KR880001811A - 전분 액화용 알파-아밀라아제 혼합물 - Google Patents

전분 액화용 알파-아밀라아제 혼합물 Download PDF

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KR880001811A
KR880001811A KR1019870007324A KR870007324A KR880001811A KR 880001811 A KR880001811 A KR 880001811A KR 1019870007324 A KR1019870007324 A KR 1019870007324A KR 870007324 A KR870007324 A KR 870007324A KR 880001811 A KR880001811 A KR 880001811A
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amylase
mixture
bacillus
activity
measured
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KR1019870007324A
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KR960006119B1 (ko
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오 케롤 존
알 스완슨 디모디
씨 트랙맨 필립
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노보 인두스트리 아크티에 셀스카브
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase

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  • Chemical & Material Sciences (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Abstract

내용 없음

Description

전분 액화용 알파-아밀라아제 혼합물
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (7)

  1. 바실러스 리세니포르미스와 바실러스 스테아로서모필러스로부터 유도된 α-아밀라아제의 혼합물로 이루어지며, 상기 α-아밀라아제 혼합물은 NU로 측정된 100%의 혼합물의 총 α-아밀라아제 활성도를 기준으로 바실러스 리세니포르미스 α-아밀라아제의 NU에 있어서 측정된 활성도 10 내지 90%를 함유하는 것을 특징으로 하는 α-아밀라아제 조성물.
  2. 제1항에 있어서, α-아밀라아제 혼합물은 바실러스 리세니포르미스 α-아밀라아제의 NU로 측정된 확성도 25 내지 50%를 함유하는 것을 특징으로 하는 α-아밀라아제 조성물.
  3. 바실러스 리세니포르미스와 바실러스 스테아로서모필러스로부터 유도된 α-아밀라아제의 혼합물로 이루어지며, 상기 α-아밀라아제은 NU로 측정된 1005의 혼합물의 총 α-아밀라아제 활성도를 기준으로 바실러스 리세니포르미스 α-아밀라아제의 NU로 측정된 활성도 10내지 90%를 함유하는 α-아밀라아제 조성물로 액화과정을 수행하는 것을 특징으로 하는 전분 또는 전분낟알등의 슬러리를 액화시키는 방법.
  4. 제3항에 있어서, α-아밀라아제 혼합물을 NU로 측정된 100%의 혼합물의 총 α-아밀라아제 활성도를 기준으로 바실러스 리세니포르미스 α-아밀라아제의 NU로 측정된 활성도 25내지 50%를 함유하는 것을 특징으로 하는 방법.
  5. 제3항 또는 제4항에 있어서, α-아밀라아제 조성물의 투여량 수준은 전분 슬러리의 건조고체(DS) g당 100NU를 초과하지 않는 것을 특징으로 하는 방법.
  6. 제5항에 있어서, α-아밀라아제 조성물의 투여량은 gDS당 40내지 80NU의 범위인 것을 특징으로 하는 방법.
  7. 제3항 내지 제6항 중 어느 하나에 있어서, 액화는 100 내지 115℃ 범위의 온도에서 1내지 60분간 제트-쿠킹시키고 이어서, 온도를 90내지 100℃ 로 감소시키고 반응혼합물을 30내지 120분간 유지시킴으로써 행하여 그후 이렇게 액화된 전분은 노화에 대해 안정하며, pH는 과정을 통해 5.5내지 6.0으로 유지되는 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870007324A 1986-07-09 1987-07-08 전분 액화용 알파-아밀라아제 혼합물 KR960006119B1 (ko)

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US88356686A 1986-07-09 1986-07-09
US883566 1986-07-09
US883,566 1992-05-15

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KR880001811A true KR880001811A (ko) 1988-04-27
KR960006119B1 KR960006119B1 (ko) 1996-05-09

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EP (1) EP0252730B1 (ko)
JP (1) JP2509233B2 (ko)
KR (1) KR960006119B1 (ko)
AT (1) ATE80657T1 (ko)
CA (1) CA1283376C (ko)
DE (1) DE3781732T2 (ko)
DK (1) DK166460B1 (ko)
ES (1) ES2052565T3 (ko)
FI (1) FI89076C (ko)

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ATE80657T1 (de) 1992-10-15
DK351387D0 (da) 1987-07-08
FI89076C (fi) 1993-08-10
DE3781732T2 (de) 1993-03-25
KR960006119B1 (ko) 1996-05-09
CA1283376C (en) 1991-04-23
DK166460B1 (da) 1993-05-24
DE3781732D1 (de) 1992-10-22
FI873027A0 (fi) 1987-07-08
FI89076B (fi) 1993-04-30
EP0252730A3 (en) 1988-11-09
JP2509233B2 (ja) 1996-06-19
EP0252730B1 (en) 1992-09-16
ES2052565T3 (es) 1994-07-16
EP0252730A2 (en) 1988-01-13
JPS63102694A (ja) 1988-05-07
DK351387A (da) 1988-01-10
FI873027A (fi) 1988-01-10

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