WO2000052201A3 - Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung - Google Patents

Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung Download PDF

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Publication number
WO2000052201A3
WO2000052201A3 PCT/EP2000/001595 EP0001595W WO0052201A3 WO 2000052201 A3 WO2000052201 A3 WO 2000052201A3 EP 0001595 W EP0001595 W EP 0001595W WO 0052201 A3 WO0052201 A3 WO 0052201A3
Authority
WO
WIPO (PCT)
Prior art keywords
detecting
test system
splicing
splicing reaction
possibly
Prior art date
Application number
PCT/EP2000/001595
Other languages
English (en)
French (fr)
Other versions
WO2000052201A2 (de
Inventor
Christoph Huels
Bettina Bauer
Claus Simandi
Reinhard Luehrmann
Tilmann Achsel
Hans-Peter Vornlocher
Original Assignee
Aventis Res & Tech Gmbh & Co
Christoph Huels
Bettina Bauer
Claus Simandi
Reinhard Luehrmann
Tilmann Achsel
Vornlocher Hans Peter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Res & Tech Gmbh & Co, Christoph Huels, Bettina Bauer, Claus Simandi, Reinhard Luehrmann, Tilmann Achsel, Vornlocher Hans Peter filed Critical Aventis Res & Tech Gmbh & Co
Priority to AU29154/00A priority Critical patent/AU2915400A/en
Priority to HU0203058A priority patent/HUP0203058A2/hu
Priority to NZ513908A priority patent/NZ513908A/xx
Priority to EP00907635A priority patent/EP1159449A2/de
Priority to JP2000602811A priority patent/JP2002537822A/ja
Priority to US09/857,063 priority patent/US6579681B1/en
Priority to CA002363431A priority patent/CA2363431A1/en
Publication of WO2000052201A2 publication Critical patent/WO2000052201A2/de
Publication of WO2000052201A3 publication Critical patent/WO2000052201A3/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Testsystem enthaltend: (a) eine oder mehrere, gleiche oder verschiedene immobilisierte Nukleinsäure/n mit mindestens einer spleissfähigen Nukleinsäure, (b) mindestens ein gelfreies Nachweissystem zum Nachweis einer Spleissreaktion, gegebenenfalls, (c) mindestens eine Zusammensetzung enthaltend Spleisskomponenten, und vorzugsweise, (d) geeignete Nachweissonden, und gegebenenfalls, (e) weitere Hilfsmittel.
PCT/EP2000/001595 1999-03-02 2000-02-25 Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung WO2000052201A2 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU29154/00A AU2915400A (en) 1999-03-02 2000-02-25 Test system for detecting a splicing reaction and use thereof
HU0203058A HUP0203058A2 (hu) 1999-03-02 2000-02-25 Vizsgáló rendszer összefonódási reakció kimutatására, és ennek alkalmazása
NZ513908A NZ513908A (en) 1999-03-02 2000-02-25 A gel free detection system comprising at least one probe for detecting a splicing reaction
EP00907635A EP1159449A2 (de) 1999-03-02 2000-02-25 Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung
JP2000602811A JP2002537822A (ja) 1999-03-02 2000-02-25 スプライシング反応を検出するための試験系およびその使用
US09/857,063 US6579681B1 (en) 1999-03-02 2000-02-25 Test system for detecting a splicing reaction and use thereof
CA002363431A CA2363431A1 (en) 1999-03-02 2000-02-25 Test system for detecting a splicing reaction and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19909156.0 1999-03-02
DE19909156A DE19909156A1 (de) 1999-03-02 1999-03-02 Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung

Publications (2)

Publication Number Publication Date
WO2000052201A2 WO2000052201A2 (de) 2000-09-08
WO2000052201A3 true WO2000052201A3 (de) 2001-05-03

Family

ID=7899465

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/001595 WO2000052201A2 (de) 1999-03-02 2000-02-25 Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung

Country Status (10)

