NO301081B1 - Subtilisinmutant med endret proteolytisk aktivitet eller stabilitet, DNA som koder for mutanter, ekspresjonsvektor, vertscelle samt enzymatisk renseblanding i stand til å nedbryte proteiner - Google Patents
Subtilisinmutant med endret proteolytisk aktivitet eller stabilitet, DNA som koder for mutanter, ekspresjonsvektor, vertscelle samt enzymatisk renseblanding i stand til å nedbryte proteiner Download PDFInfo
- Publication number
- NO301081B1 NO301081B1 NO912567A NO912567A NO301081B1 NO 301081 B1 NO301081 B1 NO 301081B1 NO 912567 A NO912567 A NO 912567A NO 912567 A NO912567 A NO 912567A NO 301081 B1 NO301081 B1 NO 301081B1
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- Prior art keywords
- subtilisin
- amino acid
- bacillus
- mutant
- precursor
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- 238000002715 modification method Methods 0.000 description 1
- GNOLWGAJQVLBSM-UHFFFAOYSA-N n,n,5,7-tetramethyl-1,2,3,4-tetrahydronaphthalen-1-amine Chemical compound C1=C(C)C=C2C(N(C)C)CCCC2=C1C GNOLWGAJQVLBSM-UHFFFAOYSA-N 0.000 description 1
- 238000002966 oligonucleotide array Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229940066734 peptide hydrolases Drugs 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38609—Protease or amylase in solid compositions only
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US42988289A | 1989-10-31 | 1989-10-31 | |
| US07/600,430 US5185258A (en) | 1984-05-29 | 1990-10-19 | Subtilisin mutants |
| PCT/US1990/006084 WO1991006637A1 (fr) | 1989-10-31 | 1990-10-26 | Mutants de subtilisine |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO912567L NO912567L (no) | 1991-06-28 |
| NO912567D0 NO912567D0 (no) | 1991-06-28 |
| NO301081B1 true NO301081B1 (no) | 1997-09-08 |
Family
ID=27028376
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO912567A NO301081B1 (no) | 1989-10-31 | 1991-06-28 | Subtilisinmutant med endret proteolytisk aktivitet eller stabilitet, DNA som koder for mutanter, ekspresjonsvektor, vertscelle samt enzymatisk renseblanding i stand til å nedbryte proteiner |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US5185258A (fr) |
| EP (2) | EP0775749B2 (fr) |
| JP (1) | JP3335623B2 (fr) |
| KR (1) | KR970004938B1 (fr) |
| CN (1) | CN1052897A (fr) |
| AR (1) | AR244339A1 (fr) |
| AT (2) | ATE174961T1 (fr) |
| AU (1) | AU633376B2 (fr) |
| BR (1) | BR9006993A (fr) |
| CA (1) | CA2044630C (fr) |
| DE (2) | DE69032852T2 (fr) |
| DK (2) | DK0451244T3 (fr) |
| ES (2) | ES2242963T5 (fr) |
| FI (1) | FI103052B (fr) |
| IE (1) | IE62771B1 (fr) |
| MA (1) | MA22573A1 (fr) |
| NO (1) | NO301081B1 (fr) |
| NZ (1) | NZ235883A (fr) |
| PT (1) | PT95741B (fr) |
| TR (1) | TR25991A (fr) |
| WO (1) | WO1991006637A1 (fr) |
Families Citing this family (143)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6287841B1 (en) * | 1988-02-11 | 2001-09-11 | Genencor International, Inc. | High alkaline serine protease |
| US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
| US5352603A (en) * | 1989-08-31 | 1994-10-04 | Kali-Chemie Ag | Highly alkaline proteases |
| DK271490D0 (da) * | 1990-11-14 | 1990-11-14 | Novo Nordisk As | Detergentkomposition |
| US5482849A (en) * | 1990-12-21 | 1996-01-09 | Novo Nordisk A/S | Subtilisin mutants |
| EP0769549A3 (fr) * | 1990-12-21 | 1997-05-21 | Novo Nordisk A/S | Mutants enzymatiques présentant un faible degré de variation de la charge moléculaire dans une plage de pH |
| KR100258460B1 (ko) * | 1991-05-01 | 2000-06-01 | 한센 핀 베네드 | 안정화 효소 및 세제 조성물 |
| US5340735A (en) * | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
| DE4224125A1 (de) * | 1991-07-27 | 1993-01-28 | Solvay Enzymes Gmbh & Co Kg | Verfahren zur verbesserung der stabilitaet von enzymen und stabilisierte enzyme |
| US6300122B1 (en) | 1991-12-20 | 2001-10-09 | Genencor International | Method for applying enzyme to non-finished cellulosic-containing fabrics to improve appearance and feel characteristics |
