KR890016174A - L-트립토판의 제조방법 - Google Patents
L-트립토판의 제조방법 Download PDFInfo
- Publication number
- KR890016174A KR890016174A KR1019890005109A KR890005109A KR890016174A KR 890016174 A KR890016174 A KR 890016174A KR 1019890005109 A KR1019890005109 A KR 1019890005109A KR 890005109 A KR890005109 A KR 890005109A KR 890016174 A KR890016174 A KR 890016174A
- Authority
- KR
- South Korea
- Prior art keywords
- synthase
- anthranilate
- tryptophan
- brevibacterium
- dna
- Prior art date
Links
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 title claims description 8
- 229960004799 tryptophan Drugs 0.000 title claims description 4
- 238000004519 manufacturing process Methods 0.000 title claims 2
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 108020004414 DNA Proteins 0.000 claims 8
- 241000186146 Brevibacterium Species 0.000 claims 5
- 241000186216 Corynebacterium Species 0.000 claims 5
- 244000005700 microbiome Species 0.000 claims 5
- 239000012634 fragment Substances 0.000 claims 4
- 108010037870 Anthranilate Synthase Proteins 0.000 claims 3
- 108010063678 Indole-3-Glycerol-Phosphate Synthase Proteins 0.000 claims 3
- 229910019142 PO4 Inorganic materials 0.000 claims 3
- 108010075344 Tryptophan synthase Proteins 0.000 claims 3
- 230000002068 genetic effect Effects 0.000 claims 3
- 239000010452 phosphate Substances 0.000 claims 3
- 238000003786 synthesis reaction Methods 0.000 claims 3
- 108010086950 Phosphoribosylanthranilate isomerase Proteins 0.000 claims 2
- 108020004511 Recombinant DNA Proteins 0.000 claims 2
- 108090000992 Transferases Proteins 0.000 claims 2
- 102000004357 Transferases Human genes 0.000 claims 2
- 108020001077 Anthranilate Phosphoribosyltransferase Proteins 0.000 claims 1
- 125000005427 anthranyl group Chemical group 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- -1 phosphoribosyl Chemical group 0.000 claims 1
- IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 101150109823 ds gene Proteins 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C12P13/227—Tryptophan
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1도는 제한 효소에 대한 pCDtrp157의 절단지도를 나타낸다. DS 유전자는 pCDtrp157의 검게칠한 부분에 포함되어 있는 반면에, L-트립토판 생합성에 관한 유전자는 pCDtrp157의 빗살친 부분에 포함되어 있다. 플라스미드의 크기는 킬로염기(Kb)로 나타낸다.
Claims (4)
- 코리네박테리움(Corynebacterium) 또는 브레비박테리움(Brevibacterium)속에 속하고 벡터 DNA 및 3-데옥시-D-아라비노-헵투로소네이트-7-포스페이트 신타제, 안트라닐레이트 신타제, 안트라닐레이트 포스포리보실 트랜스퍼라제, N-5'-포스포리보실 안트라닐레이트 이소메타제, 인돌-3-글리세롤 포스페이트 신타제 및 트립토판 신타제의 합성에 관한 모든 유전 정보를 가진 DNA 단편으로 이루어진 제조합 DNA 를 보유하는 미생물을 배양 배지에서 배양하고 ; L-트립토판을 배양액에 축적시키고 ; 여기에서 L-트립토판을 회수함을 특징으로 하는 L-트립토판의 제조방법.
- 제 1 항에 있어서, DNA 단편이 코리네박테리움 또는 브레비박테리움 속에 속하는 미생물로부터 유래된 제조방법.
- 벡터 DNA 및 코리네박테리움 또는 브레비박테리움 속에 속하는 미생물로부터 유래되고 3-데옥시-D-아라비노-헵투로소네이트-7-포스페이트 신타제, 안트라닐레이트 신타제, 안트라닐레이트 포스포리보실 트랜스퍼라제, N-5'-포스포리보실 안트라닐레이트 이소메타제, 인돌-3-글리세롤 포스페이트 신타제 및 트립토판 신타제의 합성에 관한 모든 유전 정보를 가진 DNA 단편으로 이루어진 재조합 DNA.
