WO2016068656A3 - 사이코스 에퍼머화 효소의 발현 시스템 및 이를 이용한 사이코스의 생산 - Google Patents

사이코스 에퍼머화 효소의 발현 시스템 및 이를 이용한 사이코스의 생산 Download PDF

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Publication number
WO2016068656A3
WO2016068656A3 PCT/KR2015/011595 KR2015011595W WO2016068656A3 WO 2016068656 A3 WO2016068656 A3 WO 2016068656A3 KR 2015011595 W KR2015011595 W KR 2015011595W WO 2016068656 A3 WO2016068656 A3 WO 2016068656A3
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WO
WIPO (PCT)
Prior art keywords
psicose
same
expression system
production
epimerase
Prior art date
Application number
PCT/KR2015/011595
Other languages
English (en)
French (fr)
Other versions
WO2016068656A2 (ko
Inventor
허진솔
김혜정
김민정
최정윤
박종진
이강표
Original Assignee
주식회사 삼양사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020140149019A external-priority patent/KR101695830B1/ko
Priority claimed from KR1020150072090A external-priority patent/KR101656063B1/ko
Application filed by 주식회사 삼양사 filed Critical 주식회사 삼양사
Priority to EP15853745.6A priority Critical patent/EP3214176B1/en
Priority to CN201580059406.XA priority patent/CN107257856B/zh
Priority to ES15853745T priority patent/ES2796749T3/es
Priority to JP2017520944A priority patent/JP6563013B2/ja
Publication of WO2016068656A2 publication Critical patent/WO2016068656A2/ko
Publication of WO2016068656A3 publication Critical patent/WO2016068656A3/ko

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)

Abstract

본 발명은 사이코스 에퍼머화 효소를 높은 안정성과 발현율로 생산할 수 있는 유전자 발현 시스템, 이를 이용한 형질전환 GRAS(Generally recognized as safe) 미생물, 상기 형질전환 GRAS 미생물 또는 형질전환 미생물로부터 얻어진 효소를 이용한 사이코스 생산방법에 관한 것이다.
PCT/KR2015/011595 2014-10-30 2015-10-30 사이코스 에퍼머화 효소의 발현 시스템 및 이를 이용한 사이코스의 생산 WO2016068656A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP15853745.6A EP3214176B1 (en) 2014-10-30 2015-10-30 Expression system of psicose epimerase and production of psicose using same
CN201580059406.XA CN107257856B (zh) 2014-10-30 2015-10-30 阿洛酮糖差向异构酶的表达系统和使用其生产阿洛酮糖
ES15853745T ES2796749T3 (es) 2014-10-30 2015-10-30 Sistema de expresión de psicosa epimerasa y producción de psicosa que utiliza la misma
JP2017520944A JP6563013B2 (ja) 2014-10-30 2015-10-30 プシコースエピメラーゼの発現システムおよびこれを用いたプシコースの生産

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR1020140149019A KR101695830B1 (ko) 2014-10-30 2014-10-30 사이코스 에퍼머화 효소의 발현 시스템 및 이를 이용한 사이코스의 생산
KR10-2014-0149019 2014-10-30
KR1020150072090A KR101656063B1 (ko) 2015-05-22 2015-05-22 사이코스 에퍼머화 효소의 발현 시스템 및 이를 이용한 사이코스의 생산
KR10-2015-0072090 2015-05-22

Publications (2)

Publication Number Publication Date
WO2016068656A2 WO2016068656A2 (ko) 2016-05-06
WO2016068656A3 true WO2016068656A3 (ko) 2016-06-23

Family

ID=55858501

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2015/011595 WO2016068656A2 (ko) 2014-10-30 2015-10-30 사이코스 에퍼머화 효소의 발현 시스템 및 이를 이용한 사이코스의 생산

Country Status (6)

Country Link
US (3) US10240140B2 (ko)
EP (1) EP3214176B1 (ko)
JP (1) JP6563013B2 (ko)
CN (1) CN107257856B (ko)
ES (1) ES2796749T3 (ko)
WO (1) WO2016068656A2 (ko)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN110862952B (zh) * 2020-01-19 2020-06-09 中国科学院天津工业生物技术研究所 5-氨基乙酰丙酸生产菌株及其构建方法和应用

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WO2018116266A1 (en) * 2016-12-23 2018-06-28 Petiva Private Ltd. D-psicose 3-epimerase mutant and uses thereof
CN108588149B (zh) * 2017-10-29 2022-06-10 中国科学院天津工业生物技术研究所 一种阿果糖浆及其制备方法
CN114350699B (zh) * 2021-12-02 2024-03-26 江南大学 一株产d-阿洛酮糖3-差向异构酶的菌株及其应用

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Publication number Priority date Publication date Assignee Title
CN110862952B (zh) * 2020-01-19 2020-06-09 中国科学院天津工业生物技术研究所 5-氨基乙酰丙酸生产菌株及其构建方法和应用

Also Published As

Publication number Publication date
US20190169591A1 (en) 2019-06-06
CN107257856A (zh) 2017-10-17
US11111485B2 (en) 2021-09-07
US10240140B2 (en) 2019-03-26
JP2017531437A (ja) 2017-10-26
US20160138005A1 (en) 2016-05-19
WO2016068656A2 (ko) 2016-05-06
EP3214176A2 (en) 2017-09-06
CN107257856B (zh) 2021-05-28
EP3214176B1 (en) 2020-04-01
US11168317B2 (en) 2021-11-09
US20190169592A1 (en) 2019-06-06
JP6563013B2 (ja) 2019-08-21
ES2796749T3 (es) 2020-11-30
EP3214176A4 (en) 2018-04-04

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