KR900004936A - 합성 결정형 살충 단백질 및 그의 제조 방법 - Google Patents

합성 결정형 살충 단백질 및 그의 제조 방법 Download PDF

Info

Publication number
KR900004936A
KR900004936A KR1019890013068A KR890013068A KR900004936A KR 900004936 A KR900004936 A KR 900004936A KR 1019890013068 A KR1019890013068 A KR 1019890013068A KR 890013068 A KR890013068 A KR 890013068A KR 900004936 A KR900004936 A KR 900004936A
Authority
KR
South Korea
Prior art keywords
synthetic gene
binding sequence
dna binding
plant
protein
Prior art date
Application number
KR1019890013068A
Other languages
English (en)
Other versions
KR0168038B1 (ko
Inventor
제이 애덩 마이클
에이.로체로이 로마스
제이.메를로 도널드
이.머레이 엘리자베스
Original Assignee
루브리졸 지네틱스, 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22914945&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR900004936(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 루브리졸 지네틱스, 인코포레이티드 filed Critical 루브리졸 지네틱스, 인코포레이티드
Publication of KR900004936A publication Critical patent/KR900004936A/ko
Application granted granted Critical
Publication of KR0168038B1 publication Critical patent/KR0168038B1/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음

Description

합성 결정형 살충단백질 및 그의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 합성 Btt 유전자의 뉴클레오티드 결합서열이다. P544Pst-Met5 에서 발견되는 다른 천연 결합서열은 위에 나타나 있다. 함성 결합서열에서는 잔기 2의 트레오닌을 알라닌으로 잔기 596의 종결 아미산을 루이신으로 치환한 다음 C-말단쪽에 13개의 아미노산을 첨가하여 밑줄친 바와같이 아미노산을 변경시킨다.
제2도는 합성 Btt유전자의 구성과정을 나타내는 개략도이다. A부터 M까지의 분절은 DNA분절을 특이효소적으로 수집하여 바람직한 유전자를 제공하는 독특한 절개위치를 갖는 DNA 듀플렉스를 만들기위해 함께 어닐링하고 결찰시킨 올리고뉴클레오티드 조각이다.
제3도는 합성 Btt 유전자의 구성에서 올리고뉴클레오티드 분절의 수집과정을 나타내는 약도이다. 각 분절(A에서 M까지)은 크기가 다른 뉴클레오티드로 되어있고 바람직한 DNA분절을 만들기위해 어닐링하고 결찰한다.

Claims (15)

