MX2017002654A - Deteccion temprana de cancer de pulmon por clasificacion fenotipica por metilacion de adn de celulas derivadas de esputo. - Google Patents
Deteccion temprana de cancer de pulmon por clasificacion fenotipica por metilacion de adn de celulas derivadas de esputo.Info
- Publication number
- MX2017002654A MX2017002654A MX2017002654A MX2017002654A MX2017002654A MX 2017002654 A MX2017002654 A MX 2017002654A MX 2017002654 A MX2017002654 A MX 2017002654A MX 2017002654 A MX2017002654 A MX 2017002654A MX 2017002654 A MX2017002654 A MX 2017002654A
- Authority
- MX
- Mexico
- Prior art keywords
- cells
- lung cancer
- dna methylation
- sputum
- phenotyping
- Prior art date
Links
- 206010058467 Lung neoplasm malignant Diseases 0.000 title abstract 3
- 201000005202 lung cancer Diseases 0.000 title abstract 3
- 208000020816 lung neoplasm Diseases 0.000 title abstract 3
- 230000007067 DNA methylation Effects 0.000 title abstract 2
- 206010036790 Productive cough Diseases 0.000 title abstract 2
- 210000003802 sputum Anatomy 0.000 title abstract 2
- 208000024794 sputum Diseases 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 4
- 230000000241 respiratory effect Effects 0.000 abstract 2
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 201000011510 cancer Diseases 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 230000001973 epigenetic effect Effects 0.000 abstract 1
- 210000004072 lung Anatomy 0.000 abstract 1
- 238000000386 microscopy Methods 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57423—Specifically defined cancers of lung
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
- G01N2440/12—Post-translational modifications [PTMs] in chemical analysis of biological material alkylation, e.g. methylation, (iso-)prenylation, farnesylation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/50—Determining the risk of developing a disease
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Hematology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
En determinadas modalidades, la presente solicitud describe métodos para detectar cáncer de pulmón. El método incluye la caracterización de células extraídas del esputo humano, que es un sustituto de tejido valioso y una fuente de células del tracto respiratorio superior que se tornan cancerosas en las primeras etapas 5 del proceso de desarrollo del cáncer de pulmón. El método incluye la tinción de células extraídas con indicadores fluorescentes que producen un patrón específico en el núcleo de las células marcadas, que pueden visualizarse mediante microscopia de luz. El patrón es pertinente para un tipo de codificación epigenetica de ADN conocida como metilación de ADN, que cambia en células específicas del pulmón durante el desarrollo del cáncer, en comparación con las células respiratorias normales.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462043346P | 2014-08-28 | 2014-08-28 | |
| PCT/US2015/047567 WO2016033542A1 (en) | 2014-08-28 | 2015-08-28 | Early lung cancer detection by dna methylation phenotyping of sputum-derived cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2017002654A true MX2017002654A (es) | 2017-08-07 |
Family
ID=55400712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2017002654A MX2017002654A (es) | 2014-08-28 | 2015-08-28 | Deteccion temprana de cancer de pulmon por clasificacion fenotipica por metilacion de adn de celulas derivadas de esputo. |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US10961587B2 (es) |
| EP (2) | EP3186396B1 (es) |
| JP (1) | JP6696974B2 (es) |
| KR (1) | KR102412396B1 (es) |
| CN (1) | CN107002125B (es) |
| AU (1) | AU2015308620B2 (es) |
| BR (1) | BR112017004098A2 (es) |
| CA (1) | CA2959507A1 (es) |
| ES (1) | ES2959649T3 (es) |
| IL (1) | IL250830B (es) |
| MX (1) | MX2017002654A (es) |
| WO (1) | WO2016033542A1 (es) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10961587B2 (en) | 2014-08-28 | 2021-03-30 | Cedars-Sinai Medical Center | Early lung cancer detection by DNA methylation phenotyping of sputum-derived cells |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645739B (zh) * | 2016-08-22 | 2019-07-02 | 深圳华晓静生物科技有限公司 | 一种最优超平面的构建方法、动态优化系统和构建装置 |