| EP0571014B1 (fr) * | 1992-05-18 | 2004-03-31 | Genencor International, Inc. | Bactéries produisant des protéases alcalines et production de ces protéases alcalines |
| US6251144B1 (en) | 1992-06-12 | 2001-06-26 | Genencor International, Inc. | Enzymatic compositions and methods for producing stonewashed look on indigo-dyed denim fabric and garments |
| CA2139928C (fr) * | 1992-07-17 | 2005-09-06 | Leonardus Johannes Sofie Marie Mulleners | Serine proteases hautement alcalines |
| US5567601A (en) * | 1993-06-01 | 1996-10-22 | University Of Maryland | Subtilisin mutants lacking a primary calcium binding site |
| US5470733A (en) * | 1993-06-01 | 1995-11-28 | University Of Maryland | Calcium free subtilisin mutants |
| US6436690B1 (en) * | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
| US6440717B1 (en) * | 1993-09-15 | 2002-08-27 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis |
| BR9407833A (pt) * | 1993-10-14 | 1997-05-13 | Procter & Gamble | Composições alvejantes compreendendo enzimas protease |
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| CA2173105C (fr) * | 1993-10-14 | 2003-05-27 | Andre Baeck | Compositions de nettoyage contenant une protease |
| US5932532A (en) * | 1993-10-14 | 1999-08-03 | Procter & Gamble Company | Bleach compositions comprising protease enzyme |
| US6187579B1 (en) * | 1993-10-28 | 2001-02-13 | Carlsberg A/S | Customized proteases |
| ES2364774T3 (es) * | 1994-02-24 | 2011-09-14 | HENKEL AG & CO. KGAA | Enzimas mejoradas y detergentes que las contienen. |
| US6599730B1 (en) * | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
| US5922082A (en) | 1994-06-16 | 1999-07-13 | Procter & Gamble Company | Detergent composition containing wool compatible high alkaline proteases |
| EP0687733A1 (fr) * | 1994-06-16 | 1995-12-20 | The Procter & Gamble Company | Composition détergente contenant des protéases très alkalines compatible avec de la laine |
| BR9508582A (pt) * | 1994-08-11 | 1998-06-02 | Genencor Int | Composição limpadora contendo agente alvejante |
| GB9416841D0 (en) * | 1994-08-19 | 1994-10-12 | Finnfeeds Int Ltd | An enzyme feed additive and animal feed including it |
| US5578136A (en) | 1994-08-31 | 1996-11-26 | The Procter & Gamble Company | Automatic dishwashing compositions comprising quaternary substituted bleach activators |
| US6066611A (en) * | 1994-10-13 | 2000-05-23 | The Procter & Gamble Company | Bleaching compositions comprising protease enzymes |
| US6455295B1 (en) * | 1995-03-08 | 2002-09-24 | The Procter & Gamble Company | Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis |
| IL117350A0 (en) * | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
| US6475765B1 (en) * | 1995-03-09 | 2002-11-05 | Procter & Gamble Company | Subtilisin DY variants having decreased adsorption and increased hydrolysis |
| US5837517A (en) * | 1995-05-05 | 1998-11-17 | Novo Nordisk A/S | Protease variants and compositions |
| US6682924B1 (en) * | 1995-05-05 | 2004-01-27 | Novozymes A/S | Protease variants and compositions |
| US6946280B1 (en) * | 1996-03-29 | 2005-09-20 | Genencor International, Inc. | Enzyme multimer and process of producing same |
| US5985627A (en) * | 1997-02-28 | 1999-11-16 | Carlsberg Laboratory | Modified carboxypeptidase |
| US6284246B1 (en) | 1997-07-30 | 2001-09-04 | The Procter & Gamble Co. | Modified polypeptides with high activity and reduced allergenicity |
| ES2367505T3 (es) | 1997-10-23 | 2011-11-04 | Danisco Us Inc. | Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes. |
| AU2089499A (en) | 1997-12-20 | 1999-07-12 | Genencor International, Inc. | Accelerated stability test |
| PL342710A1 (en) | 1997-12-20 | 2001-07-02 | Genencor Int | Matrix granule |
| US6423517B2 (en) | 1997-12-20 | 2002-07-23 | Genecor International, Inc. | Granule containing protein and salt layered on an inert particle |