- 코리네박테리움 또는 브레비박테리움 속에 속하고, 벡터 DNA 및 코리네박테리움 또는 브레비박테리움 속에 속하고 3-데옥시-3-D-아라비노-헵투로소네이트-7-포스페이트 신타제, 안트라닐레이트 신타제, 안트라닐레이트 포스포리보실 트랜스퍼라제, N-5'-포스포리보실 안트라닐레이트 이소메라제, 인돌-3-글리세롤 포스페이트 신타제 및 트립토판 신타제의 합성에 관한 모든 유전 정보를 보유하는 미생물로부터 유래된 DNA 단편으로 이루어진 재조합 DNA를 보유하는 미생물.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63093540A JP2656300B2 (ja) | 1988-04-18 | 1988-04-18 | Ｌ−トリプトファンの製造法 |
| JP93540 | 1988-04-18 | ||
| JP93540/88 | 1988-04-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR890016174A true KR890016174A (ko) | 1989-11-28 |
| KR920009500B1 KR920009500B1 (ko) | 1992-10-17 |
Family
ID=14085105
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019890005109A KR920009500B1 (ko) | 1988-04-18 | 1989-04-18 | L-트립토판의 제조방법 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US5563052A (ko) |
| EP (1) | EP0338474B1 (ko) |
| JP (1) | JP2656300B2 (ko) |
| KR (1) | KR920009500B1 (ko) |
| DE (1) | DE68923643T2 (ko) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3078312B2 (ja) * | 1990-11-22 | 2000-08-21 | 協和醗酵工業株式会社 | 物質の製造法 |
| DE4232468A1 (de) | 1992-09-28 | 1994-03-31 | Consortium Elektrochem Ind | Mikroorganismen für die Produktion von Tryptophan und Verfahren zu ihrer Herstellung |
| JP3369231B2 (ja) * | 1992-12-03 | 2003-01-20 | 協和醗酵工業株式会社 | 芳香族アミノ酸の製造法 |
| US6118047A (en) | 1993-08-25 | 2000-09-12 | Dekalb Genetic Corporation | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
| DE4424818A1 (de) * | 1994-07-14 | 1996-01-18 | Basf Ag | Niederviskose Mischungen aus amphiphilen nicht-ionischen Pfropfcopolymeren und viskositätserniedrigenden Zusätzen |
| DE102005032429A1 (de) | 2005-01-19 | 2006-07-20 | Degussa Ag | Allele des mqo-Gens aus coryneformen Bakterien |
| DE102005013676A1 (de) | 2005-03-24 | 2006-09-28 | Degussa Ag | Allele des zwf-Gens aus coryneformen Bakterien |
| DE102005023829A1 (de) | 2005-05-24 | 2006-11-30 | Degussa Ag | Allele des opcA-Gens aus coryneformen Bakterien |
| DE102005047596A1 (de) | 2005-10-05 | 2007-04-12 | Degussa Ag | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien |
| DE102011006716A1 (de) | 2011-04-04 | 2012-10-04 | Evonik Degussa Gmbh | Mikroorganismus und Verfahren zur fermentativen Herstellung einer organisch-chemischen Verbindung |
| DE102011118019A1 (de) | 2011-06-28 | 2013-01-03 | Evonik Degussa Gmbh | Varianten des Promotors des für die Glyzerinaldehyd-3-phosphat-Dehydrogenase kodierenden gap-Gens |
| EP2762571A1 (de) | 2013-01-30 | 2014-08-06 | Evonik Industries AG | Mikroorganismus und Verfahren zur fermentativen Herstellung von Aminosäuren |
| CN105143440B (zh) * | 2013-04-16 | 2019-06-14 | Cj第一制糖株式会社 | 具有l-色氨酸生产力的微生物以及使用所述微生物生产l-色氨酸的方法 |
| US11723945B2 (en) | 2017-04-25 | 2023-08-15 | Temple Otorongo Llc | Pharmaceutical composition comprising tryptophan and phyllokinin derivative for use in treating psychiatric and psychological conditions |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59156292A (ja) * | 1983-02-17 | 1984-09-05 | Kyowa Hakko Kogyo Co Ltd | トリプトフアンの製造法 |
| WO1984003301A1 (en) * | 1983-02-17 | 1984-08-30 | Kyowa Hakko Kogyo Kk | Process for preparing amino acid |
| JPS6251980A (ja) * | 1985-08-30 | 1987-03-06 | Ajinomoto Co Inc | 発酵法によるｌ―トリプトファンの製造法 |
| DE3576523D1 (de) * | 1984-11-20 | 1990-04-19 | Ajinomoto Kk | Rekombinante dna enthaltende coryneformbakterien und verfahren zur herstellung von aromatischen aminosaeuren unter verwendung dieser bakterien. |
| JPS61149082A (ja) * | 1984-12-25 | 1986-07-07 | Ajinomoto Co Inc | 発酵法によるｌ―トリプトファンの製造法 |
| JPS6265691A (ja) * | 1985-09-17 | 1987-03-24 | Kyowa Hakko Kogyo Co Ltd | 芳香族アミノ酸の製造法 |
| JPS6279775A (ja) * | 1985-10-04 | 1987-04-13 | Ajinomoto Co Inc | 発酵法によるレートリプトファンの製造法 |
| JPH06102024B2 (ja) * | 1986-04-16 | 1994-12-14 | 味の素株式会社 | 新規プロモーター及び該プロモーターを用いた遺伝子発現方法 |
-
1988
- 1988-04-18 JP JP63093540A patent/JP2656300B2/ja not_active Expired - Lifetime
-
1989
- 1989-04-17 DE DE68923643T patent/DE68923643T2/de not_active Expired - Lifetime
- 1989-04-17 EP EP89106813A patent/EP0338474B1/en not_active Expired - Lifetime
- 1989-04-18 KR KR1019890005109A patent/KR920009500B1/ko not_active IP Right Cessation
-
1994
- 1994-05-18 US US08/245,823 patent/US5563052A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0338474B1 (en) | 1995-08-02 |
| KR920009500B1 (ko) | 1992-10-17 |
| EP0338474A2 (en) | 1989-10-25 |
| JPH01265892A (ja) | 1989-10-23 |
| DE68923643D1 (de) | 1995-09-07 |
| EP0338474A3 (en) | 1990-05-30 |
| US5563052A (en) | 1996-10-08 |
| DE68923643T2 (de) | 1996-01-11 |
| JP2656300B2 (ja) | 1997-09-24 |
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