MX338982B - Metodos de ensayo sin separacion. - Google Patents

Metodos de ensayo sin separacion.

Info

Publication number
MX338982B
MX338982B MX2011013319A MX2011013319A MX338982B MX 338982 B MX338982 B MX 338982B MX 2011013319 A MX2011013319 A MX 2011013319A MX 2011013319 A MX2011013319 A MX 2011013319A MX 338982 B MX338982 B MX 338982B
Authority
MX
Mexico
Prior art keywords
assays
specific binding
assay methods
methods
activator
Prior art date
Application number
MX2011013319A
Other languages
English (en)
Inventor
Hashem Akhavan-Tafti
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Publication of MX338982B publication Critical patent/MX338982B/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Plasma & Fusion (AREA)
  • Materials Engineering (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un método para detectar un analito en una muestra que comprende: a) contactar dicha muestra con un soporte sólido y un compuesto activador conjugado, en donde dicho soporte sólido comprende un compuesto quimioluminiscente y una primera parte de enlace específica para el analito, en donde dicho compuesto quimioluminiscente comprende una porción quimioluminiscente de aciertan y una porción de enlace que enlaza la porción quimioluminiscente de aciertan al soporte sólido, y dicho compuesto activador conjugado comprende una enzima de peroxidasa conjugada a una segunda parte de enlace específica para el analito. b) permitir que el analito se enlace a dicha primera parte de enlace específica y a dicha segunda parte de enlace específica formando de este modo un compuesto activador conjugado enlazado: c) después del paso b) contactar dicho compuesto activador conjugado enlazado con una solución activadora que comprende un compuesto de peróxido y compuesto activador para de este modo activar dicho compuesto quimiluminiscente y producir quimioluminiscencia; d) detectar la quimiluminiscente producida en el paso c) detectando de este modo el analito.
MX2011013319A 2006-05-09 2007-05-07 Metodos de ensayo sin separacion. MX338982B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US79883906P 2006-05-09 2006-05-09
US11/799,895 US7732153B2 (en) 2006-05-09 2007-05-03 Nonseparation assay methods
PCT/US2007/068327 WO2007133988A1 (en) 2006-05-09 2007-05-07 Nonseparation assay methods

Publications (1)

Publication Number Publication Date
MX338982B true MX338982B (es) 2016-05-06

Family

ID=38685587

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2008014293A MX2008014293A (es) 2006-05-09 2007-05-07 Metodos de ensayo sin separacion.
MX2011013319A MX338982B (es) 2006-05-09 2007-05-07 Metodos de ensayo sin separacion.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2008014293A MX2008014293A (es) 2006-05-09 2007-05-07 Metodos de ensayo sin separacion.

Country Status (9)