  1. Bt의 천연 살충단백질과 같은 기능을 가진 살충단백질을 암호하는 DNA결합서열을 포함하는, 식물 내에서 높은 수준으로 발현하도록 고안한 합성 유전자.
  2. 제1항에 있어서, 상기 DNA 결합서열은 Btt의 천연 살충단백질에 대하여 적어도 85%이상의 상동성을 가지는 합성유전자.
  3. 제1항에 있어서, 상기 DNA 결합서열은 뉴크레오티드 1에서 1793에 걸쳐 제1도에 나타난 바와 같은 합성유전자.
  4. 제1항에 있어서, 상기 DNA 결합서열은 뉴크레오티드 1에서 1833에 걸쳐 제1도에 나타난 바와 같은 합성유전자.
  5. 제1항에 있어서, 상기 합성유전자의 전체 암호화 부위내의 바람직한 암호 사용의 전체 빈도는 식물내의 바람직한 암호 사용 빈도의 약 75%미만인 합성 유전자.
  6. 제1항에 있어서, 상기 DNA 결합서열의 A+5 염기함량이 실제로 식물의 구조유전자의 A+T 염기합량과 동일한 합성유전자.
  7. 제1항에 있어서, 식물의 개시 결합서열이 암호화 부위의 5'말단에 있는 합성유전자.
  8. 제1항에 있어서, 상기 DNA결합서열에서 AATAAA AATGAA, AATAAT, AATATT, GATAAA, GATAAA 및 AATAAG 모티프를 가지는 것을 포함하는 식물의 폴리아데닐화 신호는 삭제화 합성유전자.
  9. 제1항에 있어서, 상기 DNA 결합서열에서 폴리머라제 종결 결합서열인 CAN7-9AGTNNAA를 삭제한 합성 유전자.
  10. 제1항에 있어서, 상기 DNA결합서열에서 CUUCGG 헤어핀을 삭제한 합성유전자.
  11. 제1항에 있어서, 상기 DNA결합서열에서 5'=AAG:GTAAGT 및 3'=TTTT(Pu)TTT(Pu)T(Pu)T(Pu)T(Pu)TGCAG:C를 포함하는 식물의 일치 절개 부위를 삭제한 합성유전자.
  12. 제1항에 있어서, CG 및 TA 더블릿의 기피 지수가 선택한 숙주 식물내에서 높은 수준으로 반현한 유전자의 기피 지수와 실제로 같은 합성유전자.
  13. 제1항의 상기 합성유전자를 포함하는 조환형 클로우닝 벡터.
  14. 제1항의 합성유전자를 포함하는 식물세포.
  15. 해충에 대하여 독성인 단백질 제조방법에 있어서, 식물에서 높은 수준으로 발현되도록 고안한 합성유전자를 포함하며 Bt의 천연 살충 단백질과 동일하게 작용하는 살충 단백질을 암호화하는 DNA 결합서열을 포함하는 DNA 분절을 상기 합성 유전자가 상기 식물 숙주에서 발현되도록 숙주인 식물 세포에 삽입하는 과정으로 구성되는 해충에 대하여 독성인 단백질 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임
KR1019890013068A 1988-09-09 1989-09-09 증진된 식물내 발현을 위한 합성 B.t. 유전자의 제조방법 및 그 유전자 KR0168038B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24248288A 1988-09-09 1988-09-09
US7/242,482 1988-09-09
US07-242,482 1988-09-09

Publications (2)

Publication Number Publication Date
KR900004936A true KR900004936A (ko) 1990-04-13
KR0168038B1 KR0168038B1 (ko) 1999-01-15

Family

ID=22914945

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890013068A KR0168038B1 (ko) 1988-09-09 1989-09-09 증진된 식물내 발현을 위한 합성 B.t. 유전자의 제조방법 및 그 유전자

Country Status (14)

Country Link
US (1) US6013523A (ko)
EP (2) EP0682115A1 (ko)
JP (3) JPH02186989A (ko)
KR (1) KR0168038B1 (ko)
CN (2) CN1145698C (ko)
AT (1) ATE132193T1 (ko)
AU (1) AU623429B2 (ko)
CA (2) CA1341172C (ko)
DE (1) DE68925253T2 (ko)
ES (1) ES2083384T3 (ko)
GR (1) GR3019280T3 (ko)
HK (1) HK1030013A1 (ko)
NZ (1) NZ230375A (ko)
ZA (1) ZA896562B (ko)

