NO325588B1 - Immortaliserte cellelinjer, anvendelse av slike, fremgangsmate for immortalisering av celler samt kunstig hud omfattende nevnte immortaliserte cellelinje - Google Patents
Immortaliserte cellelinjer, anvendelse av slike, fremgangsmate for immortalisering av celler samt kunstig hud omfattende nevnte immortaliserte cellelinje Download PDFInfo
- Publication number
- NO325588B1 NO325588B1 NO20005053A NO20005053A NO325588B1 NO 325588 B1 NO325588 B1 NO 325588B1 NO 20005053 A NO20005053 A NO 20005053A NO 20005053 A NO20005053 A NO 20005053A NO 325588 B1 NO325588 B1 NO 325588B1
- Authority
- NO
- Norway
- Prior art keywords
- keratinocytes
- medium
- cells
- culture
- immortalized
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 18
- 210000002510 keratinocyte Anatomy 0.000 claims abstract description 120
- 210000004027 cell Anatomy 0.000 claims abstract description 106
- 239000002609 medium Substances 0.000 claims abstract description 63
- 210000003491 skin Anatomy 0.000 claims abstract description 33
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 28
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 17
- 230000004069 differentiation Effects 0.000 claims abstract description 14
- 210000000981 epithelium Anatomy 0.000 claims abstract description 13
- 230000035755 proliferation Effects 0.000 claims abstract description 13
- 210000002966 serum Anatomy 0.000 claims abstract description 12
- 210000004927 skin cell Anatomy 0.000 claims abstract description 12
- 210000000434 stratum corneum Anatomy 0.000 claims abstract description 12
- 238000000338 in vitro Methods 0.000 claims abstract description 10
- 239000011248 coating agent Substances 0.000 claims abstract description 9
- 238000000576 coating method Methods 0.000 claims abstract description 9
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 9
- 230000001177 retroviral effect Effects 0.000 claims abstract description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 8
- 239000011575 calcium Substances 0.000 claims abstract description 8
- 230000010261 cell growth Effects 0.000 claims abstract description 7
- 102000012422 Collagen Type I Human genes 0.000 claims abstract description 6
- 108010022452 Collagen Type I Proteins 0.000 claims abstract description 6
- 102000004190 Enzymes Human genes 0.000 claims abstract description 6
- 108090000790 Enzymes Proteins 0.000 claims abstract description 6
- 102000016359 Fibronectins Human genes 0.000 claims abstract description 6
- 108010067306 Fibronectins Proteins 0.000 claims abstract description 6
- 229940096422 collagen type i Drugs 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 6
- 230000001900 immune effect Effects 0.000 claims abstract description 6
- 230000000144 pharmacologic effect Effects 0.000 claims abstract description 6
- 206010040914 Skin reaction Diseases 0.000 claims abstract description 5
- 230000035483 skin reaction Effects 0.000 claims abstract description 5
- 231100000430 skin reaction Toxicity 0.000 claims abstract description 5
- 238000010899 nucleation Methods 0.000 claims abstract description 4
- 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 claims description 28
- 210000001519 tissue Anatomy 0.000 claims description 18
- 239000013598 vector Substances 0.000 claims description 13
- 241000341655 Human papillomavirus type 16 Species 0.000 claims description 10
- 230000012010 growth Effects 0.000 claims description 9
- 230000001939 inductive effect Effects 0.000 claims description 6
- 101000693922 Bos taurus Albumin Proteins 0.000 claims description 5
- 241001631646 Papillomaviridae Species 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 101150013359 E7 gene Proteins 0.000 claims description 3
- 101150071673 E6 gene Proteins 0.000 claims description 2
