AU2020216172A1 - Native microfluidic CE-MS analysis of antibody charge heterogeneity - Google Patents

Native microfluidic CE-MS analysis of antibody charge heterogeneity Download PDF

Info

Publication number
AU2020216172A1
AU2020216172A1 AU2020216172A AU2020216172A AU2020216172A1 AU 2020216172 A1 AU2020216172 A1 AU 2020216172A1 AU 2020216172 A AU2020216172 A AU 2020216172A AU 2020216172 A AU2020216172 A AU 2020216172A AU 2020216172 A1 AU2020216172 A1 AU 2020216172A1
Authority
AU
Australia
Prior art keywords
antibody
interest
sample
post
antibody fragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2020216172A
Other languages
English (en)
Inventor
Ning Li
Haibo Qiu
Hongxia Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Regeneron Pharmaceuticals Inc
Original Assignee
Regeneron Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Publication of AU2020216172A1 publication Critical patent/AU2020216172A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2020216172A 2019-01-31 2020-01-30 Native microfluidic CE-MS analysis of antibody charge heterogeneity Abandoned AU2020216172A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962799331P 2019-01-31 2019-01-31
US62/799,331 2019-01-31
US201962851365P 2019-05-22 2019-05-22
US62/851,365 2019-05-22
PCT/US2020/015875 WO2020160266A1 (en) 2019-01-31 2020-01-30 Native microfluidic ce-ms analysis of antibody charge heterogeneity

Publications (1)

Publication Number Publication Date
AU2020216172A1 true AU2020216172A1 (en) 2021-08-05

Family

ID=69811860

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020216172A Abandoned AU2020216172A1 (en) 2019-01-31 2020-01-30 Native microfluidic CE-MS analysis of antibody charge heterogeneity

Country Status (12)

Country Link
US (1) US12169203B2 (https=)
EP (1) EP3918339A1 (https=)
JP (1) JP2022519246A (https=)
KR (1) KR20210122803A (https=)
CN (1) CN113646638B (https=)
AU (1) AU2020216172A1 (https=)
BR (1) BR112021013716A2 (https=)
CA (1) CA3126712A1 (https=)
IL (1) IL284929A (https=)
MX (1) MX2021009267A (https=)
SG (1) SG11202108033TA (https=)
WO (1) WO2020160266A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114544839B (zh) * 2022-01-20 2024-08-20 未名生物医药有限公司 一种抗人神经生长因子抗体的电荷变异体检测方法
KR20240164561A (ko) * 2022-03-18 2024-11-19 리제너론 파아마슈티컬스, 인크. 폴리펩타이드 변종을 분석하기 위한 방법 및 시스템
WO2024020199A1 (en) * 2022-07-21 2024-01-25 Regeneron Pharmaceuticals, Inc. Native microfluidic ce-ms analysis of antibody charge heterogeneity
WO2025022284A1 (en) * 2023-07-25 2025-01-30 Dh Technologies Development Pte. Ltd. Method for in-capillary dissociation and analysis of mass spectral data

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2370749A1 (en) * 1999-04-20 2000-10-26 Target Discovery, Inc. Polypeptide fingerprinting methods, metabolic profiling, and bioinformatics database
FR2862134B1 (fr) * 2003-11-12 2007-07-27 Sebia Sa Analyse et typage de proteines monoclonales par electrophorese capillaire et immunodeplacement
CN101201355A (zh) * 2006-12-15 2008-06-18 许洋 免疫组质谱检测个体化肿瘤生物标志及疗效试剂盒和方法
US20100187113A1 (en) * 2009-01-26 2010-07-29 Vladislav Dolnik Capillary sieving electrophoresis with a cationic surfactant for size separation of proteins
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
CN103033628A (zh) * 2012-12-18 2013-04-10 北京科技大学 制备个体全蛋白质组芯片的方法及其应用
EP3885368A1 (en) * 2015-05-12 2021-09-29 Regeneron Pharmaceuticals, Inc. Multimeric protein purity determination
GB201603291D0 (en) * 2016-02-25 2016-04-13 Binding Site Group The Ltd Antibodies
EP3602026B1 (en) * 2017-03-30 2025-10-08 908 Devices Inc. Microfluidic analysis of biological samples

Also Published As

Publication number Publication date
WO2020160266A1 (en) 2020-08-06
JP2022519246A (ja) 2022-03-22
US20200249241A1 (en) 2020-08-06
IL284929A (en) 2021-09-30
EP3918339A1 (en) 2021-12-08
SG11202108033TA (en) 2021-08-30
CN113646638B (zh) 2024-05-24
BR112021013716A2 (pt) 2021-09-21
US12169203B2 (en) 2024-12-17
MX2021009267A (es) 2021-09-10
KR20210122803A (ko) 2021-10-12
CA3126712A1 (en) 2020-08-06
CN113646638A (zh) 2021-11-12

Similar Documents

Publication Publication Date Title
US12169203B2 (en) Native microfluidic CE-MS analysis of antibody charge heterogeneity
RU2476886C2 (ru) Способ характеризации рекомбинантного поликлонального белка
ES3027676T3 (en) Method and system of identifying and quantifying antibody fragmentation
JP7554312B2 (ja) 液体クロマトグラフィー－質量分析を用いるタンパク質分析のシステムおよび方法
JP2025041777A (ja) プロテインａクロマトグラフィー－エレクトロスプレーイオン化質量分析計
KR20210045449A (ko) 새로운 단백질 서열분석 방법
JP2020101538A5 (https=)
US20240053359A1 (en) Native microfluidic ce-ms analysis of antibody charge heterogeneity
JP7695250B2 (ja) 質量スペクトル分析においてシグナルを増強するためのアミノ酸の使用
US20230043610A1 (en) Native microfluidic ce-ms analysis of antibody charge heterogeneity
JP7813286B2 (ja) 生体分子中のスクランブルジスルフィドを同定するための方法
WO2024020199A1 (en) Native microfluidic ce-ms analysis of antibody charge heterogeneity
US20230018713A1 (en) Characterization of proteins by anion-exchange chromatography mass spectrometry (aex-ms)
CN114787630A (zh) 用于抗体分析的疏水相互作用色谱法联用的天然质谱法
CN118076890A (zh) 通过阴离子交换色谱质谱(aex-ms)表征蛋白质
Richter et al. Mass spectrometric analysis of protein species of biologics
CN118765372A (zh) 使用重肽进行序列变体分析
EA048699B1 (ru) Основанная на масс-спектрометрии стратегия определения связанных с продуктом вариантов биологического препарата
EA044417B1 (ru) Количественное определение и идентификация димеров в совместных составах
Biacchi et al. Top-down and middle-down approach by fraction collection enrichment using CE-UV/MALDI-MS: Application to monoclonal antibody F c/2 charge variants

Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application