MX2021000445A - Deteccion y cuantificacion de peptidos glicosilados. - Google Patents

Deteccion y cuantificacion de peptidos glicosilados.

Info

Publication number
MX2021000445A
MX2021000445A MX2021000445A MX2021000445A MX2021000445A MX 2021000445 A MX2021000445 A MX 2021000445A MX 2021000445 A MX2021000445 A MX 2021000445A MX 2021000445 A MX2021000445 A MX 2021000445A MX 2021000445 A MX2021000445 A MX 2021000445A
Authority
MX
Mexico
Prior art keywords
glycopeptides
quantification
detection
glycosylated peptides
enrichment substrate
Prior art date
Application number
MX2021000445A
Other languages
English (en)
Inventor
Avraham Z Rosenberg
Biao Shen
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2021000445A publication Critical patent/MX2021000445A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • B01D15/305—Hydrophilic interaction chromatography [HILIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K9/00—Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/38—Post-translational modifications [PTMs] in chemical analysis of biological material addition of carbohydrates, e.g. glycosylation, glycation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un método de purificación y/o separación de glicopéptidos, y de cuantificación de estos. El método incluye poner en contacto una muestra que comprende glicopéptidos con un sustrato de enriquecimiento hidrofílico en condiciones que permitan que los glicopéptidos se unan al sustrato de enriquecimiento hidrofílico. Los glicopéptidos eluyen del sustrato de enriquecimiento hidrofílico con una solución de formiato de amonio y acetonitrilo (ACN) en agua para crear una muestra de glicopéptido enriquecida, que puede someterse a análisis para identificar glicopéptidos específicos.
MX2021000445A 2018-07-13 2019-07-12 Deteccion y cuantificacion de peptidos glicosilados. MX2021000445A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862697547P 2018-07-13 2018-07-13
PCT/US2019/041541 WO2020014572A1 (en) 2018-07-13 2019-07-12 Detection and quantification of glycosylated peptides

Publications (1)

Publication Number Publication Date
MX2021000445A true MX2021000445A (es) 2021-05-27

Family

ID=67544336

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021000445A MX2021000445A (es) 2018-07-13 2019-07-12 Deteccion y cuantificacion de peptidos glicosilados.

Country Status (15)

Country Link
US (2) US11807665B2 (es)
EP (1) EP3821256B1 (es)
JP (2) JP7412407B2 (es)
KR (1) KR20210030401A (es)
CN (2) CN117929753A (es)
AR (1) AR115779A1 (es)
AU (1) AU2019301736A1 (es)
BR (1) BR112021000520A2 (es)
CA (1) CA3105821A1 (es)
EA (1) EA202190263A1 (es)
IL (1) IL279975A (es)
MX (1) MX2021000445A (es)
SG (1) SG11202100219WA (es)
TW (1) TW202012926A (es)
WO (1) WO2020014572A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113150062B (zh) * 2021-03-01 2022-09-16 复旦大学 一种特异性分离富集内源性糖基化肽的方法
WO2022234412A1 (en) 2021-05-03 2022-11-10 Lupin Limited A process for purification of fc-fusion proteins
CN113237944B (zh) * 2021-05-27 2022-08-26 山东大学 海水中有机胺实时质谱分析的膜富集喷雾电离装置和方法
CN113567568B (zh) * 2021-06-04 2024-03-22 复仪合契(南京)仪器科技有限公司 一种电磁hplc在线糖肽或糖蛋白富集装置
KR102447238B1 (ko) 2022-03-15 2022-09-27 주식회사 셀키 N-연결형 및 o-연결형 당펩티드 통합 분석 방법 및 분석장치

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6723236B2 (en) 2002-03-19 2004-04-20 Waters Investments Limited Device for solid phase extraction and method for purifying samples prior to analysis
US20090294362A1 (en) 2008-05-30 2009-12-03 Persson Jonas Par Stationary phase for hydrophilic interaction chromatography
CN103833855A (zh) 2009-06-26 2014-06-04 瑞泽恩制药公司 容易地分离的具有天然免疫球蛋白形式的双特异性抗体
US10119944B2 (en) 2013-12-24 2018-11-06 Waters Technologies Corporation Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
JP6508342B2 (ja) 2015-08-31 2019-05-08 株式会社島津製作所 高分子化合物の定量分析方法及び該定量分析のためのデータ処理装置
US20200332028A1 (en) 2016-04-24 2020-10-22 Waters Technologies Corporation Charged surface reversed phase chromatographic materials method for analysis of glycans modified with amphipathic, strongly basic moieties
CN108072719B (zh) * 2016-11-18 2021-03-26 中国科学院大连化学物理研究所 一种富集分离糖肽的方法

Also Published As

Publication number Publication date
EA202190263A1 (ru) 2021-04-21
IL279975A (en) 2021-03-01
WO2020014572A1 (en) 2020-01-16
US11807665B2 (en) 2023-11-07
EP3821256B1 (en) 2024-06-19
CN112513640A (zh) 2021-03-16
CA3105821A1 (en) 2020-01-16
AU2019301736A1 (en) 2021-01-28
US20230406880A1 (en) 2023-12-21
US20200017544A1 (en) 2020-01-16
KR20210030401A (ko) 2021-03-17
AR115779A1 (es) 2021-02-24
JP2021530693A (ja) 2021-11-11
JP2024045122A (ja) 2024-04-02
CN117929753A (zh) 2024-04-26
CN112513640B (zh) 2024-02-02
BR112021000520A2 (pt) 2021-04-06
SG11202100219WA (en) 2021-02-25
JP7412407B2 (ja) 2024-01-12
EP3821256A1 (en) 2021-05-19
TW202012926A (zh) 2020-04-01

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