Country Link
US (1) US6579681B1 (de)
EP (1) EP1159449A2 (de)
JP (1) JP2002537822A (de)
AU (1) AU2915400A (de)
CA (1) CA2363431A1 (de)
DE (1) DE19909156A1 (de)
HU (1) HUP0203058A2 (de)
NZ (1) NZ513908A (de)
PL (1) PL355179A1 (de)
WO (1) WO2000052201A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10018465A1 (de) * 2000-04-14 2001-10-18 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung
DE10150783A1 (de) * 2001-10-15 2003-04-24 Bayer Cropscience Ag Splicing als Target zum Identifizieren neuer Wirkstoffe
US8822168B2 (en) * 2005-03-11 2014-09-02 The Trustees Of The University Of Pennsylvania Assays for detecting small nuclear ribonucleoprotein particle assembly and survival of motor neurons activity
DE102007031574B4 (de) 2007-07-06 2012-01-26 Karlsruher Institut für Technologie Minor-Spleißosom Testsystem zur Modulierung der Zellteilung

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995007364A1 (en) * 1993-09-08 1995-03-16 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands and improved methods for producing the same
WO1997015679A1 (en) * 1995-10-27 1997-05-01 The Trustees Of The University Of Pennsylvania Recombinant viruses containing mobile genetic elements and methods of use in gene therapy
WO1998008381A1 (en) * 1996-08-26 1998-03-05 University Of Washington Therapeutic and diagnostic applications of perlecan domain i splice variants

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849484A (en) * 1992-07-30 1998-12-15 University Of Medicine & Dentistry Of Nj In vitro assay for inhibitors of the intron self-splicing reaction in Pneumocystis carinii
EP0723407B1 (de) 1993-09-17 2000-08-23 Design/Analysis Consultants, Inc. Schirm

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995007364A1 (en) * 1993-09-08 1995-03-16 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands and improved methods for producing the same
WO1997015679A1 (en) * 1995-10-27 1997-05-01 The Trustees Of The University Of Pennsylvania Recombinant viruses containing mobile genetic elements and methods of use in gene therapy
WO1998008381A1 (en) * 1996-08-26 1998-03-05 University Of Washington Therapeutic and diagnostic applications of perlecan domain i splice variants

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL European Molecular Biology Laboratory; 1994, XP002155147 *
HUGHES J M X: "The yeast homologue of U3 snRNA", EMBO JOURNAL, vol. 6, no. 7, 1987, pages 2145 - 2155, XP002155146 *
JENISON R D ET AL: "HIGH-RESOLUTION MOLECULAR DISCRIMINATION BY RNA", SCIENCE,US,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, vol. 263, 11 March 1994 (1994-03-11), pages 1425 - 1429, XP000567866, ISSN: 0036-8075 *
KONARSKA M M ET AL.: "Characterization of the branch site in lariat RNAs produced by splicing of mRNA precursors", NATURE, vol. 313, 1985, pages 552 - 557, XP002155145 *
MORGAN W R AND WARD D C: "Three splicing patterns are used to excice the small intron common to all minute virus of mice RNAs", JOURNAL OF VIROLOGY, vol. 60, no. 3, 1986, pages 1170 - 1174, XP000961665 *
MOUGIN A ET AL.: "Secondary structure of the yeast Saccharmyces cerevisiae pre-U3A snoRNA and its implication for splicing efficiency", RNA, vol. 2, 1996, pages 1079 - 1093, XP002155144 *
SAWA H AND SHIMURA Y: "Alterations of RNase H sensitivity of the 3' splice site region during the in vitro splicing reaction", NUCLEIC ACIDS RESEARCH, vol. 19, no. 14, 1991, pages 3953 - 3958, XP002155141 *
UTANS U AND KRÄMER A: "Splicing factor SF4 is dispensable for the assembly of a functional splicing complex and participates in the subsequent steps of the splicing reaction", THE EMBO JOURNAL, vol. 9, no. 12, 1990, pages 4119 - 4126, XP002155143 *
WANG Y ET AL.: "RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities", BIOCHEMISTRY, vol. 35, 1996, pages 12338 - 12346, XP002155142 *

Also Published As

Publication number Publication date
AU2915400A (en) 2000-09-21
DE19909156A1 (de) 2000-09-07
WO2000052201A2 (de) 2000-09-08
NZ513908A (en) 2001-09-28
US6579681B1 (en) 2003-06-17
EP1159449A2 (de) 2001-12-05
HUP0203058A2 (hu) 2002-12-28
JP2002537822A (ja) 2002-11-12
CA2363431A1 (en) 2000-09-08
PL355179A1 (en) 2004-04-05

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