ES2043805T3 - Un metodo para regular la expresion de un gen extra\o controlando la temperatura del cultivo y procedimiento para producir un producto de gen extra\o por este metodo. - Google Patents

Un metodo para regular la expresion de un gen extra\o controlando la temperatura del cultivo y procedimiento para producir un producto de gen extra\o por este metodo.

Info

Publication number
ES2043805T3
ES2043805T3 ES88301356T ES88301356T ES2043805T3 ES 2043805 T3 ES2043805 T3 ES 2043805T3 ES 88301356 T ES88301356 T ES 88301356T ES 88301356 T ES88301356 T ES 88301356T ES 2043805 T3 ES2043805 T3 ES 2043805T3
Authority
ES
Spain
Prior art keywords
expression
extra gene
regulating
procedure
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88301356T
Other languages
English (en)
Inventor
Nobuhiro Fukuhara
Setsuo Yoshino
Kaoru Yamamoto
Satori Sone
Maki Suzuki
Yoshiyuki Nakajima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Toatsu Chemicals Inc
Original Assignee
Mitsui Toatsu Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP62034397A external-priority patent/JPS63202386A/ja
Priority claimed from JP62152359A external-priority patent/JPH0817704B2/ja
Application filed by Mitsui Toatsu Chemicals Inc filed Critical Mitsui Toatsu Chemicals Inc
Application granted granted Critical
Publication of ES2043805T3 publication Critical patent/ES2043805T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

ESCHERISCHIA COLI, QUE PORTA UN PLASMIDO HIBRIDO, QUE HA SIDO CONSTRUIDO POR INSERCION DE UN GEN EXTRAÑO DESEADO EN UN VECTOR DE EXPRESION, DE MANERA QUE PERMITE LA EXPRESION DE DICHO GEN EXTRAÑO EN EL, SE CULTIVA A UNA TEMPERATURA DE 40 (GRADOS) C O SUPERIOR, DE MODO QUE LA EXPRESION DE DICHO GEN EXTRAÑO SE DETIENE. ESTE E. COLI (POR EJEMPLO, TRANSFORMANTE) SE CULTIVA A 40 (GRADOS) C O MAS EN UN PRIMER PROCESO PARA DETENER LA EXPRESION DEL GEN EXTRAÑO Y MANTENER SUFICIENTE CRECIMIENTO CELULAR, Y DESPUES POR DEBAJO DE 40 (GRADOS) C EN UN SEGUNDO PROCESO PARA QUITAR LA SUPRESION DE LA EXPRESION, DE MODO QUE PERMITA LA PRODUCCION EFECTIVA DEL PRODUCTO CON GEN EXTRAÑO, LO QUE DA LUGAR A UNA ALTA CONCENTRACION DEL PRODUCTO CON GEN EXTRAÑO EN EL CULTIVO FINAL.
ES88301356T 1987-02-19 1988-02-18 Un metodo para regular la expresion de un gen extra\o controlando la temperatura del cultivo y procedimiento para producir un producto de gen extra\o por este metodo. Expired - Lifetime ES2043805T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP62034397A JPS63202386A (ja) 1987-02-19 1987-02-19 外来遺伝子の発現制御方法及び該方法を用いた外来遺伝子産物の生産方法
JP62152359A JPH0817704B2 (ja) 1987-06-18 1987-06-18 外来遺伝子産物の生産方法

Publications (1)

Publication Number Publication Date
ES2043805T3 true ES2043805T3 (es) 1994-01-01

Family

ID=26373202

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88301356T Expired - Lifetime ES2043805T3 (es) 1987-02-19 1988-02-18 Un metodo para regular la expresion de un gen extra\o controlando la temperatura del cultivo y procedimiento para producir un producto de gen extra\o por este metodo.

Country Status (8)

Country Link
US (2) US5043277A (es)
EP (1) EP0279665B1 (es)
KR (1) KR910001812B1 (es)
CA (1) CA1320922C (es)
DE (1) DE3877012T2 (es)
DK (1) DK88488A (es)
ES (1) ES2043805T3 (es)
MX (1) MX168908B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0462836A3 (en) * 1990-06-20 1992-08-26 Mitsui Toatsu Chemicals, Inc. Recombinant vector plasmid capable of expressing human manganese superoxide dismutase, and process of producing this enzyme
DE69231306T2 (de) * 1991-02-26 2001-01-11 Zeneca Ltd., London Fermentationsverfahren zur Herstellung von Peptiden durch einen Wirt
SE9401921D0 (sv) * 1994-06-03 1994-06-03 Sbl Vaccin Ab A method of cultivating bacteria
US6368837B1 (en) * 1999-08-06 2002-04-09 E. I. Du Pont Nemours And Company Bioproduction of para-hydroxycinnamic acid
US6521748B2 (en) 1999-08-06 2003-02-18 E. I. Du Pont De Nemours And Company Polynucleotide encoding a mutant Rhodotorula glutinis tyrosine ammonia lyase polypeptide
EP1400593A4 (en) * 2001-06-06 2005-04-06 Takeda Pharmaceutical NEW PROMOTER
EP4015787B1 (en) 2020-12-17 2024-01-24 C.R.F. Società Consortile per Azioni Internal combustion engine with fast combustion, and method for controlling the engine

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60221091A (ja) * 1983-12-21 1985-11-05 Kyowa Hakko Kogyo Co Ltd 新規プロモ−タ−
GB8621626D0 (en) * 1986-09-08 1986-10-15 Health Lab Service Board Phenylalanine ammonia lyase
JP2507345B2 (ja) * 1986-09-16 1996-06-12 三井東圧化学株式会社 Ｌ―フェニルアラニン―アンモニアリ・ア―ゼ構造遺伝子

Also Published As

Publication number Publication date
DK88488D0 (da) 1988-02-19
DE3877012T2 (de) 1993-04-15
EP0279665A3 (en) 1989-06-14
MX168908B (es) 1993-06-14
DK88488A (da) 1988-08-20
KR910001812B1 (ko) 1991-03-26
US5043277A (en) 1991-08-27
EP0279665A2 (en) 1988-08-24
EP0279665B1 (en) 1992-12-30
DE3877012D1 (de) 1993-02-11
US5322786A (en) 1994-06-21
CA1320922C (en) 1993-08-03
KR880010129A (ko) 1988-10-07

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