KR960023079A - 고농도 갈락토올리고당의 제조방법 - Google Patents

고농도 갈락토올리고당의 제조방법 Download PDF

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KR960023079A
KR960023079A KR1019940034466A KR19940034466A KR960023079A KR 960023079 A KR960023079 A KR 960023079A KR 1019940034466 A KR1019940034466 A KR 1019940034466A KR 19940034466 A KR19940034466 A KR 19940034466A KR 960023079 A KR960023079 A KR 960023079A
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South Korea
Prior art keywords
high concentration
galactoligosaccharide
producing
enzyme
galactosidase
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KR1019940034466A
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KR0130938B1 (ko
Inventor
김동청
인만진
최경호
양성준
김민홍
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유영학
주식회사 미 원
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Priority to KR1019940034466A priority Critical patent/KR0130938B1/ko
Publication of KR960023079A publication Critical patent/KR960023079A/ko
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Publication of KR0130938B1 publication Critical patent/KR0130938B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01018—Glucose isomerase (5.3.1.18)

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

본 발명은 새로운 조성의 갈락토올리고당 혼합액의 제조방법에 관한 것으로서, β-갈락토시다제와 글루코스이소머라제를 유리 효소의 형태로, 또는 물에 녹지 않는 담체에 함께 고정화한 효소의 형태로, pH 5∼8, 온도 30∼70℃ 조건하에, 농도 30∼65%(w/w)의 유당에 작용시켜 회분식 또는 연속식으로 반응시킴을 특징으로 하는 고농도 갈락토올리고당 혼합액의 제조방법을 제공한다.

Description

고농도 갈락토올리고당의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (5)

  1. 효소를 이용하여 기질인 유당으로부터 갈락트올리고당 혼합액을 제조하는 방법에 있어서, β-갈록토시다제와 글루코스이소머라제를 유리 효소의 형태로, 또는 물에 녹지 않는 담체에 함께 고정화한 효소의 형태로, 유당에 작용시켜 회분식 또는 연속식으로 반응시킴을 특징으로 하는 고농도 갈락토올리고당 혼합액의 제조방법.
  2. 제1항에 있어서, 상기 고정화 효소는 β-갈락토시다제와 글루코스이소머라제를 동일한 담체 또는 서로 다른 담체에 글루코스이소머라제의 활성에 대하여 β-갈락토시다제의 활성이 1∼2배가 되도록 고정화하여 얻은 것임을 특징으로 하는 제조방법.
  3. 제1항에 있어서, 유당의 농도를 30∼65%(w/w)로 함을 특징으로 하는 고농도 갈락토올리고당의 제조방법.
  4. 제1항에 있어서, 기질의 pH를 5∼8로 함을 특징으로 하는 고농도 갈락토올리고당의 제조방법.
  5. 제1항에 있어서, 효소 반응온도를 30∼70℃로 함을 특징으로 하는 고농도 갈락토올리고당의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940034466A 1994-12-15 1994-12-15 고농도 갈락토올리고당의 제조방법 KR0130938B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940034466A KR0130938B1 (ko) 1994-12-15 1994-12-15 고농도 갈락토올리고당의 제조방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940034466A KR0130938B1 (ko) 1994-12-15 1994-12-15 고농도 갈락토올리고당의 제조방법

Publications (2)

Publication Number Publication Date
KR960023079A true KR960023079A (ko) 1996-07-18
KR0130938B1 KR0130938B1 (ko) 1998-04-11

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ID=19401715

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940034466A KR0130938B1 (ko) 1994-12-15 1994-12-15 고농도 갈락토올리고당의 제조방법

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KR (1) KR0130938B1 (ko)

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KR0130938B1 (ko) 1998-04-11

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