Country Link
US (4) US7732153B2 (es)
EP (1) EP2027453B1 (es)
JP (1) JP2009536745A (es)
KR (2) KR20090042203A (es)
CN (1) CN102175851B (es)
AU (1) AU2007249527B2 (es)
IL (1) IL194892A (es)
MX (2) MX2008014293A (es)
WO (1) WO2007133988A1 (es)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7682839B2 (en) * 2005-03-14 2010-03-23 Lumigen, Inc. Methods using novel chemiluminescent labels
US7732153B2 (en) * 2006-05-09 2010-06-08 Beckman Coulter, Inc. Nonseparation assay methods
US20100240070A1 (en) * 2007-11-07 2010-09-23 Beckman Coulter, Inc. Nonseparation Assay Methods Using Peroxide Generating Enzymes
CN101470115A (zh) * 2007-12-28 2009-07-01 万积成 同时检测10种食品微生物的装置及筛查诊断方法
EP2401392B1 (en) 2009-02-27 2017-01-25 Beckman Coulter, Inc. Solution phase homogeneous assays
US20100273189A1 (en) * 2009-02-27 2010-10-28 Beckman Coulter, Inc. Non separation assays with selective signal inhibitors
US20110097723A1 (en) * 2009-09-19 2011-04-28 Qun Liu Methods and reagents for analyte detection
CN102081102B (zh) * 2009-12-23 2013-09-18 刘萍 化学发光底物液
CN103293303B (zh) * 2012-03-01 2016-12-14 上海鑫谱生物科技有限公司 一种荧光分析方法和装置
KR101580360B1 (ko) * 2013-05-22 2015-12-28 김성천 연자성미세구조물을 이용한 리간드의 스크리닝 시스템 및 그 용도
US20160033497A1 (en) * 2014-08-02 2016-02-04 Tianxin Wang Luminescence methods and reagents for analyte detection
WO2016026453A2 (zh) * 2014-08-20 2016-02-25 北京百诺奇生物科技有限公司 一种检测样品中靶核酸的试剂盒及检测方法
CN104535560B (zh) * 2015-01-13 2017-02-01 同昕生物技术（北京）有限公司 一种酶促化学发光底物液
CN105067809A (zh) * 2015-07-27 2015-11-18 青岛汉唐生物科技有限公司 用于快速检测病原体抗体的酶免渗滤法、检测用的试剂盒及其制备方法
US11202835B2 (en) * 2015-11-20 2021-12-21 Anteo Technologies Pty Ltd Method of controlled competitive exchange
EP3176580A1 (en) 2015-12-01 2017-06-07 Roche Diagnostics GmbH Method for reduction of interferences in immunoassays
CN106483293A (zh) * 2016-10-13 2017-03-08 广州华弘生物科技有限公司 He4a 临床免疫检测试剂盒及其制备方法
CN107058584A (zh) * 2017-06-07 2017-08-18 浙江殷欣生物技术有限公司 一种核酸杂交化学发光检测方法
CN108414736B (zh) * 2018-02-08 2020-11-17 江苏三联生物工程有限公司 一种利用化学发光增强子修饰的抗体检测目标物的方法
GB2571763A (en) * 2018-03-08 2019-09-11 Sumitomo Chemical Co Enzyme-amplified lateral flow device
AU2019264344A1 (en) * 2018-05-02 2020-11-12 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Immunoassay for an automated system
WO2019221250A1 (ja) * 2018-05-17 2019-11-21 株式会社テクノメデイカ 競合イムノクロマト分析法及びイムノクロマトセンサ
CN109061176A (zh) * 2018-06-29 2018-12-21 江南大学 一种检测血清总ⅰ型胶原氨基端延长肽的试剂盒及使用方法
CN108872595A (zh) * 2018-07-05 2018-11-23 潍坊市康华生物技术有限公司 一种癌胚抗原检测试剂盒及其制备方法
US20210270838A1 (en) * 2018-07-13 2021-09-02 3M Innovative Properties Company Assay
WO2020012390A1 (en) 2018-07-13 2020-01-16 3M Innovative Properties Company Specific binding chemiluminescent assay
WO2020028619A1 (en) 2018-08-01 2020-02-06 Beckman Coulter, Inc. Sparc based microorganism detection method
CN113238055A (zh) * 2018-09-25 2021-08-10 无锡壹闪生物科技有限公司 空间邻近化学发光法检测降钙素原的试剂盒及其检测方法和应用
GB201909027D0 (en) * 2019-06-24 2019-08-07 Psyros Diagnostics Ltd A method for detecting an analyte

