KR920009848A - 염기성 섬유아세포 성장인자 및 이의 생산방법 - Google Patents

염기성 섬유아세포 성장인자 및 이의 생산방법 Download PDF

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Publication number
KR920009848A
KR920009848A KR1019910021053A KR910021053A KR920009848A KR 920009848 A KR920009848 A KR 920009848A KR 1019910021053 A KR1019910021053 A KR 1019910021053A KR 910021053 A KR910021053 A KR 910021053A KR 920009848 A KR920009848 A KR 920009848A
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South Korea
Prior art keywords
growth factor
fibroblast growth
content
basic fibroblast
replaced
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KR1019910021053A
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English (en)
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KR100205078B1 (ko
Inventor
피이 세든 앤드류
보렌 피이터
글러즈만 야코브
Original Assignee
알퐁스 아아르 노에
아메리칸 사이아나밋드 캄파니
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Publication of KR920009848A publication Critical patent/KR920009848A/ko
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factors [FGF]
    • C07K14/503—Fibroblast growth factors [FGF] basic FGF [bFGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

내용 없음

Description

염기성 섬유아세포 성장인자 및 이의 생산방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 Glu3, 5, Ser78, 96hbFGF cDNA의 구성이다.
제2도는 천연 hbFGF의 헤파린 HPLC이다. 제3도는 환원된 재조합 hbFGF의 헤파린 HPLC이다.

Claims (28)

  1. 하기 (a)-(c)로 구성되는 군으로부터 선택되는 염기성 섬유아세포 성장인자: (a) 3위치의 알라닌과 5위치의 세린이 글루탐산으로 대체된 약 155 아미노산을 갖는 염기성 섬유아세포 성장인자: (b) 3위치의 알라닌과 5위치의 세린이 글루탐산으로 대체되고, 78과 96위치의 시스테인은 이설파이드 스크램블링을 제거하는 아미노산으로 대체된 약 155 아미노산을 갖는 염기성 섬유아세포 성장인자; 및 (c) N-말단 메티오닌이 없는 (a)와 (b)의 절단 형태.
  2. 제1항에 있어서, 상기 염기성 섬유아세포 성장인자는 재조합체인 염기성 섬유아세포 성장인자.
  3. 제2항에 있어서, 78과 96위치의 시스테인은 세린, 리신, 아스파르트산, 글루탐산, 아스파라긴, 글루타민, 히스티딘, 이소토이신, 로이신, 발린, 페닐알라닌, 티로신, 메티오닌, 쓰레오닌, 프롤린, 알라닌, 글리신, 아르기닌 또는 트립토판으로 대체되는 재조합체인 염기성 섬유아세포 성장인자.
  4. 제3항에 있어서, 78과 96위치의 시스테인은 세린으로 대체되는 재조합체인 염기성 섬유아세포 성장인자.
  5. 제2항에 있어서, glu3, 5hbFGF를 포함하는 재조합체인 염기성 섬유아세포 성장인자.
  6. 제4항에 있어서, glu3, 5ser78, 97hbFGF를 포함하는 재조합체인 염기성 섬유아세포 성장인자.
  7. 제2항에 따른 성장인자를 암호하는 DNA 서열을 포함하는 생물학적 기능이 있는 원형 플라스미드 또는 비루스 DNA 베터.
  8. 제7항에 있어서, pET-3a 유도체를 포함하는 벡터.
  9. 제8항에 있어서, RNA 폴리머라제로부터의 T7프로모터를 함유하는 pT7 Kan와 pET-3a(M13)로 구성되는 군으로부터 선택되는 벡터.
  10. 숙주세포가 상기 인자를 발현하도록 하는 방식으로 제7항의 벡터로 형질전환되거나 트랜스펙트된 원핵생물 또는 진핵생물 숙주세포.
    또는 하나 또는 두개의 카르복실산 또는 황산기로 임의로 치환된 페닐이고; n은 0 내지 4의 정수이고; m은 2 내지 4의 정수이고; x는 1 내지 3의 정수임)로부터 선택된 치환체로 유도체화된 염기성 섬유아세포 성장인자.
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  27. 제26항에 있어서, 상기 염기성 섬유아세포 성장인자를 카르복시메틸화된 섬유아세포 성장인자의 염기성 섬유아세포 성장인자.
  28. 제27항에 있어서, 상기 섬유아세포 성장인자는 glu3, 5hbFGF인 염기성 섬유아세포 성장인자.
    ※참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910021053A 1990-11-23 1991-11-22 염기성 섬유 아세포 성장인자 및 이의 생산방법 KR100205078B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61520290A 1990-11-23 1990-11-23
US7/615,202 1990-11-23
US07/615,202 1990-11-23

Publications (2)

Publication Number Publication Date
KR920009848A true KR920009848A (ko) 1992-06-25
KR100205078B1 KR100205078B1 (ko) 1999-06-15

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US (3) US5310883A (ko)
EP (1) EP0486862B1 (ko)
JP (1) JP3205578B2 (ko)
KR (1) KR100205078B1 (ko)
AT (1) ATE138687T1 (ko)
AU (1) AU646514B2 (ko)
CA (1) CA2055963C (ko)
DE (1) DE69119869T2 (ko)
DK (1) DK0486862T3 (ko)
ES (1) ES2087207T3 (ko)
GR (1) GR3020237T3 (ko)
IE (1) IE74956B1 (ko)
IL (1) IL99984A (ko)
NZ (1) NZ240622A (ko)
PT (1) PT99565B (ko)
ZA (1) ZA919260B (ko)

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Publication number Publication date
IE74956B1 (en) 1997-08-13
KR100205078B1 (ko) 1999-06-15
PT99565B (pt) 1999-04-30
IL99984A0 (en) 1992-08-18
JP3205578B2 (ja) 2001-09-04
EP0486862B1 (en) 1996-05-29
ATE138687T1 (de) 1996-06-15
GR3020237T3 (en) 1996-09-30
ES2087207T3 (es) 1996-07-16
DE69119869D1 (de) 1996-07-04
DE69119869T2 (de) 1996-12-12
JPH04330097A (ja) 1992-11-18
CA2055963C (en) 2002-11-12
US5371206A (en) 1994-12-06
US5302702A (en) 1994-04-12
PT99565A (pt) 1992-10-30
US5310883A (en) 1994-05-10
CA2055963A1 (en) 1992-05-24
ZA919260B (en) 1992-08-26
IE914068A1 (en) 1992-06-03
DK0486862T3 (da) 1996-06-24
EP0486862A1 (en) 1992-05-27
AU646514B2 (en) 1994-02-24
NZ240622A (en) 1994-08-26
IL99984A (en) 1998-12-06
AU8806291A (en) 1992-05-28

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