NL1017973C2 - Inrichting. - Google Patents
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- NL1017973C2 NL1017973C2 NL1017973A NL1017973A NL1017973C2 NL 1017973 C2 NL1017973 C2 NL 1017973C2 NL 1017973 A NL1017973 A NL 1017973A NL 1017973 A NL1017973 A NL 1017973A NL 1017973 C2 NL1017973 C2 NL 1017973C2
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- cells
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Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
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| US09/568,254 US9068164B1 (en) | 1995-02-02 | 2000-05-10 | Method of preparing an undifferentiated cell |
| US56825400 | 2000-05-10 | ||
| GB0101315A GB0101315D0 (en) | 2001-01-18 | 2001-01-18 | A device |
| GB0101315 | 2001-01-18 | ||
| US27149701P | 2001-02-26 | 2001-02-26 | |
| US27149701 | 2001-02-26 | ||
| GB0107093 | 2001-03-21 | ||
| GB0107093A GB0107093D0 (en) | 2001-03-21 | 2001-03-21 | A device |
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| NL1017973A1 NL1017973A1 (nl) | 2001-11-13 |
| NL1017973C2 true NL1017973C2 (nl) | 2002-11-08 |
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| KR (2) | KR100875089B1 (ja) |
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| BE (1) | BE1014172A3 (ja) |
| BR (2) | BRPI0110751B1 (ja) |
| CA (2) | CA2446955C (ja) |
| CH (1) | CH696146B9 (ja) |
| CY (1) | CY1109515T1 (ja) |
| DE (1) | DE60139716D1 (ja) |
| DK (1) | DK1366144T3 (ja) |
| ES (2) | ES2190727B1 (ja) |
| FR (1) | FR2808800B1 (ja) |
| GB (2) | GB2375351B (ja) |
| HK (2) | HK1044172A1 (ja) |
| IL (2) | IL152737A0 (ja) |
| IT (1) | ITMI20010952A1 (ja) |
| JO (1) | JO2295B1 (ja) |
| MA (1) | MA25677A1 (ja) |
| MX (1) | MXPA02012295A (ja) |
| NL (1) | NL1017973C2 (ja) |
| NZ (2) | NZ556592A (ja) |
| SA (1) | SA01220163B1 (ja) |
| WO (1) | WO2001085917A2 (ja) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6946246B1 (en) * | 1997-04-08 | 2005-09-20 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Production of functional proteins: balance of shear stress and gravity |
| US7565647B2 (en) * | 2002-03-22 | 2009-07-21 | Sun Microsystems, Inc. | Markup compiler that outputs MIDlets |
| TWI288779B (en) * | 2002-03-28 | 2007-10-21 | Blasticon Biotech Forschung | Dedifferentiated, programmable stem cells of monocytic origin, and their production and use |
| DE10231655A1 (de) * | 2002-07-12 | 2004-02-26 | Blasticon Biotechnologische Forschung Gmbh | Transplantat-Akzeptanz induzierende Zellen monozytären Ursprungs, sowie deren Herstellung und Verwendung |
| CA2479164A1 (en) * | 2002-07-25 | 2004-02-05 | Japan Science And Technology Agency | Alternate immersion apparatus and alternate immersion method for treating biological sample |
| CN1823160A (zh) * | 2003-07-11 | 2006-08-23 | 布拉斯蒂康生物科技研究有限责任公司 | 单核细胞来源的自体的自身耐受性诱导细胞和它们在药物制剂中的用途 |
| GB0410130D0 (en) * | 2004-05-06 | 2004-06-09 | Molmed Spa | Cell preparation |
| US7259011B2 (en) * | 2004-05-20 | 2007-08-21 | Paul Lucas | Pluripotent adult stem cells |
| US7147626B2 (en) * | 2004-09-23 | 2006-12-12 | Celgene Corporation | Cord blood and placenta collection kit |
| US7909806B2 (en) * | 2004-09-23 | 2011-03-22 | Anthrogenesis Corporation | Cord blood and placenta collection kit |
| EP3354723B1 (en) | 2005-08-29 | 2023-12-13 | Technion Research & Development Foundation Ltd. | Media for culturing stem cells |
| AP2949A (en) | 2006-03-07 | 2014-07-31 | Shroff Geeta | Compositions comprising human embryonic stem cellsand their derivatives, methods of use, and method s of preparation |
| EP2733203B1 (en) | 2006-08-02 | 2018-10-10 | Technion Research & Development Foundation Ltd. | Methods of expanding embryonic stem cells in a suspension culture |
| AU2008223426A1 (en) * | 2007-03-01 | 2008-09-12 | Julie G. Allickson | Procurement, isolation and cryopreservation of endometrial/menstrual cells |