Families Citing this family (138)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5254799A (en) * 1985-01-18 1993-10-19 Plant Genetic Systems N.V. Transformation vectors allowing expression of Bacillus thuringiensis endotoxins in plants
BR8600161A (pt) * 1985-01-18 1986-09-23 Plant Genetic Systems Nv Gene quimerico,vetores de plasmidio hibrido,intermediario,processo para controlar insetos em agricultura ou horticultura,composicao inseticida,processo para transformar celulas de plantas para expressar uma toxina de polipeptideo produzida por bacillus thuringiensis,planta,semente de planta,cultura de celulas e plasmidio
TR27832A (tr) 1987-04-29 1995-08-31 Monsanto Co Zararli ucucu hasarata mukavim bitkiler.
US5350689A (en) * 1987-05-20 1994-09-27 Ciba-Geigy Corporation Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells
ES2164633T3 (es) * 1989-02-24 2002-03-01 Monsanto Technology Llc Genes vegetales sinteticos y procedimiento para su preparacion.
US20010003849A1 (en) 1989-08-07 2001-06-14 Kenneth A. Barton Expression of genes in plants
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
CA2074355C (en) 1990-01-22 2008-10-28 Ronald C. Lundquist Method of producing fertile transgenic corn plants
JPH05506578A (ja) * 1990-04-18 1993-09-30 プラント・ジエネテイツク・システムズ・エヌ・ベー 変性ｂａｃｉｌｌｕｓ　ｔｈｕｒｉｎｇｉｅｎｓｉｓ殺虫性結晶タンパク質遺伝子及びそれらの植物細胞中での発現
US5633446A (en) * 1990-04-18 1997-05-27 Plant Genetic Systems, N.V. Modified Bacillus thuringiensis insecticidal-crystal protein genes and their expression in plant cells
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5723747A (en) * 1991-02-22 1998-03-03 Calgene, Inc. Wax esters in transformed plants
US5455167A (en) * 1991-05-21 1995-10-03 Calgene Inc. Medium-chain thioesterases in plants
UA48104C2 (uk) * 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
CA2122261A1 (en) * 1991-10-30 1993-05-13 Marc Cornelissen Modified genes and their expression in plant cells
TW261517B (ko) * 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
EP0694062B1 (en) 1993-04-09 2010-08-04 Bayer BioScience N.V. New bacillus thuringiensis strains and their insecticidal proteins
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US5689052A (en) * 1993-12-22 1997-11-18 Monsanto Company Synthetic DNA sequences having enhanced expression in monocotyledonous plants and method for preparation thereof
US7285416B2 (en) 2000-01-24 2007-10-23 Gendaq Limited Regulated gene expression in plants
US7262055B2 (en) 1998-08-25 2007-08-28 Gendaq Limited Regulated gene expression in plants
CN1176577C (zh) * 1995-10-13 2004-11-24 陶氏农业科学有限公司 编码杀虫性晶体蛋白的核苷酸序列及其应用
US6255558B1 (en) 1996-02-14 2001-07-03 Btg International Limited Gene expression
GB9603069D0 (en) * 1996-02-14 1996-04-10 Medical Res Council Improvements in or relating to gene expression
US6372480B1 (en) 1996-04-19 2002-04-16 Mycogen Corporation Pesticidal proteins
US6677148B1 (en) 1996-04-19 2004-01-13 Mycogen Corporation Pesticidal proteins
US6083499A (en) 1996-04-19 2000-07-04 Mycogen Corporation Pesticidal toxins
WO1998010081A2 (en) * 1996-09-03 1998-03-12 Plant Genetic Systems, N.V. Improved barstar-gene
DE19640817A1 (de) * 1996-10-02 1998-05-14 Hermann Prof Dr Bujard Rekombinantes Herstellungsverfahren für ein vollständiges Malaria-Antigen gp190/MSP 1
US6603063B1 (en) 1999-05-07 2003-08-05 Mycogen Corp. Plants and cells transformed with a nucleic acid from Bacillus thuringiensis strain KB59A4-6 encoding a novel SUP toxin
