MX2008008258A - Intercambio de nucleotidos dirigido mejorado con oligonucleotidos modificados con propinilo. - Google Patents

Intercambio de nucleotidos dirigido mejorado con oligonucleotidos modificados con propinilo.

Info

Publication number
MX2008008258A
MX2008008258A MX2008008258A MX2008008258A MX2008008258A MX 2008008258 A MX2008008258 A MX 2008008258A MX 2008008258 A MX2008008258 A MX 2008008258A MX 2008008258 A MX2008008258 A MX 2008008258A MX 2008008258 A MX2008008258 A MX 2008008258A
Authority
MX
Mexico
Prior art keywords
nucleotide
modified oligonucleotides
nucleotide exchange
targeted nucleotide
dna sequence
Prior art date
Application number
MX2008008258A
Other languages
English (en)
Inventor
Paul Bundock
Both Michiel Theodoor Jan De
Rene Cornelis Josephus Hogers
Original Assignee
Keygene Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36691461&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2008008258(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Keygene Nv filed Critical Keygene Nv
Publication of MX2008008258A publication Critical patent/MX2008008258A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278—Sulfonylurea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Un método y oligonucleótidos para intercambio de nucleótido dirigido de una secuencia de ADN dúplex, en donde el oligonucleótido donador contiene por lo menos un nucleótido modificado que es una purina y/o pirimidina propinilada que tiene una afinidad de unión superior comparada con A, C, T o G que ocurren naturalmente y/o se une fuertemente a un nucleótido en una posición opuesta en la primera secuencia de ADN en comparación con un nucleótido que ocurre naturalmente complementario al nucleótido en la posición opuesta en la primera secuencia de ADN.
MX2008008258A 2005-12-22 2006-12-20 Intercambio de nucleotidos dirigido mejorado con oligonucleotidos modificados con propinilo. MX2008008258A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/NL2005/000884 WO2007073149A1 (en) 2005-12-22 2005-12-22 Alternative nucleotides for improved targeted nucleotide exchange
PCT/NL2006/000244 WO2007073154A1 (en) 2005-12-22 2006-05-09 Alternative nucleotides for improved targeted nucleotide exchange
PCT/NL2006/000649 WO2007073166A1 (en) 2005-12-22 2006-12-20 Improved targeted nucleotide exchange with propynyl modified oligonucleotides

Publications (1)

Publication Number Publication Date
MX2008008258A true MX2008008258A (es) 2008-09-24

Family

ID=36691461

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2008008258A MX2008008258A (es) 2005-12-22 2006-12-20 Intercambio de nucleotidos dirigido mejorado con oligonucleotidos modificados con propinilo.
MX2008008261A MX2008008261A (es) 2005-12-22 2006-12-21 Intercambio nucleotidico dirigido mejorado con oligonucleotidos modificados por lna.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2008008261A MX2008008261A (es) 2005-12-22 2006-12-21 Intercambio nucleotidico dirigido mejorado con oligonucleotidos modificados por lna.

Country Status (15)

