MX2021009267A - Analisis ce-ms de microfluidos naturales de la heterogeneidad de la carga de anticuerpos. - Google Patents

Analisis ce-ms de microfluidos naturales de la heterogeneidad de la carga de anticuerpos.

Info

Publication number
MX2021009267A
MX2021009267A MX2021009267A MX2021009267A MX2021009267A MX 2021009267 A MX2021009267 A MX 2021009267A MX 2021009267 A MX2021009267 A MX 2021009267A MX 2021009267 A MX2021009267 A MX 2021009267A MX 2021009267 A MX2021009267 A MX 2021009267A
Authority
MX
Mexico
Prior art keywords
antibody
antibody fragments
analysis
interest
microfluids
Prior art date
Application number
MX2021009267A
Other languages
English (en)
Spanish (es)
Inventor
Ning Li
Haibo Qiu
Hongxia Wang
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2021009267A publication Critical patent/MX2021009267A/es

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4044Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854Immunoglobulins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
MX2021009267A 2019-01-31 2020-01-30 Analisis ce-ms de microfluidos naturales de la heterogeneidad de la carga de anticuerpos. MX2021009267A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962799331P 2019-01-31 2019-01-31
US201962851365P 2019-05-22 2019-05-22
PCT/US2020/015875 WO2020160266A1 (en) 2019-01-31 2020-01-30 Native microfluidic ce-ms analysis of antibody charge heterogeneity

Publications (1)

Publication Number Publication Date
MX2021009267A true MX2021009267A (es) 2021-09-10

Family

ID=69811860

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021009267A MX2021009267A (es) 2019-01-31 2020-01-30 Analisis ce-ms de microfluidos naturales de la heterogeneidad de la carga de anticuerpos.

Country Status (12)

Country Link
US (1) US12169203B2 (https=)
EP (1) EP3918339A1 (https=)
JP (1) JP2022519246A (https=)
KR (1) KR20210122803A (https=)
CN (1) CN113646638B (https=)
AU (1) AU2020216172A1 (https=)
BR (1) BR112021013716A2 (https=)
CA (1) CA3126712A1 (https=)
IL (1) IL284929A (https=)
MX (1) MX2021009267A (https=)
SG (1) SG11202108033TA (https=)
WO (1) WO2020160266A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114544839B (zh) * 2022-01-20 2024-08-20 未名生物医药有限公司 一种抗人神经生长因子抗体的电荷变异体检测方法
KR20240164561A (ko) * 2022-03-18 2024-11-19 리제너론 파아마슈티컬스, 인크. 폴리펩타이드 변종을 분석하기 위한 방법 및 시스템
WO2024020199A1 (en) * 2022-07-21 2024-01-25 Regeneron Pharmaceuticals, Inc. Native microfluidic ce-ms analysis of antibody charge heterogeneity
WO2025022284A1 (en) * 2023-07-25 2025-01-30 Dh Technologies Development Pte. Ltd. Method for in-capillary dissociation and analysis of mass spectral data

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2370749A1 (en) * 1999-04-20 2000-10-26 Target Discovery, Inc. Polypeptide fingerprinting methods, metabolic profiling, and bioinformatics database
FR2862134B1 (fr) * 2003-11-12 2007-07-27 Sebia Sa Analyse et typage de proteines monoclonales par electrophorese capillaire et immunodeplacement
CN101201355A (zh) * 2006-12-15 2008-06-18 许洋 免疫组质谱检测个体化肿瘤生物标志及疗效试剂盒和方法
US20100187113A1 (en) * 2009-01-26 2010-07-29 Vladislav Dolnik Capillary sieving electrophoresis with a cationic surfactant for size separation of proteins
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
CN103033628A (zh) * 2012-12-18 2013-04-10 北京科技大学 制备个体全蛋白质组芯片的方法及其应用
EP3885368A1 (en) * 2015-05-12 2021-09-29 Regeneron Pharmaceuticals, Inc. Multimeric protein purity determination
GB201603291D0 (en) * 2016-02-25 2016-04-13 Binding Site Group The Ltd Antibodies
EP3602026B1 (en) * 2017-03-30 2025-10-08 908 Devices Inc. Microfluidic analysis of biological samples

