KR960007612A - 재조합 사람 혈청 알부민의 정제방법 - Google Patents
재조합 사람 혈청 알부민의 정제방법 Download PDFInfo
- Publication number
- KR960007612A KR960007612A KR1019950027882A KR19950027882A KR960007612A KR 960007612 A KR960007612 A KR 960007612A KR 1019950027882 A KR1019950027882 A KR 1019950027882A KR 19950027882 A KR19950027882 A KR 19950027882A KR 960007612 A KR960007612 A KR 960007612A
- Authority
- KR
- South Korea
- Prior art keywords
- human serum
- serum albumin
- recombinant human
- treatment
- albumin
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
본 발명은 제조합 사람 혈청 알부민(rHSA) alc rHSA-생성숙주 세포를 함유하는 배양 배지를 가열하고, 흡착제 입자가 이들과 접촉을 이루도록 현탁되어 있는 유동충내로 가열 용액을 상부를 향해 공급한 후, rHSA를 함유하는 흡착 분획을 회수함으로써 rHSA을 정제하는 방법, 및 25% 알부민 용액으로 제형시 이하의 A350/A280비가 0.015 미만인 rHSA를 함유하는 조성물을 제공한다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 rHSA 배양 배지에 미치는 가열처리의 안정화 효과를 보여주는 그래프도이다.
제3도는 스트림라인(Steamline)SP를 사용하게 (효모 세포를 함유하는)배양 배지로부터 rHSA 정제의 최적 흐름도를 나타내는 작업 공정도이다.
Claims (9)
- 재조합 사람 혈청 알부민 및 이를 생성하는 숙주 세포를 함유하는 배양 배지를 가열하고, 가열 용액을 알부민을 선택적으로 흡착하기 위한 조건하에서 유동층에 현탁된 흡착제 입자와 접촉시킨 다음, 흡착 분획을 회수함을 특징으로 하는, 재조합 사람 혈청 알부민 정제 방법.
- 제1항에 있어서, 재조합 사람 혈청 알부민 및 이를 생성하는 숙주 세포를 함유하는 배양 배지를 가열하고, 알부민을 선택적으로 흡착하기 위한 조건하에서 가열하고, 알부민을 선택적으로 흡착하기 위한 조건하에서 가열용액과 흡착제 입자가 접촉을 이루도록 흡착제 입자가 현탁되어 있는 유동층내로 상부를 향해 가열 용액을 공급한 다음, 유동 방향을 용출시키고 회수함을 특징으로 하는, 재조합 사람 혈청 알부민 정제 방법.
- 제1항 또는 제2항에 있어서, 가열이 50 내지 100℃에서 1분 내지 10시간 동안 수행되는 재조합 사람 혈청 알부민 정제방법.
- 제3항에 있어서, 가열된 용액이 pH 약 3 내지 5에서 흡착제 입자와 접촉되는 재조합 사람 혈청 알부민 정제 방법.
- 제4항에 있어서, 가열 용액이 0.1 내지 50mS의 전기 전도율의 대기중에서 흡착제 입자와 접촉되는 재조합 사람 혈청 알부민 정제방법.
- 제5항에 있어서, 흡착제 입자가 강한 양이온 교환 그룹을 갖는 재조합 사람 혈청 알부민 정제방법.
- 제1항 또는 제2항에 따른 정제 방법에 의해 회수된 사람 혈청 알부민을 함유하는 흡착 분획을 소수성 크로마토그래피, 음이온 교환제 처리, 킬레이트 수지 처리, 붕산/붕산염 처리 및 한외여과막 처리로 이루어진 그룹 중에서 선택된 하나 이상의 정제처리에 추가로 적용시키는 재조합 사람 혈청 알부민 정제방법.
- 제1항 또는 제2항에 따른 정제 방법에 의해 회수된 사람 혈청 알부민을 함유하는 흡착 분획을 환원제의 존재하에 가열시킨 다음, 소수성 크로마토그래피, 음이온 교환제 처리, 킬레이트 수지 처리, 붕산/붕산염 처리 및 한외여과막 처리로 이루어진 그룹 중에서 선택된 하나 이상의 정제처리를 하는 재조합 사람 혈청 알부민 정제방법.