Country Link
US (3) US20090307805A1 (es)
EP (4) EP1974053A1 (es)
JP (3) JP5295780B2 (es)
KR (2) KR20080092924A (es)
CN (3) CN101346474B (es)
AU (2) AU2006328050B2 (es)
BR (2) BRPI0621134A2 (es)
CA (2) CA2633741A1 (es)
ES (2) ES2393277T3 (es)
IL (2) IL192165A0 (es)
MX (2) MX2008008258A (es)
NZ (2) NZ569503A (es)
RU (2) RU2008130093A (es)
WO (4) WO2007073149A1 (es)
ZA (2) ZA200805091B (es)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007073149A1 (en) * 2005-12-22 2007-06-28 Keygene N.V. Alternative nucleotides for improved targeted nucleotide exchange
US20100223691A1 (en) * 2007-06-22 2010-09-02 Keygene N.V. Targeted nucleotide exchange with improved modified oligonucleotides
CN103146757B (zh) * 2007-12-21 2016-05-04 凯津公司 通过聚乙二醇的介导将致诱变核碱基引入植物原生质体的改进的诱变方法
KR101452818B1 (ko) * 2007-12-21 2014-10-23 키진 엔.브이. 식물 원형질체 내로 폴리에틸렌 글리콜 매개 돌연변이 뉴클레오염기의 도입을 이용한 개선된 돌연변이 생성방법
CN102257147A (zh) * 2008-12-22 2011-11-23 凯津公司 在植物原生质体中使用双链rna提高靶基因改变的效率
CA2951341A1 (en) * 2009-12-21 2011-06-30 Keygene N.V. Improved techniques for transfecting protoplasts
JP6084929B2 (ja) 2010-12-02 2017-02-22 キージーン・エン・フェー Ｄｎａの標的改変
US9518258B2 (en) 2010-12-02 2016-12-13 Keygene N.V. Targeted alteration of DNA with oligonucleotides
EP2702157A1 (en) 2011-04-29 2014-03-05 Keygene N.V. Glyphosate resistance enhancement
MX340053B (es) 2011-06-15 2016-06-22 Grifols Therapeutics Inc * Metodos, composiciones y kits para determinar el virus de la inmunodeficiencia humana (vih).
EP2554045A1 (en) 2011-08-04 2013-02-06 Rijk Zwaan Zaadteelt en Zaadhandel B.V. Method for systemically influencing processes in the male meiocyte
EP2581448B1 (en) * 2011-10-13 2015-01-28 Association Institut de Myologie Tricyclo-phosphorothioate DNA
CN102827251B (zh) * 2012-09-12 2014-06-18 中国人民解放军第四军医大学 一种透膜肽介导的反义抗菌剂及其制备方法和应用
US9622430B2 (en) 2012-09-28 2017-04-18 Nunhems B.V. Solanum lycopersicum plants having non-transgenic alterations in the ACS4 gene
CA2891720C (en) 2012-11-21 2022-05-03 Nunhems B.V. Solanum lycopersicum plants having non-transgenic alterations in the acs2 gene
WO2014118150A1 (en) 2013-01-31 2014-08-07 Nunhems B.V. Solanum lycopersicum plants having pink fruits
RS63427B1 (sr) 2013-03-15 2022-08-31 Cibus Us Llc Postupci i kompozicije za povećanje efikasnosti modifikacije ciljnog gena korišćenjem genske reparacije posredovane oligonukleotidom
US9957515B2 (en) 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
JP2013247961A (ja) * 2013-08-21 2013-12-12 Keygene Nv 植物細胞プロトプラストにおける二本鎖アクセプタｄｎａ配列の標的化改変の方法
KR102450868B1 (ko) 2014-03-14 2022-10-06 시버스 유에스 엘엘씨 올리고뉴클레오타이드 매개 유전자 보수를 사용한 표적화된 유전자 변형의 효율을 증가시키기 위한 방법 및 조성물
JP6997623B2 (ja) * 2014-12-12 2022-02-04 エム． ウルフ、トッド オリゴヌクレオチドを利用して細胞内の核酸を編集するための組成物および方法
WO2016105185A1 (en) 2014-12-22 2016-06-30 Keygene N.V. Plant callus populations
WO2016187284A1 (en) * 2015-05-19 2016-11-24 The University Of Chicago Methods and composition for determining ph
WO2017112941A1 (en) * 2015-12-23 2017-06-29 The Regents Of The University Of California Nano-sensors for nucleic acid detection and discrimination
CA3014036A1 (en) 2016-02-09 2017-08-17 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
EP3257944A1 (en) 2016-06-14 2017-12-20 Nunhems B.V. Tomato plants having alterations in the dmr6 gene
WO2018115389A1 (en) 2016-12-22 2018-06-28 Keygene N.V. Methods of targeted genetic alteration in plant cells
AU2019207703A1 (en) 2018-01-09 2020-07-16 Cibus Europe B.V. Shatterproof genes and mutations
NL2020823B1 (en) 2018-04-25 2019-11-05 Univ Delft Tech NAD+ dependent 7ß-hydroxysteroid dehydrogenase
EP3363751B1 (de) 2018-06-05 2020-04-22 B&R Industrial Automation GmbH Verfahren zur übergabe einer transporteinheit eines langstatorlinearmotors an einer übergabeposition
US20210238612A1 (en) 2018-07-12 2021-08-05 Keygene N.V. Type v crispr/nuclease-system for genome editing in plant cells
JP2022505440A (ja) 2018-11-01 2022-01-14 キージーン ナムローゼ フェンノートシャップ 植物細胞におけるＣＲＩＳＰＲ／Ｃａｓゲノム編集のためのデュアルガイドＲＮＡ
CN114262735A (zh) * 2021-12-15 2022-04-01 成都齐碳科技有限公司 用于表征多核苷酸的衔接体及其用途
WO2023168273A2 (en) * 2022-03-01 2023-09-07 Colorado State University Research Foundation Targeted control of weeds and invasive plants by delivery of fana antisense oligonucleotides

