ES2075068T3 - Procedimiento de preparacion de una proteina mediante levaduras que utiliza un sistema inducible, vectores y cepas transformadas correspondientes. - Google Patents

Procedimiento de preparacion de una proteina mediante levaduras que utiliza un sistema inducible, vectores y cepas transformadas correspondientes.

Info

Publication number
ES2075068T3
ES2075068T3 ES89401871T ES89401871T ES2075068T3 ES 2075068 T3 ES2075068 T3 ES 2075068T3 ES 89401871 T ES89401871 T ES 89401871T ES 89401871 T ES89401871 T ES 89401871T ES 2075068 T3 ES2075068 T3 ES 2075068T3
Authority
ES
Spain
Prior art keywords
yeast
ligand
protein
receptor
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89401871T
Other languages
English (en)
Inventor
Pierre Henry Chambon
Daniel Metzger
John White
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Application granted granted Critical
Publication of ES2075068T3 publication Critical patent/ES2075068T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor

Abstract

LA PRESENTE INVENCION SE REFIERE A UN PROCEDIMIENTO PARA LA PREPARACION DE UNA PROTEINA POR LEVADURAS, SEGUN EL CUAL: SE CULTIVAN CELULAS DE LEVADURA QUE CONTIENEN: UNA SECUENCIA DE ADN, QUE CODIFICA PARA DICHA PROTEINA BAJO EL CONTROL DE ELEMENTOS QUE ASEGURAN SU EXPRESION EN LAS LEVADURAS, CONTENIENDO DICHOS ELEMENTOS UNA SECUENCIA DE CONTROL DE TRANSCRIPCION INDUCTIBLE POR UN COMPLEJO FORMADO POR UN RECEPTOR Y UN LIGANDO, UNA SECUENCIA DE ADN FUNCIONAL EN LA LEVADURA, QUE CODIFICA PARA DICHO RECEPTOR, CONTENIENDO EL RECEPTOR DOS PARTES ESENCIALES, UNA DE LAS CUALES RECONOCE EL LIGANDO, DE MODO QUE FORMA UN COMPLEJO CON DICHO LIGANDO Y LA OTRA SE FIJA SOBRE DICHA SECUENCIA CONTROL DE TRANSCRIPCION, LA PARTE DEL RECEPTOR QUE RECONOCE EL LIGANDO ES DE ORIGEN EUCARIOTA Y EL LIGANDO NO ES NECESARIO EN EL CULTIVO DE CELULAS, PERO ES SUSCEPTIBLE DE PENETRAR EN DICHAS CELULAS CUANDO SE AÑADE EN EL MEDIO DE CULTIVO; SE AÑADE DICHO LIGANDO EN EL MEDIO DE CULTIVO EN UN MOMENTO CONVENIENTE PARA LA INDUCCION; SE RECUPERA LA PROTEINA SINTETIZADA.
ES89401871T 1988-07-01 1989-06-29 Procedimiento de preparacion de una proteina mediante levaduras que utiliza un sistema inducible, vectores y cepas transformadas correspondientes. Expired - Lifetime ES2075068T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8808978A FR2634495B1 (fr) 1988-07-01 1988-07-01 Procede de preparation d'une proteine par des levures utilisant un systeme inductible, vecteurs et souches transformees correspondantes

Publications (1)

Publication Number Publication Date
ES2075068T3 true ES2075068T3 (es) 1995-10-01

Family

ID=9368010

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89401871T Expired - Lifetime ES2075068T3 (es) 1988-07-01 1989-06-29 Procedimiento de preparacion de una proteina mediante levaduras que utiliza un sistema inducible, vectores y cepas transformadas correspondientes.

Country Status (10)

Country Link
US (2) US5817503A (es)
EP (1) EP0349435B1 (es)
JP (1) JP2973430B2 (es)
AT (1) ATE123068T1 (es)
CA (1) CA1341192C (es)
DE (1) DE68922790T2 (es)
DK (1) DK176000B1 (es)
ES (1) ES2075068T3 (es)
FR (1) FR2634495B1 (es)
GR (1) GR3016996T3 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
FR2645174B1 (fr) * 1989-03-31 1994-01-07 Transgene Sa Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
US5879926A (en) * 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
FR2649994A1 (fr) * 1989-06-30 1991-01-25 Aderegem Procede d'obtention d'un recepteur humain natif sauvage des oestrogenes et ses applications
EP0752477A3 (en) * 1990-01-16 1997-05-14 Baylor College Medicine Expression vectors for the production of steroid receptors, chimeras of these receptors, screening tests for these receptors and clinical tests using the synthesized receptors and their vectors
US5733732A (en) * 1996-01-03 1998-03-31 University Of Iowa Research Foundation Methods for detecting primary adhalinopathy
CA2393045A1 (en) * 1999-12-07 2001-06-14 Sumitomo Chemical Co., Ltd. Mutant er.alpha. and test systems for transactivation
US6737262B1 (en) * 2000-07-11 2004-05-18 Robert I. Bolla Animal feed containing polypeptides
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
CA2511359A1 (en) * 2002-12-23 2004-07-15 Glycofi, Inc. Steroid receptor modulation of gene expression

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8620926D0 (en) * 1986-08-29 1986-10-08 Delta Biotechnology Ltd Yeast promoter
US5071773A (en) * 1986-10-24 1991-12-10 The Salk Institute For Biological Studies Hormone receptor-related bioassays
MY103358A (en) * 1987-04-15 1993-06-30 Novartis Ag Process for the production of protiens.

Also Published As

Publication number Publication date
DE68922790T2 (de) 1995-11-30
US6444438B1 (en) 2002-09-03
CA1341192C (fr) 2001-02-27
DK176000B1 (da) 2005-11-14
US5817503A (en) 1998-10-06
DK326289A (da) 1990-01-02
DE68922790D1 (de) 1995-06-29
FR2634495A1 (fr) 1990-01-26
GR3016996T3 (en) 1995-11-30
ATE123068T1 (de) 1995-06-15
EP0349435B1 (fr) 1995-05-24
EP0349435A1 (fr) 1990-01-03
DK326289D0 (da) 1989-06-30
JPH02242693A (ja) 1990-09-27
JP2973430B2 (ja) 1999-11-08
FR2634495B1 (fr) 1990-10-26

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