BR9909699A - Linhagem de células imortalizadas derivadas de tecidos cutâneos humanos normais - Google Patents

Linhagem de células imortalizadas derivadas de tecidos cutâneos humanos normais

Info

Publication number
BR9909699A
BR9909699A BR9909699-4A BR9909699A BR9909699A BR 9909699 A BR9909699 A BR 9909699A BR 9909699 A BR9909699 A BR 9909699A BR 9909699 A BR9909699 A BR 9909699A
Authority
BR
Brazil
Prior art keywords
keratinocytes
immortalized
cell line
normal human
fact
Prior art date
Application number
BR9909699-4A
Other languages
English (en)
Inventor
Markus Baur
Original Assignee
Nestle Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nestle Sa filed Critical Nestle Sa
Publication of BR9909699A publication Critical patent/BR9909699A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues
    • C12N2503/06—Screening or testing on artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Prostheses (AREA)
  • Woven Fabrics (AREA)

Abstract

Patente de invenção: "LINHAGEM DE CéLULAS IMORTALIZADAS DERIVADAS DE TECIDOS CUTâNEOS HUMANOS NORMAIS". Linhagem celular de queratinócitos humanos imortalizados por meio de pelo menos um gene tumorígeno funcional proveniente de um retrovírus caracterizada pelo fato de que ela: (1) é não tumorigênica; (2) conserva a aptidão à diferenciação e à expressão de proteínas e de enzimas que são expressas pelos queratinócitos diferenciados normais mesmo depois de uma taxa elevada de passagens em cultura de tecidos; e (3) forma um epitélio estratificado e polarizado que possui um stratum corneum orto-queratósico, quando ela é cultivada em cultura organotípica em um meio sem soro e sem camada de células nutrientes. Processo aperfeiçoado para imortalizar células cutâneas humanas a fim de obter queratinócitos imortalizados, caracterizado pelo fato de que ele compreende a etapa de infecção dos queratinócitos utilizando-se para isso genes tumorígenos funcionais de pelo menos dois retrovírus diferentes. Utilização dos queratinócitos de acordo com a invenção para análises imunológicas, farmacológicas, foto e quimiotoxicológicas de reação cutânea, para a expressão de genes heterólogos, e para a construção de uma pele artificial.
BR9909699-4A 1998-04-17 1999-04-07 Linhagem de células imortalizadas derivadas de tecidos cutâneos humanos normais BR9909699A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98201247 1998-04-17
PCT/EP1999/002347 WO1999054435A2 (fr) 1998-04-17 1999-04-07 Lignee de cellules immortalisees derivees de tissus cutanes humains normaux

Publications (1)

Publication Number Publication Date
BR9909699A true BR9909699A (pt) 2002-04-30

Family

ID=8233616

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9909699-4A BR9909699A (pt) 1998-04-17 1999-04-07 Linhagem de células imortalizadas derivadas de tecidos cutâneos humanos normais

Country Status (22)

Country Link
US (1) US6566136B2 (pt)
EP (1) EP1071747B1 (pt)
JP (1) JP4671504B2 (pt)
KR (1) KR20010074488A (pt)
CN (1) CN1299409A (pt)
AT (1) ATE274050T1 (pt)
AU (1) AU756151B2 (pt)
BR (1) BR9909699A (pt)
CA (1) CA2324479C (pt)
CZ (1) CZ20003842A3 (pt)
DE (1) DE69919531T2 (pt)
DK (1) DK1071747T3 (pt)
ES (1) ES2226383T3 (pt)
HU (1) HUP0101913A3 (pt)
IL (1) IL138417A0 (pt)
NO (1) NO325588B1 (pt)
NZ (1) NZ506944A (pt)
PL (1) PL201401B1 (pt)
PT (1) PT1071747E (pt)
RU (1) RU2244005C2 (pt)
SK (1) SK286870B6 (pt)
WO (1) WO1999054435A2 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6368815B1 (en) * 1999-03-29 2002-04-09 Warner-Lambert Company Screening of molecules that inhibit human phosphodiesterase 4A produced by non-recombinant cell lines
EP1130086A1 (en) * 2000-02-18 2001-09-05 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Genetically engineered keratinocytes and toxicity assay using said keratinocytes
IT1316995B1 (it) * 2000-02-25 2003-05-26 Idi Irccs Procedimento per ottenere da colture primarie di cheratinociti umanilinee cellulari immortalizzate.
AU2002342613A1 (en) 2001-05-09 2002-11-25 Geron Corporation Treatment for wounds
DE10255285A1 (de) * 2002-11-26 2004-06-03 Mcs Micro Carrier Systems Gmbh Selbst formende Phospholipid-Gele
US8669109B2 (en) 2003-08-19 2014-03-11 Boehringer Ingelheim Pharma Gmbh & Co. Kg Methods of producing proteins in Chinese hamster ovary (CHO) cells
DE10338531A1 (de) 2003-08-19 2005-04-07 Boehringer Ingelheim Pharma Gmbh & Co. Kg Verfahren zur Reklonierung von Produktionszellen
EP1557085A1 (en) 2004-01-21 2005-07-27 Boehringer Ingelheim International GmbH Mouse model for human psoriasis
WO2006047426A2 (en) * 2004-10-22 2006-05-04 The Regents Of The University Of Michigan Compositions, methods and systems for preparation of a stem cell-enriched cell population
KR101323459B1 (ko) * 2004-12-23 2013-10-29 메디뮨 엘엘씨 바이러스의 증식을 위한 비종양형성성 ｍｄｃｋ 세포주
EP1739179A1 (en) * 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
CN105219697A (zh) * 2014-07-04 2016-01-06 赫柏慧康生物科技无锡有限公司 一种人原代角质细胞分离制备的方法
RU2573940C1 (ru) * 2014-12-11 2016-01-27 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Клеточная линия рака почки человека ibgvat r 6
RU2573938C1 (ru) * 2014-12-11 2016-01-27 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Клеточная линия рака почки человека ibgvat r 27
WO2023123299A1 (en) * 2021-12-31 2023-07-06 Beijing Theraxyte Bioscience Co., Ltd. Compositions and methods for culturing stem cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2203630A1 (en) * 1994-11-08 1996-05-17 Bradley Michael John Stringer Human cell-lines
CZ297289B6 (cs) * 1995-12-21 2006-10-11 Societe Des Produits Nestle S. A. Imortalizované bunecné linie a zivné médium
US5811297A (en) * 1996-03-07 1998-09-22 Amba Biosciences, Llc Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages

Also Published As

Publication number Publication date
SK15492000A3 (sk) 2001-03-12
CA2324479A1 (en) 1999-10-28
RU2244005C2 (ru) 2005-01-10
PL364980A1 (en) 2004-12-27
CA2324479C (en) 2007-12-04
CN1299409A (zh) 2001-06-13
CZ20003842A3 (cs) 2001-08-15
US20020042133A1 (en) 2002-04-11
ATE274050T1 (de) 2004-09-15
KR20010074488A (ko) 2001-08-04
PL201401B1 (pl) 2009-04-30
AU3813599A (en) 1999-11-08
NO20005053L (no) 2000-11-29
ES2226383T3 (es) 2005-03-16
DE69919531T2 (de) 2005-09-15
DE69919531D1 (de) 2004-09-23
NO20005053D0 (no) 2000-10-06
NZ506944A (en) 2003-10-31
EP1071747A2 (fr) 2001-01-31
JP2003516711A (ja) 2003-05-20
HUP0101913A2 (hu) 2001-09-28
PT1071747E (pt) 2004-12-31
NO325588B1 (no) 2008-06-23
IL138417A0 (en) 2001-10-31
WO1999054435A3 (fr) 1999-12-09
EP1071747B1 (fr) 2004-08-18
HUP0101913A3 (en) 2002-06-28
JP4671504B2 (ja) 2011-04-20
SK286870B6 (sk) 2009-06-05
WO1999054435A2 (fr) 1999-10-28
US6566136B2 (en) 2003-05-20
AU756151B2 (en) 2003-01-02
DK1071747T3 (da) 2004-11-29

Similar Documents

Publication Publication Date Title
BR9909699A (pt) Linhagem de células imortalizadas derivadas de tecidos cutâneos humanos normais
Boukamp et al. Mesenchyme-mediated and endogenous regulation of growth and differentiation of human skin keratinocytes derived from different body sites
MATSUMOTO A study of an in vitro model for invasion of oral squamous cell carcinoma
Kusukawa et al. Expression of matrix metalloproteinase-2 related to lymph node metastasis of oral squamous cell carcinoma: a clinicopathologic study
Reznikoff et al. Growth and characterization of normal human urothelium in vitro
Farquhar et al. Functional organization of amphibian skin
Scott et al. Localization of basic fibroblast growth factor mRNA in melanocytic lesions by in situ hybridization
Fan et al. Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane
Chen et al. Type VII collagen gene expression by human skin fibroblasts and keratinocytes in culture: influence of donor age and cytokine responses
ATE262358T1 (de) Zusammensetzung zur gewebsregeneration
US20010048917A1 (en) Skin equivalent and methods of forming and using same
WO2006029347A3 (en) Integrin cd18 is a novel stromal stem cell marker and functions to promote osteogenesis
Seline et al. Expression of E and P-cadherin by melanoma cells decreases in progressive melanomas and following ultraviolet radiation
BR9811675A (pt) Genes isolados para proteìna da membrana de célula dentrìtica
Fusenig et al. Differentiation and tumor progression
Mahieu et al. The expression of carbonic anhydrases II and IV in the human pancreatic cancer cell line (Capan 1) is associated with bicarbonate ion channels
JP2003516711A5 (pt)
DE60038630D1 (de) Verfahren zur ex vivo bildung von säugetier-knochen und dessen verwendungen
Wyllie et al. Human thyroid cancer cells as a source of iso-genic, iso-phenotypic cell lines with or without functional p53
Inoue et al. Regulation of fibronectin expression and splicing in migrating epithelial cells: migrating MDCK cells produce a lesser amount of, but more active, fibronectin
Webb et al. Epidermal stem cells
Steinberg Laryngeal papillomatosis is associated with a defect in cellular differentiation
Marshall et al. Anion channels in the apical membrane of mammalian corneal epithelium primary cultures.
D'Anna et al. Elutriation of human keratinocytes and melanocytes from in vitro cultured epithelium
Denèfle et al. Pigment cell localizations in anuran ventral skin at climactic metamorphosis

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: DE ACORDO COM O ART. 36 DA LPI (LEI 9279/96) 2O, O PEDIDO VOLTA A EXAME SENDO CONSIDERADO NAO PATENTEAVEL PELAS RAZOES EXPOSTAS NO PARECER TECNICO ANTERIOR. PORTANTO, INDEFIRO O PRESENTE PEDIDO DE PATENTE COMO INVENCAO - DE ACORDO COM OS ARTIGOS 8O, 11, 13 E 18 (III) DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.