US6242669B1 (en) * 1996-10-30 2001-06-05 Mycogen Corporation Pesticidal toxins and nucleotide sequences which encode these toxins
US7129212B2 (en) 1996-10-30 2006-10-31 Mycogen Corporation Polynucleotides, pesticidal proteins, and novel methods of using them
NZ335086A (en) 1996-10-30 2000-11-24 Mycogen Corp Bacillus thuringiensis pesticidal toxins and nucleotide sequences which encode these toxins
US6713063B1 (en) 1996-11-20 2004-03-30 Monsanto Technology, Llc Broad-spectrum δ-endotoxins
US6017534A (en) 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
CA2272843C (en) 1996-11-20 2009-11-10 Ecogen, Inc. Broad-spectrum delta-endotoxins
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
AUPO811797A0 (en) 1997-07-18 1997-08-14 University Of Sydney, The Tropoelastin derivatives
CA2299615A1 (en) * 1997-07-31 1999-02-11 Sanford Scientific, Inc. Expression of antimicrobial peptide genes in plants, and their use in creating resistance to multiple plant pathogens
WO1999007210A1 (en) 1997-08-12 1999-02-18 North Carolina State University Genetically engineered duckweed
WO1999020766A2 (en) 1997-10-20 1999-04-29 Genzyme Transgenics Corporation NOVEL MODIFIED NUCLEIC ACID SEQUENCES AND METHODS FOR INCREASING mRNA LEVELS AND PROTEIN EXPRESSION IN CELL SYSTEMS
US5929301A (en) * 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
US6720475B1 (en) 1997-11-18 2004-04-13 Pioneer Hi-Bred International, Inc. Modified nucleic acid sequence encoding FLP recombinase
US6121521A (en) * 1998-04-01 2000-09-19 Novartis Ag Chimeric insecticidal protein and DNA coding therefor
AU4068999A (en) * 1998-04-15 1999-11-01 Prodigene, Inc. Optimized nucleotide sequence encoding organophosphorous hydrolase and methods of use for same
AU5785199A (en) 1998-08-24 2000-03-14 Rutgers, The State University Of New Jersey Synthetic fatty acid desaturase gene for expression in plants
WO2000024903A2 (en) * 1998-10-23 2000-05-04 Mycogen Corporation Plant-optimized polynucleotides encoding pesticidal 43f- and 80jj1/130-type proteins
EP1124967B1 (en) 1998-10-23 2006-05-24 Mycogen Corporation PLANT-OPTIMIZED POLYNUCLEOTIDES ENCODING APPROXIMATELY 15 kDa AND APPROXIMATELY 45 kDa PESTICIDAL PROTEINS
AUPP807799A0 (en) * 1999-01-08 1999-02-04 University Of Queensland, The Polynucleotide and method
US7252966B2 (en) 1999-01-29 2007-08-07 Evolutionary Genomics Llc EG307 polynucleotides and uses thereof
US7439018B2 (en) 1999-01-29 2008-10-21 Evolutionary Genomics, Inc. EG1117 Polynucleotides and uses thereof
CA2370313A1 (en) * 1999-04-21 2000-10-26 University Of Florida Research Foundation, Inc. Materials and methods useful for the control of insect larvae
US6562590B1 (en) 1999-04-21 2003-05-13 University Of Florida Research Foundation, Inc. Transformed cells useful for the control of pests
AU4463000A (en) 1999-04-21 2000-11-02 University Of Florida Research Foundation, Inc. Neuropeptides and their use for pest control
US6593299B1 (en) 1999-04-21 2003-07-15 University Of Florida Research Foundation, Inc. Compositions and methods for controlling pests
WO2001009353A1 (en) * 1999-07-30 2001-02-08 Syngenta Participations Ag Peroxidase gene sequences from tobacco
ES2321375T3 (es) 1999-12-28 2009-06-05 Bayer Bioscience N.V. Proteinas insecticidas de bacillus thuringiensis.
US20110097709A1 (en) * 2000-03-13 2011-04-28 Kidd Geoffrey L Method for modifying a nucleic acid