- 25% 알부민 용액으로 제형시 A350/A280 비가 0.015 미만인 재조합 사람 혈청 알부민을 함유하는 사람 혈청 알부민-함유 조성물.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP20770494 | 1994-08-31 | ||
JP94-207704 | 1994-08-31 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR960007612A true KR960007612A (ko) | 1996-03-22 |
KR100392021B1 KR100392021B1 (ko) | 2003-11-01 |
Family
ID=16544190
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019950027882A KR100392021B1 (ko) | 1994-08-31 | 1995-08-31 | 재조합 사람혈청알부민의 정제방법 |
Country Status (9)
Country | Link |
---|---|
US (2) | US5962649A (ko) |
EP (1) | EP0699687B1 (ko) |
KR (1) | KR100392021B1 (ko) |
CN (2) | CN1080301C (ko) |
CA (1) | CA2157219C (ko) |
DE (1) | DE69532492T2 (ko) |
DK (1) | DK0699687T3 (ko) |
ES (1) | ES2211889T3 (ko) |
TW (1) | TW363065B (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20030007186A (ko) * | 2001-07-17 | 2003-01-23 | 미쯔이카가쿠 가부시기가이샤 | 광 양이온성 경화가능 수지 조성물 및 그 용도 |
Families Citing this family (67)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5728553A (en) | 1992-09-23 | 1998-03-17 | Delta Biotechnology Limited | High purity albumin and method of producing |
CA2157219C (en) * | 1994-08-31 | 2010-10-05 | Munehiro Noda | Process for purifying recombinant human serum albumin |
WO1997031947A1 (en) * | 1996-02-29 | 1997-09-04 | Delta Biotechnology Limited | High purity albumin production process |
US6632648B1 (en) | 1996-05-14 | 2003-10-14 | Elan Drug Delivery Limited | Methods of terminal sterilization of fibrinogen |
WO1998006822A1 (en) * | 1996-08-08 | 1998-02-19 | Yoshitomi Pharmaceutical Industries, Ltd. | Culture medium and use of the same |
IL137649A (en) * | 1998-02-18 | 2004-08-31 | Genentech Inc | Method of adsorption chromatography |
US6899889B1 (en) * | 1998-11-06 | 2005-05-31 | Neomend, Inc. | Biocompatible material composition adaptable to diverse therapeutic indications |
US7279001B2 (en) * | 1998-11-06 | 2007-10-09 | Neomend, Inc. | Systems, methods, and compositions for achieving closure of vascular puncture sites |
US6830756B2 (en) * | 1998-11-06 | 2004-12-14 | Neomend, Inc. | Systems, methods, and compositions for achieving closure of vascular puncture sites |
US7351249B2 (en) * | 1998-11-06 | 2008-04-01 | Neomend, Inc. | Systems, methods, and compositions for achieving closure of suture sites |
GB9902000D0 (en) | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
EP1284287A4 (en) * | 2000-05-10 | 2004-10-13 | Mitsubishi Pharma Corp | METHOD FOR PRODUCING A VIRUS VECTOR |
US6787636B1 (en) * | 2000-07-14 | 2004-09-07 | New Century Pharmaceuticals, Inc. | Modified serum albumin with reduced affinity for nickel and copper |
EP1175931A1 (en) * | 2000-07-25 | 2002-01-30 | Computer Cell Culture Center S.A. | Integration of high cell density bioreactor operation with ultra fast on-line downstream processing |
CA2437342C (en) | 2001-02-01 | 2013-08-06 | F. Hoffmann-La Roche Ag | Method for producing recombinant trypsin |
JP2002265383A (ja) * | 2001-03-14 | 2002-09-18 | Mitsubishi Pharma Corp | インターフェロンα注射用液状製剤 |
EP1412384B1 (en) | 2001-06-28 | 2007-12-26 | Novo Nordisk A/S | Stable formulation of modified glp-1 |
ITBO20010426A1 (it) | 2001-07-06 | 2003-01-06 | Alfa Wassermann Spa | Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico |
AU2003211474A1 (en) * | 2002-02-28 | 2003-09-09 | Nipro Corporation | Stabilized albumin preparaions |
