KR20190127983A - 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출 - Google Patents

시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출 Download PDF

Info

Publication number
KR20190127983A
KR20190127983A KR1020197032635A KR20197032635A KR20190127983A KR 20190127983 A KR20190127983 A KR 20190127983A KR 1020197032635 A KR1020197032635 A KR 1020197032635A KR 20197032635 A KR20197032635 A KR 20197032635A KR 20190127983 A KR20190127983 A KR 20190127983A
Authority
KR
South Korea
Prior art keywords
target polynucleotide
sample
amplification
nanopores
payload
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
KR1020197032635A
Other languages
English (en)
Korean (ko)
Inventor
트레버 제이. 모린
타일러 슈롭셔
윌리엄 비. 던바
대니얼 에이. 헬러
홍연 왕
Original Assignee
투 포어 가이즈, 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 투 포어 가이즈, 인코포레이티드 filed Critical 투 포어 가이즈, 인코포레이티드
Publication of KR20190127983A publication Critical patent/KR20190127983A/ko
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020197032635A 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출 Withdrawn KR20190127983A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562111075P 2015-02-02 2015-02-02
US62/111,075 2015-02-02
PCT/US2016/016233 WO2016126746A1 (en) 2015-02-02 2016-02-02 Nanopore detection of target polynucleotides from sample background

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020177024505A Division KR20170106487A (ko) 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출

Publications (1)

Publication Number Publication Date
KR20190127983A true KR20190127983A (ko) 2019-11-13

Family

ID=56564617

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020197032635A Withdrawn KR20190127983A (ko) 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출
KR1020177024505A Ceased KR20170106487A (ko) 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020177024505A Ceased KR20170106487A (ko) 2015-02-02 2016-02-02 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출

Country Status (12)

Country Link
US (1) US11098348B2 (https=)
EP (1) EP3253889A4 (https=)
JP (2) JP2018505414A (https=)
KR (2) KR20190127983A (https=)
CN (1) CN107250380A (https=)
AU (3) AU2016215453A1 (https=)
CA (1) CA2973753A1 (https=)
HK (1) HK1245348A1 (https=)
IL (1) IL253272B (https=)
MX (1) MX2017009766A (https=)
RU (1) RU2017127990A (https=)
WO (1) WO2016126746A1 (https=)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8870950B2 (en) 2009-12-08 2014-10-28 Mitral Tech Ltd. Rotation-based anchoring of an implant
US11653910B2 (en) 2010-07-21 2023-05-23 Cardiovalve Ltd. Helical anchor implantation
US20150351906A1 (en) 2013-01-24 2015-12-10 Mitraltech Ltd. Ventricularly-anchored prosthetic valves
WO2014182634A1 (en) 2013-05-06 2014-11-13 Two Pore Guys, Inc. A method of biological target detection using a nanopore and a fusion protein binding agent
KR102245192B1 (ko) 2013-05-06 2021-04-29 온테라 인크. 나노포어를 이용한 표적 검출
EP3174502B1 (en) 2014-07-30 2022-04-06 Cardiovalve Ltd Apparatus for implantation of an articulatable prosthetic valve
JP6702951B2 (ja) * 2014-09-26 2020-06-03 オンテラ インコーポレイテッド 合成プローブのナノ細孔センシングによる標的配列検出
EP3253333B1 (en) 2015-02-05 2024-04-03 Cardiovalve Ltd Prosthetic valve with axially-sliding frames
JP2018537673A (ja) * 2015-11-23 2018-12-20 ツー ポア ガイズ インコーポレイテッド 追跡及び検出のための標的修飾
US10531866B2 (en) 2016-02-16 2020-01-14 Cardiovalve Ltd. Techniques for providing a replacement valve and transseptal communication
US10640822B2 (en) 2016-02-29 2020-05-05 Iridia, Inc. Systems and methods for writing, reading, and controlling data stored in a polymer
US10438662B2 (en) 2016-02-29 2019-10-08 Iridia, Inc. Methods, compositions, and devices for information storage
US10859562B2 (en) 2016-02-29 2020-12-08 Iridia, Inc. Methods, compositions, and devices for information storage
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
US20190231525A1 (en) 2016-08-01 2019-08-01 Mitraltech Ltd. Minimally-invasive delivery systems
ES3018641T3 (es) 2016-08-10 2025-05-16 Cardiovalve Ltd Válvula protésica con marcos concéntricos
AU2017348009B2 (en) 2016-10-24 2019-10-10 Ontera Inc. Fractional abundance of polynucleotide sequences in a sample
KR102076592B1 (ko) * 2017-05-25 2020-02-13 가천대학교 산학협력단 나노기공 센서를 사용한 dna 농도 측정 장치 및 방법
US11793633B2 (en) 2017-08-03 2023-10-24 Cardiovalve Ltd. Prosthetic heart valve
US10888421B2 (en) 2017-09-19 2021-01-12 Cardiovalve Ltd. Prosthetic heart valve with pouch
US12064347B2 (en) 2017-08-03 2024-08-20 Cardiovalve Ltd. Prosthetic heart valve
US12458493B2 (en) 2017-09-19 2025-11-04 Cardiovalve Ltd. Prosthetic heart valve and delivery systems and methods
CN108181358B (zh) * 2017-11-15 2020-02-11 华东理工大学 基于气单胞菌溶素纳米通道检测氨基酸的方法
CN109239140B (zh) * 2018-08-16 2021-04-13 广东第二师范学院 一种纳米孔功能性控制方法及系统
CN113966403A (zh) * 2019-04-22 2022-01-21 奥特拉公司 样品中多核苷酸序列的分数丰度的多孔测定
US20200340943A1 (en) * 2019-04-26 2020-10-29 The University Of Ottawa Calibrating Nanopore Capture Rates Using Controlled Counting
CN110438203B (zh) * 2019-08-19 2023-11-28 上海纳米技术及应用国家工程研究中心有限公司 一种提高环介导等温扩增反应效率的方法及应用
JP7344786B2 (ja) * 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のｄｎａ配列を同定する方法
KR102358418B1 (ko) * 2019-12-31 2022-02-04 주식회사 제노헬릭스 스몰 rna 검출 방법
WO2021156370A1 (en) * 2020-02-06 2021-08-12 F. Hoffmann-La Roche Ag Compositions that reduce template threading into a nanopore
US11837302B1 (en) 2020-08-07 2023-12-05 Iridia, Inc. Systems and methods for writing and reading data stored in a polymer using nano-channels
US12357459B2 (en) 2020-12-03 2025-07-15 Cardiovalve Ltd. Transluminal delivery system