- XDMCWZFLLGVIID-SXPRBRBTSA-N O-(3-O-D-galactosyl-N-acetyl-beta-D-galactosaminyl)-L-serine Chemical compound CC(=O)N[C@H]1[C@H](OC[C@H]([NH3+])C([O-])=O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 XDMCWZFLLGVIID-SXPRBRBTSA-N 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims description 2
- 230000008488 polyadenylation Effects 0.000 claims description 2
- 230000010076 replication Effects 0.000 claims description 2
- 108091081062 Repeated sequence (DNA) Proteins 0.000 claims 1
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 3
- 238000010276 construction Methods 0.000 abstract description 3
- 231100001221 nontumorigenic Toxicity 0.000 abstract description 3
- 101100148654 Arabidopsis thaliana SAB gene Proteins 0.000 abstract 1
- 102100040119 SH3 domain-binding protein 5 Human genes 0.000 abstract 1
- 241001430294 unidentified retrovirus Species 0.000 abstract 1
- 210000002752 melanocyte Anatomy 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
- 102000004142 Trypsin Human genes 0.000 description 7
- 108090000631 Trypsin Proteins 0.000 description 7
- 241000700605 Viruses Species 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000012588 trypsin Substances 0.000 description 7
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 description 6
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 6
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 6
- 210000002950 fibroblast Anatomy 0.000 description 6
- 239000012679 serum free medium Substances 0.000 description 6
- 241000701806 Human papillomavirus Species 0.000 description 5
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 229930003268 Vitamin C Natural products 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 235000019154 vitamin C Nutrition 0.000 description 4
- 239000011718 vitamin C Substances 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- PHEDXBVPIONUQT-UHFFFAOYSA-N Cocarcinogen A1 Natural products CCCCCCCCCCCCCC(=O)OC1C(C)C2(O)C3C=C(C)C(=O)C3(O)CC(CO)=CC2C2C1(OC(C)=O)C2(C)C PHEDXBVPIONUQT-UHFFFAOYSA-N 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 102000005720 Glutathione transferase Human genes 0.000 description 3
- 108010070675 Glutathione transferase Proteins 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 3
- 108700020796 Oncogene Proteins 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 210000004207 dermis Anatomy 0.000 description 3
- 239000012737 fresh medium Substances 0.000 description 3
- 210000005260 human cell Anatomy 0.000 description 3
- 230000016507 interphase Effects 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 210000004940 nucleus Anatomy 0.000 description 3
- PHEDXBVPIONUQT-RGYGYFBISA-N phorbol 13-acetate 12-myristate Chemical compound C([C@]1(O)C(=O)C(C)=C[C@H]1[C@@]1(O)[C@H](C)[C@H]2OC(=O)CCCCCCCCCCCCC)C(CO)=C[C@H]1[C@H]1[C@]2(OC(C)=O)C1(C)C PHEDXBVPIONUQT-RGYGYFBISA-N 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 102000029816 Collagenase Human genes 0.000 description 2
- 108060005980 Collagenase Proteins 0.000 description 2
- 102100028314 Filaggrin Human genes 0.000 description 2
- 101710088660 Filaggrin Proteins 0.000 description 2
- 102100040445 Keratin, type I cytoskeletal 14 Human genes 0.000 description 2
- 101710183391 Keratin, type I cytoskeletal 14 Proteins 0.000 description 2
- 102000011782 Keratins Human genes 0.000 description 2
- 108010076876 Keratins Proteins 0.000 description 2
- 101710128836 Large T antigen Proteins 0.000 description 2
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 2
- 102100031784 Loricrin Human genes 0.000 description 2
- 241000699660 Mus musculus Species 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 206010040880 Skin irritation Diseases 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 229960002424 collagenase Drugs 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 235000004626 essential fatty acids Nutrition 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000009931 harmful effect Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 102000007236 involucrin Human genes 0.000 description 2
