JP2022519246A - 抗体電荷不均一性についてのネイティブマイクロ流体ｃｅ－ｍｓ解析法 - Google Patents

抗体電荷不均一性についてのネイティブマイクロ流体ｃｅ－ｍｓ解析法 Download PDF

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Publication number
JP2022519246A
JP2022519246A JP2021544511A JP2021544511A JP2022519246A JP 2022519246 A JP2022519246 A JP 2022519246A JP 2021544511 A JP2021544511 A JP 2021544511A JP 2021544511 A JP2021544511 A JP 2021544511A JP 2022519246 A JP2022519246 A JP 2022519246A
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antibody
interest
sample
post
charge
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JP2021544511A
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Japanese (ja)
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JP2022519246A5 (https=
Inventor
ホンシア ワン
ハイボー チュウ
ニン リ
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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Publication of JP2022519246A publication Critical patent/JP2022519246A/ja
Publication of JP2022519246A5 publication Critical patent/JP2022519246A5/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2021544511A 2019-01-31 2020-01-30 抗体電荷不均一性についてのネイティブマイクロ流体ｃｅ－ｍｓ解析法 Pending JP2022519246A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962799331P 2019-01-31 2019-01-31
US62/799,331 2019-01-31
US201962851365P 2019-05-22 2019-05-22
US62/851,365 2019-05-22
PCT/US2020/015875 WO2020160266A1 (en) 2019-01-31 2020-01-30 Native microfluidic ce-ms analysis of antibody charge heterogeneity

Publications (2)

Publication Number Publication Date
JP2022519246A true JP2022519246A (ja) 2022-03-22
JP2022519246A5 JP2022519246A5 (https=) 2023-01-26

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JP2021544511A Pending JP2022519246A (ja) 2019-01-31 2020-01-30 抗体電荷不均一性についてのネイティブマイクロ流体ｃｅ－ｍｓ解析法

Country Status (12)

Country Link
US (1) US12169203B2 (https=)
EP (1) EP3918339A1 (https=)
JP (1) JP2022519246A (https=)
KR (1) KR20210122803A (https=)
CN (1) CN113646638B (https=)
AU (1) AU2020216172A1 (https=)
BR (1) BR112021013716A2 (https=)
CA (1) CA3126712A1 (https=)
IL (1) IL284929A (https=)
MX (1) MX2021009267A (https=)
SG (1) SG11202108033TA (https=)
WO (1) WO2020160266A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114544839B (zh) * 2022-01-20 2024-08-20 未名生物医药有限公司 一种抗人神经生长因子抗体的电荷变异体检测方法
KR20240164561A (ko) * 2022-03-18 2024-11-19 리제너론 파아마슈티컬스, 인크. 폴리펩타이드 변종을 분석하기 위한 방법 및 시스템
WO2024020199A1 (en) * 2022-07-21 2024-01-25 Regeneron Pharmaceuticals, Inc. Native microfluidic ce-ms analysis of antibody charge heterogeneity
WO2025022284A1 (en) * 2023-07-25 2025-01-30 Dh Technologies Development Pte. Ltd. Method for in-capillary dissociation and analysis of mass spectral data

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010249831A (ja) * 1999-04-20 2010-11-04 Target Discovery Inc ポリペプチドフィンガープリント法、代謝プロファイル、およびバイオインフォマティクスデータベース
US20170045527A1 (en) * 2015-05-12 2017-02-16 Regeneron Pharmaceuticals, Inc. Multimeric protein purity determination
US20180284064A1 (en) * 2017-03-30 2018-10-04 908 Devices Inc. Microfluidic analysis of biological samples

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2862134B1 (fr) * 2003-11-12 2007-07-27 Sebia Sa Analyse et typage de proteines monoclonales par electrophorese capillaire et immunodeplacement
CN101201355A (zh) * 2006-12-15 2008-06-18 许洋 免疫组质谱检测个体化肿瘤生物标志及疗效试剂盒和方法
US20100187113A1 (en) * 2009-01-26 2010-07-29 Vladislav Dolnik Capillary sieving electrophoresis with a cationic surfactant for size separation of proteins
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
CN103033628A (zh) * 2012-12-18 2013-04-10 北京科技大学 制备个体全蛋白质组芯片的方法及其应用
GB201603291D0 (en) * 2016-02-25 2016-04-13 Binding Site Group The Ltd Antibodies

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010249831A (ja) * 1999-04-20 2010-11-04 Target Discovery Inc ポリペプチドフィンガープリント法、代謝プロファイル、およびバイオインフォマティクスデータベース
US20170045527A1 (en) * 2015-05-12 2017-02-16 Regeneron Pharmaceuticals, Inc. Multimeric protein purity determination
JP2018528389A (ja) * 2015-05-12 2018-09-27 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 多量体タンパク質の純度の決定
US20180284064A1 (en) * 2017-03-30 2018-10-04 908 Devices Inc. Microfluidic analysis of biological samples

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"ZipChip and Thermo Scientific MS for CE/ESI-MS Analyses", [オンライン], JPN6024023996, February 2018 (2018-02-01), ISSN: 0005617863 *
HASELBERG ET AL.: "Heterogeneity assessment of antibody-derived therapeutics at the intact and middle-up level by low-f", ANALYTICA CHIMICA ACTA, vol. 1044, JPN6023043662, 15 August 2018 (2018-08-15), pages 181 - 190, ISSN: 0005617861 *
LEW ET AL.: "Rapid Level-3 Characterization of Therapeutic Antibodies by Capillary Electrophoresis Electrospray I", JOURNAL OF CHROMATOGRAPHIC SCIENCE, vol. Vol.53/Iss.3, JPN6023043661, 12 February 2015 (2015-02-12), US, pages 443 - 449, ISSN: 0005617862 *

Also Published As

Publication number Publication date
AU2020216172A1 (en) 2021-08-05
WO2020160266A1 (en) 2020-08-06
US20200249241A1 (en) 2020-08-06
IL284929A (en) 2021-09-30
EP3918339A1 (en) 2021-12-08
SG11202108033TA (en) 2021-08-30
CN113646638B (zh) 2024-05-24
BR112021013716A2 (pt) 2021-09-21
US12169203B2 (en) 2024-12-17
MX2021009267A (es) 2021-09-10
KR20210122803A (ko) 2021-10-12
CA3126712A1 (en) 2020-08-06
CN113646638A (zh) 2021-11-12

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