MX2016014222A - Celulas novedosas de vertebrados y metodos para la expresion recombinante de un polipeptido de interes. - Google Patents
Celulas novedosas de vertebrados y metodos para la expresion recombinante de un polipeptido de interes.Info
- Publication number
- MX2016014222A MX2016014222A MX2016014222A MX2016014222A MX2016014222A MX 2016014222 A MX2016014222 A MX 2016014222A MX 2016014222 A MX2016014222 A MX 2016014222A MX 2016014222 A MX2016014222 A MX 2016014222A MX 2016014222 A MX2016014222 A MX 2016014222A
- Authority
- MX
- Mexico
- Prior art keywords
- interest
- polypeptide
- cell
- vertebrate cells
- methods
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 6
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 6
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 6
- 241000251539 Vertebrata <Metazoa> Species 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 108010091175 Matriptase Proteins 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 238000003259 recombinant expression Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21109—Matriptase (3.4.21.109)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Enzymes And Modification Thereof (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente solicitud se refiere entre otras cosas a una célula aislada de vertebrado adecuada para la expresión recombinante de un polipéptido de interés, en el que la célula de vertebrado se altera para disminuir la función de la proteasa matriptasa endógena y en el que dicha célula comprende al menos un polinucleótido heterólogo que codifica un polipéptido de interés y en el que el polipéptido de interés es secretado por la célula. Se encontró que el uso de las células respectivas de vertebrados para producir un polipéptido recombinante de interés reduce significativamente la escisión del polipéptido de interés que se secreta en el medio de cultivo celular. También se proporcionan métodos mejorados de producción y de detección.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985589P | 2014-04-29 | 2014-04-29 | |
| US201461994310P | 2014-05-16 | 2014-05-16 | |
| PCT/IB2015/053110 WO2015166427A2 (en) | 2014-04-29 | 2015-04-29 | Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016014222A true MX2016014222A (es) | 2017-05-04 |
| MX368664B MX368664B (es) | 2019-10-10 |
Family
ID=53682742
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016014222A MX368664B (es) | 2014-04-29 | 2015-04-29 | Células novedosas de vertebrados y métodos para la expresión recombinante de un polipéptido de interés. |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US11512335B2 (es) |
| EP (3) | EP4332231A3 (es) |
| JP (1) | JP6636453B2 (es) |
| KR (1) | KR102307278B1 (es) |
| CN (1) | CN106255749A (es) |
| AU (1) | AU2015254913B2 (es) |
| CA (1) | CA2947198C (es) |
| DK (2) | DK3536776T3 (es) |
| ES (2) | ES2731246T3 (es) |
| IL (1) | IL248184B (es) |
| LT (2) | LT3137595T (es) |
| MX (1) | MX368664B (es) |
| PL (2) | PL3536776T3 (es) |
| PT (2) | PT3137595T (es) |
| RU (1) | RU2710726C2 (es) |
| SG (1) | SG11201608358VA (es) |
| WO (1) | WO2015166427A2 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3015475A1 (en) | 2014-10-31 | 2016-05-04 | Novartis AG | Mammalian cells expressing cytomegalovirus antigens |
| US10781435B2 (en) | 2017-06-22 | 2020-09-22 | Catalyst Biosciences, Inc. | Modified membrane type serine protease 1 (MTSP-1) polypeptides and methods of use |
| WO2019180607A1 (en) * | 2018-03-19 | 2019-09-26 | Centre For Cellular And Molecular Platforms | A vector, a host cell and a method thereof |
| TW202124445A (zh) | 2019-09-18 | 2021-07-01 | 瑞士商諾華公司 | Ｎｋｇ２ｄ融合蛋白及其用途 |
| JP2022522566A (ja) | 2020-02-05 | 2022-04-20 | ノバルティス アーゲー | ＣＨＯ細胞で発現されたｈｅｔ ＩＬ－１５ |
