SG11202100219WA - Detection and quantification of glycosylated peptides - Google Patents

Detection and quantification of glycosylated peptides

Info

Publication number
SG11202100219WA
SG11202100219WA SG11202100219WA SG11202100219WA SG11202100219WA SG 11202100219W A SG11202100219W A SG 11202100219WA SG 11202100219W A SG11202100219W A SG 11202100219WA SG 11202100219W A SG11202100219W A SG 11202100219WA SG 11202100219W A SG11202100219W A SG 11202100219WA
Authority
SG
Singapore
Prior art keywords
quantification
detection
glycosylated peptides
glycosylated
peptides
Prior art date
Application number
SG11202100219WA
Inventor
Avraham Z Rosenberg
Biao Shen
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of SG11202100219WA publication Critical patent/SG11202100219WA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30Partition chromatography
    • B01D15/305Hydrophilic interaction chromatography [HILIC]
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/42Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424Elution mode
    • B01D15/426Specific type of solvent
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/20Partition-, reverse-phase or hydrophobic interaction chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K9/00Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/38Post-translational modifications [PTMs] in chemical analysis of biological material addition of carbohydrates, e.g. glycosylation, glycation
SG11202100219WA 2018-07-13 2019-07-12 Detection and quantification of glycosylated peptides SG11202100219WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862697547P 2018-07-13 2018-07-13
PCT/US2019/041541 WO2020014572A1 (en) 2018-07-13 2019-07-12 Detection and quantification of glycosylated peptides

Publications (1)

Publication Number Publication Date
SG11202100219WA true SG11202100219WA (en) 2021-02-25

Family

ID=67544336

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202100219WA SG11202100219WA (en) 2018-07-13 2019-07-12 Detection and quantification of glycosylated peptides

Country Status (15)

Country Link
US (2) US11807665B2 (en)
EP (1) EP3821256A1 (en)
JP (2) JP7412407B2 (en)
KR (1) KR20210030401A (en)
CN (1) CN112513640B (en)
AR (1) AR115779A1 (en)
AU (1) AU2019301736A1 (en)
BR (1) BR112021000520A2 (en)
CA (1) CA3105821A1 (en)
EA (1) EA202190263A1 (en)
IL (1) IL279975A (en)
MX (1) MX2021000445A (en)
SG (1) SG11202100219WA (en)
TW (1) TW202012926A (en)
WO (1) WO2020014572A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113150062B (en) * 2021-03-01 2022-09-16 复旦大学 Method for specific separation and enrichment of endogenous glycosylated peptides
WO2022234412A1 (en) 2021-05-03 2022-11-10 Lupin Limited A process for purification of fc-fusion proteins
CN113237944B (en) * 2021-05-27 2022-08-26 山东大学 Membrane enrichment spray ionization device and method for real-time mass spectrometry analysis of organic amine in seawater
CN113567568B (en) * 2021-06-04 2024-03-22 复仪合契(南京)仪器科技有限公司 Electromagnetic HPLC (high Performance liquid chromatography) online glycopeptide or glycoprotein enrichment device
KR102447238B1 (en) 2022-03-15 2022-09-27 주식회사 셀키 Integrated analysis method for n-linked and o-linked glycopeptides and analysis apparatus

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6723236B2 (en) 2002-03-19 2004-04-20 Waters Investments Limited Device for solid phase extraction and method for purifying samples prior to analysis
US20090294362A1 (en) * 2008-05-30 2009-12-03 Persson Jonas Par Stationary phase for hydrophilic interaction chromatography
CN102471378B (en) 2009-06-26 2014-04-02 瑞泽恩制药公司 Readily isolated bispecific antibodies with native immuneoglobulin format
US10119944B2 (en) * 2013-12-24 2018-11-06 Waters Technologies Corporation Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
JP6508342B2 (en) * 2015-08-31 2019-05-08 株式会社島津製作所 Method for quantitative analysis of polymer compound and data processor for said quantitative analysis
CN109154604B (en) * 2016-04-24 2023-07-21 沃特世科技公司 Charged surface reversed phase chromatography material method for analysis of glycans modified with amphiphilic strong basic moieties
CN108072719B (en) * 2016-11-18 2021-03-26 中国科学院大连化学物理研究所 Method for enriching and separating glycopeptide

Also Published As

Publication number Publication date
CN112513640B (en) 2024-02-02
JP2021530693A (en) 2021-11-11
CN112513640A (en) 2021-03-16
AU2019301736A1 (en) 2021-01-28
IL279975A (en) 2021-03-01
EA202190263A1 (en) 2021-04-21
BR112021000520A2 (en) 2021-04-06
US20230406880A1 (en) 2023-12-21
TW202012926A (en) 2020-04-01
EP3821256A1 (en) 2021-05-19
US11807665B2 (en) 2023-11-07
MX2021000445A (en) 2021-05-27
CA3105821A1 (en) 2020-01-16
JP2024045122A (en) 2024-04-02
US20200017544A1 (en) 2020-01-16
AR115779A1 (en) 2021-02-24
JP7412407B2 (en) 2024-01-12
WO2020014572A1 (en) 2020-01-16
KR20210030401A (en) 2021-03-17

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