- 108010033564 involucrin Proteins 0.000 description 2
- 235000020778 linoleic acid Nutrition 0.000 description 2
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 2
- 108010079309 loricrin Proteins 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 230000037311 normal skin Effects 0.000 description 2
- 238000011580 nude mouse model Methods 0.000 description 2
- 230000036556 skin irritation Effects 0.000 description 2
- 231100000475 skin irritation Toxicity 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- FMMWHPNWAFZXNH-UHFFFAOYSA-N Benz[a]pyrene Chemical compound C1=C2C3=CC=CC=C3C=C(C=C3)C2=C2C3=CC=CC2=C1 FMMWHPNWAFZXNH-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 108020005124 DNA Adducts Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 239000012983 Dulbecco’s minimal essential medium Substances 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 206010064912 Malignant transformation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101100338009 Mus musculus Gsta1 gene Proteins 0.000 description 1
- 101100123101 Mus musculus Gsta4 gene Proteins 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 201000004681 Psoriasis Diseases 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 241000714474 Rous sarcoma virus Species 0.000 description 1
- 101710162629 Trypsin inhibitor Proteins 0.000 description 1
- 229940122618 Trypsin inhibitor Drugs 0.000 description 1
- 102000013127 Vimentin Human genes 0.000 description 1
- 108010065472 Vimentin Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- -1 benzyl ester Chemical class 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- GEHJBWKLJVFKPS-UHFFFAOYSA-N bromochloroacetic acid Chemical compound OC(=O)C(Cl)Br GEHJBWKLJVFKPS-UHFFFAOYSA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000005188 flotation Methods 0.000 description 1
- 231100000025 genetic toxicology Toxicity 0.000 description 1
- 230000001738 genotoxic effect Effects 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 230000003780 keratinization Effects 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000036212 malign transformation Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000150 mutagenicity / genotoxicity testing Toxicity 0.000 description 1
- 230000009826 neoplastic cell growth Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000004633 phorbol derivatives Chemical class 0.000 description 1
- 239000002644 phorbol ester Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000003711 photoprotective effect Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 231100000051 skin sensitiser Toxicity 0.000 description 1
- 210000000438 stratum basale Anatomy 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 238000011477 surgical intervention Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- JOPDZQBPOWAEHC-UHFFFAOYSA-H tristrontium;diphosphate Chemical compound [Sr+2].[Sr+2].[Sr+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O JOPDZQBPOWAEHC-UHFFFAOYSA-H 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 231100000588 tumorigenic Toxicity 0.000 description 1
- 230000000381 tumorigenic effect Effects 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 210000005048 vimentin Anatomy 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 239000002676 xenobiotic agent Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0629—Keratinocytes; Whole skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
- C12N2500/14—Calcium; Ca chelators; Calcitonin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/04—Screening or testing on artificial tissues
- C12N2503/06—Screening or testing on artificial skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Prostheses (AREA)
- Woven Fabrics (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98201247 | 1998-04-17 | ||