| JP2023527693A (ja) * | 2020-05-11 | 2023-06-30 | ジェネンテック， インコーポレイテッド | 神経疾患を治療するための補体成分ｃ１ｒ阻害剤、並びに関連する組成物、システム、及びそれを使用する方法 |
| WO2023223219A1 (en) * | 2022-05-19 | 2023-11-23 | Novartis Ag | IMPROVED PROTEIN PRODUCTION USING miRNA TECHNOLOGY |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5648254A (en) * | 1988-01-15 | 1997-07-15 | Zymogenetics, Inc. | Co-expression in eukaryotic cells |
| WO1992008796A1 (en) | 1990-11-13 | 1992-05-29 | Immunex Corporation | Bifunctional selectable fusion genes |
| US5371198A (en) * | 1991-12-16 | 1994-12-06 | Novo Nordisk A/S | Method for protection of proteolysis-susceptible protein during protein production in a fluid medium |
| WO1994028143A1 (en) | 1993-05-21 | 1994-12-08 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| WO2002008392A2 (en) | 2000-07-25 | 2002-01-31 | Bayer Aktiengesellschaft | Regulation of human matriptase-like serine protease |
| NZ530852A (en) * | 2001-08-27 | 2006-11-30 | Genentech Inc | Methods and compositions for recombinantly producing functional antibodies or antibody fragments in prokaryotic and eukaryotic host cells |
| JP5376747B2 (ja) * | 2002-10-02 | 2013-12-25 | カタリスト バイオサイエンシーズ， インコーポレイテッド | 改変された特異性を有するプロテアーゼを作製する方法およびスクリーニングする方法 |
| ATE399872T1 (de) | 2003-03-11 | 2008-07-15 | Serono Lab | Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten |
| US20100035240A1 (en) * | 2004-08-10 | 2010-02-11 | Cardiff Biologicals Limited | Methods and kit for the prognosis of breast cancer |
| TW200635946A (en) | 2004-12-20 | 2006-10-16 | Abgenix Inc | Binding proteins specific for human matriptase |
| US7632509B2 (en) * | 2005-07-19 | 2009-12-15 | Biosante Pharmaceuticals, Inc. | Methods to express recombinant proteins from lentiviral vectors |
| EP2178914A2 (en) | 2007-08-15 | 2010-04-28 | Bayer Schering Pharma Aktiengesellschaft | Monospecific and multispecific antibodies and method of use |
| PL2227549T3 (pl) | 2007-12-21 | 2015-12-31 | Novartis Ag | System selekcji do hodowli komórek eukariotycznych oparty na genie związanego z błoną receptora folianowego |
| US8399219B2 (en) | 2009-02-23 | 2013-03-19 | Cytomx Therapeutics, Inc. | Protease activatable interferon alpha proprotein |
| SG173456A1 (en) | 2009-02-27 | 2011-09-29 | Novartis Ag | Expression vector system comprising two selection markers |
| US8722964B2 (en) * | 2009-04-23 | 2014-05-13 | Transposagen Biopharmaceuticals, Inc. | Genetically engineered or transgenic rats exhibiting a cancer phenotype due to a disruption of germline tumor suppressor genes |
| DK2563904T3 (en) | 2010-04-26 | 2015-04-07 | Novartis Ag | Improved cell culture medium |
| WO2012162828A1 (en) | 2011-06-02 | 2012-12-06 | Socpra Sciences Santé Et Humaines S.E.C. | Matriptase inhibitors and uses thereof against orthomyxoviridae infections |
| US9120853B2 (en) * | 2012-04-27 | 2015-09-01 | Cytomx Therapeutics, Inc. | Activatable antibodies that bind epidermal growth factor receptor and methods of use thereof |
| EP2863948B1 (en) * | 2012-06-22 | 2018-10-24 | Cytomx Therapeutics Inc. | Anti-jagged 1/jagged 2 cross-reactive antibodies, activatable anti-jagged antibodies and methods of use thereof |
| EP3015475A1 (en) * | 2014-10-31 | 2016-05-04 | Novartis AG | Mammalian cells expressing cytomegalovirus antigens |
-
2015
- 2015-04-29 DK DK19161388.4T patent/DK3536776T3/da active
- 2015-04-29 US US15/307,389 patent/US11512335B2/en active Active
- 2015-04-29 PT PT15739327T patent/PT3137595T/pt unknown
- 2015-04-29 ES ES15739327T patent/ES2731246T3/es active Active
- 2015-04-29 AU AU2015254913A patent/AU2015254913B2/en active Active
- 2015-04-29 ES ES19161388T patent/ES2968879T3/es active Active
- 2015-04-29 LT LTEP15739327.3T patent/LT3137595T/lt unknown