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US645249A (en) * 1899-06-10 1900-03-13 Us Fire And Police Telegraph Company Bell-striker.
US5849482A (en) * 1988-09-28 1998-12-15 Epoch Pharmaceuticals, Inc. Crosslinking oligonucleotides
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
MX9207334A (es) 1991-12-18 1993-08-01 Glaxo Inc Acidos nucleicos peptidicos y formulacion farma- ceutica que los contiene
US5731181A (en) * 1996-06-17 1998-03-24 Thomas Jefferson University Chimeric mutational vectors having non-natural nucleotides
ATE232880T1 (de) 1996-11-18 2003-03-15 Takeshi Imanishi Neue nucleotidanaloga
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
US6455249B1 (en) * 1997-09-10 2002-09-24 National Institutes Of Health Method of amplifying DNA and RNA mismatch cleavage products
EP2253639A1 (en) 1997-09-12 2010-11-24 Exiqon A/S Bi- and tri-cyclic nucleoside, nucleotide and oligonucleoide analogues
US6010907A (en) 1998-05-12 2000-01-04 Kimeragen, Inc. Eukaryotic use of non-chimeric mutational vectors
ES2268903T3 (es) * 1998-10-26 2007-03-16 Avi Biopharma, Inc. Agente antisentido de p53 y procedimiento.
WO2000056748A1 (en) 1999-03-18 2000-09-28 Exiqon A/S Xylo-lna analogues
EP1178999B1 (en) 1999-05-04 2007-03-14 Santaris Pharma A/S L-ribo-lna analogues
EP1244667B1 (en) * 1999-12-30 2006-04-05 K.U. Leuven Research & Development Cyclohexene nucleic acids
CA2404780A1 (en) * 2000-03-27 2001-10-04 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides
US6936467B2 (en) 2000-03-27 2005-08-30 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides
EP1152058A1 (en) * 2000-05-03 2001-11-07 Institut Curie Methods and compositions for effecting homologous recombination
CA2409172A1 (en) 2000-05-17 2001-11-22 University Of Delaware Plant gene targeting using oligonucleotides
HUP0301833A3 (en) * 2000-07-19 2005-12-28 Bristol Myers Squibb Pharma Co Crf2 ligands in combination therapy
AU2001279069A1 (en) 2000-07-27 2002-02-13 University Of Delaware Methods for enhancing targeted gene alteration using oligonucleotides
US20020119570A1 (en) * 2000-09-25 2002-08-29 Kyonggeun Yoon Targeted gene correction by single-stranded oligodeoxynucleotides
US20050148530A1 (en) * 2002-02-20 2005-07-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
WO2003027265A2 (en) 2001-09-27 2003-04-03 University Of Delaware Composition and methods for enhancing oligonucleotide-directed nucleic acid sequence alteration
SI2264172T1 (sl) * 2002-04-05 2017-12-29 Roche Innovation Center Copenhagen A/S Oligomerne spojine za modulacijo izražanja HIF-1alfa
AU2003251986A1 (en) * 2002-07-17 2004-02-02 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
EP3502252B1 (en) * 2003-06-02 2023-04-05 University of Massachusetts Methods and compositions for controlling efficacy of rna silencing
CA2546067A1 (en) * 2003-11-14 2005-06-02 Yale University Fcyriia-specific nucleic acid interference
WO2007073149A1 (en) * 2005-12-22 2007-06-28 Keygene N.V. Alternative nucleotides for improved targeted nucleotide exchange

