KR850005496A - Dna 및 그의 이용 - Google Patents

Dna 및 그의 이용 Download PDF

Info

Publication number
KR850005496A
KR850005496A KR1019840007786A KR840007786A KR850005496A KR 850005496 A KR850005496 A KR 850005496A KR 1019840007786 A KR1019840007786 A KR 1019840007786A KR 840007786 A KR840007786 A KR 840007786A KR 850005496 A KR850005496 A KR 850005496A
Authority
KR
South Korea
Prior art keywords
bacillus
guanosine
kaist
ifo
ferm
Prior art date
Application number
KR1019840007786A
Other languages
English (en)
Other versions
KR920001378B1 (ko
Inventor
겐이찌로 미야가와 (외 3)
Original Assignee
구라바야시 이꾸시로
다께다 야꾸힝 고오교 가부시끼 가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 구라바야시 이꾸시로, 다께다 야꾸힝 고오교 가부시끼 가이샤 filed Critical 구라바야시 이꾸시로
Publication of KR850005496A publication Critical patent/KR850005496A/ko
Application granted granted Critical
Publication of KR920001378B1 publication Critical patent/KR920001378B1/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

내용 없음

Description

DNA 및 그의 이용
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도, 제2도 및 제3도는 각가 실시예에서 수득된 재조합 플라스미드 pEX117, pEX147 및 pBX121의 제한 효소 절단지도를 나타낸다. 괄호안의 수치는 염기상의 수(킬로염기쌍)이다.

Claims (7)

  1. 5'-이노시네이트 탈수소 효소 유전자 및 그로부터 떨어진 Hind Ⅲ절단 부위 2.9킬로 염기쌍을 갖는 DNA.
  2. 제1항에 있어서, 구아노신 및/또는 크산토신을 생산할 수 있는 바실루스 균주의 염색체 DNA로부터 수득된 DNA.
  3. 제2항에 있어서, 바실루스 균주가 바실루스 서브틸리스 NA-6011 (IFO 14189, FERM BP-291, KAIST 830719-10410), 바실루스 서브틸리스 NA 6012-(IFO 14190, FERM BP-292, KAIST 830719-10510), 바실루스 서크틸리스 NA-7821 (IFO 14368, FERM BP-618, KAIST 841109-14111), 바실루스 서브틸리스 ATCC 19221, 바실루스 푸밀루스 NA-1102 (IFO 14185, FERM BP-289, KAIST 830719-10210) 또는 바실루스 푸밀루스 NA-1103 (IFO 14186, FERM BP-290, KAIST 830719-10310)인 DNA.
  4. 제2항의 DNA가 삽입된 벡타.
  5. 제4항의 벡타로 형질전환된 바실루스 균주.
  6. 제5항에 있어서, 제4항의 벡타로 형질전환 되었으며, 구아노신을 생산할 수 있는 바실루스 균주.
  7. 구아노신-및/또는 크산토신 생산 바실루스 균주의 염색체 DAN로 부터 수득된 5'-이노시네이트 탈수소 효소유전자 부분이 삽입된 벡타에 의해 형질전환된 구아노신 생산 바실루스 균주를 배지에서 배양하고, 배양액에 생성 및 축적된 구아노신을 수거함을 특징으로하는 구아노신의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019840007786A 1984-01-27 1984-12-08 Dna 및 그의 이용 KR920001378B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP13968/1984 1984-01-27
JP59013968A JPH0630583B2 (ja) 1984-01-27 1984-01-27 Ｄνａおよびその用途
JP13968 1984-01-27

Publications (2)

Publication Number Publication Date
KR850005496A true KR850005496A (ko) 1985-08-26
KR920001378B1 KR920001378B1 (ko) 1992-02-11

Family

ID=11848013

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019840007786A KR920001378B1 (ko) 1984-01-27 1984-12-08 Dna 및 그의 이용

Country Status (7)

