DK188184A - Fremgangsmaade til dannelse af et kompleks af biologisk aktive eller funktionelle forbindelser og anvendelse af forbindelserne - Google Patents

Fremgangsmaade til dannelse af et kompleks af biologisk aktive eller funktionelle forbindelser og anvendelse af forbindelserne Download PDF

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Publication number
DK188184A
DK188184A DK188184A DK188184A DK188184A DK 188184 A DK188184 A DK 188184A DK 188184 A DK188184 A DK 188184A DK 188184 A DK188184 A DK 188184A DK 188184 A DK188184 A DK 188184A
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compounds
complex
creating
procedure
biological active
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DK188184A
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DK188184D0 (da
Inventor
Kenneth H Johnston
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Enzo Biochem Inc
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Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc
Publication of DK188184D0 publication Critical patent/DK188184D0/da
Publication of DK188184A publication Critical patent/DK188184A/da

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • Inorganic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Description

en ligand, såsom en kompetitiv inhibitor for alkalisk phosphatase, f.eks. para-aminobenzyl-phosphonsyre (p-ABPA). Dextran-kuglerne ville først blive behandlet med (p-ABPA), og alkalisk phosphatase ville derefter blive bragt i kontakt med de således behandlede dextrankugler. Den kompetitive inhibitor p-ABPA virker som en ligand for fæstnelse af alkalisk phosphatase til dextrankuglerne, medens den på samme tid optager og beskytter det aktive sted af alkalisk phosphatase. Derefter derivatiseres eller modificeres den således fæstnede alkaliske phosphatase, f.eks. ved biotinylering. Efter biotinylering af den alkaliske phosphatase elueres den resulterende biotinylerede alkaliske phosphatase eller fjernes fra fæstnelse til det p-ABPA-behandlede faste substrat eller dextrankugler, hvortil det var bundet, til dannelse af en biotinyleret alkalisk phosphatase med det aktive eller funktionelle sted af denne intakt. Komplekset kunne, medens det stadig var bundet til dextrankuglerne, behandles yderligere med en anden forbindelse eller komponent, som også om ønsket kunne tilvejebringe et aktive eller funktionelt sted, f.eks. ved kontakt med avidin, der ville binde til den modificerende eller derivatiserende del, dvs. biotin, af den biotinylerede alkaliske phosphatase, og det således dannede avidin-biotiny-lerede, alkaliske phosphatasekompleks kunne derefter elueres. Dette kompleks, såsom et kompleks indbefattende avidin-bioti-nyleret alkalisk phosphatase kunne anvendes som en detektor i et detektionssystem til identifikationen og/eller lokaliseringen af en anden forbindelse, såsom en biotinyleret forbindelse, f.eks. et biotinyleret nucleotid eller biotinyleret DNA.
DK188184A 1983-04-20 1984-04-11 Fremgangsmaade til dannelse af et kompleks af biologisk aktive eller funktionelle forbindelser og anvendelse af forbindelserne DK188184A (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48692483A 1983-04-20 1983-04-20
US48692483 1983-04-20

Publications (2)

Publication Number Publication Date
DK188184D0 DK188184D0 (da) 1984-04-11
DK188184A true DK188184A (da) 1984-10-21

Family

ID=23933679

Family Applications (1)

Application Number Title Priority Date Filing Date
DK188184A DK188184A (da) 1983-04-20 1984-04-11 Fremgangsmaade til dannelse af et kompleks af biologisk aktive eller funktionelle forbindelser og anvendelse af forbindelserne

Country Status (7)

Country Link
EP (1) EP0123300A3 (da)
JP (1) JPS59224564A (da)
AU (1) AU2716184A (da)
DK (1) DK188184A (da)
ES (1) ES531734A0 (da)
IL (1) IL71577A0 (da)
NO (1) NO841580L (da)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61501047A (ja) * 1984-01-27 1986-05-22 モレキュラ−・バイオシステムズ，インコ−ポレイテッド 固定化したリポ−タ−グル−プ類の測定方法
AU3844485A (en) * 1984-02-09 1985-08-15 Enzo Biochem Inc. Heterologous detection of cabeled dna
US5093232A (en) * 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4937188A (en) * 1986-04-15 1990-06-26 Northeastern University Enzyme activity amplification method for increasing assay sensitivity
US5190864A (en) * 1986-04-15 1993-03-02 Northeastern University Enzyme amplification by using free enzyme to release enzyme from an immobilized enzyme material
US5047523A (en) * 1988-08-02 1991-09-10 Ortho Diagnostic Systems Inc. Nucleic acid probe for detection of neisseria gonorrhoea
US5099011A (en) * 1988-08-02 1992-03-24 Ortho Diagnostic Systems Inc. Nucleic acid probe for detection of Neisseria gonorrhoea
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
IT1238214B (it) * 1989-10-31 1993-07-12 Lorenzo Ferrari Formulazioni solide di sistemi complessi tipo sostanza avidinica-marcatore biotinilato utili in analisi biologiche.
BE1006179A3 (fr) * 1992-09-22 1994-05-31 Lambdatech S A Conjugue destine a la detection et/ou au dosage d'un compose biologique.
US5446137B1 (en) * 1993-12-09 1998-10-06 Behringwerke Ag Oligonucleotides containing 4'-substituted nucleotides
JP3781934B2 (ja) 1999-12-22 2006-06-07 株式会社ニチレイバイオサイエンス 酵素−タンパク質複合体
AU2006213692C1 (en) 2005-02-09 2011-08-04 Pacific Biosciences Of California, Inc. Nucleotide compositions and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4228237A (en) * 1978-09-21 1980-10-14 Calbiochem-Behring Corp. Methods for the detection and determination of ligands
US4371515A (en) * 1978-12-26 1983-02-01 E-Y Laboratories, Inc. Method for forming an isolated lectin-immunological conjugate
AU8594382A (en) * 1981-08-05 1983-02-10 F. Hoffmann-La Roche Ag Immunoassay using biotin-avidin-enzyme complex
US4617261A (en) * 1984-03-21 1986-10-14 Cetus Corporation Process for labeling nucleic acids and hybridization probes

Also Published As

Publication number Publication date
AU2716184A (en) 1984-10-25
ES8600393A1 (es) 1985-10-01
IL71577A0 (en) 1984-07-31
DK188184D0 (da) 1984-04-11
EP0123300A3 (en) 1988-01-20
NO841580L (no) 1984-10-22
ES531734A0 (es) 1985-10-01
JPS59224564A (ja) 1984-12-17
EP0123300A2 (en) 1984-10-31

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