SE526227C2 (sv) | 2002-05-15 | 2005-08-02 | North China Pharmaceutical Group | Metod för rening av rekombinant humant serumalbumin |
WO2004048588A1 (en) * | 2002-11-26 | 2004-06-10 | Novo Nordisk A/S | Process for purifying a fermentation-derived product |
EP1608457A1 (en) * | 2003-03-12 | 2005-12-28 | Fresenius Kabi Deutschland GmbH | Use of recombinant albumin in dialysis after liver failure |
JP2007524592A (ja) | 2003-06-03 | 2007-08-30 | ノボ・ノルデイスク・エー/エス | 安定化された薬学的ペプチド組成物 |
EP2292254A3 (en) | 2003-06-03 | 2011-12-14 | Novo Nordisk A/S | Stabilized pharmaceutical peptide compositions |
CA2545034C (en) | 2003-11-20 | 2013-03-05 | Novo Nordisk A/S | Propylene glycol-containing peptide formulations which are optimal for production and for use in injection devices |
JP2005245269A (ja) * | 2004-03-03 | 2005-09-15 | Nipro Corp | 血清アルブミン多量体を含む遺伝子組換え型蛋白質 |
DK1745078T3 (da) * | 2004-04-23 | 2009-10-26 | Conjuchem Biotechnologies Inc | Fremgangsmåde til oprensning af albuminkonjugater |
US9624260B2 (en) | 2004-06-07 | 2017-04-18 | Therapure Biopharma Inc. | Process for isolation of plasma or serum proteins |
CN100374560C (zh) * | 2004-08-27 | 2008-03-12 | 富岩 | 高效表达人血清白蛋白酵母菌株的构建及发酵纯化工艺 |
PL1789434T3 (pl) | 2004-08-31 | 2014-07-31 | Novo Nordisk As | Zastosowanie tris(hydroksymetylo)aminometanu do stabilizacji peptydów, polipeptydów i białek |
EP1817048B1 (en) | 2004-11-12 | 2014-02-12 | Novo Nordisk A/S | Stable formulations of insulinoptropic peptides |
CN1854306B (zh) * | 2005-04-29 | 2011-07-27 | 华北制药集团新药研究开发有限责任公司 | 发酵生产重组人血清白蛋白hsa的方法 |
IL169230A (en) | 2005-06-16 | 2012-03-29 | Plasan Sasa Agricultural Cooperative Soc Ltd | Ballistic armor |
WO2007063129A2 (en) * | 2005-12-02 | 2007-06-07 | Novozymes A/S | Insolation of peptides , polypeptides and proteins |
JP4935242B2 (ja) * | 2006-08-24 | 2012-05-23 | ニプロ株式会社 | 脂肪酸を含有するs−ニトロソタンパク質とその製法 |
US8163886B2 (en) | 2006-12-21 | 2012-04-24 | Emd Millipore Corporation | Purification of proteins |
US8569464B2 (en) | 2006-12-21 | 2013-10-29 | Emd Millipore Corporation | Purification of proteins |
JP2010515441A (ja) * | 2007-01-15 | 2010-05-13 | アップフロント・クロマトグラフィ・アクティーゼルスカブ | 原材料からのバイオ燃料およびタンパク質の製造 |
US20100190963A1 (en) | 2008-12-16 | 2010-07-29 | Millipore Corporation | Stirred Tank Reactor And Method |
KR20120018827A (ko) | 2009-02-20 | 2012-03-05 | 벤트리아 바이오사이언스 | 단백질의 조합을 함유하는 세포 배양 배지 |
CN102190722B (zh) * | 2010-03-16 | 2014-03-26 | 上海欣瑞特生物医药技术有限公司 | 一种纯化重组人血白蛋白的方法 |
ES2754210T3 (es) | 2010-05-17 | 2020-04-16 | Emd Millipore Corp | Polímeros sensibles a estímulos para la purificación de biomoléculas |
CN102532254B (zh) * | 2010-12-24 | 2015-06-24 | 武汉禾元生物科技股份有限公司 | 一种从水稻种子中分离纯化重组人血清白蛋白的方法 |
WO2012177792A2 (en) | 2011-06-24 | 2012-12-27 | Sequenom, Inc. | Methods and processes for non-invasive assessment of a genetic variation |
US9367663B2 (en) | 2011-10-06 | 2016-06-14 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
US9984198B2 (en) | 2011-10-06 | 2018-05-29 | Sequenom, Inc. | Reducing sequence read count error in assessment of complex genetic variations |
US10196681B2 (en) | 2011-10-06 | 2019-02-05 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
US10424394B2 (en) | 2011-10-06 | 2019-09-24 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
CA2850785C (en) | 2011-10-06 | 2022-12-13 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
WO2013109981A1 (en) | 2012-01-20 | 2013-07-25 | Sequenom, Inc. | Diagnostic processes that factor experimental conditions |