US20030044972A1 (en) 2000-03-17 2003-03-06 Zoran Ristic Maize chloroplast protein synthesis elongation factors and methods of use for same
AU2001288478B2 (en) 2000-08-25 2006-11-02 Basf Plant Science Gmbh Plant polynucleotides encoding prenyl proteases
ES2397549T3 (es) 2001-01-09 2013-03-07 Bayer Cropscience Nv Proteínas insecticidas de Bacillus thuringiensis
AR035799A1 (es) 2001-03-30 2004-07-14 Syngenta Participations Ag Toxinas insecticidas aisladas de bacillus thuringiensis y sus usos.
WO2002095021A2 (en) * 2001-05-23 2002-11-28 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food A repressor-mediated regulation system for control of gene expression in plants
US20050287647A9 (en) * 2001-05-30 2005-12-29 Carl Perez Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes
US7230167B2 (en) * 2001-08-31 2007-06-12 Syngenta Participations Ag Modified Cry3A toxins and nucleic acid sequences coding therefor
US20080182796A1 (en) * 2001-08-31 2008-07-31 Syngenta Participations Ag Modified Cry3A toxins and nucleic acid sequences coding therefor
EP1947201A3 (en) 2002-01-16 2009-05-06 Evolutionary Genomics, LLC Methods to identify evolutionarily significant changes in polynucleotide and polypeptide sequences in domesticated plants and animals
EP2213681A1 (en) 2002-03-22 2010-08-04 Bayer BioScience N.V. Novel Bacillus thuringiensis insecticidal proteins
DE60230071D1 (de) 2002-06-14 2009-01-08 Monier Tadros Methode für die herstellung von protamin
JP2006508681A (ja) 2002-12-06 2006-03-16 デル・モンテ・フレッシュ・プロデュース・カンパニー カロテノイドレベルを改変したトランスジェニックパイナップル植物体及びその作製方法
US7250296B2 (en) * 2003-03-05 2007-07-31 University Of Florida Research Foundation, Inc. Nucleotide sequences of 2S albumin gene and its promoter from grape and uses thereof
AU2004230490C1 (en) 2003-04-15 2012-08-16 Basf Plant Science Gmbh Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress
ES2389767T3 (es) 2003-05-28 2012-10-31 Basf Se Plantas de trigo que tienen mayor tolerancia a herbicidas de imidazolinona
EP1659855B1 (en) 2003-08-29 2011-11-02 Instituto Nacional de Tecnologia Agropecuaria Rice plants having increased tolerance to imidazolinone herbicides
AU2006236392B2 (en) 2005-04-15 2011-07-21 Del Monte Fresh Produce Company Plant promoters, terminators, genes, vectors and related transformed plants
BRPI0611879A2 (pt) 2005-06-17 2012-08-28 Basf Plant Science Gmbh usos de um ácido nucleico, e de um primeiro ácido nucleico isolado que hibridiza sob condições estringentes para um segundo ácido nucleico, métodos para aumentar a toleráncia de uma planta transgênica contendo o ácido nucleico que codifica o polipeptìdeo relacionado com estresse do tipo lectina de uma proteìna quinase, célula de planta transgênica, planta transgênica, semente, polipeptìdeo, e, seqüência de ácido nucleico
BRPI0613111A2 (pt) 2005-07-08 2010-12-21 Univ Mexico Nacional Autonoma proteìnas bacterianas com atividade pesticida
WO2007011736A2 (en) 2005-07-18 2007-01-25 Basf Plant Science Gmbh Yield increase in plants overexpressing the shsrp genes
US7622573B2 (en) 2006-01-17 2009-11-24 Biolex, Inc. Expression control elements from the lemnaceae family
EP2343377A3 (en) 2006-03-24 2011-11-16 BASF Plant Science GmbH Proteins associated with abiotic stress response and homologs
US20090208955A1 (en) * 2006-05-25 2009-08-20 Institute For Advance Study Methods for identifying sequence motifs, and applications thereof
AU2007356171B8 (en) 2006-08-04 2014-01-16 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them, and methods for making and using them
