ES8600393A1 - Un metodo de detectar un nucleotido marcado quimicamente. - Google Patents

Un metodo de detectar un nucleotido marcado quimicamente.

Info

Publication number
ES8600393A1
ES8600393A1 ES531734A ES531734A ES8600393A1 ES 8600393 A1 ES8600393 A1 ES 8600393A1 ES 531734 A ES531734 A ES 531734A ES 531734 A ES531734 A ES 531734A ES 8600393 A1 ES8600393 A1 ES 8600393A1
Authority
ES
Spain
Prior art keywords
compound
alkaline phosphatase
biotinylated
complex
solid substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES531734A
Other languages
English (en)
Other versions
ES531734A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzo Biochem Inc
Original Assignee
Enzo Biochem Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc
Publication of ES531734A0 publication Critical patent/ES531734A0/es
Publication of ES8600393A1 publication Critical patent/ES8600393A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)

Abstract

METODO PARA LA MODIFICACION DE UNA ENZIMA BIOLOGICAMENTE ACTIVA.COMPRENDE: PONER EN CONTACTO LA ENZIMA CON UN SUBSTRATO SOLIDO QUE COMPRENDE UN COMPUESTO INMOVILIZADO SOBRE EL Y QUE SE FIJA A UN SITIO ACTIVO DE LA ENZIMA, CON LO QUE LA ENZIMA ES INMOVILIZADA SOBRE EL SUBSTRATO A TRAVES DE SU SITIO ACTIVO; COLOCAR LA ENZIMA INMOVILIZADA SOBRE UN MARCADOR ACTIVADO QUE UNE LA ENZIMA EN UN SEGUNDO SITIO DISTINTO DEL SITIO ACTIVO, POR LO QUE SE FORMA UNA ENTIDAD INMOVILIZADA EN EL SEGUNDO SITIO; Y ELUIR A PARTIR DE LA ASOCIACION DE ENZIMA-MARCADOR QUE TIENE UN SITIO ACTIVO DE ENZIMA LIBRE SELECCIONADO.
ES531734A 1983-04-20 1984-04-18 Un metodo de detectar un nucleotido marcado quimicamente. Expired ES8600393A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US48692483A 1983-04-20 1983-04-20

Publications (2)

Publication Number Publication Date
ES531734A0 ES531734A0 (es) 1985-10-01
ES8600393A1 true ES8600393A1 (es) 1985-10-01

Family

ID=23933679

Family Applications (1)

Application Number Title Priority Date Filing Date
ES531734A Expired ES8600393A1 (es) 1983-04-20 1984-04-18 Un metodo de detectar un nucleotido marcado quimicamente.

Country Status (7)

Country Link
EP (1) EP0123300A3 (es)
JP (1) JPS59224564A (es)
AU (1) AU2716184A (es)
DK (1) DK188184A (es)
ES (1) ES8600393A1 (es)
IL (1) IL71577A0 (es)
NO (1) NO841580L (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985003356A1 (en) * 1984-01-27 1985-08-01 Molecular Biosystems, Inc. Assay for immobilized reporter groups
AU3844485A (en) * 1984-02-09 1985-08-15 Enzo Biochem Inc. Heterologous detection of cabeled dna
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US5093232A (en) * 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4937188A (en) * 1986-04-15 1990-06-26 Northeastern University Enzyme activity amplification method for increasing assay sensitivity
US5190864A (en) * 1986-04-15 1993-03-02 Northeastern University Enzyme amplification by using free enzyme to release enzyme from an immobilized enzyme material
US5099011A (en) * 1988-08-02 1992-03-24 Ortho Diagnostic Systems Inc. Nucleic acid probe for detection of Neisseria gonorrhoea
US5047523A (en) * 1988-08-02 1991-09-10 Ortho Diagnostic Systems Inc. Nucleic acid probe for detection of neisseria gonorrhoea
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
IT1238214B (it) * 1989-10-31 1993-07-12 Lorenzo Ferrari Formulazioni solide di sistemi complessi tipo sostanza avidinica-marcatore biotinilato utili in analisi biologiche.
BE1006179A3 (fr) * 1992-09-22 1994-05-31 Lambdatech S A Conjugue destine a la detection et/ou au dosage d'un compose biologique.
US5446137B1 (en) * 1993-12-09 1998-10-06 Behringwerke Ag Oligonucleotides containing 4'-substituted nucleotides
JP3781934B2 (ja) * 1999-12-22 2006-06-07 株式会社ニチレイバイオサイエンス 酵素−タンパク質複合体
US7767394B2 (en) 2005-02-09 2010-08-03 Pacific Biosciences Of California, Inc. Nucleotide compositions and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4228237A (en) * 1978-09-21 1980-10-14 Calbiochem-Behring Corp. Methods for the detection and determination of ligands
US4371515A (en) * 1978-12-26 1983-02-01 E-Y Laboratories, Inc. Method for forming an isolated lectin-immunological conjugate
AU8594382A (en) * 1981-08-05 1983-02-10 F. Hoffmann-La Roche Ag Immunoassay using biotin-avidin-enzyme complex
US4617261A (en) * 1984-03-21 1986-10-14 Cetus Corporation Process for labeling nucleic acids and hybridization probes

Also Published As

Publication number Publication date
EP0123300A2 (en) 1984-10-31
ES531734A0 (es) 1985-10-01
JPS59224564A (ja) 1984-12-17
DK188184D0 (da) 1984-04-11
DK188184A (da) 1984-10-21
EP0123300A3 (en) 1988-01-20
NO841580L (no) 1984-10-22
IL71577A0 (en) 1984-07-31
AU2716184A (en) 1984-10-25

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