| GB0917565D0 (en) * | 2009-10-08 | 2009-11-25 | Univ Nottingham | An observation cell arrangement |
| GB2474492B (en) * | 2009-10-19 | 2014-05-21 | Tristem Trading Cyprus Ltd | Treatment using reprogrammed mature adult cells |
| ES2779048T3 (es) | 2009-11-12 | 2020-08-13 | Technion Res & Dev Foundation | Medios de cultivo, cultivos celulares y métodos de cultivo de células madre pluripotentes en un estado indiferenciado |
| EP2639294A1 (fr) * | 2012-03-15 | 2013-09-18 | CellProthera | Automate et procédé automatisé de culture cellulaire |
| EP3613859A1 (en) | 2013-10-24 | 2020-02-26 | Ospedale San Raffaele S.r.l. | Method |
| US10179896B2 (en) | 2015-05-12 | 2019-01-15 | Baker Group, LLP | Method and system for a bioartificial organ |
| KR20240103039A (ko) | 2016-05-25 | 2024-07-03 | 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) | 암 치료 방법 및 조성물 |
| US10711237B2 (en) * | 2017-08-22 | 2020-07-14 | Idex Health & Science Llc | Apparatus and methods for bioprocesses and other processes |
| IL314506A (en) | 2017-11-24 | 2024-09-01 | Inst Nat Sante Rech Med | Methods and preparations for the treatment of cancer |
| CN108889357A (zh) * | 2018-05-10 | 2018-11-27 | 刘英文 | 一种检验科样品临时存储装置 |
| CN112703011A (zh) | 2018-08-06 | 2021-04-23 | 国家医疗保健研究所 | 用于治疗癌症的方法和组合物 |
| CN111560345A (zh) * | 2019-05-10 | 2020-08-21 | 华中科技大学同济医学院附属协和医院 | 一种猪原代瓣膜内皮细胞分离纯化方法 |
| US20240279612A1 (en) | 2021-04-01 | 2024-08-22 | Institut National de la Santé et de la Recherche Médicale | Liver organoid manufacturing methods, liver organoids obtained with the same, and uses thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2297558A (en) * | 1995-02-02 | 1996-08-07 | Ilham Mohamed Sale Abuljadayel | Conversion of a more committed cell into an undifferentiated cell |
| FR2771421A1 (fr) * | 1997-11-27 | 1999-05-28 | Bertin & Cie | Dispositif d'amplification de cellules hematopoietiques et ses applications |
| FR2794130A1 (fr) * | 1999-05-26 | 2000-12-01 | Bertin Technologies Sa | Procede et dispositif de culture de cellules a applications multiples |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4058367A (en) * | 1976-05-19 | 1977-11-15 | Gilford Instrument Laboratories Inc. | Automatic asynchronous fluid processing apparatus |
| US4563907A (en) * | 1983-10-31 | 1986-01-14 | Micromedic Systems Inc. | Direct reading automatic pipette |
| US4812314A (en) * | 1986-02-24 | 1989-03-14 | Yissum Research & Dev. Co. Of The Hebrew Univ. Of Jerusalem And Hadassah Medical Organization | Lipid replacement therapy |
| JPS63196854A (ja) * | 1987-02-10 | 1988-08-15 | Toa Medical Electronics Co Ltd | リンパ球亜群の測定方法およびその装置 |
| US5004681B1 (en) | 1987-11-12 | 2000-04-11 | Biocyte Corp | Preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood |
| US5399493A (en) * | 1989-06-15 | 1995-03-21 | The Regents Of The University Of Michigan | Methods and compositions for the optimization of human hematopoietic progenitor cell cultures |
| US5763266A (en) * | 1989-06-15 | 1998-06-09 | The Regents Of The University Of Michigan | Methods, compositions and devices for maintaining and growing human stem and/or hematopoietics cells |
| US5635387A (en) * | 1990-04-23 | 1997-06-03 | Cellpro, Inc. | Methods and device for culturing human hematopoietic cells and their precursors |
| WO1993016384A1 (en) * | 1992-02-07 | 1993-08-19 | Abbott Laboratories | Method for accurately enumerating and sensitively qualifying heterogeneous cell populations in cytolytic processing conditions |
| JPH07504570A (ja) * | 1992-03-04 | 1995-05-25 | ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン | ヒトの幹細胞および／または造血細胞を維持，生育するための方法，組成物および装置 |
| EP0727216B1 (en) * | 1994-07-15 | 2003-05-28 | TAIYO KAGAKU Co., LTD. | Medicinal composition containing sialic acid derivative |
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| GB9502022D0 (en) * | 1995-02-02 | 1995-03-22 | Abuljadayel Ilham M S | A method for preparing lymphohaematopoietic progenitor cells |