EP2057178B1 (en) 2006-09-21 2013-11-06 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
DK2479267T3 (en) 2006-12-21 2017-03-27 Basf Enzymes Llc Amylases and Glucoamylases, Nucleic Acids Encoding Them, and Methods for Preparing and Using Them
AU2008231785A1 (en) 2007-03-23 2008-10-02 Basf Plant Science Gmbh Transgenic plant with increased stress tolerance and yield
CA2584934A1 (en) 2007-04-17 2008-10-17 University Of Guelph Nitrogen-regulated sugar sensing gene and protein and modulation thereof
AR066754A1 (es) 2007-05-29 2009-09-09 Basf Plant Science Gmbh Plantas transgenicas con tolerancia al estres y rendimiento aumentados
CA2692650A1 (en) 2007-07-13 2009-01-22 Basf Plant Science Gmbh Transgenic plants with increased stress tolerance and yield
BRPI0814379A2 (pt) 2007-08-02 2017-05-09 Basf Plant Science Gmbh planta transgênica transformada com um cassete de expressão, polinucleotídeo isolado, polipeptídeo isolado, e, métodos para produzir uma planta transgênica, e para aumentar o crescimento e/ou a produção de uma planta sob condições normais ou limitadas de água, e/ou aumentar a tolerância de uma planta e um estresse ambiental
MX2010003878A (es) 2007-10-09 2010-10-15 Athenix Corp Metodos computacionales para diseño genico sintetico.
MX2010003891A (es) * 2007-10-10 2010-09-07 Athenix Corp Genes sinteticos que codifican cry1ac.
CA2706799A1 (en) 2007-11-27 2009-06-04 Basf Plant Science Gmbh Transgenic plants with increased stress tolerance and yield
WO2010020552A1 (en) 2008-08-20 2010-02-25 Basf Plant Science Gmbh Transgenic plants comprising as transgene a phosphatidate cytidylyltransferase
CA2736799A1 (en) 2008-08-25 2010-03-11 Burnham Institute For Medical Research Conserved hemagglutinin epitope, antibodies to the epitope, and methods of use
WO2010034652A1 (en) 2008-09-23 2010-04-01 Basf Plant Science Gmbh Transgenic plants with increased yield
MX2011005604A (es) 2008-11-28 2011-09-01 Merial Ltd Vacuna recombinante para influenza aviar y sus usos.
US8293533B2 (en) 2008-12-19 2012-10-23 E.I. Du Pont De Nemours And Company Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange
DE112010000749T5 (de) 2009-01-28 2012-08-30 Basf Plant Science Company Gmbh Gentechnische Bearbeitung von NF-YB-Transkriptionsfaktoren für gesteigerte Trockenheitsresistenz und erhöhten Ertrag in transgenen Pflanzen
DE112010000693T5 (de) 2009-01-28 2012-11-22 Basf Plant Science Company Gmbh Transgene Pflanzen mit verändertem Stickstoffmetabolismus
MX2011009550A (es) 2009-03-23 2011-10-12 Basf Plant Science Co Gmbh Plantas transgenicas con mecanismos redox alterados y mayor rendimiento.
KR101817253B1 (ko) 2009-05-21 2018-01-10 신젠타 파티서페이션즈 아게 피타제, 이를 코딩하는 핵산 및 그의 제조 및 사용 방법
EP2435562A1 (en) 2009-05-26 2012-04-04 Biolex Therapeutics, Inc. Compositions and methods for production of aglycosylated plasminogen
ES2658622T3 (es) 2009-07-16 2018-03-12 Wageningen Universiteit Regulación de la deficiencia de zinc y tolerancia en plantas
CN102596988B (zh) 2009-10-02 2016-07-13 先正达参股股份有限公司 杀虫蛋白
WO2011074959A1 (en) 2009-12-15 2011-06-23 Edwin Henricus Antonius Holman Transgenic ozone-resistant plants
MX351643B (es) 2009-12-28 2017-10-23 Merial Ltd Antigeno ndv recombinante y usos del mismo.
BR112012023852B1 (pt) 2010-03-12 2020-11-10 Biolex Therapeutics vacinas recombinantes do vírus da língua azul e usos das mesmas