| AU745104B2 (en) | 1997-12-20 | 2002-03-14 | Genencor International, Inc. | Granule with hydrated barrier material |
| CA2313950A1 (fr) | 1997-12-24 | 1999-07-08 | James T. Kellis, Jr. | Methode amelioree pour tester une enzyme preferee et/ou une composition detergente preferee |
| US6642011B2 (en) * | 1998-04-15 | 2003-11-04 | Genencor International, Inc. | Human protease and use of such protease for pharmaceutical applications and for reducing the allergenicity of non-human proteins |
| US6936249B1 (en) * | 1998-04-15 | 2005-08-30 | Genencor International, Inc. | Proteins producing an altered immunogenic response and methods of making and using the same |
| US6835550B1 (en) | 1998-04-15 | 2004-12-28 | Genencor International, Inc. | Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins |
| CA2331199C (fr) | 1998-06-10 | 2012-10-23 | Markus Sakari Kauppinen | Mannanases isolees pour le traitement de fibres cellulosiques ou synthetiques |
| US6831053B1 (en) | 1998-10-23 | 2004-12-14 | The Procter & Gamble Company | Bleaching compositions comprising multiply-substituted protease variants |
| US6376450B1 (en) * | 1998-10-23 | 2002-04-23 | Chanchal Kumar Ghosh | Cleaning compositions containing multiply-substituted protease variants |
| NZ510838A (en) | 1998-10-27 | 2003-03-28 | Genencor Int | Matrix granule containing a protein and a starch admixture layered over an inert particle |
| MXPA01004750A (es) | 1998-11-13 | 2005-07-01 | Genencor Int | Granulo de baja densidad de lecho fluidizado. |
| US6579841B1 (en) | 1998-12-18 | 2003-06-17 | Genencor International, Inc. | Variant EGIII-like cellulase compositions |
| MXPA01006944A (es) | 1999-01-08 | 2003-09-10 | Genencor Int | Composiciones de baja densidad y materiales particulados que incluyen las mismas. |
| CA2387511A1 (fr) | 1999-10-15 | 2001-04-26 | Genencor International, Inc. | Granule contenant des proteines et formulations de granules |
| WO2001080665A1 (fr) * | 2000-04-26 | 2001-11-01 | Land O'lakes, Inc. | Procede de traitement de proteines du soja et produit a base de proteines du soja obtenu selon ledit procede |
| AU2001280968A1 (en) * | 2000-07-31 | 2002-02-13 | Menzel, Rolf | Compositions and methods for directed gene assembly |
| US6635465B1 (en) | 2000-08-04 | 2003-10-21 | Genencor International, Inc. | Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same |
| US6623949B1 (en) * | 2000-08-04 | 2003-09-23 | Genencor International, Inc. | Variant EGIII-like cellulase compositions |
| US7303907B2 (en) | 2001-01-08 | 2007-12-04 | Health Protection Agency | Degradation and detection of TSE infectivity |
| EP1241112A3 (fr) | 2001-03-15 | 2003-02-26 | The Procter & Gamble Company | Sachet à plusieurs compartiments |
| CA2441595C (fr) | 2001-03-23 | 2012-07-03 | Genencor International, Inc. | Proteines provoquant une reaction immunogene modifiee, et methodes de production et d'utilisation desdites proteines |
| US8076113B2 (en) | 2001-04-02 | 2011-12-13 | Danisco Us Inc. | Method for producing granules with reduced dust potential comprising an antifoam agent |
| DE60223148T2 (de) | 2001-06-22 | 2008-08-07 | Genencor International, Inc., Palo Alto | Granulat mit hoher schlagfestigkeit |
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| JP2005535284A (ja) * | 2001-12-31 | 2005-11-24 | ジェネンコー・インターナショナル・インク | 免疫反応の変化を生じるプロテアーゼ、ならびにその製造および利用方法 |
| CA2471972A1 (fr) * | 2001-12-31 | 2003-07-17 | Genencor International, Inc. | Proteases produisant une reponse immunologique modifiee et leurs procedes de production et d'utilisation |
| BR0306956A (pt) | 2002-01-16 | 2006-04-11 | Genencor Int | variantes de protease multiplamente substituìdas |
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- 1990-10-26 WO PCT/US1990/006084 patent/WO1991006637A1/fr active IP Right Grant
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- 1990-10-31 CN CN90108892A patent/CN1052897A/zh active Pending
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