MX365497B - Sintasa isopropilmalato de nicotiana tabacum y metodo y usos de la misma. - Google Patents

Sintasa isopropilmalato de nicotiana tabacum y metodo y usos de la misma.

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Publication number
MX365497B
MX365497B MX2014002497A MX2014002497A MX365497B MX 365497 B MX365497 B MX 365497B MX 2014002497 A MX2014002497 A MX 2014002497A MX 2014002497 A MX2014002497 A MX 2014002497A MX 365497 B MX365497 B MX 365497B
Authority
MX
Mexico
Prior art keywords
seq
sequence
polynucleotide
isopropylmalate synthase
methods
Prior art date
Application number
MX2014002497A
Other languages
English (en)
Other versions
MX2014002497A (es
Inventor
Philippe Blanc Michel
Goepfert Simon
Martin Florian
Bakaher Nicolas
Nicolas Bindlerc Gregor
Original Assignee
Philip Morris Products Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products Sa filed Critical Philip Morris Products Sa
Publication of MX2014002497A publication Critical patent/MX2014002497A/es
Publication of MX365497B publication Critical patent/MX365497B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/20—Biochemical treatment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
    • C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
    • C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03013—2-Isopropylmalate synthase (2.3.3.13)

Abstract

La presente invención se refiere a una célula de planta de tabaco transgénica que comprende una construcción recombinante que comprende: (a) un polinucleótido que codifica una isopropilmalato sintasa, unido operativamente a un promotor específico de tricoma o (b) un polinucleótido que reduce o inhibe la expresión de un gen de isopropilmalato sintasa, unido operativamente a un promotor específico de tricoma; en donde (i) el polinucleótido que codifica una isopropilmatato sintasa tiene al menos 80% de identidad de secuencia con SEQ ID NO: 1 o SEQ ID NO: 10 o SEQ ID NO: 12 o SEQ ID NO: 14; o (ii) la isopropilmalato sintasa comprende un polipéptido que tiene al menos 90% de identidad de secuencia con SEQ ID NO: 2 o SEQ ID NO: 11 o SEQ ID NO: 13 o SEQ ID NO: 15, opcionalmente, en donde dicho promotor comprende la secuencia expuesta en la SEQ ID NO: 8 o una variante de la misma con al menos 60% de identidad con la misma.
MX2014002497A 2011-09-02 2012-08-31 Sintasa isopropilmalato de nicotiana tabacum y metodo y usos de la misma. MX365497B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11179882A EP2565265A1 (en) 2011-09-02 2011-09-02 Isopropylmalate synthase from Nicotiana tabacum and methods and uses thereof
PCT/EP2012/003662 WO2013029799A1 (en) 2011-09-02 2012-08-31 Isopropylmalate synthase from nicotiana tabacum and methods and uses thereof

Publications (2)

Publication Number Publication Date
MX2014002497A MX2014002497A (es) 2014-12-04
MX365497B true MX365497B (es) 2019-06-05

Family

ID=46832334

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014002497A MX365497B (es) 2011-09-02 2012-08-31 Sintasa isopropilmalato de nicotiana tabacum y metodo y usos de la misma.

Country Status (18)

Country Link
US (1) US10287599B2 (es)
EP (3) EP2565265A1 (es)
JP (2) JP2014531200A (es)
KR (1) KR102012993B1 (es)
CN (1) CN103958673B (es)
AU (1) AU2012301349B2 (es)
BR (1) BR112014004827A8 (es)
ES (1) ES2742271T3 (es)
IL (1) IL231101B (es)
IN (1) IN2014CN01706A (es)
MX (1) MX365497B (es)
MY (1) MY172423A (es)
PL (1) PL2751262T3 (es)
RU (1) RU2644238C2 (es)
SG (1) SG11201400209UA (es)
TR (1) TR201910626T4 (es)
UA (1) UA116437C2 (es)
WO (1) WO2013029799A1 (es)

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CN110312805A (zh) * 2017-02-07 2019-10-08 肯塔基大学研究基金会 方法
CN108070594B (zh) * 2017-12-22 2020-10-27 河南农业大学 烟草腺毛ttr1启动子、其表达载体及其应用
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
JP2021519064A (ja) 2018-03-28 2021-08-10 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物体における還元糖含有量の調節
EP3902390A1 (en) 2018-12-30 2021-11-03 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase
CN110610743A (zh) * 2019-08-16 2019-12-24 广东省生态环境技术研究所 一种构建铬还原酶蛋白数据库的方法
EP4041896A1 (en) 2019-10-01 2022-08-17 Philip Morris Products S.A. Modulating reducing sugar content in a plant (inv)
KR20220071260A (ko) 2019-10-01 2022-05-31 필립모리스 프로덕츠 에스.에이. 식물 내 당 및 아미노산 (sultr3) 함량 조절
US20230136941A1 (en) * 2020-04-07 2023-05-04 Board Of Trustees Of Michigan State University Improving plant nutritional value and growth through enhancement of essential amino acid levels
KR102527102B1 (ko) * 2021-03-05 2023-04-28 씨제이제일제당 주식회사 이소프로필말레이트 신타제 변이체 및 이를 이용한 l-류신의 생산 방법
WO2023036691A1 (en) 2021-09-10 2023-03-16 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants

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Also Published As

Publication number Publication date
SG11201400209UA (en) 2014-03-28
IL231101B (en) 2018-10-31
CN103958673B (zh) 2021-10-26
EP3578645A1 (en) 2019-12-11
RU2644238C2 (ru) 2018-02-08
US20140352706A1 (en) 2014-12-04
EP2751262A1 (en) 2014-07-09
EP2751262B1 (en) 2019-06-12
UA116437C2 (uk) 2018-03-26
KR20140067082A (ko) 2014-06-03
AU2012301349A1 (en) 2014-03-20
EP2565265A1 (en) 2013-03-06
ES2742271T3 (es) 2020-02-13
WO2013029799A1 (en) 2013-03-07
RU2014112234A (ru) 2015-10-10
JP2018186820A (ja) 2018-11-29
MX2014002497A (es) 2014-12-04
CN103958673A (zh) 2014-07-30
JP2014531200A (ja) 2014-11-27
BR112014004827A2 (pt) 2017-04-04
TR201910626T4 (tr) 2019-08-21
KR102012993B1 (ko) 2019-08-21
IN2014CN01706A (es) 2015-05-29
MY172423A (en) 2019-11-25
BR112014004827A8 (pt) 2018-03-13
AU2012301349B2 (en) 2015-09-17
US10287599B2 (en) 2019-05-14
IL231101A0 (en) 2014-04-30
JP7000265B2 (ja) 2022-02-04
PL2751262T3 (pl) 2020-01-31

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