US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
US10504613B2 (en) | 2012-12-20 | 2019-12-10 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
US10482994B2 (en) | 2012-10-04 | 2019-11-19 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
US20130309666A1 (en) | 2013-01-25 | 2013-11-21 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
WO2014165596A1 (en) | 2013-04-03 | 2014-10-09 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
WO2014190286A2 (en) | 2013-05-24 | 2014-11-27 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
DK3011051T3 (en) | 2013-06-21 | 2019-04-23 | Sequenom Inc | Method for non-invasive evaluation of genetic variations |
ES2968644T3 (es) | 2013-10-04 | 2024-05-13 | Sequenom Inc | Métodos y procedimientos para la evaluación no invasiva de variaciones genéticas |
JP6680680B2 (ja) | 2013-10-07 | 2020-04-15 | セクエノム, インコーポレイテッド | 染色体変化の非侵襲性評価のための方法およびプロセス |
EP3175000B1 (en) | 2014-07-30 | 2020-07-29 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
ES2780367T3 (es) | 2015-01-01 | 2020-08-25 | Shilpa Medicare Ltd | Nuevo método de purificación eficiente de albumina sérica humana |
EP3491560A1 (en) | 2016-07-27 | 2019-06-05 | Sequenom, Inc. | Genetic copy number alteration classifications |
WO2018140521A1 (en) | 2017-01-24 | 2018-08-02 | Sequenom, Inc. | Methods and processes for assessment of genetic variations |
PL3474820T3 (pl) | 2017-08-24 | 2024-05-13 | Novo Nordisk A/S | Kompozycje glp-1 i ich zastosowania |
EP4106724A1 (en) | 2020-02-18 | 2022-12-28 | Novo Nordisk A/S | Glp-1 compositions and uses thereof |
US11739166B2 (en) | 2020-07-02 | 2023-08-29 | Davol Inc. | Reactive polysaccharide-based hemostatic agent |
US20230331772A1 (en) | 2020-12-08 | 2023-10-19 | Tonghua Anrate Biopharmaceutical Co., Ltd | Method for purification of recombinant proteins |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR570916A (ko) | 1923-09-20 | 1924-05-09 | ||
US4228154A (en) * | 1979-02-26 | 1980-10-14 | Armour Pharmaceutical Company | Purification of plasma albumin by ion exchange chromatography |
KR830002718A (ko) | 1980-04-22 | 1983-05-30 | 가와무라 요시부미 | 피라졸 유도체의 제조법 |
US4665192A (en) * | 1984-03-19 | 1987-05-12 | The Rockefeller University | 2-(2-furoyl)-4(5)-2(furanyl)-1H-imidazole |
FR2649991B2 (fr) * | 1988-08-05 | 1994-03-04 | Rhone Poulenc Sante | Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces |
JPH0671434B2 (ja) * | 1989-09-18 | 1994-09-14 | 株式会社ミドリ十字 | ヒト血清アルブミンの製造方法 |
ES2141715T3 (es) * | 1991-07-12 | 2000-04-01 | Dsm Nv | Procedimiento para la purificacion de albumina de suero. |
JPH07102147B2 (ja) * | 1992-03-16 | 1995-11-08 | 株式会社ミドリ十字 | ヒト血清アルブミンの着色抑制方法 |
US5521287A (en) * | 1992-05-20 | 1996-05-28 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
US5440018A (en) * | 1992-05-20 | 1995-08-08 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
CA2157219C (en) * | 1994-08-31 | 2010-10-05 | Munehiro Noda | Process for purifying recombinant human serum albumin |
-
1995
- 1995-08-30 CA CA2157219A patent/CA2157219C/en not_active Expired - Lifetime
- 1995-08-30 ES ES95113620T patent/ES2211889T3/es not_active Expired - Lifetime
- 1995-08-30 EP EP95113620A patent/EP0699687B1/en not_active Expired - Lifetime
- 1995-08-30 DE DE69532492T patent/DE69532492T2/de not_active Expired - Lifetime
- 1995-08-30 DK DK95113620T patent/DK0699687T3/da active