US9096871B2 (en) 2010-10-06 2015-08-04 Bp Corporation North America Inc. Variant CBH I polypeptides with reduced product inhibition
CN103842511A (zh) 2011-03-23 2014-06-04 先锋国际良种公司 产生复合转基因性状基因座的方法
UY34014A (es) * 2011-04-15 2012-11-30 Dow Agrosciences Llc Genes sintéticos para expresar proteínas en células de maíz, construcciones, plantas transgénicas, métodos para controlar pestes y composiciones
WO2012174139A2 (en) 2011-06-14 2012-12-20 Synthon Biopharmaceuticals B.V. Compositions and methods for making and b ioc ont aining auxotrophic transgenic plants
WO2013116750A1 (en) 2012-02-02 2013-08-08 Conicet HaHB11 PROVIDES IMPROVED PLANT YIELD AND TOLERANCE TO ABIOTIC STRESS
EP2814965B1 (en) 2012-02-16 2018-03-21 Syngenta Participations AG Engineered pesticidal proteins
WO2013136274A1 (en) 2012-03-13 2013-09-19 University Of Guelph Myb55 promoter and use thereof
AU2013302947B2 (en) 2012-08-17 2017-06-01 Diaa ALABED Use of a maize untranslated region for transgene expression in plants
US20140123339A1 (en) 2012-10-31 2014-05-01 Pioneer Hi Bred International Inc Transformed Plants Having Increased Beta-Carotene Levels, Increased Half-Life and Bioavailability and Methods of Producing Such
US20140203176A1 (en) 2013-01-23 2014-07-24 Dow Agrosciences Llc Systems and methods for real-time sampling and analysis of biomolecules beneath the surface of biological tissue
US20160304617A1 (en) 2013-06-17 2016-10-20 Asana Biosciences, Llc 5t4-targeted immunofusion molecule and methods
EP3191595B1 (en) 2014-09-12 2019-12-25 E. I. du Pont de Nemours and Company Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use
BR112017012362A2 (pt) 2014-12-12 2018-07-31 Syngenta Participations Ag composições e métodos para controle de pragas de plantas.
WO2017083092A1 (en) 2015-11-10 2017-05-18 Dow Agrosciences Llc Methods and systems for predicting the risk of transgene silencing
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CA3076020A1 (en) 2017-10-02 2019-04-11 Syngenta Participations Ag Engineered pesticidal proteins and methods of controlling plant pests
EP3732295A1 (en) 2017-12-27 2020-11-04 Pioneer Hi-Bred International, Inc. Transformation of dicot plants
WO2020005933A1 (en) 2018-06-28 2020-01-02 Pioneer Hi-Bred International, Inc. Methods for selecting transformed plants
JP2022512817A (ja) 2018-10-31 2022-02-07 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド オクロバクテリウム（Ｏｃｈｒｏｂａｃｔｒｕｍ）媒介植物形質転換のための組成物及び方法
US20220194994A1 (en) 2019-02-20 2022-06-23 Syngenta Crop Protection Ag Engineered pesticidal proteins and methods of controlling plant pests
CN113412333A (zh) 2019-03-11 2021-09-17 先锋国际良种公司 用于克隆植物生产的方法
JP2022527766A (ja) 2019-03-27 2022-06-06 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド 植物外稙片の形質転換
CA3127173A1 (en) 2019-03-28 2020-10-01 Pioneer Hi-Bred International, Inc. Modified agrobacterium strains and use thereof for plant transformation
WO2020214986A1 (en) 2019-04-18 2020-10-22 Pioneer Hi-Bred International, Inc. Embryogenesis factors for cellular reprogramming of a plant cell
EP3772541A1 (en) 2019-08-06 2021-02-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. A method for the production of plants with altered photorespiration and improved co2 fixation
CA3197681A1 (en) 2020-10-21 2022-04-28 Pioneer Hi-Bred International, Inc. Parthenogenesis factors and methods of using same
US20230407324A1 (en) 2020-10-21 2023-12-21 Pioneer Hi-Bred International, Inc. Doubled haploid inducer

