KR930013130A - 복합올리고당의 제조방법 - Google Patents

복합올리고당의 제조방법 Download PDF

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Publication number
KR930013130A
KR930013130A KR1019910024070A KR910024070A KR930013130A KR 930013130 A KR930013130 A KR 930013130A KR 1019910024070 A KR1019910024070 A KR 1019910024070A KR 910024070 A KR910024070 A KR 910024070A KR 930013130 A KR930013130 A KR 930013130A
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South Korea
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producing
cells
lactose
sugar
reaction
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KR1019910024070A
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English (en)
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KR950006951B1 (ko
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인만진
최경호
전봉규
김민홍
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유영학
주식회사 미원
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Priority to KR1019910024070A priority Critical patent/KR950006951B1/ko
Publication of KR930013130A publication Critical patent/KR930013130A/ko
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Publication of KR950006951B1 publication Critical patent/KR950006951B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01243—6G-Fructosyltransferase (2.4.1.243)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

본 발명은 프락토실트랜스피라제 활성 및 β-갈락토시다제의 활성을 갖는 오레오바시디움 플루란스(Aureobasidium pullulans) MW-4218(KFCC-10754)를 알지네이트를 담체로 하여 함께 고정화한 후 설탕과 유당을 기질로 하여 동시에 각각 프락토올리고당과 갈락토올리고당으로 전환시켜 두 올리고당이 혼합된 복합 올리고당을 제조하는 방법에 관한 것으로, 본 발명에 따르면 동일한 미생물에서 유래된 두 효소를 함께 고정화하여 사용하기 때문에 효소의 반응조건 등이 비슷하여 간단하게 반응을 수행할 수 있으며, 두가지 올리고당을 동시에 제조하므로 경제적이다.

Description

복합올리고당의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (6)

  1. 탄소원의 종류에 따라 프라토실트랜스피라제 또는 β-갈락토시다제를 생산할 수 있는 오레오바시디움 플루란스(Aureobasidium pullulans) MW-4218(KFCC-10754)를 각각 설탕 및 유당을 탄소원으로 하는 배지에서 배양하여 얻은 균체를 혼합하여 알지네이트에 고정화시켜 얻은 프락토실 트랜스피라제 활설 및 β-갈락토시다제 활성을 갖는 고정화 세포를 유당과 설탕을 함유하는 기질 용액과 반응시켜 갈락토올리고당과 프락토올리고당을 동시에 제조하는 것을 특징으로 하는 복합 올리고당의 제조방법.
  2. 제1항에 있어서, 고정화 균체는 혼합 균체(습균체)와 알지네이트의 비를 1:5~1:10의 범위로 하여 고정화시켜 얻은 것임을 특징으로 하는 복합 올리고당의 제조방법.
  3. 제1항에 있어서, 기질 용액은 50~90%의 설탕 및 10~60%의 유당을 함유함을 특징으로 하는 복합 올리고당의 제조방법.
  4. 제1항에 있어서, 반응 pH가 5~7인 것을 특징으로 하는 복합 올리고당의 제조방법.
  5. 제1항에 있어서, 반응 온도가 40~60℃인 것을 특징으로 하는 복합 올리고당의 제조방법.
  6. 제1항에 있어서, 반응은 회분식 혹은 연속식으로 수행하는 것을 특징으로 하는 복합 올리고당의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910024070A 1991-12-24 1991-12-24 복합올리고당의 제조방법 KR950006951B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019910024070A KR950006951B1 (ko) 1991-12-24 1991-12-24 복합올리고당의 제조방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910024070A KR950006951B1 (ko) 1991-12-24 1991-12-24 복합올리고당의 제조방법

Publications (2)

Publication Number Publication Date
KR930013130A true KR930013130A (ko) 1993-07-21
KR950006951B1 KR950006951B1 (ko) 1995-06-26

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KR1019910024070A KR950006951B1 (ko) 1991-12-24 1991-12-24 복합올리고당의 제조방법

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102558524B1 (ko) * 2022-10-31 2023-07-21 씨제이제일제당 주식회사 올리고당의 고효율 생산을 위한 신규 아우레오바시디움 플루란스 균주 및 이의 용도

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108384821B (zh) * 2017-12-18 2021-09-14 江苏省农业科学院 一种促进肠道益生菌增殖的低聚糖的制备方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102558524B1 (ko) * 2022-10-31 2023-07-21 씨제이제일제당 주식회사 올리고당의 고효율 생산을 위한 신규 아우레오바시디움 플루란스 균주 및 이의 용도
WO2024096511A1 (ko) * 2022-10-31 2024-05-10 씨제이제일제당 (주) 올리고당의 고효율 생산을 위한 신규 아우레오바시디움 플루란스 균주 및 이의 용도

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