KR850007274A - 인체 r-인터페론활성을 감는 폴리펩타이드의 제조방법 - Google Patents
인체 r-인터페론활성을 감는 폴리펩타이드의 제조방법 Download PDFInfo
- Publication number
- KR850007274A KR850007274A KR1019850002625A KR850002625A KR850007274A KR 850007274 A KR850007274 A KR 850007274A KR 1019850002625 A KR1019850002625 A KR 1019850002625A KR 850002625 A KR850002625 A KR 850002625A KR 850007274 A KR850007274 A KR 850007274A
- Authority
- KR
- South Korea
- Prior art keywords
- cys
- amino acid
- acid sequence
- host microorganism
- nucleotide sequence
- Prior art date
Links
- 230000000694 effects Effects 0.000 title claims 2
- 229940079322 interferon Drugs 0.000 title description 3
- 238000004519 manufacturing process Methods 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 238000004804 winding Methods 0.000 title 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 6
- 244000005700 microbiome Species 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 108010074328 Interferon-gamma Proteins 0.000 claims 3
- 108020004705 Codon Proteins 0.000 claims 2
- 102000008070 Interferon-gamma Human genes 0.000 claims 2
- 229940044627 gamma-interferon Drugs 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 1
- 108700005078 Synthetic Genes Proteins 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 239000013612 plasmid Substances 0.000 description 10
- 239000012634 fragment Substances 0.000 description 6
- 108010050904 Interferons Proteins 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000014150 Interferons Human genes 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/57—IFN-gamma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/811—Interferon
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/14—Lymphokine; related peptides
- Y10S930/142—Interferon
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 유전자단편 IFN-1을 pBR 322로 삽입시킨 하이브리드 플라스미드를 나타내고, 제2도는 유전자 단편 IFN-2를 pUC 8으로 삽입시킨 하이브리드 플라스미드를 나타내며, 제3도는 유전자단편 IFN-Ⅲ를 pUC 8으로 삽입시킨 하이브리드 플라스미드를 나타내고, 제4도는 DNA-서열 I 을 pUC 8으로 삽입시킨 하이브리드 플라스미드를 나타내며, 제5도는 DNA-서열 IB를 pUC 8으로 삽입시킨 하이브리드 플라스미드를 나타내고, 제6도는 DNA-서열 IA를 pUC 8으로 삽입시킨 하이브리드 플라스미드를 나타내며, 제7도는 DNA-서열 IC를 pMX2로 삽입시킨 하이브리드 플라스미드를 나타내며, 제8도는-인터페론 유전자단편 IA*를 pMX2로 삽입시킨 하이브리드 플라스미드를 나타내고, 제9도는-인터페론 유전자단편 IB를 pMX2M로 삽입시킨 하이브리드 플라스미드를 나타내며, 제10도는-인터페론 유전자단편 IC*를 pMX2로 삽입시킨 하이브리드 플라스미드를 나타낸다.
Claims (3)
- 일반식(1)의 DNA를 갖는 합성유전자를 숙주미생물에서 발현시킴을 특징으로 하여, 아미노산 서열 5내지 127, 1내지 127 및 5내지 146으로 이루어진 부분서열의 인체감마 인터페론(IFN-)또는 이들부분 서열 및 감마 인터페론활성을 갖는 인체감마 인터페론의 동족체(이때, 동족체중의 천연의 아미노산서열은 아미노산의 교환, 삽입 또는 제거에 의해서 변형되었다)를 유전공학적으로 제조하는 방법.(Pr)X-XY-R-YZ-Z (1)상기식에서, x,y 및 z는, 각기 독립적으로, 0 또는 1을 나타내나 X가 Met-Cys-Tyr-Cys-Gln인 경우에는 y 및 z가 모두 다 1은 아니며, Pr은 숙주미생물내에서 또는 시험관내에서 제거되는 프리펩타이드를 코드화하는 뉴클레오티드 서열이고, X는 Met, Met-Cys-Tyr-Cys-Gln 또는 H-Cys-Tyr-Cys-Gln을 코드화하는 뉴클레오티드 서열이며, R은 아미노산 서열 5 내지 127 또는 이 아미노산 서열의 변형체를 코드화하는 뉴클레오티드 서열이고, Y는 아미노산 서열 128내지 146또는 이 아미노산 서열의 변형체를 코드화하는 뉴클레오티드 서열이며, Z는 하나 또는 2개의 정지코돈을 나타낸다.
- 제1항에 있어서, Z가 2개의 정지코돈을 나타내는 방법.
