KR20180055905A - 증폭 산물의 농축을 위한 방법 및 조성물 - Google Patents
증폭 산물의 농축을 위한 방법 및 조성물 Download PDFInfo
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- KR20180055905A KR20180055905A KR1020187013081A KR20187013081A KR20180055905A KR 20180055905 A KR20180055905 A KR 20180055905A KR 1020187013081 A KR1020187013081 A KR 1020187013081A KR 20187013081 A KR20187013081 A KR 20187013081A KR 20180055905 A KR20180055905 A KR 20180055905A
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- sequence
- primer
- target polynucleotide
- polynucleotide
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/307—Circular oligonucleotides
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- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
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- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
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- C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
- C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
- C12Q2533/101—Primer extension
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- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Evolutionary Biology (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
- Macromonomer-Based Addition Polymer (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562239690P | 2015-10-09 | 2015-10-09 | |
| US62/239,690 | 2015-10-09 | ||
| PCT/US2016/056126 WO2017062863A1 (en) | 2015-10-09 | 2016-10-07 | Methods and compositions for enrichment of amplification products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20180055905A true KR20180055905A (ko) | 2018-05-25 |
Family
ID=58488563
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020187013081A Pending KR20180055905A (ko) | 2015-10-09 | 2016-10-07 | 증폭 산물의 농축을 위한 방법 및 조성물 |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US10752942B2 (enExample) |
| EP (1) | EP3359693B1 (enExample) |
| JP (3) | JP7008016B2 (enExample) |
| KR (1) | KR20180055905A (enExample) |
| CN (2) | CN108368545B (enExample) |
| AU (2) | AU2016334233B2 (enExample) |
| CA (1) | CA2999902A1 (enExample) |
| HK (1) | HK1259101A1 (enExample) |
| IL (2) | IL258236B (enExample) |
| MX (2) | MX2018004284A (enExample) |
| SG (1) | SG11201802864RA (enExample) |
| WO (1) | WO2017062863A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102102107B1 (ko) * | 2019-05-30 | 2020-04-20 | 단국대학교 천안캠퍼스 산학협력단 | 이동성 유전인자 SINE 선택적 발굴을 위한 HiSeq 시퀀서 기반의 DNA 라이브러리 제작 방법 |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102379877B1 (ko) | 2013-12-11 | 2022-03-30 | 아큐라젠 홀딩스 리미티드 | 희귀 서열 변이를 검출하기 위한 조성물 및 방법 |
| WO2017062863A1 (en) | 2015-10-09 | 2017-04-13 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| US11286519B2 (en) | 2013-12-11 | 2022-03-29 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| US11859246B2 (en) | 2013-12-11 | 2024-01-02 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| US12163184B2 (en) * | 2015-12-03 | 2024-12-10 | Accuragen Holdings Limited | Methods and compositions for forming ligation products |
| WO2017201102A1 (en) | 2016-05-16 | 2017-11-23 | Accuragen Holdings Limited | Method of improved sequencing by strand identification |
| AU2017290237B2 (en) | 2016-06-30 | 2020-10-22 | GRAIL, Inc | Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library |
| SG11201901296TA (en) | 2016-08-15 | 2019-03-28 | Accuragen Holdings Ltd | Compositions and methods for detecting rare sequence variants |
| CN107177693B (zh) * | 2017-07-19 | 2020-11-06 | 中山大学 | 一种多倍pcr扩增方法 |
| CN107760763B (zh) * | 2017-11-20 | 2021-05-11 | 东南大学 | 一种用于茎环引物-滚环扩增反应的茎环引物以及茎环引物-滚环扩增的应用 |
| CN109971825B (zh) * | 2017-12-28 | 2020-11-10 | 南京金斯瑞生物科技有限公司 | 快速制备桑格测序模板的方法 |
| US11203782B2 (en) * | 2018-03-29 | 2021-12-21 | Accuragen Holdings Limited | Compositions and methods comprising asymmetric barcoding |
| CN112601823A (zh) | 2018-06-12 | 2021-04-02 | 安可济控股有限公司 | 用于形成连接产物的方法和组合物 |
| EP3650558A1 (en) * | 2018-11-07 | 2020-05-13 | Siemens Healthcare GmbH | Liquid sample workflow for nanopore sequencing |
| CN110820051B (zh) * | 2018-12-28 | 2023-04-28 | 广州表观生物科技有限公司 | 一种高灵敏度融合基因检测方法及其应用 |
| WO2020263978A1 (en) * | 2019-06-25 | 2020-12-30 | Accuragen Holdings Limited | Methods and systems for disease detection |
| GB201911421D0 (en) * | 2019-08-09 | 2019-09-25 | Dnae Diagnostics Ltd | Methods of multiplexed isothermal amplification of nucleic acid sequences |
| WO2021046655A1 (en) * | 2019-09-13 | 2021-03-18 | University Health Network | Detection of circulating tumor dna using double stranded hybrid capture |
| JP2023502138A (ja) | 2019-11-19 | 2023-01-20 | ワン,シャオ・ビン | サークルcDNA増幅によって遺伝子融合を同定するための方法 |
| WO2021127208A1 (en) * | 2019-12-20 | 2021-06-24 | Accuragen Holdings Limited | Methods and systems for disease detection |
| KR102468839B1 (ko) | 2020-06-03 | 2022-11-18 | 주식회사 제노헬릭스 | Rna 검출 방법 |
| AU2021294360A1 (en) * | 2020-06-26 | 2023-02-02 | William Marsh Rice University | Amplicon Comprehensive Enrichment |
| KR20230091116A (ko) * | 2020-10-21 | 2023-06-22 | 일루미나, 인코포레이티드 | 다수의 삽입체를 포함하는 시퀀싱 주형, 및 시퀀싱 처리량을 개선하기 위한 조성물 및 방법 |
| WO2022103750A1 (en) * | 2020-11-11 | 2022-05-19 | Accuragen Holdings Limited | Methods and systems for sample normalization |
| CN113549674B (zh) * | 2021-04-22 | 2023-11-03 | 福建和瑞基因科技有限公司 | 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒 |
| CN113832147B (zh) * | 2021-09-08 | 2024-06-14 | 华南农业大学 | 一种高效的大片段dna合成与扩增的pcr引物、方法及应用 |
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| WO1995013375A1 (en) | 1993-11-10 | 1995-05-18 | The Johns Hopkins University | Tumor suppressor waf1 |
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| DE69507289T2 (de) | 1994-03-15 | 1999-07-08 | Scientific Generics Ltd., Cambridge | Elektrochemische denatusierung von doppelsträngiger nukleinsäure |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102102107B1 (ko) * | 2019-05-30 | 2020-04-20 | 단국대학교 천안캠퍼스 산학협력단 | 이동성 유전인자 SINE 선택적 발굴을 위한 HiSeq 시퀀서 기반의 DNA 라이브러리 제작 방법 |
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| JP2022050563A (ja) | 2022-03-30 |
| AU2016334233B2 (en) | 2023-01-05 |
| IL258236A (en) | 2018-05-31 |
| CN114807323A (zh) | 2022-07-29 |
| MX2024002873A (es) | 2024-03-22 |
| US20230287485A1 (en) | 2023-09-14 |
| IL258236B (en) | 2021-12-01 |
| JP2018537955A (ja) | 2018-12-27 |
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| US10752942B2 (en) | 2020-08-25 |
| AU2023201912A1 (en) | 2023-05-04 |
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