MX168908B - Metodo para regular la expresion de un gen exterior controlando la temperatura de cultivo y el procedimiento para producir un producto de gen exterior - Google Patents

Metodo para regular la expresion de un gen exterior controlando la temperatura de cultivo y el procedimiento para producir un producto de gen exterior

Info

Publication number
MX168908B
MX168908B MX010485A MX1048588A MX168908B MX 168908 B MX168908 B MX 168908B MX 010485 A MX010485 A MX 010485A MX 1048588 A MX1048588 A MX 1048588A MX 168908 B MX168908 B MX 168908B
Authority
MX
Mexico
Prior art keywords
expression
external gene
regulating
procedure
producing
Prior art date
Application number
MX010485A
Other languages
English (en)
Inventor
Naki Suzuki
Yoshiyuki Nakajima
Nobuhiro Fukuhara
Kaoru Yamamoto
Satori Sone
Original Assignee
Mitsuit Toatsu Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP62034397A external-priority patent/JPS63202386A/ja
Priority claimed from JP62152359A external-priority patent/JPH0817704B2/ja
Application filed by Mitsuit Toatsu Chemicals Inc filed Critical Mitsuit Toatsu Chemicals Inc
Publication of MX168908B publication Critical patent/MX168908B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente invención se refiere a un método para regular la expresión de un gen exterior en una bacteria de Escherichia coli que lleva un plásmido recombinante construído insertando un gen exterior en un vector de expresión para permitir la expresión del gen exterior, que comprende un procedimiento de mantener la temperatura del cultivo de la E. coli a 40 grados C o más de manera que se suprima la expresión del gen exterior.
MX010485A 1987-02-19 1988-02-19 Metodo para regular la expresion de un gen exterior controlando la temperatura de cultivo y el procedimiento para producir un producto de gen exterior MX168908B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP62034397A JPS63202386A (ja) 1987-02-19 1987-02-19 外来遺伝子の発現制御方法及び該方法を用いた外来遺伝子産物の生産方法
JP62152359A JPH0817704B2 (ja) 1987-06-18 1987-06-18 外来遺伝子産物の生産方法

Publications (1)

Publication Number Publication Date
MX168908B true MX168908B (es) 1993-06-14

Family

ID=26373202

Family Applications (1)

Application Number Title Priority Date Filing Date
MX010485A MX168908B (es) 1987-02-19 1988-02-19 Metodo para regular la expresion de un gen exterior controlando la temperatura de cultivo y el procedimiento para producir un producto de gen exterior

Country Status (8)

Country Link
US (2) US5043277A (es)
EP (1) EP0279665B1 (es)
KR (1) KR910001812B1 (es)
CA (1) CA1320922C (es)
DE (1) DE3877012T2 (es)
DK (1) DK88488A (es)
ES (1) ES2043805T3 (es)
MX (1) MX168908B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0462836A3 (en) * 1990-06-20 1992-08-26 Mitsui Toatsu Chemicals, Inc. Recombinant vector plasmid capable of expressing human manganese superoxide dismutase, and process of producing this enzyme
ATE195142T1 (de) * 1991-02-26 2000-08-15 Zeneca Ltd Fermentationsverfahren zur herstellung von peptiden durch einen wirt
SE9401921D0 (sv) * 1994-06-03 1994-06-03 Sbl Vaccin Ab A method of cultivating bacteria
US6368837B1 (en) 1999-08-06 2002-04-09 E. I. Du Pont Nemours And Company Bioproduction of para-hydroxycinnamic acid
US6521748B2 (en) 1999-08-06 2003-02-18 E. I. Du Pont De Nemours And Company Polynucleotide encoding a mutant Rhodotorula glutinis tyrosine ammonia lyase polypeptide
US20040166566A1 (en) * 2001-06-06 2004-08-26 Takashi Ito Novel promoter
EP4015787B1 (en) 2020-12-17 2024-01-24 C.R.F. Società Consortile per Azioni Internal combustion engine with fast combustion, and method for controlling the engine

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60221091A (ja) * 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
GB8621626D0 (en) * 1986-09-08 1986-10-15 Health Lab Service Board Phenylalanine ammonia lyase
JP2507345B2 (ja) * 1986-09-16 1996-06-12 三井東圧化学株式会社 Ｌ―フェニルアラニン―アンモニアリ・ア―ゼ構造遺伝子

Also Published As

Publication number Publication date
DK88488A (da) 1988-08-20
EP0279665A2 (en) 1988-08-24
EP0279665A3 (en) 1989-06-14
CA1320922C (en) 1993-08-03
KR880010129A (ko) 1988-10-07
KR910001812B1 (ko) 1991-03-26
ES2043805T3 (es) 1994-01-01
US5043277A (en) 1991-08-27
DE3877012T2 (de) 1993-04-15
DK88488D0 (da) 1988-02-19
US5322786A (en) 1994-06-21
EP0279665B1 (en) 1992-12-30
DE3877012D1 (de) 1993-02-11

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