KR900014599A - 본체 효모 쉬조사카로마이세스 폼베에서의 단백질 합성용 발현 벡터 - Google Patents

본체 효모 쉬조사카로마이세스 폼베에서의 단백질 합성용 발현 벡터 Download PDF

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KR900014599A
KR900014599A KR1019900002596A KR900002596A KR900014599A KR 900014599 A KR900014599 A KR 900014599A KR 1019900002596 A KR1019900002596 A KR 1019900002596A KR 900002596 A KR900002596 A KR 900002596A KR 900014599 A KR900014599 A KR 900014599A
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pombe
expression vector
expression vectors
terminator
expression
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KR1019900002596A
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KR0166582B1 (ko
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브뢰커 미챌
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스타인, 부그
베링베르케 아크티엔게젤샤프트
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음

Description

본체 효모 쉬조사카로마이세스 폼베에서의 단백질 합성용 발현 벡터
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1a도는 플라스미드 pMB322의 작제도이다.
제2도는 쉬조사카로마이세스 폼베(S. pombe)ATG 벡터(pMB340)의 개환도이다.
제3도는 ATG 백터 pMB 340의 보조에 의한 FXIIIa'의 발현 개략도이다.

Claims (9)

  1. 쉬조사카로마이세스 폼베(Schizosgccharomyces pombe)내에서 플라스미드의 안정한 자가복제를 보장하는 벡터 구조물 외에 (a) 쉬조사카로마이세스 속의 프로모터 및 (b) 쉬조사카로마이세스 속의 터미네이터를 함유함을 특징으로 하는, 쉬조사카로마이세스(Schizosgccharomyces)속내에서 외래 유전자의 발현을 위한 발현 벡터.
  2. 제1항에 있어서, (a) 및 (b)가 각각 숙주 종에 대해 상동성인 발현 벡터.
  3. 제2항에 있어서, (a)가 쉬조사카로마이세스 폼베 프로모터이고, (b)가 쉬조사카로마이세스 폼베 터미네이터인, 쉬조사카로마이세스 폼베 내에서 외래 유전자의 발현을 위한 발현 벡터.
  4. 제3항에 있어서, (a)가 쉬조사카로마이세스 폼베 ADH 프로모터이고, (b)가 쉬조사카로마이세스 폼베 액틴 유전자의 터미네이터인 발현 벡터.
  5. 발현 벡터 pMB 332.
  6. "ATG"발현 벡터 pMB 340.
  7. 제 1 ,2 ,3 ,4 ,5 또는 6항에 청구된 벡터를 사용함을 특징으로 하여, 쉬조사카로마이세스 속의 대표적인 종내에서 외래 단백질을 유전공학으로 제조하는 방법.
  8. XIIIa인자의 제조를 위한 제 7항에 따른 방법의 용도.
  9. XIIIa'인자의 제조를 위한 제 7항에 따른 방법의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900002596A 1989-03-02 1990-02-28 분체효모 쉬조사카로마이세스 폼베이에서의 단백질 합성용 발현 벡터 KR0166582B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3906540.5 1989-03-02
DE3906540A DE3906540A1 (de) 1989-03-02 1989-03-02 Expressionsvektoren zur synthese von proteinen in der spalthefe schizosaccharomyces pombe

Publications (2)

Publication Number Publication Date
KR900014599A true KR900014599A (ko) 1990-10-24
KR0166582B1 KR0166582B1 (ko) 1999-01-15

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KR1019900002596A KR0166582B1 (ko) 1989-03-02 1990-02-28 분체효모 쉬조사카로마이세스 폼베이에서의 단백질 합성용 발현 벡터

Country Status (11)

Country Link
US (1) US5663061A (ko)
EP (1) EP0385391B1 (ko)
JP (1) JP3100145B2 (ko)
KR (1) KR0166582B1 (ko)
AT (1) ATE189479T1 (ko)
AU (1) AU623749B2 (ko)
CA (1) CA2011304C (ko)
DE (2) DE3906540A1 (ko)
DK (1) DK0385391T3 (ko)
ES (1) ES2144392T3 (ko)
PT (1) PT93300B (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4113949A1 (de) * 1991-04-29 1992-11-05 Behringwerke Ag Schizosaccharomyces spezifische polypeptide
US6379903B1 (en) * 1999-10-08 2002-04-30 Sigma-Aldrich Co. Purification of recombinant proteins fused to multiple epitopes
MXPA04003333A (es) 2001-10-10 2006-02-22 Neose Technologies Inc Remodelado y glicoconjugacion de peptidos.
EP2298354B1 (en) 2001-10-10 2014-03-19 ratiopharm GmbH Remodelling and glycoconjugation of interferon-beta
US20070026485A1 (en) 2003-04-09 2007-02-01 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods
EP1913143A1 (en) * 2005-07-29 2008-04-23 Pharmexa A/S Improved protein expression
EP2859100B1 (en) * 2012-06-11 2017-08-30 Council of Scientific and Industrial Research An expression vector containing strong promoter useful for high level expression of heterologous genes in schizosaccharomyces pombe and method for production of desired proteins thereof
JP6530846B1 (ja) * 2018-10-09 2019-06-12 アサヒグループホールディングス株式会社 免疫賦活剤及び感染予防方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1164389B (it) * 1983-08-05 1987-04-08 Zambon Spa Derivati n-sostituiti dell'1-(4'-alchiltiofenil)-2-ammino-1,3-propandiolo
AU3145184A (en) * 1983-08-16 1985-02-21 Zymogenetics Inc. High expression of foreign genes in schizosaccharomyces pombe
US4931373A (en) * 1984-05-25 1990-06-05 Zymogenetics, Inc. Stable DNA constructs for expression of α-1 antitrypsin
DK175919B1 (da) * 1986-09-19 2005-06-27 Univ Washington Ekspression af biologisk aktiv faktor XIII
JP2795850B2 (ja) * 1987-03-23 1998-09-10 ザイモジェネティクス，インコーポレイティド 酵母発現ベクター
US4929553A (en) * 1987-05-29 1990-05-29 Canadian Patents & Development Ltd. Protease for specific processing of secreted proteins

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Publication number Publication date
KR0166582B1 (ko) 1999-01-15
EP0385391B1 (de) 2000-02-02
PT93300B (pt) 1997-12-31
ATE189479T1 (de) 2000-02-15
ES2144392T3 (es) 2000-06-16
AU623749B2 (en) 1992-05-21
CA2011304A1 (en) 1990-09-02
EP0385391A3 (de) 1991-07-03
EP0385391A2 (de) 1990-09-05
DE59010897D1 (de) 2000-03-09
US5663061A (en) 1997-09-02
PT93300A (pt) 1990-11-07
JPH02283288A (ja) 1990-11-20
AU5059290A (en) 1990-09-06
CA2011304C (en) 2002-10-01
JP3100145B2 (ja) 2000-10-16
DE3906540A1 (de) 1990-09-13
DK0385391T3 (da) 2000-05-29

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