- 제1항에 있어서, 숙주미생물이 이. 콜라이인 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEP3414831.0 | 1984-04-19 | ||
DE93414831.0 | 1984-04-19 | ||
DE19843414831 DE3414831A1 (de) | 1984-04-19 | 1984-04-19 | Herstellung von polypeptiden mit human-gammainterferon-aktivitaet |
Publications (2)
Publication Number | Publication Date |
---|---|
KR850007274A true KR850007274A (ko) | 1985-12-02 |
KR920009505B1 KR920009505B1 (ko) | 1992-10-17 |
Family
ID=6234006
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019850002625A KR920009505B1 (ko) | 1984-04-19 | 1985-04-18 | 인체 γ-인터페론 활성을 갖는 폴리펩타이드의 제조방법 |
Country Status (20)
Country | Link |
---|---|
US (1) | US4832959A (ko) |
EP (1) | EP0161504B1 (ko) |
JP (2) | JPH0745516B2 (ko) |
KR (1) | KR920009505B1 (ko) |
AT (1) | ATE69241T1 (ko) |
CA (1) | CA1339892C (ko) |
DE (2) | DE3414831A1 (ko) |
DK (1) | DK164940C (ko) |
ES (1) | ES542337A0 (ko) |
FI (1) | FI90436C (ko) |
GR (1) | GR850941B (ko) |
HU (1) | HU210111B (ko) |
IE (1) | IE59460B1 (ko) |
IL (1) | IL74947A (ko) |
MA (1) | MA20411A1 (ko) |
NO (1) | NO171983C (ko) |
NZ (1) | NZ211827A (ko) |
PH (1) | PH25251A (ko) |
PT (1) | PT80300B (ko) |
ZA (1) | ZA852900B (ko) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3409966A1 (de) * | 1984-03-19 | 1985-09-26 | Hoechst Ag, 6230 Frankfurt | Gentechnologisches verfahren zur herstellung von human-gammainterferon und mittel zur durchfuehrung dieses verfahrens |
DE3536939A1 (de) * | 1985-10-17 | 1987-04-23 | Hoechst Ag | Biologisch aktive derivate des human-(gamma)-interferons, ihre herstellung und arzneimittel, die solche derivate enthalten |
GB8619725D0 (en) * | 1986-08-13 | 1986-09-24 | Hoffmann La Roche | Homogenous interferon fragments |
DE3642096A1 (de) * | 1986-12-10 | 1988-06-16 | Boehringer Ingelheim Int | Pferde-(gamma)-interferon |
US5157004A (en) * | 1986-12-27 | 1992-10-20 | Takeda Chemical Industries, Ltd. | Polypeptides and production thereof |
JP2653061B2 (ja) * | 1986-12-27 | 1997-09-10 | 武田薬品工業株式会社 | 新規ポリペプチドおよびその製造法 |
CA2390292A1 (en) | 1999-11-12 | 2001-05-25 | Maxygen Holdings Ltd. | Interferon gamma conjugates |
CN1309423C (zh) | 1999-11-12 | 2007-04-11 | 马克西根控股公司 | 干扰素γ偶联物 |
US6958388B2 (en) | 2001-04-06 | 2005-10-25 | Maxygen, Aps | Interferon gamma polypeptide variants |
US7038015B2 (en) * | 2001-04-06 | 2006-05-02 | Maxygen Holdings, Ltd. | Interferon gamma polypeptide variants |
AU2003239774A1 (en) * | 2002-07-03 | 2004-01-23 | Maxygen Holdings Ltd. | Full-length interferon gamma polypeptide variants |
US20050003533A1 (en) | 2003-05-08 | 2005-01-06 | Pawel Kalinski | Mature type-1 polarized dendritic cells with enhanced IL-12 production and methods of serum-free production and use |
BG66458B1 (bg) | 2005-03-21 | 2014-10-31 | Иван Иванов | Средство за конкурентно инхибиране на ендогенен гама интерферон |
CA2607806A1 (en) * | 2005-05-04 | 2006-11-16 | Nautilus Biotech, S.A. | Modified interferon-gamma polypeptides and methods for using modified interferon-gamma polypeptides |
WO2008121461A2 (en) * | 2007-02-26 | 2008-10-09 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Vaccine for activating helper function of cd8+ tcells |
US9359421B2 (en) | 2008-04-08 | 2016-06-07 | Tigo Gmbh | Suppressor of the endogenous interferon-gamma |
BG66517B1 (bg) * | 2008-04-08 | 2016-02-29 | Tigo Gmbh | Супресор на ендогенния човешки гама - интерферон |
JP4821891B2 (ja) * | 2009-07-01 | 2011-11-24 | トヨタ自動車株式会社 | 電池制御システム及び車両 |
