MX2008014293A - Metodos de ensayo sin separacion. - Google Patents

Metodos de ensayo sin separacion.

Info

Publication number
MX2008014293A
MX2008014293A MX2008014293A MX2008014293A MX2008014293A MX 2008014293 A MX2008014293 A MX 2008014293A MX 2008014293 A MX2008014293 A MX 2008014293A MX 2008014293 A MX2008014293 A MX 2008014293A MX 2008014293 A MX2008014293 A MX 2008014293A
Authority
MX
Mexico
Prior art keywords
assays
specific binding
assay methods
methods
activator
Prior art date
Application number
MX2008014293A
Other languages
English (en)
Inventor
Hashem Akhavan-Tafti
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Publication of MX2008014293A publication Critical patent/MX2008014293A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Materials Engineering (AREA)
  • Biophysics (AREA)
  • Plasma & Fusion (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Se describen métodos de ensayo que implican reacciones de enlace específicas las cuales son simplificadas en comparación con métodos conocidos. Un compuesto con la capacidad de producir quimioluminiscencia, es inmovilizado en un soporte sólido ya que es un miembro de un par de enlace específico para capturar un material para análisis preferente de una muestra. Un compuesto activador que activa el compuesto quimioluminiscente y que es conjugado para un elemento de par de enlace específico, se agrega en exceso junto con la muestra al soporte sólido. La adición de una solución activadora origina una reacción quimioluminiscente en los sitios en donde el conjugado activador ha sido enlazado en forma específica. Los métodos de ensayo son denominados ensayos sin separación, debido a que no requieren la eliminación o separación de la etiqueta de detección en exceso (conjugado activador) antes del paso de detección. Los métodos aplican a varios tipos de ensayo incluyendo inmunoensayos, ensayos de receptor-ligando y ensayos de hibridación de ácido nucleico.
MX2008014293A 2006-05-09 2007-05-07 Metodos de ensayo sin separacion. MX2008014293A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US79883906P 2006-05-09 2006-05-09
US11/799,895 US7732153B2 (en) 2006-05-09 2007-05-03 Nonseparation assay methods
PCT/US2007/068327 WO2007133988A1 (en) 2006-05-09 2007-05-07 Nonseparation assay methods

Publications (1)

Publication Number Publication Date
MX2008014293A true MX2008014293A (es) 2009-02-10

Family

ID=38685587

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2011013319A MX338982B (es) 2006-05-09 2007-05-07 Metodos de ensayo sin separacion.
MX2008014293A MX2008014293A (es) 2006-05-09 2007-05-07 Metodos de ensayo sin separacion.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2011013319A MX338982B (es) 2006-05-09 2007-05-07 Metodos de ensayo sin separacion.

Country Status (9)

Country Link
US (4) US7732153B2 (es)
EP (1) EP2027453B1 (es)
JP (1) JP2009536745A (es)
KR (2) KR20150006895A (es)
CN (1) CN102175851B (es)
AU (1) AU2007249527B2 (es)
IL (1) IL194892A (es)
MX (2) MX338982B (es)
WO (1) WO2007133988A1 (es)

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CN102333886B (zh) * 2009-02-27 2018-01-19 贝克曼考尔特公司 液相均相测定
EP2401393A1 (en) * 2009-02-27 2012-01-04 Beckman Coulter, Inc. Non separation assays with selective signal inhibitors
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CN102081102B (zh) * 2009-12-23 2013-09-18 刘萍 化学发光底物液
CN103293303B (zh) * 2012-03-01 2016-12-14 上海鑫谱生物科技有限公司 一种荧光分析方法和装置
KR101580360B1 (ko) * 2013-05-22 2015-12-28 김성천 연자성미세구조물을 이용한 리간드의 스크리닝 시스템 및 그 용도
US20160033497A1 (en) * 2014-08-02 2016-02-04 Tianxin Wang Luminescence methods and reagents for analyte detection
US10494665B2 (en) 2014-08-20 2019-12-03 Huawei Yang Test kit and method for testing target nucleic acid in sample
CN104535560B (zh) * 2015-01-13 2017-02-01 同昕生物技术（北京）有限公司 一种酶促化学发光底物液
CN105067809A (zh) * 2015-07-27 2015-11-18 青岛汉唐生物科技有限公司 用于快速检测病原体抗体的酶免渗滤法、检测用的试剂盒及其制备方法
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CN106483293A (zh) * 2016-10-13 2017-03-08 广州华弘生物科技有限公司 He4a 临床免疫检测试剂盒及其制备方法
CN107058584A (zh) * 2017-06-07 2017-08-18 浙江殷欣生物技术有限公司 一种核酸杂交化学发光检测方法
CN108414736B (zh) * 2018-02-08 2020-11-17 江苏三联生物工程有限公司 一种利用化学发光增强子修饰的抗体检测目标物的方法
GB2571763A (en) * 2018-03-08 2019-09-11 Sumitomo Chemical Co Enzyme-amplified lateral flow device
CN112074739A (zh) * 2018-05-02 2020-12-11 哈恩-席卡德应用研究学会 用于自动化系统的免疫测定
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CN109061176A (zh) * 2018-06-29 2018-12-21 江南大学 一种检测血清总ⅰ型胶原氨基端延长肽的试剂盒及使用方法
CN108872595A (zh) * 2018-07-05 2018-11-23 潍坊市康华生物技术有限公司 一种癌胚抗原检测试剂盒及其制备方法
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WO2020028619A1 (en) 2018-08-01 2020-02-06 Beckman Coulter, Inc. Sparc based microorganism detection method
CN109142335A (zh) * 2018-09-25 2019-01-04 无锡壹闪生物科技有限公司 空间邻近化学发光法检测降钙素原的试剂盒及其检测方法
GB201909027D0 (en) * 2019-06-24 2019-08-07 Psyros Diagnostics Ltd A method for detecting an analyte

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Also Published As

Publication number Publication date
US20110318747A1 (en) 2011-12-29
JP2009536745A (ja) 2009-10-15
EP2027453A1 (en) 2009-02-25
AU2007249527B2 (en) 2013-08-01
EP2027453A4 (en) 2009-07-01
US8124362B2 (en) 2012-02-28
WO2007133988A1 (en) 2007-11-22
US20110183361A1 (en) 2011-07-28
CN102175851A (zh) 2011-09-07
US7732153B2 (en) 2010-06-08
IL194892A0 (en) 2009-08-03
US20070264664A1 (en) 2007-11-15
EP2027453B1 (en) 2017-03-15
AU2007249527A1 (en) 2007-11-22
CN102175851B (zh) 2015-01-07
US7923213B2 (en) 2011-04-12
US20100317008A1 (en) 2010-12-16
US8012705B2 (en) 2011-09-06
IL194892A (en) 2015-02-26
MX338982B (es) 2016-05-06
KR20090042203A (ko) 2009-04-29
KR20150006895A (ko) 2015-01-19

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