| EP0807168A1 (en) * | 1995-02-02 | 1997-11-19 | Stichting Centraal Laboratorium van de Bloedtransfusiedienst van het Nederlandse Rode Kruis | Enrichment of hematopoietic stem cells from blood or bone marrow |
| US5627070A (en) * | 1995-07-26 | 1997-05-06 | Celltherapy, Inc. | Cell growing device for in vitro cell population expansion |
| US5902732A (en) * | 1995-10-04 | 1999-05-11 | Cytoscan Sciences Llc | Drug screening process measuring changes in cell volume |
| US6227202B1 (en) | 1996-09-03 | 2001-05-08 | Maulana Azad Medical College | Method of organogenesis and tissue regeneration/repair using surgical techniques |
| SK283269B6 (sk) * | 1996-11-11 | 2003-04-01 | Altana Pharma Ag | Benzonaftyridíny, farmaceutický prostriedok s ich obsahom a ich použitie |
| AU6691798A (en) * | 1997-03-07 | 1998-09-22 | Metabasis Therapeutics, Inc. | Novel indole and azaindole inhibitors of fructose-1,6-bisphosphatase |
| ES2391055T3 (es) * | 1998-02-17 | 2012-11-21 | Gamida Cell Ltd. | Procedimiento para controlar la proliferación y diferenciación de células madre y progenitoras |
| CA2344653A1 (en) * | 1998-09-29 | 2000-04-06 | Gamida Cell Ltd. | Methods of controlling proliferation and differentiation of stem and progenitor cells |
| RU2249039C2 (ru) * | 1999-02-04 | 2005-03-27 | Текнион Рисерч Энд Дивелопмент Фаундейшн Лтд. | Способ размножения/поддержания недифференцированных гемопоэтических стволовых клеток или клеток-предшественников (варианты), способ приготовления кондиционированной среды стромальных клеток, способ трансплантации недифференцированных гемопоэтических стволовых клеток или клеток-предшественников (варианты) |
| JP2002537851A (ja) * | 1999-03-09 | 2002-11-12 | アコルディス インダストリアル ファイバース ゲゼルシャフト ミット ベシュレンクテル ハフツング | 作用物質のインビトロテスト法、その装置および使用 |
| AU782396B2 (en) * | 1999-07-29 | 2005-07-21 | Steven Baranowitz | Methods for transdifferentiation of body tissues |
| US6624621B2 (en) * | 2000-04-03 | 2003-09-23 | Howard L. North, Jr. | Particle counter volume sensor |
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- 2001-05-03 FR FR0105918A patent/FR2808800B1/fr not_active Expired - Fee Related
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- 2001-05-08 BE BE2001/0321A patent/BE1014172A3/fr not_active IP Right Cessation
- 2001-05-09 ES ES200101054A patent/ES2190727B1/es not_active Expired - Fee Related
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- 2001-05-09 CH CH00833/01A patent/CH696146B9/fr not_active IP Right Cessation
- 2001-05-10 DE DE60139716T patent/DE60139716D1/de not_active Expired - Lifetime
- 2001-05-10 WO PCT/GB2001/002056 patent/WO2001085917A2/en active Application Filing
- 2001-05-10 DK DK01929796T patent/DK1366144T3/da active
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- 2001-05-10 IT IT2001MI000952A patent/ITMI20010952A1/it unknown
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- 2001-05-10 JP JP2001582506A patent/JP4860883B2/ja not_active Expired - Fee Related
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- 2001-05-10 GB GB0111488A patent/GB2366295B/en not_active Expired - Fee Related
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- 2001-05-10 CA CA2776904A patent/CA2776904A1/en not_active Abandoned
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- 2001-06-13 SA SA01220163A patent/SA01220163B1/ar unknown
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2297558A (en) * | 1995-02-02 | 1996-08-07 | Ilham Mohamed Sale Abuljadayel | Conversion of a more committed cell into an undifferentiated cell |
| FR2771421A1 (fr) * | 1997-11-27 | 1999-05-28 | Bertin & Cie | Dispositif d'amplification de cellules hematopoietiques et ses applications |
| FR2794130A1 (fr) * | 1999-05-26 | 2000-12-01 | Bertin Technologies Sa | Procede et dispositif de culture de cellules a applications multiples |
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