MX363427B - Mutante de epimerasa psicosa y metodo para preparar psicosa mediante el uso del mismo. - Google Patents

Mutante de epimerasa psicosa y metodo para preparar psicosa mediante el uso del mismo.

Info

Publication number
MX363427B
MX363427B MX2015014720A MX2015014720A MX363427B MX 363427 B MX363427 B MX 363427B MX 2015014720 A MX2015014720 A MX 2015014720A MX 2015014720 A MX2015014720 A MX 2015014720A MX 363427 B MX363427 B MX 363427B
Authority
MX
Mexico
Prior art keywords
psicose
mutant
preparing
same
epimerase mutant
Prior art date
Application number
MX2015014720A
Other languages
English (en)
Other versions
MX2015014720A (es
Inventor
Young Moon Cho
Chang Gyeom Kim
Baek Seok Lee
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of MX2015014720A publication Critical patent/MX2015014720A/es
Publication of MX363427B publication Critical patent/MX363427B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

La presente invención se refiere a: un mutante de 3-epimerasa de D-psicosa a partir de Agrobacterium tumefaciens con estabilidad térmica mejorada; un vector recombinante que comprende un gen que codifica al mutante; y un microorganismo que comprende el mutante. Además, la presente invención se relaciona a un método para producir D-psicosa mediante el uso del mutante o el microorganismo de epimerasa.
MX2015014720A 2013-04-23 2014-04-23 Mutante de epimerasa psicosa y metodo para preparar psicosa mediante el uso del mismo. MX363427B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020130044700A KR101455759B1 (ko) 2013-04-23 2013-04-23 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 제조 방법
PCT/KR2014/003545 WO2014175655A1 (ko) 2013-04-23 2014-04-23 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 제조 방법

Publications (2)

Publication Number Publication Date
MX2015014720A MX2015014720A (es) 2016-06-10
MX363427B true MX363427B (es) 2019-03-22

Family

ID=51792136

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015014720A MX363427B (es) 2013-04-23 2014-04-23 Mutante de epimerasa psicosa y metodo para preparar psicosa mediante el uso del mismo.

Country Status (12)

Country Link
US (1) US9938515B2 (es)
EP (1) EP2990483B1 (es)
JP (1) JP6200578B2 (es)
KR (1) KR101455759B1 (es)
CN (1) CN105164255B (es)
CA (1) CA2910625C (es)
DK (1) DK2990483T3 (es)
ES (1) ES2656287T3 (es)
HU (1) HUE036610T2 (es)
MX (1) MX363427B (es)
PL (1) PL2990483T3 (es)
WO (1) WO2014175655A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011033873A1 (ja) 2009-09-17 2011-03-24 富士電機ホールディングス株式会社 磁気抵抗素子及びそれを用いた不揮発性半導体記憶装置
KR102087396B1 (ko) * 2015-11-16 2020-03-10 주식회사 삼양사 과당-함유 기질로부터 사이코스를 생산하는 방법
EP3395952B1 (en) * 2015-12-23 2021-01-13 Cj Cheiljedang Corporation Composition for producing d-psicose comprising d-psicose 3-epimerase and salt and method for producing d-psicose using same
KR101723007B1 (ko) 2016-02-29 2017-04-04 씨제이제일제당(주) 고순도 d-사이코스를 제조하는 방법
CN106148311B (zh) * 2016-09-12 2019-06-21 上海立足生物科技有限公司 一种d-阿洛酮糖-3-差向异构酶的突变体及其应用
KR101919713B1 (ko) 2016-11-16 2018-11-19 씨제이제일제당 (주) 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법
CN110088277A (zh) 2016-12-14 2019-08-02 博努莫斯有限责任公司 D-阿洛酮糖的酶法生产
WO2018116266A1 (en) * 2016-12-23 2018-06-28 Petiva Private Ltd. D-psicose 3-epimerase mutant and uses thereof
KR101981430B1 (ko) 2017-06-23 2019-05-23 씨제이제일제당 (주) D-사이코스 붕산염 착물로부터 크로마토그래피를 이용한 d-사이코스의 생산 방법 및 d-사이코스를 포함하는 조성물
AR124613A1 (es) * 2017-12-08 2023-04-19 Cj Cheiljedang Corp Nueva psicosa-6-fosfato fosfatasa, composición para producir psicosa incluyendo la fosfatasa y método para producir psicosa usando la fosfatasa
US11104893B2 (en) 2018-01-24 2021-08-31 Matsutani Chemical Industry Co., Ltd. Ketose 3-epimerase with improved thermal stability
KR102439295B1 (ko) 2018-11-30 2022-09-02 씨제이제일제당 주식회사 D-사이코스 결정 및 이의 제조 방법
KR102138862B1 (ko) * 2019-03-08 2020-07-30 씨제이제일제당 주식회사 알룰로스를 생산하는 스태필로코커스 속 미생물 및 이를 이용한 알룰로스 제조방법
CN114929871A (zh) * 2019-10-31 2022-08-19 株式会社三养社 具有优异的转化活性的细胞固定化珠及制备其的方法
KR102448351B1 (ko) 2020-04-27 2022-09-28 대상 주식회사 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법
EP4257689A1 (en) 2021-12-29 2023-10-11 Daesang Corporation Novel promoter variant for constitutive expression and use thereof
CN114958815B (zh) * 2022-04-29 2023-07-25 西北工业大学 一种d-阿洛酮糖3-差向异构酶及其固定化方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100744479B1 (ko) 2005-06-01 2007-08-01 씨제이 주식회사 사이코스 에피머화 효소에 의한 사이코스의 생산 방법
KR20110035805A (ko) * 2009-09-30 2011-04-06 씨제이제일제당 (주) 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법
KR101203856B1 (ko) * 2011-08-24 2012-11-21 씨제이제일제당 (주) 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산

Also Published As

Publication number Publication date
DK2990483T3 (en) 2018-03-19
CA2910625C (en) 2018-11-06
ES2656287T3 (es) 2018-02-26
CN105164255B (zh) 2018-03-20
US20160152967A1 (en) 2016-06-02
MX2015014720A (es) 2016-06-10
US9938515B2 (en) 2018-04-10
HUE036610T2 (hu) 2018-07-30
CA2910625A1 (en) 2014-10-30
EP2990483A4 (en) 2016-11-16
PL2990483T3 (pl) 2018-06-29
EP2990483A1 (en) 2016-03-02
JP2016518135A (ja) 2016-06-23
KR101455759B1 (ko) 2014-10-28
WO2014175655A1 (ko) 2014-10-30
JP6200578B2 (ja) 2017-09-20
EP2990483B1 (en) 2017-12-13
CN105164255A (zh) 2015-12-16

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