KR20200138089A - 5-아미노레불린산 합성 효소 변이체 및 이의 숙주 세포와 용도 - Google Patents

5-아미노레불린산 합성 효소 변이체 및 이의 숙주 세포와 용도 Download PDF

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KR20200138089A
KR20200138089A KR1020200065769A KR20200065769A KR20200138089A KR 20200138089 A KR20200138089 A KR 20200138089A KR 1020200065769 A KR1020200065769 A KR 1020200065769A KR 20200065769 A KR20200065769 A KR 20200065769A KR 20200138089 A KR20200138089 A KR 20200138089A
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South Korea
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ala
amino acid
synthase
acid sequence
ala synthase
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KR1020200065769A
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Korean (ko)
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지빈 쉰
지우주 천
핑 정
청카오 주
수안 구오
원주안 주
얀허 마
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톈진 인스티튜트 오브 인더스트리얼 바이오테크놀로지, 차이니즈 아카데미 오브 사이언시스
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Publication of KR20200138089A publication Critical patent/KR20200138089A/ko

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    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
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    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01037—5-Aminolevulinate synthase (2.3.1.37)
KR1020200065769A 2019-05-31 2020-06-01 5-아미노레불린산 합성 효소 변이체 및 이의 숙주 세포와 용도 KR20200138089A (ko)

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CN201910473377.1 2019-05-31
CN201910473377 2019-05-31

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KR20200138089A true KR20200138089A (ko) 2020-12-09

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JP (1) JP2020195375A (ja)
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CN (1) CN112011524B (ja)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6916971B1 (en) * 1999-07-30 2005-07-12 Rebecca E. Cahoon Polynucleotides encoding aminolevulinic acid biosynthetic enzymes
WO2011127933A1 (en) * 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
CN108251396B (zh) * 2018-03-08 2022-04-01 中国科学院天津工业生物技术研究所 5-氨基乙酰丙酸合成酶突变体及其宿主细胞和应用

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JP2020195375A (ja) 2020-12-10
CN112011524B (zh) 2023-03-21

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