| WO2018075873A1 (en) * | 2016-10-20 | 2018-04-26 | Cedars-Sinai Medical Center | Methods for single-cell prostate tissue classification and prediction of cancer progression |
| CN107621553B (zh) * | 2017-09-22 | 2020-04-24 | 中国科学院青岛生物能源与过程研究所 | 一种微生物放大成像检测方法 |
| US11972535B2 (en) * | 2019-04-23 | 2024-04-30 | Stellenbosch University | Method and system for visualising colocalised fluorescence signals |
| US11648251B2 (en) * | 2019-12-11 | 2023-05-16 | King Faisal Specialist Hospital & Research Centre | TTP phosphorylation as a biomarker in targeted therapy |
| CN113584168A (zh) * | 2021-07-19 | 2021-11-02 | 深圳泰莱生物科技有限公司 | 基于甲基化免疫沉淀高通量测序技术的肺癌检测方法 |
| CN114373502B (zh) * | 2022-01-07 | 2022-12-06 | 吉林大学第一医院 | 一种基于甲基化的肿瘤数据分析系统 |
| CN117092046B (zh) * | 2023-08-03 | 2024-03-08 | 首都医科大学附属北京安定医院 | 一种检测精神病患者口腔是否藏匿药物的方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5037207A (en) * | 1986-02-12 | 1991-08-06 | Ohio State University Research Foundation | Laser imaging system |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| US5784162A (en) | 1993-08-18 | 1998-07-21 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for biological research, medical diagnostics and therapy |
| UA40065A (uk) * | 1999-10-22 | 2001-07-16 | Приватне Підприємство Науково-Виробнича Фірма "Віта" | Спосіб лікування трахеїтів |
| JP3854943B2 (ja) | 2003-05-23 | 2006-12-06 | 独立行政法人科学技術振興機構 | Ｄｎａメチル化率の測定方法 |
| EP1949297B1 (en) * | 2005-10-14 | 2015-07-29 | Unisense Fertilitech A/S | Determination of a change in a cell population |
| AT503189B1 (de) | 2006-02-03 | 2008-06-15 | Univ Linz | Methode zur 5-methylcytosin-detektion |
| CA2683854A1 (en) * | 2007-04-11 | 2008-10-30 | Manel Esteller | Epigenetic biomarkers for early detection, therapeutic effectiveness, and relapse monitoring of cancer |
| US8084203B2 (en) * | 2008-05-12 | 2011-12-27 | Fundacion Centro Nacional De Investigaciones Oncologicas Carlos Iii | Methods for the determination of telomere length in a semi-automatic manner of every single cell in a immobilized cell population |
| US8488863B2 (en) * | 2008-11-06 | 2013-07-16 | Los Alamos National Security, Llc | Combinational pixel-by-pixel and object-level classifying, segmenting, and agglomerating in performing quantitative image analysis that distinguishes between healthy non-cancerous and cancerous cell nuclei and delineates nuclear, cytoplasm, and stromal material objects from stained biological tissue materials |
| US20100304380A1 (en) * | 2009-01-22 | 2010-12-02 | Epilumina, Inc. | Quantitative DNA Methylation Imaging of Cells and Tissues |
| WO2013010181A2 (en) * | 2011-07-14 | 2013-01-17 | University Of Massachusetts | Methods of diagnosing cancer using epigenetic biomarkers |
| EP3425065B1 (en) | 2011-12-13 | 2021-04-21 | Oslo Universitetssykehus HF | Methods and kits for detection of methylation status |
| TWI477282B (zh) * | 2012-05-23 | 2015-03-21 | Univ Ishou | 致熱原性外毒素ｂ胜肽疫苗 |
| US9297806B2 (en) * | 2012-08-01 | 2016-03-29 | The Johns Hopkins University | 5-hydroxymethylcytosine in human cancer |
| WO2016033542A1 (en) | 2014-08-28 | 2016-03-03 | Cedars-Sinai Medical Center | Early lung cancer detection by dna methylation phenotyping of sputum-derived cells |
-
2015
- 2015-08-28 WO PCT/US2015/047567 patent/WO2016033542A1/en active Application Filing
- 2015-08-28 CA CA2959507A patent/CA2959507A1/en active Pending
- 2015-08-28 AU AU2015308620A patent/AU2015308620B2/en active Active
- 2015-08-28 EP EP15835681.6A patent/EP3186396B1/en active Active
- 2015-08-28 US US15/507,668 patent/US10961587B2/en active Active
- 2015-08-28 ES ES15835681T patent/ES2959649T3/es active Active
- 2015-08-28 EP EP23191313.8A patent/EP4249608A3/en active Pending
- 2015-08-28 CN CN201580056975.9A patent/CN107002125B/zh active Active
- 2015-08-28 BR BR112017004098A patent/BR112017004098A2/pt not_active Application Discontinuation
- 2015-08-28 JP JP2017511687A patent/JP6696974B2/ja active Active