Country Link
US (1) US4749650A (ko)
EP (1) EP0151341B1 (ko)
JP (1) JPH0630583B2 (ko)
KR (1) KR920001378B1 (ko)
AT (1) ATE40405T1 (ko)
DE (1) DE3476422D1 (ko)
ES (1) ES8602134A1 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2058133T3 (es) * 1986-12-26 1994-11-01 Takeda Chemical Industries Ltd Adn que codifica una imp-deshidrogenasa inactivada.
JPS6427477A (en) * 1987-04-01 1989-01-30 Takeda Chemical Industries Ltd Dna and use thereof
JP4760711B2 (ja) 2004-03-31 2011-08-31 味の素株式会社 バチルス属又はエシェリヒア属に属する細菌を使用した発酵によるプリンヌクレオシド及びヌクレオチドの製造方法
US7326546B2 (en) 2005-03-10 2008-02-05 Ajinomoto Co., Inc. Purine-derived substance-producing bacterium and a method for producing purine-derived substance
BRPI0709635A2 (pt) 2006-04-24 2011-07-19 Ajinomoto Kk bactéria pertencendo ao gênero bacillus, e, métodos para produzir uma substáncia derivada de purina, e um nucleotìdeo de purina
JP5104754B2 (ja) 2006-04-24 2012-12-19 味の素株式会社 プリン系物質生産菌及びプリン系物質の製造法
RU2365622C2 (ru) 2006-12-22 2009-08-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПРОДУКЦИИ ПУРИНОВЫХ НУКЛЕОЗИДОВ И НУКЛЕОТИДОВ МЕТОДОМ ФЕРМЕНТАЦИИ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ, ПРИНАДЛЕЖАЩИХ К РОДУ Escherichia ИЛИ Bacillus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT74811B (en) * 1981-04-29 1983-10-26 Biogen Nv Bacillus cloning vectors recombinant dna molecules bacillus hosts transformed with them and methods for expressing foreign dna sequences and producing polypeptides coded thereby
JPS58175493A (ja) * 1982-04-08 1983-10-14 Ajinomoto Co Inc 発酵法によるグアノシンの製造法

Also Published As

Publication number Publication date
KR920001378B1 (ko) 1992-02-11
ATE40405T1 (de) 1989-02-15
DE3476422D1 (en) 1989-03-02
EP0151341A1 (en) 1985-08-14
EP0151341B1 (en) 1989-01-25
JPH0630583B2 (ja) 1994-04-27
ES537637A0 (es) 1985-11-16
JPS60156388A (ja) 1985-08-16
ES8602134A1 (es) 1985-11-16
US4749650A (en) 1988-06-07

Similar Documents

Publication Publication Date Title
ATE68204T1 (de) Transformation von hefe des genus pichia.
KR850007983A (ko) 히알우론산 생산의 발효법
MX165920B (es) Un procedimiento para producir glucoamilasa
KR850005496A (ko) Dna 및 그의 이용
KR860007377A (ko) 충치예방제의 제조방법
KR910001062A (ko) L-트립토판의 제조방법
KR940019865A (ko) 발효법에 의한 l-글루탐산의 제조법
KR880004091A (ko) L-아르기닌의 제조 방법
KR850001279A (ko) α-아밀라제의 제법
DE3582737D1 (de) Verfahren zur herstellung von 6-hydroxynikotinsaeure.
ATE44766T1 (de) Verfahren zur vergroesserung der expression eines bestimmten gens im bacillus subtilis und erhaltene staemme.
KR910004807A (ko) 변형된 dna 및 그 용도
KR850004275A (ko) 프로모터 및 그의 이용
KR930018034A (ko) 트레할로오스의 제조방법
KR890013180A (ko) 피리미딘 유사체 내성 유전자 dna 및 그의 용도
KR890006827A (ko) 발효법에 의한 오로트산의 제조방법
KR880014106A (ko) 안정한 크레아틴 아미디노 가수분해효소 돌연변이체
KR880001824A (ko) 미생물에 의한 5'-이노신산의 제조방법
ATE2339T1 (de) Verfahren zur herstellung von sphingomyelinase, sphingomyelinase und deren verwendung.
BR8502586A (pt) Processo para preparar uma cepa de b.subtilis
US4916067A (en) Method for the preparation of sorbic acid by oxidizing 2,4-hexadienal with a microorganism
KR910008127A (ko) 글루타민(Glutamine)을 생산하는 미생물
KR890006825A (ko) D-알라닌의 제조방법
KR880001822A (ko) 미생물에 의한 5'-구아닐산의 제조방법
ES2007758A6 (es) Un procedimiento para la obtencion de alfa-amilasas termoestables, por cultivo de microorganismos superproductores a altas temperaturas.

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
G160 Decision to publish patent application
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20020206

Year of fee payment: 11

LAPS Lapse due to unpaid annual fee