MX2020007078A - Composiciones y metodos para la expresion de arns guias crispr que usan el promotor h1. - Google Patents

Composiciones y metodos para la expresion de arns guias crispr que usan el promotor h1.

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Publication number
MX2020007078A
MX2020007078A MX2020007078A MX2020007078A MX2020007078A MX 2020007078 A MX2020007078 A MX 2020007078A MX 2020007078 A MX2020007078 A MX 2020007078A MX 2020007078 A MX2020007078 A MX 2020007078A MX 2020007078 A MX2020007078 A MX 2020007078A
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Mexico
Prior art keywords
promoter
expression
methods
compositions
crispr guide
Prior art date
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MX2020007078A
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English (en)
Inventor
Vinod Ranganathan
Donald Zack
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Univ Johns Hopkins
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Publication date
Application filed by Univ Johns Hopkins filed Critical Univ Johns Hopkins
Publication of MX2020007078A publication Critical patent/MX2020007078A/es

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    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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Abstract

La materia objeto de la presente divulgación proporciona composiciones y métodos para la expresión de ARNs guías de CRISPR utilizando el promotor H1. En particular, se proporcionan composiciones y métodos para el uso del promotor H1 para expresar un ARN guía (ARNg) de CRISPR con especificidad alterada del nucleótido en 5', así como el uso de la secuencia promotora H1 como un promotor bidireccional para expresar la nucleasa Cas9 y el ARNg simultáneamente. También se proporcionan composiciones y métodos para la expresión y la regulación de la expresión ARNg in vivo mediante el uso de ribozimas de ARN y aptazimas regulables.
MX2020007078A 2014-06-16 2016-12-16 Composiciones y metodos para la expresion de arns guias crispr que usan el promotor h1. MX2020007078A (es)

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US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
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US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
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US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
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