- 2015-08-28 MX MX2017002654A patent/MX2017002654A/es unknown
- 2015-08-28 KR KR1020177008449A patent/KR102412396B1/ko active IP Right Grant
-
2017
- 2017-02-27 IL IL250830A patent/IL250830B/en active IP Right Grant
-
2021
- 2021-02-19 US US17/180,433 patent/US11725250B2/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10961587B2 (en) | 2014-08-28 | 2021-03-30 | Cedars-Sinai Medical Center | Early lung cancer detection by DNA methylation phenotyping of sputum-derived cells |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107002125B (zh) | 2021-08-10 |
| AU2015308620B2 (en) | 2021-07-01 |
| EP3186396A1 (en) | 2017-07-05 |
| EP3186396B1 (en) | 2023-09-27 |
| EP3186396A4 (en) | 2019-01-09 |
| EP4249608A3 (en) | 2024-02-14 |
| KR20170065027A (ko) | 2017-06-12 |
| US11725250B2 (en) | 2023-08-15 |
| ES2959649T3 (es) | 2024-02-27 |
| BR112017004098A2 (pt) | 2017-12-05 |
| IL250830B (en) | 2020-08-31 |
| IL250830A0 (en) | 2017-04-30 |
| US20210172028A1 (en) | 2021-06-10 |
| AU2015308620A8 (en) | 2017-04-13 |
| US20170283881A1 (en) | 2017-10-05 |
| AU2015308620A1 (en) | 2017-03-16 |
| JP6696974B2 (ja) | 2020-05-20 |
| CN107002125A (zh) | 2017-08-01 |
| EP4249608A2 (en) | 2023-09-27 |
| KR102412396B1 (ko) | 2022-06-22 |
| JP2017526921A (ja) | 2017-09-14 |
| WO2016033542A1 (en) | 2016-03-03 |
| CA2959507A1 (en) | 2016-03-03 |
| US10961587B2 (en) | 2021-03-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2017002654A (es) | Deteccion temprana de cancer de pulmon por clasificacion fenotipica por metilacion de adn de celulas derivadas de esputo. | |
| MY188938A (en) | Therapeutic, diagnostic and prognostic methods for cancer of the bladder | |
| PH12017500556A1 (en) | Use of fgfr mutant gene panels in identifying cancer patients that will be responsive to treatment with an fgfr inhibitor | |
| MX2022012384A (es) | Composiciones y metodos para cribar mutaciones en el cancer de tiroides. | |
| EP3744859A3 (en) | Microrna biomarker for the diagnosis of gastric cancer | |
| EP3683320A3 (en) | Mirna fingerprint in the diagnosis of lung cancer | |
| HK1208501A1 (en) | Method for the diagnosis, prognosis and treatment of lung cancer metastasis | |
| WO2015094995A3 (en) | Gene signature biomarkers of tumor response to pd-1 antagonists | |
| WO2016138105A3 (en) | Assays to determine dna methylation and dna methylation markers of cancer | |
| WO2015153732A3 (en) | Use of double-stranded dna in exosomes: a novel biomarker in cancer detection | |
| MX2014000116A (es) | Metodos y acidos nucleicos para determinar el pronostico de un sujeto con cancer. | |
| BR112014025520A2 (pt) | método para estimar características de uma formação | |
| WO2014044853A8 (en) | A new method for classification of liver samples and diagnosis of focal nodule dysplasia, hepatocellular adenoma, and hepatocellular carcinoma | |
| WO2015094996A3 (en) | Pd-l1 gene signature biomarkers of tumor response to pd-1 antagonists | |
| WO2011112845A3 (en) | Methods and compositions related to a multi-methylation assay to predict patient outcome | |
| NZ621638A (en) | Dna methylation in colorectal and breast cancer diagnostic methods | |
| WO2013106844A3 (en) | Methods and compositions for the treatment and diaginosis of pancreatic cancer | |
| WO2011087709A3 (en) | Eml4-alk translocations in lung cancer | |
| WO2012154567A3 (en) | Human invasion signature for prognosis of metastatic risk | |
| WO2015091575A3 (en) | Different levels in blood cell samples of emt-markers for the diagnosis of cancer, in particular of colorectal (crc) and pancreatic (pc) cancer | |
| WO2014200926A3 (en) | Analysis of polynucleotides | |
| EP3531830A4 (en) | METHODS AND COMPOSITIONS FOR IDENTIFICATION AND TREATMENT OF PATIENTS WITH SMALL CELL LUNG CANCER | |
| BR112015020054A2 (pt) | Método de detectar resistência aos efeitos terapêuticos de um inibidor de akt em uma célula cancerosa | |
| TW201144809A (en) | Marker for gastric cancer and method for detecting gastric cancer | |
| MX2016017136A (es) | Composiciones y metodos para detectar pegivirus 2 de humano (hpgv-2). |