BG67190B1 (bg) | 2017-03-29 | 2020-11-16 | Tigo Gmbh | Анти-гама мутантен протеин срещу ендогенния човешки гама интерферон |
WO2020076970A1 (en) | 2018-10-11 | 2020-04-16 | Inhibrx, Inc. | B7h3 single domain antibodies and therapeutic compositions thereof |
US20210380679A1 (en) | 2018-10-11 | 2021-12-09 | Inhibrx, Inc. | Dll3 single domain antibodies and therapeutic compositions thereof |
WO2020077257A1 (en) | 2018-10-11 | 2020-04-16 | Inhibrx, Inc. | Pd-1 single domain antibodies and therapeutic compositions thereof |
US20210340273A1 (en) | 2018-10-11 | 2021-11-04 | Inhlbrx, inc. | 5t4 single domain antibodies and therapeutic compositions thereof |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU561343B2 (en) * | 1981-10-19 | 1987-05-07 | Genentech Inc. | Human immune interferon by recombinant dna |
JPS58110548A (ja) * | 1981-12-24 | 1983-07-01 | Asahi Chem Ind Co Ltd | 新規な生理活性ペプチド |
ZA831094B (en) * | 1982-02-22 | 1983-11-30 | Biogen Nv | Dna sequences,recombinant dna molecules and processes for producing human immune interferon-like polypeptides |
IL68100A0 (en) * | 1982-03-24 | 1983-06-15 | Takeda Chemical Industries Ltd | Novel dna and use thereof |
US6936694B1 (en) * | 1982-05-06 | 2005-08-30 | Intermune, Inc. | Manufacture and expression of large structural genes |
JPH0787797B2 (ja) * | 1982-05-20 | 1995-09-27 | サントリー株式会社 | ヒト・ガンマ・インタ−フエロン様ポリペプチドの製造法 |
US4681848A (en) * | 1982-09-22 | 1987-07-21 | Takeda Chemical Industries, Ltd. | Novel peptide and use thereof |
US4604284A (en) * | 1983-09-20 | 1986-08-05 | Hoffmann-La Roche Inc. | Homogeneous immune interferon fragment |
US4855238A (en) * | 1983-12-16 | 1989-08-08 | Genentech, Inc. | Recombinant gamma interferons having enhanced stability and methods therefor |
-
1984
- 1984-04-19 DE DE19843414831 patent/DE3414831A1/de not_active Withdrawn
-
1985
- 1985-03-04 US US06/707,554 patent/US4832959A/en not_active Expired - Lifetime
- 1985-04-13 EP EP85104501A patent/EP0161504B1/de not_active Expired - Lifetime
- 1985-04-13 AT AT85104501T patent/ATE69241T1/de not_active IP Right Cessation
- 1985-04-13 DE DE8585104501T patent/DE3584579D1/de not_active Expired - Fee Related
- 1985-04-15 HU HU851393A patent/HU210111B/hu not_active IP Right Cessation
- 1985-04-17 IL IL7494785A patent/IL74947A/xx not_active IP Right Cessation
- 1985-04-17 ES ES542337A patent/ES542337A0/es active Granted
- 1985-04-17 PT PT80300A patent/PT80300B/pt unknown
- 1985-04-17 NZ NZ211827A patent/NZ211827A/xx unknown
- 1985-04-17 GR GR850941A patent/GR850941B/el not_active IP Right Cessation
- 1985-04-17 FI FI851529A patent/FI90436C/fi not_active IP Right Cessation
- 1985-04-18 MA MA20635A patent/MA20411A1/fr unknown
- 1985-04-18 IE IE99585A patent/IE59460B1/en not_active IP Right Cessation
- 1985-04-18 JP JP60081502A patent/JPH0745516B2/ja not_active Expired - Lifetime
- 1985-04-18 KR KR1019850002625A patent/KR920009505B1/ko not_active IP Right Cessation
- 1985-04-18 DK DK176285A patent/DK164940C/da not_active IP Right Cessation
- 1985-04-18 ZA ZA852900A patent/ZA852900B/xx unknown
- 1985-04-18 CA CA000479511A patent/CA1339892C/en not_active Expired - Fee Related
- 1985-04-18 NO NO851564A patent/NO171983C/no not_active IP Right Cessation
- 1985-04-18 PH PH32148A patent/PH25251A/en unknown
-
1993
- 1993-09-01 JP JP5217335A patent/JPH07100037B2/ja not_active Expired - Lifetime
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