ZA200805091B - Improved targeted nucleotide exchange with LNA modified oligonucleotides - Google Patents

Improved targeted nucleotide exchange with LNA modified oligonucleotides

Info

Publication number
ZA200805091B
ZA200805091B ZA200805091A ZA200805091A ZA200805091B ZA 200805091 B ZA200805091 B ZA 200805091B ZA 200805091 A ZA200805091 A ZA 200805091A ZA 200805091 A ZA200805091 A ZA 200805091A ZA 200805091 B ZA200805091 B ZA 200805091B
Authority
ZA
South Africa
Prior art keywords
modified oligonucleotides
nucleotide exchange
lna modified
targeted nucleotide
improved targeted
Prior art date
Application number
ZA200805091A
Other languages
English (en)
Inventor
Bundock Paul
De Both Michiel Theodoor Jan
Hogers Rene Cornelis Josephus
Wachowski Ludvik Kevin
Original Assignee
Keygene Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36691461&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ZA200805091(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Keygene Nv filed Critical Keygene Nv
Publication of ZA200805091B publication Critical patent/ZA200805091B/xx

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278—Sulfonylurea
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
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  • Biochemistry (AREA)
  • Microbiology (AREA)
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  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
ZA200805091A 2005-12-22 2008-06-11 Improved targeted nucleotide exchange with LNA modified oligonucleotides ZA200805091B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/NL2005/000884 WO2007073149A1 (en) 2005-12-22 2005-12-22 Alternative nucleotides for improved targeted nucleotide exchange

Publications (1)

Publication Number Publication Date
ZA200805091B true ZA200805091B (en) 2009-06-24

Family

ID=36691461

Family Applications (2)

Application Number Title Priority Date Filing Date
ZA200805091A ZA200805091B (en) 2005-12-22 2008-06-11 Improved targeted nucleotide exchange with LNA modified oligonucleotides
ZA200805141A ZA200805141B (en) 2005-12-22 2008-06-12 Improved targeted nucleotide exchange with propynyl modified oligonucleotides

Family Applications After (1)

Application Number Title Priority Date Filing Date
ZA200805141A ZA200805141B (en) 2005-12-22 2008-06-12 Improved targeted nucleotide exchange with propynyl modified oligonucleotides

Country Status (15)

Country Link
US (3) US20090307805A1 (xx)
EP (4) EP1974053A1 (xx)
JP (3) JP5295780B2 (xx)
KR (2) KR20080092924A (xx)
CN (3) CN101346474B (xx)
AU (2) AU2006328050B2 (xx)
BR (2) BRPI0621134A2 (xx)
CA (2) CA2633741A1 (xx)
ES (2) ES2393277T3 (xx)
IL (2) IL192164A0 (xx)
MX (2) MX2008008258A (xx)
NZ (2) NZ569503A (xx)
RU (2) RU2008130093A (xx)
WO (4) WO2007073149A1 (xx)
ZA (2) ZA200805091B (xx)

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WO2010074562A1 (en) 2008-12-22 2010-07-01 Keygene N.V. Use of double stranded rna to increase the efficiency of targeted gene alteration in plant protoplasts
CN102791865B (zh) 2009-12-21 2015-07-01 凯津公司 用于转染原生质体的改进技术
JP6084929B2 (ja) * 2010-12-02 2017-02-22 キージーン・エン・フェー Ｄｎａの標的改変
WO2012074386A1 (en) 2010-12-02 2012-06-07 Keygene N.V. Targeted alteration of dna with oligonucleotides
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US11136618B2 (en) * 2015-05-19 2021-10-05 The University Of Chicago Methods and compositions for determining pH
WO2017112941A1 (en) * 2015-12-23 2017-06-29 The Regents Of The University Of California Nano-sensors for nucleic acid detection and discrimination
US11542515B2 (en) 2016-02-09 2023-01-03 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
EP3257944A1 (en) 2016-06-14 2017-12-20 Nunhems B.V. Tomato plants having alterations in the dmr6 gene
WO2018115389A1 (en) 2016-12-22 2018-06-28 Keygene N.V. Methods of targeted genetic alteration in plant cells
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US20100186124A1 (en) 2010-07-22
EP1974053A1 (en) 2008-10-01
JP2009520495A (ja) 2009-05-28
AU2006328054B2 (en) 2012-12-13
EP2333115A1 (en) 2011-06-15
KR20080083179A (ko) 2008-09-16
MX2008008261A (es) 2008-09-24
MX2008008258A (es) 2008-09-24
CN101346465A (zh) 2009-01-14
WO2007073166A1 (en) 2007-06-28
EP2002001A1 (en) 2008-12-17
KR20080092924A (ko) 2008-10-16
WO2007073154A1 (en) 2007-06-28
WO2007073170A1 (en) 2007-06-28
CN101346474A (zh) 2009-01-14
JP5340742B2 (ja) 2013-11-13
WO2007073149A1 (en) 2007-06-28
NZ569503A (en) 2011-12-22
AU2006328050A1 (en) 2007-06-28
ES2407679T3 (es) 2013-06-13
JP5405121B2 (ja) 2014-02-05
US20090307805A1 (en) 2009-12-10
BRPI0621133A2 (pt) 2011-11-29
IL192165A0 (en) 2008-12-29
US20100055780A1 (en) 2010-03-04
CA2633640A1 (en) 2007-06-28
CN101346466B (zh) 2013-06-12
ZA200805141B (en) 2009-09-30
AU2006328054A1 (en) 2007-06-28
JP5295780B2 (ja) 2013-09-18
CA2633741A1 (en) 2007-06-28
CN101346474B (zh) 2013-06-26
EP1963505A1 (en) 2008-09-03
ES2393277T3 (es) 2012-12-20
IL192164A0 (en) 2008-12-29
BRPI0621134A2 (pt) 2011-11-29
RU2008130093A (ru) 2010-01-27
JP2009520498A (ja) 2009-05-28
RU2463350C2 (ru) 2012-10-10
RU2008130089A (ru) 2010-01-27
AU2006328050B2 (en) 2012-12-06
NZ569502A (en) 2011-12-22
EP2002001B1 (en) 2013-03-13
CN101346465B (zh) 2013-06-12
EP1963505B1 (en) 2012-08-22
JP2009520499A (ja) 2009-05-28
CN101346466A (zh) 2009-01-14

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