KR20230034198A - 종양 침윤 림프구의 활성화 및 확장 방법 - Google Patents

종양 침윤 림프구의 활성화 및 확장 방법 Download PDF

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KR20230034198A
KR20230034198A KR1020227030600A KR20227030600A KR20230034198A KR 20230034198 A KR20230034198 A KR 20230034198A KR 1020227030600 A KR1020227030600 A KR 1020227030600A KR 20227030600 A KR20227030600 A KR 20227030600A KR 20230034198 A KR20230034198 A KR 20230034198A
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tils
population
culture medium
cytokine
cell
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Korean (ko)
Inventor
미카 벤슨
노아 제이콥 투보
니콜라스 존 콜레티
로버트 앤드류 라모트
그레고리 브이 크류코프
마이클 슐라바흐
션 필립 리어리 아를라우카스
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케이에스큐 세러퓨틱스 인코포레이티드
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Publication of KR20230034198A publication Critical patent/KR20230034198A/ko
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A—HUMAN NECESSITIES
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    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A—HUMAN NECESSITIES
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    • A61K38/00—Medicinal preparations containing peptides
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    • A61K38/19—Cytokines; Lymphokines; Interferons
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/428—Undefined tumor antigens, e.g. tumor lysate or antigens targeted by cells isolated from tumor
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2121/00—Preparations for use in therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/46—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the cancer treated
    • A61K2239/57—Skin; melanoma
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    • C12N2501/2302—Interleukin-2 (IL-2)
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2502/30—Coculture with; Conditioned medium produced by tumour cells
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  • Animal Behavior & Ethology (AREA)
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  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
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  • Physics & Mathematics (AREA)
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  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020227030600A 2020-02-28 2021-02-26 종양 침윤 림프구의 활성화 및 확장 방법 Pending KR20230034198A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202062983416P 2020-02-28 2020-02-28
US62/983,416 2020-02-28
US202063074841P 2020-09-04 2020-09-04
US63/074,841 2020-09-04
US202163144853P 2021-02-02 2021-02-02
US63/144,853 2021-02-02
PCT/US2021/019861 WO2021173964A1 (en) 2020-02-28 2021-02-26 Methods for activation and expansion of tumor infiltrating lymphocytes

Publications (1)

Publication Number Publication Date
KR20230034198A true KR20230034198A (ko) 2023-03-09

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KR1020227030600A Pending KR20230034198A (ko) 2020-02-28 2021-02-26 종양 침윤 림프구의 활성화 및 확장 방법

Country Status (8)

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US (1) US20230108584A1 (https=)
EP (1) EP4110352A4 (https=)
JP (1) JP2023516300A (https=)
KR (1) KR20230034198A (https=)
CN (1) CN116096865A (https=)
AU (1) AU2021228701A1 (https=)
CA (1) CA3168932A1 (https=)
WO (1) WO2021173964A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109536444B (zh) * 2018-12-11 2022-06-28 吉林省拓华生物科技有限公司 一种适用于恶性实体瘤肿瘤浸润t淋巴细胞的分离诱导方法
CA3158133A1 (en) 2020-04-28 2021-11-04 Lyell Immunopharma, Inc. Methods for culturing cells
EP4239059A4 (en) 2020-11-19 2024-05-15 Suzhou Grit Biotechnology Co., Ltd. METHOD FOR CULTIVATION OF TUMOR INFILTRATING LYMPHOCYTES AND USE THEREOF
US12144827B2 (en) 2021-02-25 2024-11-19 Lyell Immunopharma, Inc. ROR1 targeting chimeric antigen receptor
CN117043321A (zh) * 2021-04-23 2023-11-10 苏州沙砾生物科技有限公司 一种修饰的肿瘤浸润淋巴细胞及其用途
JP2024547000A (ja) * 2021-12-17 2024-12-26 インスティル バイオ インコーポレイテッド 腫瘍浸潤リンパ球の処理
CN118414422A (zh) * 2021-12-30 2024-07-30 苏州沙砾生物科技有限公司 一种修饰的肿瘤浸润淋巴细胞及其用途
WO2023143515A1 (zh) * 2022-01-29 2023-08-03 苏州沙砾生物科技有限公司 一种修饰的肿瘤浸润淋巴细胞及其用途
WO2024020531A1 (en) * 2022-07-21 2024-01-25 Tract Therapeutics, Inc. Immune cell expansion and uses thereof
EP4694917A1 (en) * 2023-04-12 2026-02-18 Beam Therapeutics Inc. Modified immune effector cells with improved efficacy
CN117417894A (zh) * 2023-09-15 2024-01-19 毕诺济(上海)生物技术有限公司 敲除Roquin-1和/或Regnase-1基因的肿瘤浸润淋巴细胞及其应用
WO2025092690A1 (zh) * 2023-10-30 2025-05-08 苏州沙砾生物科技有限公司 一种修饰的细胞及其用途
WO2025096638A2 (en) * 2023-10-30 2025-05-08 Turnstone Biologics Corp. Genetically modified tumor infilitrating lymphocytes and methods of producing and using the same
WO2025106513A1 (en) * 2023-11-13 2025-05-22 KSQ Therapeutics, Inc. Methods for producing edited tumor infiltrating lymphocytes

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2711418B1 (en) * 2012-09-25 2017-08-23 Miltenyi Biotec GmbH Method for polyclonal stimulation of T cells by flexible nanomatrices
EP2961415B1 (en) * 2013-03-01 2021-01-06 The United States of America, as represented by The Secretary, Department of Health and Human Services Methods of producing enriched populations of tumor-reactive t cells from tumor
WO2015157636A1 (en) * 2014-04-10 2015-10-15 H. Lee Moffitt Cancer Center And Research Institute, Inc. Enhanced expansion of tumor-infiltrating lymphocytes for adoptive cell therapy
EP3322424B1 (en) * 2015-07-16 2023-10-11 Yeda Research and Development Co., Ltd. Use of anti third party central memory t cells
KR20180063320A (ko) * 2015-10-20 2018-06-11 카이트 파마 인코포레이티드 T 세포 요법을 위한 t 세포를 제조하는 방법
MX2019007963A (es) * 2017-01-06 2019-10-21 Iovance Biotherapeutics Inc Expansion de linfocitos infiltrantes de tumor (til) con agonistas de la superfamilia de recptor de factor de necrosis tumoral (tnfrsf) y combinaciones terapeuticas de til- y agonistas de tnfrsf.
KR20200003913A (ko) * 2017-05-10 2020-01-10 이오반스 바이오테라퓨틱스, 인크. 액상 종양으로부터의 종양 침윤 림프구의 확장 및 그의 치료 용도
CR20200251A (es) * 2017-11-17 2020-07-17 Iovance Biotherapeutics Inc Expansión de til de aspirados con aguja fina y biopsias por punción
US20190284553A1 (en) * 2018-03-15 2019-09-19 KSQ Therapeutics, Inc. Gene-regulating compositions and methods for improved immunotherapy

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Publication number Publication date
EP4110352A4 (en) 2024-04-24
CN116096865A (zh) 2023-05-09
US20230108584A1 (en) 2023-04-06
AU2021228701A1 (en) 2022-09-15
JP2023516300A (ja) 2023-04-19
EP4110352A1 (en) 2023-01-04
CA3168932A1 (en) 2021-09-02
WO2021173964A1 (en) 2021-09-02

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