BR112021013716A2 - Análise microfluídica nativa ce-ms de heterogeneidade de carga de anticorpo - Google Patents

Análise microfluídica nativa ce-ms de heterogeneidade de carga de anticorpo Download PDF

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Publication number
BR112021013716A2
BR112021013716A2 BR112021013716-5A BR112021013716A BR112021013716A2 BR 112021013716 A2 BR112021013716 A2 BR 112021013716A2 BR 112021013716 A BR112021013716 A BR 112021013716A BR 112021013716 A2 BR112021013716 A2 BR 112021013716A2
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BR
Brazil
Prior art keywords
antibody
interest
sample
antibody fragments
post
Prior art date
Application number
BR112021013716-5A
Other languages
English (en)
Portuguese (pt)
Inventor
Hongxia Wang
Haibo Qiu
Ning Li
Original Assignee
Regeneron Pharmaceuticals, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharmaceuticals, Inc. filed Critical Regeneron Pharmaceuticals, Inc.
Publication of BR112021013716A2 publication Critical patent/BR112021013716A2/pt

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BR112021013716-5A 2019-01-31 2020-01-30 Análise microfluídica nativa ce-ms de heterogeneidade de carga de anticorpo BR112021013716A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962799331P 2019-01-31 2019-01-31
US62/799,331 2019-01-31
US201962851365P 2019-05-22 2019-05-22
US62/851,365 2019-05-22
PCT/US2020/015875 WO2020160266A1 (en) 2019-01-31 2020-01-30 Native microfluidic ce-ms analysis of antibody charge heterogeneity

Publications (1)

Publication Number Publication Date
BR112021013716A2 true BR112021013716A2 (pt) 2021-09-21

Family

ID=69811860

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021013716-5A BR112021013716A2 (pt) 2019-01-31 2020-01-30 Análise microfluídica nativa ce-ms de heterogeneidade de carga de anticorpo

Country Status (12)

Country Link
US (1) US12169203B2 (https=)
EP (1) EP3918339A1 (https=)
JP (1) JP2022519246A (https=)
KR (1) KR20210122803A (https=)
CN (1) CN113646638B (https=)
AU (1) AU2020216172A1 (https=)
BR (1) BR112021013716A2 (https=)
CA (1) CA3126712A1 (https=)
IL (1) IL284929A (https=)
MX (1) MX2021009267A (https=)
SG (1) SG11202108033TA (https=)
WO (1) WO2020160266A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114544839B (zh) * 2022-01-20 2024-08-20 未名生物医药有限公司 一种抗人神经生长因子抗体的电荷变异体检测方法
KR20240164561A (ko) * 2022-03-18 2024-11-19 리제너론 파아마슈티컬스, 인크. 폴리펩타이드 변종을 분석하기 위한 방법 및 시스템
WO2024020199A1 (en) * 2022-07-21 2024-01-25 Regeneron Pharmaceuticals, Inc. Native microfluidic ce-ms analysis of antibody charge heterogeneity
WO2025022284A1 (en) * 2023-07-25 2025-01-30 Dh Technologies Development Pte. Ltd. Method for in-capillary dissociation and analysis of mass spectral data

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2370749A1 (en) * 1999-04-20 2000-10-26 Target Discovery, Inc. Polypeptide fingerprinting methods, metabolic profiling, and bioinformatics database
FR2862134B1 (fr) * 2003-11-12 2007-07-27 Sebia Sa Analyse et typage de proteines monoclonales par electrophorese capillaire et immunodeplacement
CN101201355A (zh) * 2006-12-15 2008-06-18 许洋 免疫组质谱检测个体化肿瘤生物标志及疗效试剂盒和方法
US20100187113A1 (en) * 2009-01-26 2010-07-29 Vladislav Dolnik Capillary sieving electrophoresis with a cationic surfactant for size separation of proteins
MY192182A (en) 2009-06-26 2022-08-04 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
CN103033628A (zh) * 2012-12-18 2013-04-10 北京科技大学 制备个体全蛋白质组芯片的方法及其应用
EP3885368A1 (en) * 2015-05-12 2021-09-29 Regeneron Pharmaceuticals, Inc. Multimeric protein purity determination
GB201603291D0 (en) * 2016-02-25 2016-04-13 Binding Site Group The Ltd Antibodies
EP3602026B1 (en) * 2017-03-30 2025-10-08 908 Devices Inc. Microfluidic analysis of biological samples

Also Published As

Publication number Publication date
AU2020216172A1 (en) 2021-08-05
WO2020160266A1 (en) 2020-08-06
JP2022519246A (ja) 2022-03-22
US20200249241A1 (en) 2020-08-06
IL284929A (en) 2021-09-30
EP3918339A1 (en) 2021-12-08
SG11202108033TA (en) 2021-08-30
CN113646638B (zh) 2024-05-24
US12169203B2 (en) 2024-12-17
MX2021009267A (es) 2021-09-10
KR20210122803A (ko) 2021-10-12
CA3126712A1 (en) 2020-08-06
CN113646638A (zh) 2021-11-12

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2864 DE 25-11-2025 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.