Also Published As

Publication number Publication date
AU2020216172A1 (en) 2021-08-05
WO2020160266A1 (en) 2020-08-06
JP2022519246A (ja) 2022-03-22
US20200249241A1 (en) 2020-08-06
IL284929A (en) 2021-09-30
EP3918339A1 (en) 2021-12-08
SG11202108033TA (en) 2021-08-30
CN113646638B (zh) 2024-05-24
BR112021013716A2 (pt) 2021-09-21
US12169203B2 (en) 2024-12-17
KR20210122803A (ko) 2021-10-12
CA3126712A1 (en) 2020-08-06
CN113646638A (zh) 2021-11-12

Similar Documents

Publication Publication Date Title
Khataee et al. Frontiers in conventional and nanomaterials based electrochemical sensing and biosensing approaches for Ochratoxin A analysis in foodstuffs: A review
MX2021009267A (es) Analisis ce-ms de microfluidos naturales de la heterogeneidad de la carga de anticuerpos.
Tanca et al. Comparison of detergent‐based sample preparation workflows for LTQ‐O rbitrap analysis of the E scherichia coli proteome
Pitarch et al. Decoding serological response to Candida cell wall immunome into novel diagnostic, prognostic, and therapeutic candidates for systemic candidiasis by proteomic and bioinformatic analyses
ATE529752T1 (de) Antikörperquantifizierung
EA200971114A1 (ru) Определение антигенов, расположенных на эритроцитах, и антиэритроцитарных антител
Smith et al. A systematic analysis of the effects of increasing degrees of serum immunodepletion in terms of depth of coverage and other key aspects in top‐down and bottom‐up proteomic analyses
RU2011140471A (ru) Способ диагностики эндометриоза и диагностический набор для эндометриоза
JOP20230009A1 (ar) المقايسة المستندة إلى الدم لاكتشاف مرض بروتين تاو أو المرض النشواني الجيني
Hsiao et al. Development of a multiplexed assay for oral cancer candidate biomarkers using peptide immunoaffinity enrichment and targeted mass spectrometry
EA202191931A1 (ru) Способ характеризации видимых и/или невидимых частиц в биологических препаратах
Heissel et al. Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a bacterially expressed recombinant biopharmaceutical protein
Nigjeh et al. Spectral library‐based glycopeptide analysis–detection of circulating galectin‐3 binding protein in pancreatic cancer
EA202191961A1 (ru) Способ и система идентификации и количественного определения фрагментации антител
Nie et al. Deep-dive targeted quantification for ultrasensitive analysis of proteins in nondepleted human blood plasma/serum and tissues
MX2021014171A (es) Metodos para identificar y cuantificar proteinas de la celula huesped.
RU2012148244A (ru) Способ диагностики онкологических заболеваний и иммуноферментный набор для его осуществления
KR101516086B1 (ko) 대사체 분석을 이용한 류마티스 관절염 진단방법
Herwig et al. Sensitive detection of C-reactive protein in serum by immunoprecipitation–microchip capillary gel electrophoresis
BRPI0406265B8 (pt) método para análise por eletroforese capilar, método para pesquisa e tipagem de proteínas monoclonais, método de preparação de anticorpos modificados sobrecarregados negativamente e kit para detecção e tipagem de proteínas monoclonais
EP4253950A3 (en) Ce-western applications for antibody development
DE602005013947D1 (de) Antikörper gegen hundepankreaslipase
Ly et al. Peptide enrichment and protein fractionation using selective electrophoresis
RU2017120228A (ru) Особые моноклональные антитела антигена м метапневмовируса человека (hmpv) и способ их использования в методе диагностики
Li et al. Establishment of a decision tree model for diagnosis of early rheumatoid arthritis by proteomic fingerprinting