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05273209A (ja) 1992-03-25 1993-10-22 Takara Shuzo Co Ltd Ｄｎａ検出用キット
JPH11346798A (ja) 1998-06-04 1999-12-21 Makoto Tsuruoka 核酸の測定方法およびＶｅｒｏ毒素生産菌の検出方法
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
AU2002359510A1 (en) * 2001-11-30 2003-06-17 Nanosphere, Inc. Real-time monitoring of pcr amplification using nanoparticle probes
US6952651B2 (en) 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
WO2004001379A2 (en) 2002-06-20 2003-12-31 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US7270956B2 (en) 2002-08-26 2007-09-18 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
US20040022764A1 (en) 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
DE602004030114D1 (de) 2003-05-20 2010-12-30 Investigen Inc System zum nachweis von polynukleotiden
US20050186576A1 (en) 2004-02-19 2005-08-25 Intel Corporation Polymer sequencing using selectively labeled monomers and data integration
TWI291416B (en) * 2005-07-19 2007-12-21 Benq Corp Business equipment
KR100691806B1 (ko) * 2005-08-04 2007-03-12 삼성전자주식회사 비드 및 나노포어를 이용한 핵산 검출방법 및 검출장치
US20070048745A1 (en) 2005-08-30 2007-03-01 Joyce Timothy H Systems and methods for partitioned nanopore analysis of polymers
US20070190543A1 (en) * 2005-11-14 2007-08-16 Applera Corporation Coded Molecules for Detecting Target Analytes
WO2007117832A2 (en) * 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
US8889348B2 (en) 2006-06-07 2014-11-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by nanopore using modified nucleotides
AU2008236694B2 (en) 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US20080268440A1 (en) * 2007-04-26 2008-10-30 Liu Timothy Z Biomolecule immobilization on surface via hydrophobic interactions
GB0717150D0 (en) 2007-09-04 2007-10-17 Univ Warwick Apparatus and method
US8329647B2 (en) * 2008-07-17 2012-12-11 Ikfe Institut Fur Klinische Forschung Und Entwicklung Gmbh Method of treating a subject according to biomarkers for insulin resistance
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
AU2010301128B2 (en) 2009-09-30 2014-09-18 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
KR101267789B1 (ko) 2010-06-25 2013-06-04 서울대학교산학협력단 나노 포어 구조를 이용한 ｄｎａ 분석용 장치, 분석 방법 및 ｐｃｒ 정량 검출 장치
WO2012088339A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization using speed bumps
WO2012135658A2 (en) 2011-03-30 2012-10-04 Noblegen Biosciences, Inc. Sequence preserved dna conversion for optical nanopore sequencing
US9926596B2 (en) * 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP2734840B3 (en) 2011-07-20 2017-06-07 The Regents of the University of California Dual-pore device
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
EP3674412A1 (en) * 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
WO2014024041A1 (en) 2012-08-06 2014-02-13 Quantumdx Group Limited Method and kit for nucleic acid sequencing
US20140099726A1 (en) 2012-10-10 2014-04-10 Two Pore Guys, Inc. Device for characterizing polymers
US9732379B2 (en) 2013-03-15 2017-08-15 The Curators Of The University Of Missouri Encoded nanopore sensor for multiplex nucleic acids detection
WO2014182634A1 (en) 2013-05-06 2014-11-13 Two Pore Guys, Inc. A method of biological target detection using a nanopore and a fusion protein binding agent
US20150011925A1 (en) * 2013-07-08 2015-01-08 Robert F. Buckman, Jr. Method and device for temporary emergency vessel anastomoses
CN107110817B (zh) * 2014-12-31 2019-09-24 卡尤迪生物科技（北京）有限公司 使用纳米孔和标签的核酸分子检测
CA2986921A1 (en) 2015-06-02 2016-12-08 Nanopore Diagnostics, Llc Nucleic acid detection
WO2018093976A1 (en) 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag

Also Published As

Publication number Publication date
WO2016126746A1 (en) 2016-08-11
EP3253889A4 (en) 2018-09-26
AU2016215453A1 (en) 2017-08-10
JP2019092509A (ja) 2019-06-20
MX2017009766A (es) 2017-12-11
AU2020239812A1 (en) 2020-10-29
RU2017127990A3 (https=) 2019-03-04
US20180023115A1 (en) 2018-01-25
RU2017127990A (ru) 2019-03-04
KR20170106487A (ko) 2017-09-20
HK1245348A1 (zh) 2018-08-24
US11098348B2 (en) 2021-08-24
CA2973753A1 (en) 2016-08-11
IL253272B (en) 2018-08-30
AU2018208656A1 (en) 2018-08-09
EP3253889A1 (en) 2017-12-13
IL253272A0 (en) 2017-09-03
CN107250380A (zh) 2017-10-13
JP2018505414A (ja) 2018-02-22

Similar Documents

Publication Publication Date Title
KR20190127983A (ko) 시료 배경으로부터의 표적 폴리뉴클레오티드의 나노포어 검출
JP6541114B2 (ja) ナノポアによる標的検出法
JP7610051B2 (ja) ナノ細孔デバイス内で特徴をマッピングするための強制移動ポリヌクレオチドスキャンニング
CN106471369B (zh) 利用纳米细孔的目标检测
King et al. Screening for group a streptococcal disease via solid-state nanopore detection of pcr amplicons
JP2019516087A (ja) 断片化及びペイロード結合による試料バックグラウンドからの標的ポリヌクレオチドのナノ細孔識別
EP2867369A2 (en) Method and devices for detecting macroions in a liquid medium
JP6630672B2 (ja) Ｎｇｓシステム用の対照及びそれを用いる方法
Loh et al. Electric single-molecule hybridization detector for short DNA fragments
JP2021536261A (ja) ナノ細孔デバイスを使用して分子を検出するためのセンシング組成物、方法、およびデバイス
Dong et al. Addressing Buffer, Size, and Clogging Challenges in LAMP-Coupled Solid-State Nanopores for Point-of-Care Testing
US20200024650A1 (en) Target Polynucleotide Detection and Sequencing by Incorporation of Modified Nucleotides for Nanaopore Analysis
King Nanopore Sensing of PCR-Amplified Pathogenic DNA
JP2021097703A (ja) 鋳型核酸の分析方法、標的物質の分析方法、鋳型核酸または標的物質の分析用キット、および鋳型核酸または標的物質の分析用装置
HK1235090A1 (en) Target sequence detection by nanopore sensing of synthetic probes
AU2015259187A1 (en) Selective analysis of modified biological molecules with solid-state nanopores
HK1234825A1 (en) Target detection with nanopore

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20191104

Application number text: 1020177024505

Filing date: 20170831

PG1501 Laying open of application
PC1203 Withdrawal of no request for examination