IT1316995B1 - Procedimento per ottenere da colture primarie di cheratinociti umanilinee cellulari immortalizzate. - Google Patents

Procedimento per ottenere da colture primarie di cheratinociti umanilinee cellulari immortalizzate.

Info

Publication number
IT1316995B1
IT1316995B1 IT2000RM000095A ITRM20000095A IT1316995B1 IT 1316995 B1 IT1316995 B1 IT 1316995B1 IT 2000RM000095 A IT2000RM000095 A IT 2000RM000095A IT RM20000095 A ITRM20000095 A IT RM20000095A IT 1316995 B1 IT1316995 B1 IT 1316995B1
Authority
IT
Italy
Prior art keywords
humanineine
keratinocytes
procedure
immortalized cell
primary cultures
Prior art date
Application number
IT2000RM000095A
Other languages
English (en)
Inventor
Luca Michele De
Elena Dellambra
Original Assignee
Idi Irccs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Idi Irccs filed Critical Idi Irccs
Priority to IT2000RM000095A priority Critical patent/IT1316995B1/it
Publication of ITRM20000095A0 publication Critical patent/ITRM20000095A0/it
Priority to EP01830116A priority patent/EP1127942A1/en
Priority to US09/790,808 priority patent/US20010018213A1/en
Publication of ITRM20000095A1 publication Critical patent/ITRM20000095A1/it
Application granted granted Critical
Publication of IT1316995B1 publication Critical patent/IT1316995B1/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Dermatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
IT2000RM000095A 2000-02-25 2000-02-25 Procedimento per ottenere da colture primarie di cheratinociti umanilinee cellulari immortalizzate. IT1316995B1 (it)

Priority Applications (3)

Application Number Priority Date Filing Date Title
IT2000RM000095A IT1316995B1 (it) 2000-02-25 2000-02-25 Procedimento per ottenere da colture primarie di cheratinociti umanilinee cellulari immortalizzate.
EP01830116A EP1127942A1 (en) 2000-02-25 2001-02-21 Method of immortalization of human keratinocytes by down-regulation of 14-3-3 sigma expression
US09/790,808 US20010018213A1 (en) 2000-02-25 2001-02-23 Method to obtain immortalized cell lines from primary cultured human keratinocytes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT2000RM000095A IT1316995B1 (it) 2000-02-25 2000-02-25 Procedimento per ottenere da colture primarie di cheratinociti umanilinee cellulari immortalizzate.

Publications (3)

Publication Number Publication Date
ITRM20000095A0 ITRM20000095A0 (it) 2000-02-25
ITRM20000095A1 ITRM20000095A1 (it) 2001-08-25
IT1316995B1 true IT1316995B1 (it) 2003-05-26

Family

ID=11454473

Family Applications (1)

Application Number Title Priority Date Filing Date
IT2000RM000095A IT1316995B1 (it) 2000-02-25 2000-02-25 Procedimento per ottenere da colture primarie di cheratinociti umanilinee cellulari immortalizzate.

Country Status (3)

Country Link
US (1) US20010018213A1 (it)
EP (1) EP1127942A1 (it)
IT (1) IT1316995B1 (it)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335156B1 (en) * 1997-12-18 2002-01-01 The Johns Hopkins University School Of Medicine 14-3-3σ arrests the cell cycle
ES2226383T3 (es) * 1998-04-17 2005-03-16 Societe Des Produits Nestle S.A. Linea de celulas inmortalizadas derivadas de tejidos cutaneos humanos normales.

Also Published As

Publication number Publication date
EP1127942A1 (en) 2001-08-29
US20010018213A1 (en) 2001-08-30
EP1127942A8 (en) 2002-01-16
ITRM20000095A1 (it) 2001-08-25
ITRM20000095A0 (it) 2000-02-25

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