US5264343A (en) * | 1990-08-31 | 1993-11-23 | Eleanor Roosevelt Institute | Method for distinguishing normal and cancer cells |
| EP0648281A4 (en) * | 1991-09-10 | 1997-06-04 | Jack D Love | TARGETED AMPLIFICATION OF DNA AND RNA AND SIGNAL AMPLIFICATION. |
| US5329461A (en) * | 1992-07-23 | 1994-07-12 | Acrogen, Inc. | Digital analyte detection system |
| JPH07165800A (ja) * | 1993-12-08 | 1995-06-27 | Res Dev Corp Of Japan | 人工蛋白質超分子とその製造法 |
| US5783387A (en) * | 1995-02-06 | 1998-07-21 | The Regents Of The University Of California | Method for identifying and quantifying nucleic acid sequence aberrations |
| US5731153A (en) * | 1996-08-26 | 1998-03-24 | The Regents Of The University Of California | Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions |
| US6096508A (en) * | 1995-08-16 | 2000-08-01 | Kirkegaard & Perry Laboratoies, Inc. | Method of reducing background in biotin-based assays |
| DE19724787A1 (de) * | 1997-06-06 | 1998-12-10 | Biotez Berlin Buch Gmbh Bioche | Streptavidin/Avidin beschichtete Oberflächen |
| JP2002508190A (ja) * | 1997-12-12 | 2002-03-19 | ダイジーン・コーポレーション | 万能収集媒質 |
| KR20000074881A (ko) * | 1999-05-26 | 2000-12-15 | 황승용 | 마이크로웰을 이용한 디엔에이 돌연변이의 확인방법 및 키트 |
| JP3781934B2 (ja) * | 1999-12-22 | 2006-06-07 | 株式会社ニチレイバイオサイエンス | 酵素−タンパク質複合体 |
| US7102024B1 (en) | 2000-08-01 | 2006-09-05 | Schwartz David A | Functional biopolymer modification reagents and uses thereof |
| US6686461B1 (en) | 2000-03-22 | 2004-02-03 | Solulink Bioscience, Inc. | Triphosphate oligonucleotide modification reagents and uses thereof |
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| US7601497B2 (en) * | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| US8574833B2 (en) | 2007-11-28 | 2013-11-05 | Great Basin Scientific | Methods and compositions for amplifying a detectable signal |
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| CA2750338C (en) * | 2009-01-28 | 2019-06-25 | Qiagen Gaithersburg, Inc. | Sequence-specific large volume sample preparation method and assay |
| CN102414327B (zh) | 2009-05-01 | 2015-04-08 | 奇亚根盖瑟斯堡股份有限公司 | 用于在样品中检测rna剪接形式的非靶向扩增方法 |
| CA2773320C (en) | 2009-09-14 | 2018-02-20 | Qiagen Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
| US9605303B2 (en) | 2010-01-29 | 2017-03-28 | Qiagen Gaithersburg, Inc. | Method of determining and confirming the presence of an HPV in a sample |
| US9689047B2 (en) * | 2010-01-29 | 2017-06-27 | Qiagen Gaithersburg Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
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Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| JPS5510590A (en) * | 1978-05-04 | 1980-01-25 | Wellcome Found | Enzyme immunity quantity analysis |
| US4334017A (en) * | 1979-04-16 | 1982-06-08 | Massachusetts Institute Of Technology | Method for detecting cancer in mammalian tissue |
| US4478914B1 (en) * | 1980-01-24 | 1997-06-17 | Roger W Giese | Process for applying multiple layers of a protein and a ligand extender to a surface and to the multiple layer system |
| CA1219824A (en) * | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
| US4395486A (en) * | 1981-08-19 | 1983-07-26 | Medical College Of Ga. Research Inst., Inc. | Method for the direct analysis of sickle cell anemia |
| US4455380A (en) * | 1982-01-29 | 1984-06-19 | Otsuka Pharmaceutical Co., Ltd. | Process for determining tumor-associated glycolinkage |
| CA1254525A (en) * | 1983-04-13 | 1989-05-23 | Christine L. Brakel | Kit for terminally chemically labeling dna |
| DK188184A (da) * | 1983-04-20 | 1984-10-21 | Enzo Biochem Inc | Fremgangsmaade til dannelse af et kompleks af biologisk aktive eller funktionelle forbindelser og anvendelse af forbindelserne |
| CA1228811A (en) * | 1983-05-05 | 1987-11-03 | Robert G. Pergolizzi | Assay method utilizing polynucleotide sequences |
| US4687732A (en) * | 1983-06-10 | 1987-08-18 | Yale University | Visualization polymers and their application to diagnostic medicine |
| US4550075A (en) * | 1983-06-22 | 1985-10-29 | Kallestad Laboratories, Inc. | Method for ligand determination utilizing an immunoassay monitorable by biotin-containing enzymes, and compositions therefor |
| WO1985003356A1 (en) * | 1984-01-27 | 1985-08-01 | Molecular Biosystems, Inc. | Assay for immobilized reporter groups |
-
1985
- 1985-02-05 AU AU38444/85A patent/AU3844485A/en not_active Abandoned
- 1985-02-06 IL IL74262A patent/IL74262A0/xx unknown
- 1985-02-07 JP JP60020996A patent/JPS60188095A/ja active Pending
- 1985-02-07 CA CA000473830A patent/CA1314810C/en not_active Expired - Lifetime
- 1985-02-08 ES ES540250A patent/ES8802188A1/es not_active Expired
- 1985-02-08 DK DK61085A patent/DK61085A/da not_active Application Discontinuation
- 1985-02-08 AT AT85101353T patent/ATE133261T1/de not_active IP Right Cessation
- 1985-02-08 EP EP85101353A patent/EP0151492B1/en not_active Expired - Lifetime
- 1985-02-08 NO NO850493A patent/NO850493L/no unknown
- 1985-02-08 DE DE3588078T patent/DE3588078T2/de not_active Expired - Lifetime
-
1986
- 1986-04-16 ES ES554045A patent/ES8800439A1/es not_active Expired
-
1987
- 1987-02-13 US US07/015,563 patent/US4889798A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0151492A2 (en) | 1985-08-14 |
| DK61085A (da) | 1985-08-10 |
| ES540250A0 (es) | 1988-04-01 |
| DE3588078D1 (de) | 1996-02-29 |
| ES554045A0 (es) | 1987-10-16 |
| IL74262A0 (en) | 1985-05-31 |
| US4889798A (en) | 1989-12-26 |
| ES8800439A1 (es) | 1987-10-16 |
| EP0151492A3 (en) | 1988-11-09 |
| EP0151492B1 (en) | 1996-01-17 |
| ATE133261T1 (de) | 1996-02-15 |
| DK61085D0 (da) | 1985-02-08 |
| JPS60188095A (ja) | 1985-09-25 |
| CA1314810C (en) | 1993-03-23 |
| DE3588078T2 (de) | 1996-09-19 |
| ES8802188A1 (es) | 1988-04-01 |
| AU3844485A (en) | 1985-08-15 |
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