GB2239948B - Fluorescence assay using phosphatase and a naphthol phosphate derivative - Google Patents

Fluorescence assay using phosphatase and a naphthol phosphate derivative

Info

Publication number
GB2239948B
GB2239948B GB9027423A GB9027423A GB2239948B GB 2239948 B GB2239948 B GB 2239948B GB 9027423 A GB9027423 A GB 9027423A GB 9027423 A GB9027423 A GB 9027423A GB 2239948 B GB2239948 B GB 2239948B
Authority
GB
United Kingdom
Prior art keywords
phosphatase
fluorescence assay
phosphate derivative
naphthol phosphate
naphthol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB9027423A
Other versions
GB2239948A (en
GB9027423D0 (en
Inventor
Satoshi Fujita
Naoto Kagiyama
Masayoshi Momiyama
Hiroshi Hori
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aisin Corp
Original Assignee
Aisin Seiki Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP23222390A external-priority patent/JPH03251200A/en
Application filed by Aisin Seiki Co Ltd filed Critical Aisin Seiki Co Ltd
Publication of GB9027423D0 publication Critical patent/GB9027423D0/en
Publication of GB2239948A publication Critical patent/GB2239948A/en
Application granted granted Critical
Publication of GB2239948B publication Critical patent/GB2239948B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C07F9/094—Esters of phosphoric acids with arylalkanols
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
GB9027423A 1989-12-28 1990-12-18 Fluorescence assay using phosphatase and a naphthol phosphate derivative Expired - Fee Related GB2239948B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP34315389 1989-12-28
JP23222390A JPH03251200A (en) 1989-12-28 1990-08-31 Determining nucleic acid or the like with naphthol as phosphoric acid

Publications (3)

Publication Number Publication Date
GB9027423D0 GB9027423D0 (en) 1991-02-06
GB2239948A GB2239948A (en) 1991-07-17
GB2239948B true GB2239948B (en) 1994-01-12

Family

ID=26530342

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9027423A Expired - Fee Related GB2239948B (en) 1989-12-28 1990-12-18 Fluorescence assay using phosphatase and a naphthol phosphate derivative

Country Status (2)

Country Link
DE (1) DE4041880C2 (en)
GB (1) GB2239948B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2646814B2 (en) * 1990-08-07 1997-08-27 アイシン精機株式会社 Assay method for nucleic acids, etc.
JPH04222600A (en) * 1990-12-21 1992-08-12 Aisin Seiki Co Ltd Detection of nucleic acid or the like by phosphate of naphthol derivative
JPH06197798A (en) * 1992-08-28 1994-07-19 Aisin Seiki Co Ltd Determination of dna base sequence by fluorescent substrate

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3844485A (en) * 1984-02-09 1985-08-15 Enzo Biochem Inc. Heterologous detection of cabeled dna

Also Published As

Publication number Publication date
GB2239948A (en) 1991-07-17
GB9027423D0 (en) 1991-02-06
DE4041880A1 (en) 1991-07-04
DE4041880C2 (en) 1994-03-10

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19971218