LU101834B1 - A chloroplast homologous recombinant empty vector of Dunaliella salina and its application - Google Patents

A chloroplast homologous recombinant empty vector of Dunaliella salina and its application Download PDF

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Publication number
LU101834B1
LU101834B1 LU101834A LU101834A LU101834B1 LU 101834 B1 LU101834 B1 LU 101834B1 LU 101834 A LU101834 A LU 101834A LU 101834 A LU101834 A LU 101834A LU 101834 B1 LU101834 B1 LU 101834B1
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LU
Luxembourg
Prior art keywords
seq
sequence shown
salina
chloroplast
empty vector
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LU101834A
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English (en)
French (fr)
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LU101834A1 (en
Inventor
Yulin Cui
Bin Lin
Song Qin
Jinling Chu
Xudong Jiao
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Yantai Institute Of Costal Zone Res Chinese Academy Of Sciences
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C12N1/125—Unicellular algae isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
LU101834A 2019-02-20 2019-06-19 A chloroplast homologous recombinant empty vector of Dunaliella salina and its application LU101834B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910125884.6A CN109536525B (zh) 2019-02-20 2019-02-20 一种杜氏盐藻叶绿体同源重组空载体及其应用

Publications (2)

Publication Number Publication Date
LU101834A1 LU101834A1 (en) 2020-08-20
LU101834B1 true LU101834B1 (en) 2021-02-11

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LU101834A LU101834B1 (en) 2019-02-20 2019-06-19 A chloroplast homologous recombinant empty vector of Dunaliella salina and its application

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CN (1) CN109536525B (zh)
LU (1) LU101834B1 (zh)
WO (1) WO2020168659A1 (zh)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用
CN110669788B (zh) * 2019-12-04 2020-04-07 中国科学院烟台海岸带研究所 一种紫球藻叶绿体表达系统及其应用
CN110669787B (zh) * 2019-12-04 2020-04-17 中国科学院烟台海岸带研究所 一种普通小球藻叶绿体同源重组空载体及其应用
CN110747225B (zh) * 2019-12-24 2020-04-28 中国科学院烟台海岸带研究所 一种斜生栅藻叶绿体同源重组空载体及其应用
CN110747224B (zh) * 2019-12-24 2020-05-01 中国科学院烟台海岸带研究所 一种富脂微拟球藻叶绿体转基因系统及其应用
CN110747226B (zh) * 2019-12-24 2020-04-21 中国科学院烟台海岸带研究所 一种等鞭金藻叶绿体同源重组转基因系统及其应用
CN111909850B (zh) * 2020-07-09 2022-07-26 华南理工大学 基于杜氏盐藻代谢途径和夏侧金盏花cbfd与hbfd的产虾青素工程菌及其构建与应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2698627A1 (en) * 2007-10-05 2009-04-09 Sapphire Energy, Inc. System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts
CN101805743B (zh) * 2010-03-26 2015-11-25 郑州大学 一种提高光合作用效率转基因盐藻的制备方法
CN102121026A (zh) * 2010-12-11 2011-07-13 郑州大学 一种杜氏盐藻叶绿体转化载体的构建方法
CN102191265A (zh) * 2011-04-06 2011-09-21 南开大学 一种杜氏藻基因敲除载体及其应用
EP3215602B1 (en) * 2014-11-05 2019-12-25 The Research Institute at Nationwide Children's Hospital Methods and materials for producing recombinant viruses in eukaryotic microalgae
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用

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Publication number Publication date
CN109536525B (zh) 2019-08-23
CN109536525A (zh) 2019-03-29
LU101834A1 (en) 2020-08-20
WO2020168659A1 (zh) 2020-08-27

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