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4191613A (en) 1971-05-14 1980-03-04 Syva Company Malate dehydrogenase conjugates for enzyme immunoassays
US4046636A (en) 1974-06-20 1977-09-06 Syva Company Diazepam enzyme conjugates
US4067774A (en) 1971-05-14 1978-01-10 Syva Company Compounds for enzyme amplification assay
US3852157A (en) 1971-05-14 1974-12-03 Syva Corp Compounds for enzyme amplification assay
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3905871A (en) 1971-05-14 1975-09-16 Syva Co Lactam conjugates to enzymes
US4039385A (en) 1972-05-08 1977-08-02 Syva Company Cardiac glycoside enzyme conjugates
US3966556A (en) 1972-11-06 1976-06-29 Syva Company Compounds for enzyme amplification assay methadone analogs
US3875011A (en) 1972-11-06 1975-04-01 Syva Co Enzyme immunoassays with glucose-6-phosphate dehydrogenase
US3998943A (en) 1973-10-02 1976-12-21 Syva Company Double receptor fluorescent immunoassay
US4161515A (en) 1973-10-02 1979-07-17 Syva Company Double receptor fluorescent immunoassay
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US4040907A (en) 1974-06-20 1977-08-09 Syva Company Iodothyronine enzyme conjugates
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4065354A (en) 1974-10-10 1977-12-27 Syva Company Lysozyme conjugates for enzyme immunoassays
US4171244A (en) 1975-02-20 1979-10-16 Syva Company Enzyme-bound-polyidothyronine
US4043872A (en) 1975-02-20 1977-08-23 Syva Company Polyiodothyronine immunoassay
IN142734B (es) 1975-04-28 1977-08-20 Miles Lab
US4174384A (en) 1975-06-30 1979-11-13 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4130462A (en) 1976-12-16 1978-12-19 Syva Company Receptor steric hindrance immunoassay for receptor determination
IT1079045B (it) 1977-05-16 1985-05-08 Syva Co Analisi imuunologica fluorescente a doppio ricettore
US4160645A (en) 1977-07-14 1979-07-10 Syva Company Catalyst mediated competitive protein binding assay
US4208479A (en) 1977-07-14 1980-06-17 Syva Company Label modified immunoassays
US4233402A (en) 1978-04-05 1980-11-11 Syva Company Reagents and method employing channeling
US4193983A (en) 1978-05-16 1980-03-18 Syva Company Labeled liposome particle compositions and immunoassays therewith
US4226993A (en) 1978-07-24 1980-10-07 Miles Laboratories, Inc. Amino-functionalized phthalhydrazides
US4785080A (en) 1981-03-30 1988-11-15 Baker Instruments Corporation Labeled analytes
US4891324A (en) 1987-01-07 1990-01-02 Syntex (U.S.A.) Inc. Particle with luminescer for assays
JP2681061B2 (ja) * 1988-06-08 1997-11-19 ロンドン・ダイアグノスティック・インコーポレーテッド 検出可能なシグナルのセンシタイザー誘導発生を利用したアッセイ
DE68911633T2 (de) 1988-09-30 1994-04-07 Fujirebio Kk Verfahren zur Bestimmung von Peroxidase-Aktivität unter Verwendung von Chemilumineszenz.
CA2032154C (en) 1989-04-28 1996-01-09 Yoshihiro Oyama Method of high sensitivity luminescence analysis
US5324835A (en) 1990-03-30 1994-06-28 Biosensor Laboratories Co., Ltd. Pyridazinoquinoxalinones for use as chemiluminescent agents
CA2056142A1 (en) 1990-11-27 1992-05-28 Hirotomo Masuya Pyridopyridazine compounds and their use
ATE246360T1 (de) * 1991-05-22 2003-08-15 Dade Behring Marburg Gmbh Partikel, die eine zusammensetzung beinhalten, die eine chemilumineszierende verbindung umfassen
US6251581B1 (en) 1991-05-22 2001-06-26 Dade Behring Marburg Gmbh Assay method utilizing induced luminescence
US5512451A (en) 1993-04-01 1996-04-30 British Technology Group Limited Enhancement of chemiluminescent reactions
US5593845A (en) 1993-05-17 1997-01-14 Lumigen, Inc. Aryl N-alkylacridancarboxylate derivatives useful for chemiluminescent detection
US5523212A (en) 1993-05-17 1996-06-04 Lumigen, Inc. Aryl N-alkylacridanthiocarboxylate derivatives useful for chemiluminescent detection
US5491072A (en) 1993-05-17 1996-02-13 Lumigen, Inc. N-alkylacridan carboxyl derivatives useful for chemiluminescent detection
WO1995029395A1 (en) 1994-04-26 1995-11-02 The Regents Of University Of Michigan Unitary sandwich enzyme immunoassay cassette, device and method of use
CA2261032C (en) 1996-07-16 2003-11-25 De Staat Der Nederlanden, Vertegenwoordigd Door De Minister Van Welzijn, Volksgezondheid En Cultuur Dibenzodihydropyridinecarboxylic esters and their use in chemiluminescent assay methods
US5922558A (en) 1997-09-12 1999-07-13 Lumigen, Inc. Methods and compositions for generating chemiluminescence with a peroxidase
WO1999014220A1 (en) 1997-09-12 1999-03-25 Lumigen, Inc. Novel compounds for generating chemiluminescence with a peroxidase
WO2000049406A1 (en) 1999-02-16 2000-08-24 Princeton Separations Activating film for chemiluminescent assays and methods for use
JP2003149246A (ja) * 2001-08-27 2003-05-21 Kyowa Hakko Kogyo Co Ltd 物質の検出法
JP4231051B2 (ja) * 2003-08-29 2009-02-25 株式会社東芝 測定対象物質の濃度測定方法、測定対象物質の濃度測定用キット及びセンサチップ
US20060205094A1 (en) 2005-03-14 2006-09-14 Lumigen, Inc. Methods using novel chemiluminescent labels
CN1332203C (zh) * 2005-11-10 2007-08-15 上海交通大学 检测动物性食品中盐酸克伦特罗的快速测试方法
US7732153B2 (en) * 2006-05-09 2010-06-08 Beckman Coulter, Inc. Nonseparation assay methods

Also Published As

Publication number Publication date
CN102175851A (zh) 2011-09-07
US20110318747A1 (en) 2011-12-29
IL194892A (en) 2015-02-26
AU2007249527B2 (en) 2013-08-01
US20110183361A1 (en) 2011-07-28
AU2007249527A1 (en) 2007-11-22
US8124362B2 (en) 2012-02-28
US20070264664A1 (en) 2007-11-15
EP2027453B1 (en) 2017-03-15
IL194892A0 (en) 2009-08-03
US7732153B2 (en) 2010-06-08
KR20090042203A (ko) 2009-04-29
KR20150006895A (ko) 2015-01-19
EP2027453A4 (en) 2009-07-01
MX2008014293A (es) 2009-02-10
US20100317008A1 (en) 2010-12-16
CN102175851B (zh) 2015-01-07
WO2007133988A1 (en) 2007-11-22
EP2027453A1 (en) 2009-02-25
US8012705B2 (en) 2011-09-06
US7923213B2 (en) 2011-04-12
JP2009536745A (ja) 2009-10-15

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