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4356270A (en) * 1977-11-08 1982-10-26 Genentech, Inc. Recombinant DNA cloning vehicle
US5380831A (en) * 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5254799A (en) * 1985-01-18 1993-10-19 Plant Genetic Systems N.V. Transformation vectors allowing expression of Bacillus thuringiensis endotoxins in plants
US5182200A (en) * 1985-04-22 1993-01-26 Lubrizol Genetics, Inc. T-dna promoters
KR870004140A (ko) * 1985-10-28 1987-05-07 모리 히데오 살충성 단백질 유전자의 숙주 세포내 발현에 의한 바실러스 튜린지엔시스아이자와이 ipl 아종의 살충성 단백질 제조방법
US5276268A (en) * 1986-08-23 1994-01-04 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US4888282A (en) * 1987-06-04 1989-12-19 The United States Of America As Represented By The Secretary Of Agriculture Synthetic gene for acyl carrier protein
AU2257288A (en) * 1987-08-17 1989-03-09 Plant Genetic Systems N.V. Plants transformed with a dna sequence from bacillus thuringiensis

Also Published As

Publication number Publication date
CA1341428C (en) 2003-04-29
EP0359472B1 (en) 1995-12-27
CA1341172C (en) 2001-01-16
CN1044298A (zh) 1990-08-01
EP0682115A1 (en) 1995-11-15
CN1145698C (zh) 2004-04-14
US6013523A (en) 2000-01-11
AU623429B2 (en) 1992-05-14
CN1263946A (zh) 2000-08-23
JPH02186989A (ja) 1990-07-23
AU4118289A (en) 1990-03-15
ZA896562B (en) 1990-05-30
NZ230375A (en) 1991-07-26
EP0359472A3 (en) 1990-07-25
KR0168038B1 (ko) 1999-01-15
GR3019280T3 (en) 1996-06-30
ATE132193T1 (de) 1996-01-15
DE68925253D1 (de) 1996-02-08
DE68925253T2 (de) 1996-06-13
JPH11266882A (ja) 1999-10-05
JP2002176995A (ja) 2002-06-25
ES2083384T3 (es) 1996-04-16
EP0359472A2 (en) 1990-03-21
HK1030013A1 (en) 2001-04-20
CN1056880C (zh) 2000-09-27

Similar Documents

Publication Publication Date Title
KR900004936A (ko) 합성 결정형 살충 단백질 및 그의 제조 방법
Zhang et al. The complete mitochondrial genome of the Chinese giant salamander, Andrias davidianus (Amphibia: Caudata)
Juan et al. Mitochondrial DNA phylogeny and sequential colonization of Canary Islands by darkling beetles of the genus Pimelia (Tenebrionidae)
Gadaleta et al. The complete nucleotide sequence of the Rattus norvegicus mitochondrial genome: cryptic signals revealed by comparative analysis between vertebrates
Lee et al. Complete sequence of a sea lamprey (Petromyzon marinus) mitochondrial genome: early establishment of the vertebrate genome organization.
Cantatore et al. The complete nucleotide sequence, gene organization, and genetic code of the mitochondrial genome of Paracentrotus lividus
Ready et al. Mitochondrial DNA characterization of populations of Lutzomyia whitmani (Diptera: Psychodidae) incriminated in the peri-domestic and silvatic transmission of Leishmania species in Brazil
Hong et al. Complete nucleotide sequence and organization of the mitogenome of the silk moth Caligula boisduvalii (Lepidoptera: Saturniidae) and comparison with other lepidopteran insects
Fujiwara et al. Molecular mechanism of introduction of the hidden break into the 28S rRNA of insects: implication based on structural studies
DK51092D0 (da) Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
EA199900503A1 (ru) Днк, кодирующая активные дельта-эндотоксины чешуекрылых насекомых, и ее использование
NO864275D0 (no) Fremgangsmaate til seteselektiv genomisk modifisering av gjaer av slekten pichia.
BG38166A3 (en) Method for preparing polypeptide including expression of its heterologic from recombinant microbial cloning vector
KR930702521A (ko) 효율적인 유전적 억제요소를 얻는 방법 및 그 용도
TR200401186T4 (tr) Rekombinant bir RNA-virüs oluşumunu yönlendirebilen nükleik asit sekansları içerikli suni kromozom yapıları.
Ishikawa et al. A mitochondrial DNA phylogeny indicates close relationships between populations of Lutzomyia whitmani (Diptera: Psychodidae, Phlebotominae) from the rain-forest regions of Amazonia and northeast Brazil
RU2377248C2 (ru) Устойчивые к грибкам растения и их использование
Wan et al. Complete mitochondrial genome of a carabid beetle, Damaster mirabilissimus mirabilissim (Coleoptera: Carabidae)
HUP0001817A2 (hu) Chlorella víruspromóterek
CN107142271A (zh) 在基因打靶中具有高突变效率的PL‑LbCpf1‑RR基因及其应用
KR830003576A (ko) 인간의 프로인슐린선구물질과 프로인슐린 유전자를 분리, 정제하고 미생물에게 전달해서 복제하는 방법
Kiff et al. Transposon-induced deletions in unc-22 of C. elegans associated with almost normal gene activity
Hwang et al. High-sequence conservation among the United States bluetongue viruses cognate M2 genes which encode the nonstructural NS1 tubule protein
Gonçalves et al. Structure and genetic variation of the mitochondrial control region in the honey bee Apis mellifera
DE69318716D1 (de) Anti-Katze Immundefiziensvirus (FIV) Impfstoffe

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20090925

Year of fee payment: 12

LAPS Lapse due to unpaid annual fee