- 1995-08-31 CN CN95117159A patent/CN1080301C/zh not_active Expired - Lifetime
- 1995-08-31 KR KR1019950027882A patent/KR100392021B1/ko active IP Right Grant
- 1995-08-31 US US08/522,089 patent/US5962649A/en not_active Expired - Lifetime
- 1995-09-11 TW TW084109447A patent/TW363065B/zh not_active IP Right Cessation
-
1999
- 1999-05-24 US US09/317,214 patent/US6617133B1/en not_active Expired - Lifetime
-
2001
- 2001-07-19 CN CN01123056A patent/CN1364643A/zh active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20030007186A (ko) * | 2001-07-17 | 2003-01-23 | 미쯔이카가쿠 가부시기가이샤 | 광 양이온성 경화가능 수지 조성물 및 그 용도 |
Also Published As
Publication number | Publication date |
---|---|
DE69532492D1 (de) | 2004-03-04 |
KR100392021B1 (ko) | 2003-11-01 |
US6617133B1 (en) | 2003-09-09 |
CA2157219A1 (en) | 1996-03-01 |
TW363065B (en) | 1999-07-01 |
CA2157219C (en) | 2010-10-05 |
EP0699687A2 (en) | 1996-03-06 |
ES2211889T3 (es) | 2004-07-16 |
EP0699687A3 (en) | 1996-12-18 |
DK0699687T3 (da) | 2004-04-26 |
DE69532492T2 (de) | 2004-12-02 |
CN1364643A (zh) | 2002-08-21 |
EP0699687B1 (en) | 2004-01-28 |
CN1127299A (zh) | 1996-07-24 |
CN1080301C (zh) | 2002-03-06 |
US5962649A (en) | 1999-10-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR960007612A (ko) | 재조합 사람 혈청 알부민의 정제방법 | |
US4673734A (en) | Porous mineral support coated with an aminated polysaccharide polymer | |
US4138287A (en) | Purifying and isolating method for hepatitis virus to use in preparing vaccine | |
CN100463921C (zh) | 白蛋白纯化 | |
AU4251989A (en) | Method for the purification of proteins | |
JP3940037B2 (ja) | 陽イオン交換クロマトグラフィーを介する薬理学的に活性なタンパク質の精製方法 | |
US5234991A (en) | Porous mineral support coated with an aminated polysaccharide polymer | |
GB2098998A (en) | Process of extraction of lactoferrine and ummunoglobulins of milk | |
KR880000464A (ko) | 단백질의 정제 방법 | |
CN102234332B (zh) | 一种重组人血白蛋白及其融合蛋白的分离纯化工艺 | |
JP3438735B2 (ja) | 上清iv、特にiv−4またはコーンフラクションvまたはそれと類似の上清または画分からヒトアルブミンを単離する方法 | |
KR860008279A (ko) | 트립토판의 정제방법 | |
KR930702381A (ko) | 혈청 알부민의 정제방법 | |
Mizutani et al. | Adsorption of cationic biological materials on controlled pore glass | |
SE8505451D0 (sv) | Purification of l-phenylalanine | |
DE68922790D1 (de) | Verfahren zur Herstellung eines Proteins durch Hefe mit Hilfe eines induzierbaren Systems, Vektoren und entsprechende transformierte Stämme. | |
Mader | Origin of the heterogeneity of peroxidase isoenzyme group Gr from Nicotiana tabacum. I. Conformation | |
MX166995B (es) | Purificacion del factor de necrosis tumoral mediane membranas de politetrafluroetileno microporosas | |
Riethorsi | Affinity chromatography for large-scale purification of coagulation factor VIII. | |
JPS61224996A (ja) | マウスインタ−フエロンの精製法 | |
JPH01165393A (ja) | 組換えヒトエリスロポエチンの精製方法 | |
Lakshmi et al. | The minor anionic form of arylsulphatase B (arylsulphatase Bm) of monkey brain. Purification and phosphoprotein nature | |
JPS61234792A (ja) | マウスインタ−フエロンの精製法 | |
KR920012437A (ko) | 인간 인슐린 유사 성장인자-i의 정제 방법 | |
KR970021092A (ko) | 트롬보모둘린의 정제 방법 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
N231 | Notification of change of applicant | ||
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20130621 Year of fee payment: 11 |
|
FPAY | Annual fee payment |
Payment date: 20140626 Year of fee payment: 12 |
|
FPAY | Annual fee payment |
Payment date: 20150618 Year of fee payment: 13 |