MX168908B - Metodo para regular la expresion de un gen exterior controlando la temperatura de cultivo y el procedimiento para producir un producto de gen exterior - Google Patents
Metodo para regular la expresion de un gen exterior controlando la temperatura de cultivo y el procedimiento para producir un producto de gen exteriorInfo
- Publication number
- MX168908B MX168908B MX010485A MX1048588A MX168908B MX 168908 B MX168908 B MX 168908B MX 010485 A MX010485 A MX 010485A MX 1048588 A MX1048588 A MX 1048588A MX 168908 B MX168908 B MX 168908B
- Authority
- MX
- Mexico
- Prior art keywords
- expression
- external gene
- regulating
- procedure
- producing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
La presente invención se refiere a un método para regular la expresión de un gen exterior en una bacteria de Escherichia coli que lleva un plásmido recombinante construído insertando un gen exterior en un vector de expresión para permitir la expresión del gen exterior, que comprende un procedimiento de mantener la temperatura del cultivo de la E. coli a 40 grados C o más de manera que se suprima la expresión del gen exterior.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62034397A JPS63202386A (ja) | 1987-02-19 | 1987-02-19 | 外来遺伝子の発現制御方法及び該方法を用いた外来遺伝子産物の生産方法 |
JP62152359A JPH0817704B2 (ja) | 1987-06-18 | 1987-06-18 | 外来遺伝子産物の生産方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
MX168908B true MX168908B (es) | 1993-06-14 |
Family
ID=26373202
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX010485A MX168908B (es) | 1987-02-19 | 1988-02-19 | Metodo para regular la expresion de un gen exterior controlando la temperatura de cultivo y el procedimiento para producir un producto de gen exterior |
Country Status (8)
Country | Link |
---|---|
US (2) | US5043277A (es) |
EP (1) | EP0279665B1 (es) |
KR (1) | KR910001812B1 (es) |
CA (1) | CA1320922C (es) |
DE (1) | DE3877012T2 (es) |
DK (1) | DK88488A (es) |
ES (1) | ES2043805T3 (es) |
MX (1) | MX168908B (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0462836A3 (en) * | 1990-06-20 | 1992-08-26 | Mitsui Toatsu Chemicals, Inc. | Recombinant vector plasmid capable of expressing human manganese superoxide dismutase, and process of producing this enzyme |
ATE195142T1 (de) * | 1991-02-26 | 2000-08-15 | Zeneca Ltd | Fermentationsverfahren zur herstellung von peptiden durch einen wirt |
SE9401921D0 (sv) * | 1994-06-03 | 1994-06-03 | Sbl Vaccin Ab | A method of cultivating bacteria |
US6521748B2 (en) | 1999-08-06 | 2003-02-18 | E. I. Du Pont De Nemours And Company | Polynucleotide encoding a mutant Rhodotorula glutinis tyrosine ammonia lyase polypeptide |
US6368837B1 (en) * | 1999-08-06 | 2002-04-09 | E. I. Du Pont Nemours And Company | Bioproduction of para-hydroxycinnamic acid |
EP1400593A4 (en) * | 2001-06-06 | 2005-04-06 | Takeda Pharmaceutical | NEW PROMOTER |
EP4015787B1 (en) | 2020-12-17 | 2024-01-24 | C.R.F. Società Consortile per Azioni | Internal combustion engine with fast combustion, and method for controlling the engine |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60221091A (ja) * | 1983-12-21 | 1985-11-05 | Kyowa Hakko Kogyo Co Ltd | 新規プロモ−タ− |
GB8621626D0 (en) * | 1986-09-08 | 1986-10-15 | Health Lab Service Board | Phenylalanine ammonia lyase |
JP2507345B2 (ja) * | 1986-09-16 | 1996-06-12 | 三井東圧化学株式会社 | L―フェニルアラニン―アンモニアリ・ア―ゼ構造遺伝子 |
-
1988
- 1988-02-17 CA CA000559127A patent/CA1320922C/en not_active Expired - Fee Related
- 1988-02-17 US US07/156,814 patent/US5043277A/en not_active Expired - Lifetime
- 1988-02-18 DE DE8888301356T patent/DE3877012T2/de not_active Expired - Fee Related
- 1988-02-18 ES ES88301356T patent/ES2043805T3/es not_active Expired - Lifetime
- 1988-02-18 EP EP88301356A patent/EP0279665B1/en not_active Expired - Lifetime
- 1988-02-19 KR KR1019880001742A patent/KR910001812B1/ko not_active IP Right Cessation
- 1988-02-19 MX MX010485A patent/MX168908B/es unknown
- 1988-02-19 DK DK088488A patent/DK88488A/da not_active Application Discontinuation
-
1991
- 1991-11-27 US US07/798,044 patent/US5322786A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
KR910001812B1 (ko) | 1991-03-26 |
DK88488D0 (da) | 1988-02-19 |
US5043277A (en) | 1991-08-27 |
DE3877012T2 (de) | 1993-04-15 |
CA1320922C (en) | 1993-08-03 |
EP0279665A3 (en) | 1989-06-14 |
KR880010129A (ko) | 1988-10-07 |
US5322786A (en) | 1994-06-21 |
EP0279665A2 (en) | 1988-08-24 |
DK88488A (da) | 1988-08-20 |
ES2043805T3 (es) | 1994-01-01 |
DE3877012D1 (de) | 1993-02-11 |
EP0279665B1 (en) | 1992-12-30 |
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