| PCT/EP1999/002347 WO1999054435A2 (fr) | 1998-04-17 | 1999-04-07 | Lignee de cellules immortalisees derivees de tissus cutanes humains normaux |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO20005053D0 NO20005053D0 (no) | 2000-10-06 |
| NO20005053L NO20005053L (no) | 2000-11-29 |
| NO325588B1 true NO325588B1 (no) | 2008-06-23 |
Family
ID=8233616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO20005053A NO325588B1 (no) | 1998-04-17 | 2000-10-06 | Immortaliserte cellelinjer, anvendelse av slike, fremgangsmate for immortalisering av celler samt kunstig hud omfattende nevnte immortaliserte cellelinje |
Country Status (22)
| Country | Link |
|---|---|
| US (1) | US6566136B2 (cs) |
| EP (1) | EP1071747B1 (cs) |
| JP (1) | JP4671504B2 (cs) |
| KR (1) | KR20010074488A (cs) |
| CN (1) | CN1299409A (cs) |
| AT (1) | ATE274050T1 (cs) |
| AU (1) | AU756151B2 (cs) |
| BR (1) | BR9909699A (cs) |
| CA (1) | CA2324479C (cs) |
| CZ (1) | CZ20003842A3 (cs) |
| DE (1) | DE69919531T2 (cs) |
| DK (1) | DK1071747T3 (cs) |
| ES (1) | ES2226383T3 (cs) |
| HU (1) | HUP0101913A3 (cs) |
| IL (1) | IL138417A0 (cs) |
| NO (1) | NO325588B1 (cs) |
| NZ (1) | NZ506944A (cs) |
| PL (1) | PL201401B1 (cs) |
| PT (1) | PT1071747E (cs) |
| RU (1) | RU2244005C2 (cs) |
| SK (1) | SK286870B6 (cs) |
| WO (1) | WO1999054435A2 (cs) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6368815B1 (en) * | 1999-03-29 | 2002-04-09 | Warner-Lambert Company | Screening of molecules that inhibit human phosphodiesterase 4A produced by non-recombinant cell lines |
| EP1130086A1 (en) * | 2000-02-18 | 2001-09-05 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Genetically engineered keratinocytes and toxicity assay using said keratinocytes |
| IT1316995B1 (it) * | 2000-02-25 | 2003-05-26 | Idi Irccs | Procedimento per ottenere da colture primarie di cheratinociti umanilinee cellulari immortalizzate. |
| AU2002342613A1 (en) | 2001-05-09 | 2002-11-25 | Geron Corporation | Treatment for wounds |
| DE10255285A1 (de) * | 2002-11-26 | 2004-06-03 | Mcs Micro Carrier Systems Gmbh | Selbst formende Phospholipid-Gele |
| US8669109B2 (en) | 2003-08-19 | 2014-03-11 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Methods of producing proteins in Chinese hamster ovary (CHO) cells |
| DE10338531A1 (de) | 2003-08-19 | 2005-04-07 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Verfahren zur Reklonierung von Produktionszellen |
| EP1557085A1 (en) | 2004-01-21 | 2005-07-27 | Boehringer Ingelheim International GmbH | Mouse model for human psoriasis |
| WO2006047426A2 (en) * | 2004-10-22 | 2006-05-04 | The Regents Of The University Of Michigan | Compositions, methods and systems for preparation of a stem cell-enriched cell population |
| KR101323459B1 (ko) * | 2004-12-23 | 2013-10-29 | 메디뮨 엘엘씨 | 바이러스의 증식을 위한 비종양형성성 ｍｄｃｋ 세포주 |
| EP1739179A1 (en) * | 2005-06-30 | 2007-01-03 | Octapharma AG | Serum-free stable transfection and production of recombinant human proteins in human cell lines |
| CN105219697A (zh) * | 2014-07-04 | 2016-01-06 | 赫柏慧康生物科技无锡有限公司 | 一种人原代角质细胞分离制备的方法 |
| RU2573940C1 (ru) * | 2014-12-11 | 2016-01-27 | Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук | Клеточная линия рака почки человека ibgvat r 6 |
| RU2573938C1 (ru) * | 2014-12-11 | 2016-01-27 | Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук | Клеточная линия рака почки человека ibgvat r 27 |
| WO2023123299A1 (en) * | 2021-12-31 | 2023-07-06 | Beijing Theraxyte Bioscience Co., Ltd. | Compositions and methods for culturing stem cells |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2203630A1 (en) * | 1994-11-08 | 1996-05-17 | Bradley Michael John Stringer | Human cell-lines |
| CZ297289B6 (cs) * | 1995-12-21 | 2006-10-11 | Societe Des Produits Nestle S. A. | Imortalizované bunecné linie a zivné médium |
| US5811297A (en) * | 1996-03-07 | 1998-09-22 | Amba Biosciences, Llc | Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages |
-
1999
