BRPI0813342A2 - " composição de dna polimerase termoestável, e, métodos para estabilizar e ou realçar a atividade de dna polimerase e para realizar pcr ". - Google Patents
" composição de dna polimerase termoestável, e, métodos para estabilizar e ou realçar a atividade de dna polimerase e para realizar pcr ".Info
- Publication number
- BRPI0813342A2 BRPI0813342A2 BRPI0813342-5A2A BRPI0813342A BRPI0813342A2 BR PI0813342 A2 BRPI0813342 A2 BR PI0813342A2 BR PI0813342 A BRPI0813342 A BR PI0813342A BR PI0813342 A2 BRPI0813342 A2 BR PI0813342A2
- Authority
- BR
- Brazil
- Prior art keywords
- dna polymerase
- stabilizing
- methods
- performing pcr
- enhancing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US94366707P | 2007-06-13 | 2007-06-13 | |
PCT/EP2008/057404 WO2008152102A1 (en) | 2007-06-13 | 2008-06-12 | Polymerase stabilization |
Publications (1)
Publication Number | Publication Date |
---|---|
BRPI0813342A2 true BRPI0813342A2 (pt) | 2014-10-14 |
Family
ID=39677430
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0813342-5A2A BRPI0813342A2 (pt) | 2007-06-13 | 2008-06-12 | " composição de dna polimerase termoestável, e, métodos para estabilizar e ou realçar a atividade de dna polimerase e para realizar pcr ". |
Country Status (8)
Country | Link |
---|---|
US (1) | US20100159528A1 (pt) |
EP (1) | EP2152864A1 (pt) |
JP (1) | JP2010528669A (pt) |
CN (1) | CN101679957A (pt) |
AU (1) | AU2008263931A1 (pt) |
BR (1) | BRPI0813342A2 (pt) |
CA (1) | CA2689068A1 (pt) |
WO (1) | WO2008152102A1 (pt) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7846703B2 (en) * | 2006-10-02 | 2010-12-07 | Takara Bio Inc. | Method for enhancing polymerase activity |
US20110250598A1 (en) * | 2010-04-12 | 2011-10-13 | Ulrike Fischer | Detergent free polymerases |
US9845489B2 (en) | 2010-07-26 | 2017-12-19 | Biomatrica, Inc. | Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures |
US9376709B2 (en) | 2010-07-26 | 2016-06-28 | Biomatrica, Inc. | Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures |
US8715987B2 (en) | 2011-05-02 | 2014-05-06 | New England Biolabs, Inc. | Solubilized phospholipids for stabilizing nucleic acid polymerases |
US9567628B2 (en) | 2011-06-08 | 2017-02-14 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
CN106518696B (zh) | 2011-06-08 | 2019-06-14 | 生命技术公司 | 用于pcr系统的新型去污剂的设计和开发 |
CN113388606A (zh) | 2011-09-26 | 2021-09-14 | 凯杰有限公司 | 用于分离细胞外核酸的快速方法 |
JP6076138B2 (ja) * | 2012-03-02 | 2017-02-08 | ローム アンド ハース エレクトロニック マテリアルズ エルエルシーRohm and Haas Electronic Materials LLC | カーボンブラックと金属との複合体 |
US8956816B2 (en) | 2012-06-05 | 2015-02-17 | Pacific Biosciences Of California, Inc. | Methods and compositions for performing analytical operations |
US9085761B1 (en) | 2012-06-14 | 2015-07-21 | Affymetrix, Inc. | Methods and compositions for amplification of nucleic acids |
JP6433075B2 (ja) * | 2012-11-07 | 2018-12-05 | キアゲン ゲーエムベーハー | 診断アッセイのための制御 |
US9725703B2 (en) | 2012-12-20 | 2017-08-08 | Biomatrica, Inc. | Formulations and methods for stabilizing PCR reagents |
CN104560946A (zh) * | 2013-10-10 | 2015-04-29 | 镇江拜因诺生物科技有限公司 | 甘油糖基甘油酸作为增效剂在pcr中的应用 |
JP6742238B2 (ja) | 2013-10-25 | 2020-08-19 | ライフ テクノロジーズ コーポレーション | Pcrシステムにおいて使用するための新規化合物及びその用途 |
CN106572650B (zh) | 2014-06-10 | 2021-08-31 | 生物马特里卡公司 | 在环境温度下稳定凝血细胞 |
CN107075544B (zh) | 2014-07-22 | 2021-01-29 | 生物辐射实验室股份有限公司 | 与聚合酶联用的缓冲液 |
WO2016198571A1 (en) | 2015-06-10 | 2016-12-15 | Qiagen Gmbh | Method for isolating extracellular nucleic acids using anion exchange particles |