- 2015-04-29 SG SG11201608358VA patent/SG11201608358VA/en unknown
- 2015-04-29 EP EP23205296.9A patent/EP4332231A3/en active Pending
- 2015-04-29 KR KR1020167032875A patent/KR102307278B1/ko active IP Right Grant
- 2015-04-29 JP JP2016565268A patent/JP6636453B2/ja active Active
- 2015-04-29 PT PT191613884T patent/PT3536776T/pt unknown
- 2015-04-29 RU RU2016145928A patent/RU2710726C2/ru active
- 2015-04-29 PL PL19161388.4T patent/PL3536776T3/pl unknown
- 2015-04-29 LT LTEP19161388.4T patent/LT3536776T/lt unknown
- 2015-04-29 EP EP15739327.3A patent/EP3137595B1/en active Active
- 2015-04-29 CA CA2947198A patent/CA2947198C/en active Active
- 2015-04-29 PL PL15739327T patent/PL3137595T3/pl unknown
- 2015-04-29 CN CN201580021660.0A patent/CN106255749A/zh active Pending
- 2015-04-29 MX MX2016014222A patent/MX368664B/es active IP Right Grant
- 2015-04-29 DK DK15739327.3T patent/DK3137595T3/da active
- 2015-04-29 EP EP19161388.4A patent/EP3536776B1/en active Active
- 2015-04-29 WO PCT/IB2015/053110 patent/WO2015166427A2/en active Application Filing
-
2016
- 2016-10-05 IL IL248184A patent/IL248184B/en active IP Right Grant
-
2022
- 2022-10-26 US US17/973,773 patent/US20230323419A1/en active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX368664B (es) | Células novedosas de vertebrados y métodos para la expresión recombinante de un polipéptido de interés. | |
| MX2022003477A (es) | Medio de cultivo celular complementado con taurina y metodos de uso. | |
| MX2018002705A (es) | Cepas de levadura para la expresion y secrecion de proteinas heterologas a altas temperaturas. | |
| NZ737094A (en) | Expression constructs and methods of genetically engineering methylotrophic yeast | |
| MX2021008143A (es) | Metodos para cosechar cultivos de celulas de mamifero. | |
| TR201909037T4 (tr) | Yeni protein deamidaz. | |
| MX371025B (es) | Células eucariotas novedosas y métodos para la expresión recombinante de un producto de interés. | |
| MX2015004088A (es) | Proteasas modificadas con inteina, su produccion y aplicaciones industriales. | |
| MX2018014043A (es) | Composiciones de cultivo celular con antioxidantes y metodos para la produccion de polipeptidos. | |
| MX2019003427A (es) | Composiciones para cultivo celular y metodos para la produccion de polipeptidos. | |
| SG11201901093PA (en) | Production method for insoluble recombinant protein aggregate | |
| MY182924A (en) | Enzyme compositions and uses thereof | |
| EA201791275A1 (ru) | Способы получения ctp-модифицированных полипептидов длительного действия | |
| MX2016013893A (es) | Composiciones cementicias ultralivianas y metodos relacionados. | |
| MX2020003508A (es) | Un factor de secrecion de proteinas con alta eficacia secretora y un vector de expresion que lo comprende. | |
| MX2016007207A (es) | Metodos mejorados para la produccion de polipeptidos recombinantes. | |
| MX2016008258A (es) | Celulas eucariotas novedosas y metodos para la expresion recombinante de un producto de interes. | |
| MY186789A (en) | Cathode assembly for the production of aluminum | |
| MX2018015298A (es) | Metodo para estabilizar proteinas. | |
| EP3448884C0 (en) | MANIPULATED MAMMALIAN CELLS FOR THE PRODUCTION OF RECOMBINANT PROTEINS | |
| MX369776B (es) | Vectores recombinantes del virus isfahan. | |
| MY182182A (en) | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars | |
| MX2018007298A (es) | Variantes con mejores propiedades de polipéptidos de fusión relacionados con omega-hidroxilasa. | |
| MX2022003016A (es) | Metodo de produccion con iones de cobre controlados. | |
| MX2017005295A (es) | Marcador de seleccion novedoso para transfeccion celular y produccion de proteinas. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |