MX2014013054A - Derivacion de celulas madre neurales y neuronas dopaminergicas a partir de celulas madre pluripotentes humanas. - Google Patents

Derivacion de celulas madre neurales y neuronas dopaminergicas a partir de celulas madre pluripotentes humanas.

Info

Publication number
MX2014013054A
MX2014013054A MX2014013054A MX2014013054A MX2014013054A MX 2014013054 A MX2014013054 A MX 2014013054A MX 2014013054 A MX2014013054 A MX 2014013054A MX 2014013054 A MX2014013054 A MX 2014013054A MX 2014013054 A MX2014013054 A MX 2014013054A
Authority
MX
Mexico
Prior art keywords
stem cells
human pluripotent
derivation
dopaminergic neurons
pluripotent stem
Prior art date
Application number
MX2014013054A
Other languages
English (en)
Other versions
MX368315B (es
Inventor
Gonzalez Rodolfo
Garitaonandia Ibon
MECHKIN Ruslan
Original Assignee
Int Stem Cell Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Int Stem Cell Corp filed Critical Int Stem Cell Corp
Publication of MX2014013054A publication Critical patent/MX2014013054A/es
Publication of MX368315B publication Critical patent/MX368315B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Abstract

La presente invención se basa, en parte, en un método definido desde el punto de vista químico para generar células madre neurales (NSC) y neuronas dopaminérgicas (DA) a partit de células madre pluripotentes humanas (hPSC). Las neuronas DA de la invención pueden derivar de las hPSC y las NSC. La presente invención también proporciona reactivos y kits útiles para la derivación de las células madre neurales y neuronas dopaminérgicas de células madre pluripotentes humanas.
MX2014013054A 2012-04-24 2013-04-23 Derivación de células madre neurales y neuronas dopaminérgicas a partir de células madre pluripotentes humanas. MX368315B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261637784P 2012-04-24 2012-04-24
US201261637797P 2012-04-24 2012-04-24
PCT/US2013/037860 WO2013163228A1 (en) 2012-04-24 2013-04-23 Derivation of neural stem cells and dopaminergic neurons from human pluripotent stem cells

Publications (2)

Publication Number Publication Date
MX2014013054A true MX2014013054A (es) 2016-12-20
MX368315B MX368315B (es) 2019-09-27

Family

ID=49483836

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014013054A MX368315B (es) 2012-04-24 2013-04-23 Derivación de células madre neurales y neuronas dopaminérgicas a partir de células madre pluripotentes humanas.

Country Status (13)

Country Link
US (1) US9926529B2 (es)
EP (1) EP2841566B1 (es)
JP (1) JP6262205B2 (es)
KR (1) KR20150004834A (es)
CN (1) CN104379732B (es)
AU (1) AU2013251749B2 (es)
CA (1) CA2871337C (es)
HK (1) HK1201873A1 (es)
IL (1) IL235109B (es)
MX (1) MX368315B (es)
RU (1) RU2014147015A (es)
SG (2) SG10201608838QA (es)
WO (1) WO2013163228A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010096496A2 (en) 2009-02-17 2010-08-26 Memorial Sloan-Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
EP2773748B1 (en) 2011-11-04 2019-12-25 Memorial Sloan Kettering Cancer Center Midbrain dopamine (da) neurons for engraftment
JP2016508520A (ja) * 2013-02-15 2016-03-22 インターナショナル ステム セル コーポレイション 神経変性疾患の治療のための、ヒト多能性幹細胞に由来する神経細胞の使用
CA2910394A1 (en) 2013-04-26 2014-10-30 Memorial Sloan-Kettering Cancer Center Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
US10444227B2 (en) 2014-08-19 2019-10-15 FUJIFILM Cellular Dynamics, Inc. Neural networks formed from cells derived from pluripotent stem cells
JP6980534B2 (ja) 2015-06-25 2021-12-15 ザ チルドレンズ メディカル センター コーポレーション 造血幹細胞の増大、富化、および維持に関する方法および組成物
LU92845B1 (en) * 2015-10-08 2017-05-02 Univ Du Luxembourg Campus Belval Means and methods for generating midbrain organoids
WO2017160234A1 (en) * 2016-03-14 2017-09-21 Agency For Science, Technology And Research Generation of midbrain-specific organoids from human pluripotent stem cells
DK3429603T3 (da) 2016-03-15 2022-03-14 Childrens Medical Center Fremgangsmåder og sammensætninger vedrørende ekspansion af hæmatopoietiske stamceller
EP3464569A4 (en) 2016-05-31 2020-03-11 The J. David Gladstone Institutes IN VITRO METHODS FOR DIFFERENTIATING STEM CELLS INTO NEURONES AND GENERATED NEURONES USING THE SAME IN VITRO METHODS FOR DIFFERENTIATING STEM CELLS IN NEURONES
CA3075036A1 (en) * 2017-09-07 2019-03-14 Memorial Sloan-Kettering Cancer Center Methods of differentiating stem cell-derived ectodermal lineage precursors
JP2019106895A (ja) 2017-12-15 2019-07-04 インダストリー‐ユニバーシティー コーぺレーション ファンデーション ハンヤン ユニバーシティ 神経幹細胞または神経前駆細胞からドーパミン神経細胞への分化方法
CN108559726A (zh) * 2017-12-28 2018-09-21 上海安集协康生物技术股份有限公司 一种对脑损伤类疾病有治疗作用的细胞亚群及其制备方法
KR102119148B1 (ko) * 2018-03-08 2020-06-04 고려대학교 산학협력단 신경조직 오가노이드의 제조방법, 이를 이용한 신경조직 형성 억제 물질의 스크리닝 방법 및 운동신경세포의 제조방법
EP3994250A1 (en) * 2019-07-05 2022-05-11 Novo Nordisk A/S Generation of neural stem cell lines derived from human pluripotent stem cells
CN110432888B (zh) * 2019-08-15 2021-11-23 任鹏宇 一种前庭神经权重信息编码神经元的筛选方法
JP2022549224A (ja) * 2019-09-20 2022-11-24 コリア リサーチ インスティチュート オブ バイオサイエンス アンド バイオテクノロジー ダイレクトリプログラミングを用いる、誘導型ドーパミン作動性神経前駆細胞の製造方法
JP2023503302A (ja) * 2019-11-22 2023-01-27 ノヴォ ノルディスク アー／エス スピン凝集した神経系マイクロスフェアおよびその応用
JP2021084882A (ja) * 2019-11-28 2021-06-03 学校法人慶應義塾 脊髄損傷治療用ニューロスフェア誘導剤及びその使用
CN111269887B (zh) * 2020-02-28 2021-11-16 上海市东方医院（同济大学附属东方医院） 一种人多能干细胞向外胚层分化的方法
CN117677691A (zh) * 2021-07-19 2024-03-08 特雷尔赫德生物系统股份有限公司 用于生成人中脑神经祖细胞的方法和组合物
US20230183644A1 (en) 2021-09-10 2023-06-15 FUJIFILM Cellular Dynamics, Inc. Compositions of induced pluripotent stem cell-derived cells and methods of use thereof
CA3235390A1 (en) 2021-10-29 2023-05-04 Deepika Rajesh Dopaminergic neurons comprising mutations and methods of use thereof
CN114292807A (zh) * 2021-12-28 2022-04-08 深圳市夏同生物医药科技有限公司 一种外泌体的提取方法及其应用
CN115521903A (zh) * 2022-09-30 2022-12-27 中国科学院生态环境研究中心 体外诱导人多能干细胞分化为多巴胺能神经元的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5690926A (en) 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
WO1997032033A1 (en) 1996-02-28 1997-09-04 Vanderbilt University Compositions and methods of making embryonic stem cells
ATE335490T1 (de) * 2001-10-30 2006-09-15 Novartis Pharma Gmbh Staurosporin-derivate als hemmer der flt3- rezeptor-tyrosinkinase-wirkung
US8158420B2 (en) * 2003-04-04 2012-04-17 The Trustees Of Columbia University In The City Of New York Methods for inhibiting the differentation of proliferative telencephalic cells in vitro by addition of ATF5
US20090291134A1 (en) * 2006-11-21 2009-11-26 Jina Pharmaceuticals, Inc. Endoxifen methods and compositions in the treatment of psychiatric and neurodegenerative diseases
US20110136145A1 (en) 2008-05-22 2011-06-09 The Johns Hopkins University Methods for promoting fusion and reprogramming of somatic cells
JP5761816B2 (ja) * 2009-08-12 2015-08-12 国立大学法人京都大学 多能性幹細胞から神経前駆細胞への分化誘導法
KR101168053B1 (ko) 2009-11-06 2012-07-24 연세대학교 산학협력단 효율적이고 보편적인 전분화능 줄기세포의 신경세포 분화 유도방법
US9376664B2 (en) * 2010-06-14 2016-06-28 The Scripps Research Institute Reprogramming of cells to a new fate

Also Published As

Publication number Publication date
CN104379732A (zh) 2015-02-25
IL235109B (en) 2020-05-31
CN104379732B (zh) 2018-08-17
HK1201873A1 (en) 2015-09-11
KR20150004834A (ko) 2015-01-13
SG10201608838QA (en) 2016-12-29
MX368315B (es) 2019-09-27
EP2841566B1 (en) 2019-03-13
RU2014147015A (ru) 2016-06-10
US20150087541A1 (en) 2015-03-26
AU2013251749A1 (en) 2014-11-13
EP2841566A1 (en) 2015-03-04
CA2871337C (en) 2021-04-20
JP2015514437A (ja) 2015-05-21
AU2013251749B2 (en) 2019-01-17
US9926529B2 (en) 2018-03-27
SG11201406825WA (en) 2014-11-27
WO2013163228A1 (en) 2013-10-31
EP2841566A4 (en) 2015-12-23
JP6262205B2 (ja) 2018-01-17
IL235109A0 (en) 2014-12-31
CA2871337A1 (en) 2013-10-31

Similar Documents

Publication Publication Date Title
MX368315B (es) Derivación de células madre neurales y neuronas dopaminérgicas a partir de células madre pluripotentes humanas.
IL249828A0 (en) Limbal stem cells of cultured mammals, methods of their creation and uses thereof
PH12015501477A1 (en) Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
EP3145517A4 (en) Functional oligodendrocytes derived from pluripotent stem cells and methods of making and using the same
GB2548740A (en) Methods for generation of podocytes from pluripotent stem cells and cells produced by the same
AU2016263598A8 (en) Methods and kits for treating depression
EP2582795A4 (en) METHOD FOR SELECTING HUMAN INDUCED PLURIPOTENT STEM CELLS
WO2010136583A3 (en) INDUCED DERIVATION OF SPECIFIC ENDODERM FROM hPS CELL-DERIVED DEFINITIVE ENDODERM
EP3198004A4 (en) TGFß SIGNALING INDEPENDENT NAÏVE INDUCED PLURIPOTENT STEM CELLS, METHODS OF MAKING AND USE
WO2011047103A3 (en) Cell reprogramming with applications for epigenetic and non-epigenetic base induced pluripotent stem cell derivation
SG10201903910RA (en) Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same
MX368780B (es) Matrices de micro-entornos de cultivos celulares discretos, metodos para producir estas matrices y usos de las mismas.
WO2012118988A8 (en) Direct reprogramming of human fibroblasts to functional neurons under defined conditions
PH12014501426A1 (en) Induced pluripotent stem cells from human umbilical cord tissue-derived cells
GB2523696A (en) Methods of differentiating stem cells by modulating miR-124
EP3064573A4 (en) Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into neuron
WO2015073625A3 (en) Synergistic genome-nonintegrating reprogramming by micrornas and transcription factors
EP3070159A4 (en) METHOD AND PLANT FOR CURING PLURIPOTENT STEM CELLS
MX2016014008A (es) Metodo para diferenciacion de celulas madre pluripotentes en cardiomiocitos.
EP3162812A4 (en) Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same
WO2011159050A3 (ko) 인간 신경전구세포의 도파민 뉴우런으로의 분화방법 및 분화용 배지
SG11202001138PA (en) Method for determining undifferentiated state of pluripotent stem cell, method for subculturing pluripotent stem cell, and device for use in the methods
PH12014501383A1 (en) Induced pluripotent stem cells prepared from human kidney-derived cells
SG11201903231WA (en) Microbioreactor module
MX369097B (es) Metodo para expandir las celulas madre adultas de sangre entera.

Legal Events

Date Code Title Description
FG Grant or registration