EP4242628A3 (en) | 2015-12-08 | 2023-11-08 | Biomatrica, INC. | Reduction of erythrocyte sedimentation rate |
WO2018175399A1 (en) | 2017-03-24 | 2018-09-27 | Bio-Rad Laboratories, Inc. | Universal hairpin primers |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4758580A (en) * | 1985-06-26 | 1988-07-19 | Yamanouchi Pharmaceutical Co., Ltd. | Inhibiting growth of tumors with certain substituted phenoxy dimethyl alkanoic acids, esters or salts |
US6127155A (en) * | 1986-08-22 | 2000-10-03 | Roche Molecular Systems, Inc. | Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents |
US5405774A (en) * | 1986-08-22 | 1995-04-11 | Hoffmann-La Roche Inc. | DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 |
JP2531246B2 (ja) * | 1988-08-26 | 1996-09-04 | 東洋紡績株式会社 | 耐熱性dnaポリメラ―ゼおよびその製法 |
US5242818A (en) * | 1988-08-26 | 1993-09-07 | Toyo Boseki Kabushiki Kaisha | Method of producing a thermostable DNA polymerase from Thermus thermophilus |
US5322785A (en) * | 1990-04-26 | 1994-06-21 | New England Biolabs, Inc. | Purified thermostable DNA polymerase obtainable from thermococcus litoralis |
US5948663A (en) * | 1990-12-03 | 1999-09-07 | Stratagene | Purified thermostable pyrococcus furiosus DNA polymerase I |
CA2184270C (en) * | 1994-03-10 | 1999-08-17 | Daniel Louis Kacian | Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents |
US5622822A (en) * | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
CN1123328A (zh) * | 1994-11-17 | 1996-05-29 | 中国科学院上海生物化学研究所 | 高温、高传真脱氧核糖核酸(DNA)聚合酶(HiFi Bst) |
US5602011A (en) * | 1995-01-18 | 1997-02-11 | Pharmacia Biotech Inc. | Purified Thermococcus barossii DNA polymerase |
US6033859A (en) * | 1996-05-24 | 2000-03-07 | Toyo Boseki Kabushiki Kaisha | Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1 |
US5861295A (en) * | 1997-01-02 | 1999-01-19 | Life Technologies, Inc. | Nucleic acid-free thermostable enzymes and methods of production thereof |
US5882904A (en) * | 1997-08-04 | 1999-03-16 | Amersham Pharmacia Biotech Inc. | Thermococcus barossii DNA polymerase mutants |
US6242235B1 (en) * | 1998-06-24 | 2001-06-05 | Promega Corp. | Polymerase stabilization by polyethoxylated amine surfactants |
US20030134292A1 (en) * | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
US7972828B2 (en) * | 2006-12-19 | 2011-07-05 | Sigma-Aldrich Co. | Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent |
-
2008
- 2008-06-12 US US12/601,102 patent/US20100159528A1/en not_active Abandoned
- 2008-06-12 WO PCT/EP2008/057404 patent/WO2008152102A1/en active Application Filing
- 2008-06-12 AU AU2008263931A patent/AU2008263931A1/en not_active Abandoned
- 2008-06-12 CA CA002689068A patent/CA2689068A1/en not_active Abandoned
- 2008-06-12 CN CN200880019744A patent/CN101679957A/zh active Pending
- 2008-06-12 BR BRPI0813342-5A2A patent/BRPI0813342A2/pt not_active IP Right Cessation
- 2008-06-12 EP EP08760944A patent/EP2152864A1/en not_active Withdrawn
- 2008-06-12 JP JP2010511644A patent/JP2010528669A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
CA2689068A1 (en) | 2008-12-18 |
JP2010528669A (ja) | 2010-08-26 |
EP2152864A1 (en) | 2010-02-17 |
WO2008152102A1 (en) | 2008-12-18 |
AU2008263931A1 (en) | 2008-12-18 |
US20100159528A1 (en) | 2010-06-24 |
CN101679957A (zh) | 2010-03-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE AS 6A E 7A ANUIDADES. |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2329 DE 25-08-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |
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B350 | Update of information on the portal [chapter 15.35 patent gazette] |