- 1999-04-07 DK DK99920605T patent/DK1071747T3/da active
- 1999-04-07 ES ES99920605T patent/ES2226383T3/es not_active Expired - Lifetime
- 1999-04-07 BR BR9909699-4A patent/BR9909699A/pt not_active Application Discontinuation
- 1999-04-07 RU RU2000129131/13A patent/RU2244005C2/ru not_active IP Right Cessation
- 1999-04-07 AT AT99920605T patent/ATE274050T1/de not_active IP Right Cessation
- 1999-04-07 IL IL13841799A patent/IL138417A0/xx unknown
- 1999-04-07 SK SK1549-2000A patent/SK286870B6/sk not_active IP Right Cessation
- 1999-04-07 CN CN99805127A patent/CN1299409A/zh active Pending
- 1999-04-07 HU HU0101913A patent/HUP0101913A3/hu unknown
- 1999-04-07 PT PT99920605T patent/PT1071747E/pt unknown
- 1999-04-07 EP EP99920605A patent/EP1071747B1/fr not_active Expired - Lifetime
- 1999-04-07 CZ CZ20003842A patent/CZ20003842A3/cs unknown
- 1999-04-07 WO PCT/EP1999/002347 patent/WO1999054435A2/fr active IP Right Grant
- 1999-04-07 PL PL364980A patent/PL201401B1/pl not_active IP Right Cessation
- 1999-04-07 CA CA002324479A patent/CA2324479C/en not_active Expired - Fee Related
- 1999-04-07 NZ NZ506944A patent/NZ506944A/en unknown
- 1999-04-07 DE DE69919531T patent/DE69919531T2/de not_active Expired - Lifetime
- 1999-04-07 KR KR1020007011556A patent/KR20010074488A/ko active IP Right Grant
- 1999-04-07 JP JP2000544768A patent/JP4671504B2/ja not_active Expired - Fee Related
- 1999-04-07 AU AU38135/99A patent/AU756151B2/en not_active Ceased
-
2000
- 2000-09-18 US US09/663,645 patent/US6566136B2/en not_active Expired - Lifetime
- 2000-10-06 NO NO20005053A patent/NO325588B1/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| SK15492000A3 (sk) | 2001-03-12 |
| CA2324479A1 (en) | 1999-10-28 |
| RU2244005C2 (ru) | 2005-01-10 |
| PL364980A1 (en) | 2004-12-27 |
| CA2324479C (en) | 2007-12-04 |
| CN1299409A (zh) | 2001-06-13 |
| CZ20003842A3 (cs) | 2001-08-15 |
| US20020042133A1 (en) | 2002-04-11 |
| ATE274050T1 (de) | 2004-09-15 |
| KR20010074488A (ko) | 2001-08-04 |
| PL201401B1 (pl) | 2009-04-30 |
| AU3813599A (en) | 1999-11-08 |
| NO20005053L (no) | 2000-11-29 |
| BR9909699A (pt) | 2002-04-30 |
| ES2226383T3 (es) | 2005-03-16 |
| DE69919531T2 (de) | 2005-09-15 |
| DE69919531D1 (de) | 2004-09-23 |
| NO20005053D0 (no) | 2000-10-06 |
| NZ506944A (en) | 2003-10-31 |
| EP1071747A2 (fr) | 2001-01-31 |
| JP2003516711A (ja) | 2003-05-20 |
| HUP0101913A2 (hu) | 2001-09-28 |
| PT1071747E (pt) | 2004-12-31 |
| IL138417A0 (en) | 2001-10-31 |
| WO1999054435A3 (fr) | 1999-12-09 |
| EP1071747B1 (fr) | 2004-08-18 |
| HUP0101913A3 (en) | 2002-06-28 |
| JP4671504B2 (ja) | 2011-04-20 |
| SK286870B6 (sk) | 2009-06-05 |
| WO1999054435A2 (fr) | 1999-10-28 |
| US6566136B2 (en) | 2003-05-20 |
| AU756151B2 (en) | 2003-01-02 |
| DK1071747T3 (da) | 2004-11-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100455006B1 (ko) | 향상된불사화사람피부세포계및이의제조에유용한신규한무혈청배지 | |
| NO325588B1 (no) | Immortaliserte cellelinjer, anvendelse av slike, fremgangsmate for immortalisering av celler samt kunstig hud omfattende nevnte immortaliserte cellelinje | |
| EP1303589B1 (en) | Pre-adipose cell lines | |
| JP2000506374A5 (cs) | ||
| Grossi et al. | Generation of knockout human primary keratinocytes by CRISPR/Cas9 | |
| MXPA00009558A (en) | Immortalised cell lines derived from normal human skin tissues | |
| Hamblin et al. | Isolation and culture of vascular smooth muscle cells | |
| Oiticica et al. | Organ of Corti culture for functional analysis of precursor, support and hair cells | |
| Ramalho et al. | CULTURA DO ÓRGÃO DE CORTI IN VITRO PARA ANÁLISE FUNCIONAL DE CÉLULAS PRECURSORAS, DE SUPORTE E CILIADAS.. IN VITRO CULTURING OF ORGAN OF CORTI FOR FUNCTIONAL ANALYSIS OF PRECURSOR, SUPPORT AND HAIR CELLS.. | |
| MD et al. | Organ of Corti Culture for Functional Analysis of Precursor, Support and Hair Cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM1K | Lapsed by not paying the annual fees |