Also Published As

Publication number Publication date
AU2006328050B2 (en) 2012-12-06
JP2009520495A (ja) 2009-05-28
AU2006328050A1 (en) 2007-06-28
WO2007073170A1 (en) 2007-06-28
EP2002001B1 (en) 2013-03-13
JP5295780B2 (ja) 2013-09-18
ZA200805141B (en) 2009-09-30
RU2463350C2 (ru) 2012-10-10
ES2407679T3 (es) 2013-06-13
EP1963505B1 (en) 2012-08-22
RU2008130089A (ru) 2010-01-27
JP2009520498A (ja) 2009-05-28
JP5340742B2 (ja) 2013-11-13
WO2007073149A1 (en) 2007-06-28
EP2333115A1 (en) 2011-06-15
JP5405121B2 (ja) 2014-02-05
CA2633640A1 (en) 2007-06-28
AU2006328054A1 (en) 2007-06-28
MX2008008261A (es) 2008-09-24
RU2008130093A (ru) 2010-01-27
CN101346474A (zh) 2009-01-14
EP1974053A1 (en) 2008-10-01
US20100055780A1 (en) 2010-03-04
NZ569503A (en) 2011-12-22
CN101346466B (zh) 2013-06-12
EP1963505A1 (en) 2008-09-03
IL192164A0 (en) 2008-12-29
US20100186124A1 (en) 2010-07-22
BRPI0621134A2 (pt) 2011-11-29
KR20080092924A (ko) 2008-10-16
IL192165A0 (en) 2008-12-29
CN101346465A (zh) 2009-01-14
JP2009520499A (ja) 2009-05-28
CA2633741A1 (en) 2007-06-28
CN101346466A (zh) 2009-01-14
AU2006328054B2 (en) 2012-12-13
ES2393277T3 (es) 2012-12-20
US20090307805A1 (en) 2009-12-10
WO2007073154A1 (en) 2007-06-28
EP2002001A1 (en) 2008-12-17
BRPI0621133A2 (pt) 2011-11-29
CN101346474B (zh) 2013-06-26
NZ569502A (en) 2011-12-22
WO2007073166A1 (en) 2007-06-28
CN101346465B (zh) 2013-06-12
KR20080083179A (ko) 2008-09-16
ZA200805091B (en) 2009-06-24

Similar Documents

Publication Publication Date Title
MX2008008258A (es) Intercambio de nucleotidos dirigido mejorado con oligonucleotidos modificados con propinilo.
WO2006125977A3 (en) Hybrid interfering rna
WO2009135093A3 (en) Rnase-h-based assays utilizing modified rna monomers
WO2005120230A3 (en) POSITIONALLY MODIFIED siRNA CONSTRUCTS
WO2007085485A3 (en) Lna modified phosphorothiolated oligonucleotides
BRPI0813342A2 (pt) " composição de dna polimerase termoestável, e, métodos para estabilizar e ou realçar a atividade de dna polimerase e para realizar pcr ".
EP1845160A4 (en) NUCLEOTIDE-amplification
WO2006126040A8 (en) Bacterial and bacterial associated mirnas and uses thereof
WO2008150729A3 (en) N-substituted-aminomethylene bridged bicyclic nucleic acid analogs
WO2009023855A3 (en) Tetrahydropyran nucleic acid analogs
WO2007082881A3 (en) Hybridization probe assay and array
GB0514910D0 (en) Method for sequencing a polynucleotide template
IL202530A0 (en) Targeted nucleotide exchange with improved modified oligonucleotides
WO2009102896A3 (en) Isothermal nucleic acid amplification methods and compositions
WO2005079397A3 (en) Anti-microrna oligonucleotide molecules
WO2008051530A3 (en) Polymerase enzymes and reagents for enhanced nucleic acid sequencing
WO2007118138A3 (en) Validation of comparative genomic hybridization
WO2008061537A3 (en) Oligonucleotides for modulating target rna activity
MX2010012849A (es) Reactivo de lisis, enlace y/o lavado para aislar y/o purificar acidos nucleicos.
WO2007128982A3 (en) Compositions and methods for epigenetic modification of nucleic acid sequences in vivo
WO2009014612A3 (en) Modified nucleotides, methods for making and using same
WO2008140829A3 (en) Multiplex detection of agricultural pathogens
WO2007048244A3 (en) Small interfering ribonucleic acid duplexes comprising arabinose modified nucleotides
EP2157182A3 (en) Modified short interfering RNA
GB0621361D0 (en) Use of DNA polymerases

Legal Events

Date Code Title Description
FG Grant or registration